期刊
ANALYTICA CHIMICA ACTA
卷 1071, 期 -, 页码 70-77出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2019.04.049
关键词
Aptamer; Pb2+ detection; Graphene oxide; Paper-based aptasensor; G-quadruplex; Fluorescence microscopy
资金
- Research Council of Ferdowsi University of Mashhad [3/44446]
- Photographic Center of Biology Department of Ferdowsi University of Mashhad [BX51]
In this study, a simple paper-based aptasensor has been developed for the ultrasensitive detection of lead (Pb2+) ion within about 10 min. The aptasensor has been successfully designed by taking advantages of the Forster Resonance Energy Transfer (FRET) process and the super fluorescence quenching property of graphene oxide (GO) sheet. The sensing mechanism of the aptasensor is based on the conformational switch of the Pb2+-specific aptamer from a random coil to a G-quadruplex structure. An injection of Pb2+ on the paper-based platform induces the release of the specific aptamer from the GO surface that recovers the fluorescence emission. Under the optimal experimental conditions, there is a good linear relationship between the fluorescence recovery and the Pb2+ concentration in the ranges of 5-70 pM and 0.07-20 nM. Moreover, the aptasensing array exhibits a high sensitivity to Pb2+ with an ultra-low detection limit of 0.5 pM. The developed aptasensor has been successfully applied to determine Pb2+ in tap water, lake water, milk, and human blood serum. The paper-based aptasensor can be efficiently utilized to detect other metal ions and biological molecules by substituting target specific aptamer. (C) 2019 Elsevier B.V. All rights reserved.
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