Article
Medicine, Research & Experimental
Parisa Ghaffari-Makhmalbaf, Maryam Sayyad, Katayoon Pakravan, Ehsan Razmara, Amirreza Bitaraf, Babak Bakhshinejad, Parmida Goudarzi, Hassan Yousefi, Mahmoud Pournaghshband, Fahimeh Nemati, Hossein Fahimi, Fatemeh Rohollah, Mandana Hasanzad, Mehrdad Hashemi, Seyed Hadi Mousavi, Sadegh Babashah
Summary: The research demonstrates that DHA treatment can inhibit angiogenesis of breast cancer cells by altering the exosomal miRNA contents from pro-angiogenic to anti-angiogenic properties.
Review
Cardiac & Cardiovascular Systems
Amankeldi A. Salybekov, Aidyn D. Kunikeyev, Shuzo Kobayashi, Takayuki Asahara
Summary: Extracellular vesicles (EVs) secreted by nucleated cells, especially endothelial progenitor cell-derived EVs (EPC-EVs), have shown beneficial effects in various diseases such as cardiovascular and lung disorders, accumulating at injury sites with angiogenic and restorative effects surpassing their ancestors. This review article focuses on the biogenesis, delivery routes, and long-term storage methods of EPC-EVs, highlighting their anti-inflammatory, angiogenic, and tissue protection effects in diseases, and discussing the therapeutic potential of EPC-EVs in clinical applications.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Engineering, Biomedical
Zhiming Shen, Fei Sun, Yibo Shan, Yi Lu, Cong Wu, Boyou Zhang, Qiang Wu, Lei Yuan, Jianwei Zhu, Qi Wang, Yilun Wang, Wenxuan Chen, Yaojing Zhang, Wenlong Yang, Yiwei Fan, Hongcan Shi
Summary: A novel cell-free tissue-engineered tracheal scaffold was developed to promote vascularization using endothelial progenitor cell exosomes. The results demonstrated the excellent angiogenic potential of the scaffold and its capacity to repair tracheal defects.
MATERIALS TODAY BIO
(2023)
Article
Cell & Tissue Engineering
Linlin Guo, Yuxuan Chen, Xiaoling Feng, Di Sun, Jiaming Sun, Shan Mou, Kangcheng Zhao, Ran An
Summary: This study reveals that exosomes derived from HUVECs exposed to oxidative stress significantly enhance the pro-angiogenic ability of EPCs through the Lnc NEAT1-mediated Wnt/β-catenin signaling pathway, thereby improving skin flap survival. This finding provides a potential alternative therapy for improving the survival of skin flaps.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Jieun Lee, Hal Sternberg, Paola A. Bignone, James Murai, Nafees N. Malik, Michael D. West, Dana Larocca
Summary: This study presents a panel of clonal embryonic endothelial progenitor cell lines derived from human embryonic stem cells. These cell lines exhibit characteristics of endothelial cells and show strong transcriptomic similarity to adult endothelial primary cell lines. The cells have demonstrated scalability and stable angiogenic properties, making them a potential source for therapeutic cells in cardiovascular disease treatment.
Article
Cell Biology
Yi-Nan Lee, Hsueh-Hsiao Wang, Cheng-Huang Su, Hsin- Lee, Yen-Hung Chou, Chin-Ling Hsieh, Wen-Ting Liu, Kuo-Tung Shu, Kai-Ting Chang, Hung- Yeh, Yih-Jer Wu
Summary: The study established an accelerated senescence model by treating endothelial progenitor cells (EPCs) with the iron chelator deferoxamine (DFO). Results showed that DFO treatment induced senescent phenotypes in EPCs and could validate its effects in vivo.
Article
Chemistry, Multidisciplinary
Bing Dong, Yumin Qiu, Zhefu Liu, Jinsheng Huang, Zhichao Wang, Qiang Tu, Zhe Zhou, Jiang He, Yong Wang, Xiaolin Liu, Jianning Zhang, Xintao Shuai, Jun Tao, Wenhao Xia
Summary: By studying EPC-EXO from patients with coronary heart disease and healthy volunteers, it was found that C-EPC-EXO was less effective than H-EPC-EXO in improving angiogenesis. Manipulating the bioactivities of C-EPC-EXO through miR-199a-3p inhibition and cRGD decoration can significantly promote ECs-mediated angiogenesis for treating ischemic vascular diseases.
Article
Immunology
Glenn Fitzpatrick, Danielle Nader, Rebecca Watkin, Claire E. E. McCoy, Gerard F. F. Curley, Steven W. W. Kerrigan
Summary: This study demonstrates that endothelial-derived exosomes drive a pro-inflammatory phenotype in monocytes through dysregulated expression of miR-99a and miR-99b.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Neurosciences
Qunwen Pan, Yan Wang, Jinhua Liu, Xiaojuan Jin, Zhi Xiang, Suqing Li, Yumeng Shi, Yanfang Chen, Wangtao Zhong, Xiaotang Ma
Summary: Aging is a key factor in vascular dysfunction and the development of ischemic stroke. ACE2-enriched exosomes derived from endothelial progenitor cells (EPCs) have been shown to protect aging endothelial cells (ECs) from hypoxia-induced injury. This study aimed to investigate the potential of ACE2-enriched EPC exosomes (ACE2-EPC-EXs) in reducing brain ischemic injury by inhibiting cerebral EC damage through miR-17-5p and associated molecular mechanisms. The results demonstrated that ACE2-EPC-EXs were more effective in improving cerebral microvascular density, blood flow, and reducing brain cell senescence, infarct volume, and neurological deficit in aged mice compared to EPC-EXs. In aging ECs, ACE2-EPC-EXs also showed greater efficacy in reducing senescence, oxidative stress, and apoptosis, and promoting cell viability and tube formation compared to EPC-EXs. This research suggests that ACE2-EPC-EXs have promising protective effects against neurovascular injury in aging ischemic stroke by activating the miR-17-5p/PTEN/PI3K/Akt signaling pathway.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Medicine, General & Internal
Peng Li, Guanhao Hong, Weiqiang Zhan, Mingzhu Deng, Chenlin Tu, Jinsong Wei, Hao Lin
Summary: This study investigated the effects and mechanisms of EPCs-EXO on diabetic wound healing. The results showed that EPCs-EXO promoted proliferation and migration, while inhibiting apoptosis of skin cells in a high glucose environment. Additionally, EPCs-EXO inhibited the expression of PPARG, leading to enhanced wound healing in diabetic mice.
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Jia Xia, Xiaoying Song, Jing Meng, Danfei Lou
Summary: EPCs-Exos inhibit Erastin-induced HUVEC ferroptosis by upregulating miR-30e-5p, inhibiting SP1, and activating the AMPK pathway.
Article
Immunology
Xiaoyu Pan, Lin Yang, Shuqi Wang, Yanhui Liu, Lin Yue, Shuchun Chen
Summary: The low-grade inflammatory state in obesity can damage vascular endothelial cells and lead to cardiovascular diseases. Macrophage exosomes improve glucose tolerance and insulin sensitivity in obese mice, but the relationship with endothelial cell injury is unclear. This study examined the effects of lipopolysaccharide (LPS)-induced macrophage exosomes on endothelial progenitor cells (EPCs) and inflammatory factors. It also investigated the role of MicroRNA-155 (miR-155) in macrophage exosomes and EPCs, and the impact of semaglutide on EPC function and inflammation. The results showed that LPS-induced macrophage exosomes impaired EPCs function and increased inflammation, which was exacerbated by high miR-155 expression. In contrast, inhibition of miR-155 improved EPCs function and reduced inflammation. Semaglutide enhanced EPCs viability and reduced inflammatory factors and miR-155 expression in exosomes.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xiuya Li, Yilin Lian, Yukang Wu, Zihui Ye, Jiabao Feng, Yuan Zhao, Xudong Guo, Jiuhong Kang
Summary: In this study, it was found that intramyocardial delivery of plasma exosomes from neonatal mice could help repair the adult heart after acute myocardial infarction (AMI). Cardiac endothelial cells received the majority of exosomal ligands and the pro-angiogenic effect of exosomes was mediated through the recognition of cardiac endothelial cell receptors. This ligand-receptor network provides inspiration for cardiac regeneration post-MI.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Cheng Peng, Li-jun Yang, Chuan Zhang, Yu Jiang, Liu-wen-xin Shang, Jia-bei He, Zhen-wei Zhou, Xia Tao, Lu Tie, Alex F. Chen, He-hui Xie
Summary: Low-dose nifedipine has been found to enhance the angiogenic potential of endothelial progenitor cells (EPCs) and protect against cerebral ischemic injury in diabetic mice. The treatment with nifedipine significantly increased the number of circulating EPCs, improved EPC function, and promoted angiogenesis. It also reduced the levels of inhibitory factors in EPCs. This study suggests that long-term treatment with low-dose nifedipine can be a safe and cost-effective strategy to prevent ischemic diseases in diabetes.
ACTA PHARMACOLOGICA SINICA
(2023)
Review
Oncology
Claudia Palazzo, Alessio D'Alessio, Luca Tamagnone
Summary: Extracellular vesicles (EVs) play an important role in the communication mechanisms between cancer and endothelial cells, and may have potential implications in the treatment of vascular pathologies. EVs are able to transport a variety of cell constituents and regulate the functions of endothelial cells. Cancer cell-derived EVs have been found to have a controlling effect on endothelial cell functions and are involved in tumor angiogenesis and cancer progression.