Article
Biochemistry & Molecular Biology
Marta Clos-Sansalvador, Sergio G. Garcia, Miriam Moron-Font, Charles Williams, Niels-Christian Reichardt, Juan M. Falcon-Perez, Antoni Bayes-Genis, Santiago Roura, Marcella Franquesa, Marta Monguio-Tortajada, Francesc E. Borras
Summary: This study reveals the importance of N-glycosylation in MSC-EV for their interaction with endothelial cells and associated functions, such as cell uptake, migration, and angiogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Ruhua Luo, Mengmeng Liu, Tiantian Tan, Qian Yang, Yue Wang, Lianhui Men, Liping Zhao, Honghua Zhang, Shuling Wang, Tian Xie, Qingchang Tian
Summary: EVs are membrane-bound vesicles that offer advantages over traditional nanocarriers for drug and gene delivery, with therapeutic potential through chemical or biological modifications. Modified EVs can be used to load various therapeutic agents for disease intervention, providing new avenues for effective treatment strategies.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Chemistry, Analytical
Xiaojuan Liu, Xin Gao, Limin Yang, Yuecan Zhao, Feng Li
Summary: The combination of a metal-organic framework-functionalized paper and a screen-printed electrode has enabled the development of a simple and cost-efficient electrochemical biosensor for detecting cancer-derived exosomes with high sensitivity and a low detection limit. This label-free paper-based biosensor offers advantages such as low cost, ease of operation, and high sensitivity, making it suitable for point-of-care diagnosis in resource-limited settings.
ANALYTICAL CHEMISTRY
(2021)
Review
Oncology
Andres Rincon-Riveros, Liliana Lopez, E. Victoria Villegas, Josefa Antonia Rodriguez
Summary: Extracellular vesicles, such as exosomes, have the potential to serve as biomarkers for cancer development, impacting various aspects of tumor progression and immune regulation.
Review
Pharmacology & Pharmacy
Na Xu, Ranran Guo, Xiaotong Yang, Ning Li, Jia Yu, Peng Zhang
Summary: Exosomes are nanoscale capsules secreted by living cells, playing important roles in material and information exchange between cells and exhibiting both beneficial and unfavorable effects in tumor treatment.
ASIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Yong Weon Yi
Summary: Anticancer drug resistance is a major challenge in cancer treatment, and extracellular vesicles (EVs) derived from cancer cells have been identified as a crucial mechanism for drug resistance, tumor progression, and metastasis. EVs, which consist of a lipid bilayer, can transfer various substances including proteins, nucleic acids, lipids, and metabolites from the originating cell to the recipient cell. The investigation of EVs' role in drug resistance is still at an early stage. This review analyzes the involvement of EVs derived from triple-negative breast cancer cells (TNBC-EVs) in anticancer drug resistance and discusses strategies to overcome TNBC-EV-mediated drug resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Shizhen Shen, Zhangjin Shen, Conghui Wang, Xiaodong Wu, Lingfang Wang, Lele Ye, Songfa Zhang, Xiaodong Cheng
Summary: In this study, a method for extracting extracellular vesicles (EVs) from epithelial ovarian cancer (EOC) tissues was optimized to investigate the effect of cryopreservation on the phenotypes, contents, and biological functions of the extracted EVs. The results showed that cryopreservation affected the yield and distribution of EVs, but not their morphology or particle mass ratio. The storage of tissues at -80°C increased both small and large EV yields, while the storage of lysates at -80°C decreased large EV yield and increased small EV yield. The cryopreserved EOC tissue retained a high percentage of proteins and miRNAs, and the extracted EVs could promote angiogenesis and invasive migration ability in human endothelial cells. This suggests that cryopreserved EOC tissue has clinical value for studying tissue-derived EVs, especially those related to EV proteins.
Review
Oncology
Tianjiao Luo, Juliane Von der Ohe, Ralf Hass
Summary: Therapeutic functions of MSCs are largely mediated through paracrine effects by releasing various components such as cytokines, chemokines, and metabolites, among which exosomes play a significant role in tissue repair and immune modulation. However, MSC-derived exosomes can exhibit both tumor-inhibitory and tumor-supportive effects, depending on their interactions with cancer cells. The microRNA content of exosomes is crucial in determining their specific actions in different tissues and diseases.
Article
Oncology
Elisabetta Vergani, Elena Daveri, Viviana Vallacchi, Laura Bergamaschi, Luca Lalli, Chiara Castelli, Monica Rodolfo, Licia Rivoltini, Veronica Huber
Summary: The impact of extracellular vesicles (EVs) on anti-tumor immune responses is still being explored. The small size and numerous subtypes of EVs, as well as the difficulty in determining their origin, pose challenges for investigation. While the study of cancer EVs has been facilitated by the existence of tumor cell lines, understanding the phenotypes and functions of immune cell EVs in this context is more complex. The emergence of immunotherapy with immune checkpoint inhibitors has increased the need to understand the role of EVs in immune activation and response, particularly in unraveling and preventing resistance in patients.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Chenggong Tu, Zhimin Du, Hui Zhang, Yueyuan Feng, Yujun Qi, Yongjiang Zheng, Jinbao Liu, Jinheng Wang
Summary: Extracellular vesicles derived from bone marrow stromal cells can promote the cycling of multiple myeloma cells and reduce their sensitivity to bortezomib. Inhibiting endocytosis can decrease vesicle uptake by cells and enhance the anti-myeloma effects of bortezomib.
Article
Genetics & Heredity
Alejandro Pando, Christoph Schorl, Loren D. Fast, John L. Reagan
Summary: Extracellular vesicles (EVs), which are lipid bilayer particles containing various substances, are excreted from all cells in the human body. In this study, researchers investigated the effects of acute myeloid leukemia (AML)-derived EVs on gene expression levels in three different T cell populations, finding that these EVs modulate gene expression and significantly impact immunoregulatory pathways.
Review
Cell & Tissue Engineering
Riccardo Bazzoni, Ilaria Tanasi, Nice Turazzi, Mauro Krampera
Summary: Extracellular vesicles (EVs) are released by cells and contain various bioactive molecules, playing a crucial role in intercellular communication and biological processes. In hematological malignancies, EVs contribute to tumor development, drug resistance, and bone marrow microenvironment modulation. EVs have potential as diagnostic biomarkers, therapeutic targets, and drug carriers.
Article
Chemistry, Multidisciplinary
Alvaro M. Martins, Tania M. Lopes, Francisca Diniz, Jose Pires, Hugo Osorio, Filipe Pinto, Daniela Freitas, Celso A. Reis
Summary: Alterations in glycosylation machinery in cancer cells lead to the synthesis of abnormal glycan structures in tumor cells. Extracellular vesicles (EVs) are involved in cancer communication and progression, and certain tumor-associated glycans have been identified in cancer EVs. This study investigates the impact of 3D tumor architecture on the selective packaging of cellular glycans into EVs. The results show differential packaging of specific proteins and glycans in EVs produced by 2D and 3D cultured cells, suggesting distinct biological functions and correlation with clinical data.
Article
Biology
Yang Li, Yan Wu, Elena A. Federzoni, Xiaodan Wang, Andre Dharmawan, Xiaoyi Hu, Hui Wang, Robert J. Hawley, Sean Stevens, Megan Sykes, Yong-Guang Yang
Summary: CD47 cross-dressing provides an alternative source of cell surface CD47 that can elicit its anti-phagocytic function without inducing cell death, suggesting a new approach to optimize CD47 transgenic method.
Review
Biochemistry & Molecular Biology
Theresa Akoto, Sharanjot Saini
Summary: Prostate cancer is a life-threatening disease among men globally, with metastasis being the major cause of mortality. Cell-to-cell communication plays a crucial role in the progression and metastasis of prostate cancer, with exosomes/extracellular vesicles demonstrating significant involvement in signaling pathways related to metastasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Hayato Mizuta, Ayane Takakusaki, Takehiro Suzuki, Keisuke Otake, Naoshi Dohmae, Siro Simizu
Summary: C-mannosylation is a unique type of protein glycosylation that affects protein functions, including secretion, intracellular localization, and stability. This study identifies a new C-mannosylated protein, RAMP1, and demonstrates that C-mannosylation enhances protein stability and cell migration activity.
Article
Biochemistry & Molecular Biology
Satoshi Yoshimoto, Takehiro Suzuki, Naoki Otani, Daisuke Takahashi, Kazunobu Toshima, Naoshi Dohmae, Siro Simizu
Summary: C-mannosylation is a rare type of protein glycosylation that affects protein secretion, intracellular localization, and stability. In this study, the VMO1 protein was found to be C-mannosylated at Trp(105), leading to destabilization and conformational changes in the protein.
Article
Multidisciplinary Sciences
Ryoya Nakagawa, Hisato Hirano, Satoshi N. Omura, Suchita Nety, Soumya Kannan, Han Altae-Tran, Xiao Yao, Yuriko Sakaguchi, Takayuki Ohira, Wen Y. Wu, Hiroshi Nakayama, Yutaro Shuto, Tatsuki Tanaka, Fumiya K. Sano, Tsukasa Kusakizako, Yoshiaki Kise, Yuzuru Itoh, Naoshi Dohmae, John van der Oost, Tsutomu Suzuki, Feng Zhang, Osamu Nureki
Summary: Researchers used cryo-electron microscopy to determine the structure of the complex formed by ISDra2 TnpB, its cognate.RNA, and target DNA. They found that the.RNA had an unexpected structure and formed a pseudoknot, which is a common feature among all guide RNAs of Cas12 enzymes. Furthermore, the structure revealed how the compact TnpB recognized the omega RNA and cleaved the target DNA complementary to the guide. Comparison with Cas12 enzymes suggested that CRISPR-Cas12 effectors acquired the ability to recognize the protospacer-adjacent motif-distal end of the guide RNA-target DNA heteroduplex, possibly through asymmetric dimer formation or diverse REC2 insertions, enabling engagement in CRISPR-Cas adaptive immunity. Overall, this study provides mechanistic insights into TnpB function and advances our understanding of the evolution from transposon-encoded TnpB proteins to CRISPR-Cas12 effectors.
Article
Cell Biology
Yoshihiko Kuchitsu, Kojiro Mukai, Rei Uematsu, Yuki Takaada, Ayumi Shinojima, Ruri Shindo, Tsumugi Shoji, Shiori Hamano, Emari Ogawa, Ryota Sato, Kensuke Miyake, Akihisa Kato, Yasushi Kawaguchi, Masahiko Nishitani-Isa, Kazushi Izawa, Ryuta Nishikomori, Takahiro Yasumi, Takehiro Suzuki, Naoshi Dohmae, Takefumi Uemura, Glen N. N. Barber, Hiroyuki Arai, Satoshi Waguri, Tomohiko Taguchi
Summary: Stimulator of interferon genes (STING) is crucial for the immune response against DNA pathogens, and its degradation is mediated by endosomal sorting complexes required for transport (ESCRT) proteins. STING-positive vesicles derived from recycling endosomes are encapsulated into lysosomes after STING ubiquitination. This study provides insights into the regulation of STING and prevents hyperactivation of the immune response.
NATURE CELL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Kei Fukuda, Takeshi Shimi, Chikako Shimura, Takao Ono, Takehiro Suzuki, Kenta Onoue, Satoko Okayama, Hisashi Miura, Ichiro Hiratani, Kazuho Ikeda, Yasushi Okada, Naoshi Dohmae, Shigenobu Yonemura, Azusa Inoue, Hiroshi Kimura, Yoichi Shinkai
Summary: Heterochromatin, a critical architectural feature of eukaryotic chromosomes, plays crucial roles in cell type-specific gene expression and genome stability. The spatial organization of heterochromatin is poorly understood, and this study investigates the role of H3K9 and H3K27 methylation in heterochromatin organization. The findings demonstrate that the loss of H3K9 methylation leads to the redistribution of H3K27me3 and impaired condensation and spatial organization of heterochromatin in mammalian cells.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Cell Biology
Xian-Yang Qin, Yutaka Furutani, Kento Yonezawa, Nobutaka Shimizu, Miyuki Kato-Murayama, Mikako Shirouzu, Yali Xu, Yumiko Yamano, Akimori Wada, Luc Gailhouste, Rajan Shrestha, Masataka Takahashi, Jeffrey W. W. Keillor, Ting Su, Wenkui Yu, Shinya Fujii, Hiroyuki Kagechika, Naoshi Dohmae, Yohei Shirakami, Masahito Shimizu, Takahiro Masaki, Tomokazu Matsuura, Harukazu Suzuki, Soichi Kojima
Summary: This study examined the effects of ACR on TG2 activity at a structural level and its role in depleting liver cancer stem cells (CSCs). It was found that ACR directly binds to TG2, induces oligomer formation, and inhibits the transamidase activity of cytoplasmic TG2. The loss of TG2 function suppressed stemness-related genes, spheroid proliferation, and induced cell death in a liver CSC subpopulation. Proteome analysis revealed that TG2 inhibition suppressed the expression of EXT1 and heparan sulfate biosynthesis. ACR could act as a novel TG2 inhibitor and TG2-mediated EXT1 signaling is a promising therapeutic target.
CELL DEATH & DISEASE
(2023)
Article
Multidisciplinary Sciences
Ryo Nagao, Koji Kato, Tasuku Hamaguchi, Yoshifumi Ueno, Naoki Tsuboshita, Shota Shimizu, Miyu Furutani, Shigeki Ehira, Yoshiki Nakajima, Keisuke Kawakami, Takehiro Suzuki, Naoshi Dohmae, Seiji Akimoto, Koji Yonekura, Jian-Ren Shen
Summary: By using cryo-EM, the authors determined the structure of a PSI monomer associated with six IsiA proteins in Anabaena, providing insights into the diversity and functions of IsiAs. IsiAs are expressed in cyanobacteria under iron-deficient conditions, and the binding properties and functional roles of IsiAs in PSI were still unknown in Anabaena. The cryo-EM structure of a PSI-IsiA supercomplex isolated from iron-deficient Anabaena revealed the association of six IsiA subunits with a PSI core monomer, and the absence of a PsaL subunit due to the occupation of its position by the C-terminal domain of IsiA2.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Kota Noritsugu, Takehiro Suzuki, Kosuke Dodo, Kenji Ohgane, Yasue Ichikawa, Kota Koike, Satoshi Morita, Takashi Umehara, Kenji Ogawa, Mikiko Sodeoka, Naoshi Dohmae, Minoru Yoshida, Akihiro Ito
Summary: TEAD transcription factors regulate the transcriptional output of Hippo signaling by phosphorylating YAP and TAZ as coactivators. Recent studies have shown that cysteine palmitoylation also plays a role in regulating TEAD activity. This study discovers lysine long-chain fatty acylation as another post-translational modification of TEADs. Lysine fatty acylation enhances the interaction between TEADs and YAP/TAZ, contributing to their transcriptional activity. Interestingly, lysine fatty acylation of TEADs increases upon Hippo signaling activation, despite a decrease in cysteine acylation. These findings provide new insights into the regulation of TEAD activity through fatty-acyl modifications.
Article
Biochemical Research Methods
Hajime Kaga, Hirotoshi Matsumura, Ayano Saito, Masaya Saito, Fumito Abe, Takehiro Suzuki, Naoshi Dohmae, Masafumi Odaka, Atsushi Komatsuda, Hideki Wakui, Naoto Takahashi
Summary: A comparative proteomic analysis of glomerular proteins was performed in IgAN and IgAVN patients, revealing shared molecular mechanisms for glomerular injury in both diseases except for enhanced glomerular complement activation in IgAN.
CLINICAL PROTEOMICS
(2023)
Article
Biochemistry & Molecular Biology
Xuguang Jiang, Tadayuki Ogawa, Kento Yonezawa, Nobutaka Shimizu, Sotaro Ichinose, Takayuki Uchihashi, Wataru Nagaike, Toshio Moriya, Naruhiko Adachi, Masato Kawasaki, Naoshi Dohmae, Toshiya Senda, Nobutaka Hirokawa
Summary: This study reveals the molecular mechanism of specific and dynamic cargo-binding of kinesin complexes. The two-step cargo recognition and stabilization mechanism of kinesins provides novel insights into intracellular trafficking machinery.
Article
Biochemistry & Molecular Biology
Ryota Kawahara, Tomoko Usami, Satoko Arakawa, Hiroki Kamo, Takehiro Suzuki, Ryosuke Komatsu, Hiroyuki Hara, Yuki Niwa, Erina Shimizu, Naoshi Dohmae, Shigeomi Shimizu, Siro Simizu
Summary: PMEL is a melanocyte-specific glycoprotein involved in melanosome maturation. It undergoes C-mannosylation at multiple tryptophan residues in its core amyloid fragment and N-terminal fragment. The C-mannosylation of PMEL is required for proper melanosome development and fibril formation.
Article
Multidisciplinary Sciences
Masaki Kikuchi, Satoshi Morita, Masatoshi Wakamori, Shin Sato, Tomomi Uchikubo-Kamo, Takehiro Suzuki, Naoshi Dohmae, Mikako Shirouzu, Takashi Umehara
Summary: In this study, the authors discovered that p300/CBP recognizes and acetylates histone H4 N-terminal tail (NT) acetylation (ac) in a nucleosome, and also acetylates non-H4 histone NTs within the same nucleosome. The authors propose a model in which p300/CBP replicates histone N-terminal tail acetylation within the H3-H4 tetramer and transfers it to the H2B-H2A dimers to activate context-dependent gene transcription through local nucleosome destabilization.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Kentaro Matsumoto, Shenwei Ni, Hiroyuki Arai, Takashi Toyama, Yoshiro Saito, Takehiro Suzuki, Naoshi Dohmae, Kojiro Mukai, Tomohiko Taguchi
Summary: Phenylarsine oxide (PAO) can activate mouse STING by covalently binding to cysteine residues, independent of cGAS or cGAMP. STING activation with PAO requires ER-to-Golgi trafficking and palmitoylation of STING. The study reveals that cysteine residues in STING can serve as a novel target for the development of STING agonists.
CELL STRUCTURE AND FUNCTION
(2023)
Article
Biochemistry & Molecular Biology
Shun Maruyama, Kota Sawano, Satoko Amaki, Takehiro Suzuki, Satoru Narita, Kenta Kimura, Takatoshi Arakawa, Chihaya Yamada, Yukishige Ito, Naoshi Dohmae, Kiyotaka Fujita, Akihiro Ishiwata, Shinya Fushinobu
Summary: This study investigated the catalytic mechanism and substrate binding structure of the beta-l-arabinofuranosidase HypBA1 from Bifidobacterium longum. Zn2+ ion and cysteine were found to play key roles in catalysis, and the mode of substrate binding affected the catalytic activity.
Article
Biochemistry & Molecular Biology
Jagat K. Chhipi-Shrestha, Tilman Schneider-Poetsch, Takehiro Suzuki, Mari Mito, Khalid Khan, Naoshi Dohmae, Shintaro Iwasaki, Minoru Yoshida
Summary: Splicing modulators induce the translation of intron-retained mRNAs, generating truncated proteins. These truncated proteins have intrinsically disordered regions, form insoluble cellular condensates, and trigger the proteotoxic stress response, thereby inhibiting protein synthesis in cells.
CELL CHEMICAL BIOLOGY
(2022)
Article
Biochemical Research Methods
Saranya Krishnan, Shina Sasi, Preshobha Kodakkattumannil, Salima Al Senaani, Geetha Lekshmi, Martin Kottackal, Khaled M. A. Amiri
Summary: This study aimed to develop an efficient DNA extraction protocol suitable for diverse plant species and tissues. A reliable and consistent protocol was described for the extraction of high-quality DNA from difficult-to-extract plant species. The optimized protocol was successful in extracting high-quality DNA from various plant species and tissues, making it useful for genomic studies of recalcitrant plants.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Eisaku Hokazono, Saori Fukumoto, Takeshi Uchiumi, Susumu Osawa
Summary: This study proposes a method for detecting nucleic acid amplification using pyrophosphate, which requires only two reagents and an automated analyzer. The technique has high sensitivity and reproducibility, and can detect pyrophosphate within 10 minutes. Therefore, this method has the potential to be a new, rapid, and simple detection technique for amplified nucleic acids.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Drew S. Sowersby, L. Kevin Lewis
Summary: SURE electrophoresis is a new method for concentrating samples in gels, which allows efficient detection of highly dilute DNA samples. This approach generates single bands with enhanced signal intensities and minimal band broadening.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Hongyi Liang, Guoliang Yin, Guangxi Shi, Zhiyong Liu, Xiaofei Liu, Jingwei Li
Summary: The mechanism of Echinacoside (ECH) in treating breast cancer (BC) was explored through network pharmacology and experimental validation. It was found that ECH plays an important role in anti-BC by regulating the PI3K/AKT/HIF-1 alpha/VEGF signaling pathway, and it exhibits multi-target and multi-pathway effects.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Daniel S. Kantner, Emily Megill, Anna Bostwick, Vicky Yang, Carmen Bekeova, Alexandria Van Scoyk, Erin L. Seifert, Michael W. Deininger, Nathaniel W. Snyder
Summary: This study compared the results of different methods for measuring the amount of acetyl-Coenzyme A. The colorimetric ELISA kit did not produce interpretable results, while the fluorometric enzymatic kit showed comparable results to the LC-MS-based methods depending on the matrix and extraction conditions. LC-MS/MS and LC-HRMS methods produced well-aligned results, especially when using stable isotope-labeled internal standards.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Jingyan Xu, Hanying Tan, Xionghui Ma, Linjing Su, Zhi Zhang, Yuhao Xiong
Summary: This study investigates the co-catalytic capabilities of MoO3 nanosheets in enhancing the enzyme-like catalytic activity of a two-dimensional ultrathin Fe(III)-modified covalent triazine framework (Fe-CTF) under neutral pH conditions. The Fe-CTF/MoO3 co-catalytic system exhibits enzyme-mimicking activity and enables the development of a colorimetric method for glucose detection. Furthermore, a straightforward one-pot colorimetric method is established for screening XOD inhibitors.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Lichao Zhang, Kang Xiao, Xueting Wang, Liang Kong
Summary: A novel fusion technology was designed to identify FAD-binding sites, achieving the best results on two independent test datasets and outperforming existing methods significantly. The high performance and certainty of the method were demonstrated through statistical tests and cross-entropy loss analysis.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Abdulkadir Kilic, Mehmet Aslan, Abdulkadir Levent
Summary: This study developed a simple, rapid, sensitive, and selective voltammetric technique for the electrochemical characterization and detection of the highly risky drug Edoxaban. The optimized voltammetric method showed good analytical working range and was successfully applied to urine and tablet samples.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Su Hyun Kim, Nara Shin, Jong-Min Jeon, Jeong-Jun Yoon, Jeong Chan Joo, Hee Taek Kim, Shashi Kant Bhatia, Yung-Hun Yang
Summary: To address environmental issues caused by traditional plastics, bioplastics have gained attention as alternatives. Although bioplastics have better degradability, their degradation still takes longer than anticipated. This study proposes a novel screening method to identify bioplastic degraders faster, saving time and providing more quantitative data.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Irem Okman Kocoglu, Pinar Esra Erden, Esma Kilic
Summary: In this study, an electrochemical biosensor based on carbon nanofibers and ionic liquid modification was constructed for tyramine detection. The biosensor showed linear response, low detection limit, high sensitivity, and exhibited good reproducibility, stability, and anti-interference ability.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Liana U. Akhmetzianova, Timur M. Davletkulov, Assol R. Sakhabutdinova, Alexey Chemeris, Irek M. Gubaydullin, Ravil R. Garafutdinov
Summary: A new program called LAMPrimers iQ has been developed for high-quality LAMP primer design, and its advantages in providing higher specificity and reliable detection of viral RNA were validated using SARS-CoV-2 coronavirus RNA as a model target.
ANALYTICAL BIOCHEMISTRY
(2024)
Article
Biochemical Research Methods
Tommaso Pileri, Alberto Sinibaldi, Agostino Occhicone, Norbert Danz, Elena Giordani, Matteo Allegretti, Frank Sonntag, Peter Munzert, Patrizio Giacomini, Francesco Michelotti
Summary: This study developed a biosensing device based on one-dimensional photonic crystal to detect HER2 in breast cancer. The device combines label-free and fluorescence operation modes, allowing for real-time and accurate detection in less than 20 minutes. It offers a promising technique for combined label-free and fluorescence detection in disease diagnosis and therapeutic monitoring.
ANALYTICAL BIOCHEMISTRY
(2024)
