Interference-Free Detection of Hydroxyl Radical and Arthritis Diagnosis by Rare Earth-Based Nanoprobe Utilizing SWIR Emission as Reference

Due to the high oxidative potential of the hydroxyl radical ((OH)-O-center dot), the accumulation of (OH)-O-center dot in tissues can cause inflammation, such as that in arthritis. Therefore, the development of (OH)-O-center dot detection with high efficiency and sensitivity is important for the treatment of related diseases. In this work, a cypate-modified core-shell NaErF4@NaLuF4 nanoprobe (csEr-Cy) was designed for detecting (OH)-O-center dot on the basis of a typical reaction between cypate and (OH)-O-center dot. The process resulted in the recovery of 654 nm upconversion luminescence emission of csEr because of a weakened inner filter effect (IFE) and Forster resonance energy transfer (FRET). The short-wavelength infrared (SWIR) emission at 1550 nm was not affected by (OH)-O-center dot addition, thus enabling interference-free detection. Density functional theory (DFT) calculations were performed to explain the underlying mechanism. With the SWIR signal used as a reference for (OH)-O-center dot detection, the csEr-Cy nanoprobe showed higher sensitivity and penetration than visible reference. This method was successfully used in mice for the diagnosis of arthritis in vivo. Our results provide novel insights into improving the sensitivity of nanoprobes for molecule detection and disease diagnosis.