Article
Chemistry, Multidisciplinary
Juanzu Liu, Hongmin Meng, Lin Zhang, Shasha Li, Juan Chen, Yi Zhang, Jianjun Li, Lingbo Qu, Zhaohui Li
Summary: A simple dual-readout lateral flow test strip platform was developed for sensitive detection of alkaline phosphatase (ALP) based on a portable device. The method utilizes colorimetric and fluorescent signals to quantitatively detect trace amounts of ALP, showing great potential for clinical diagnosis.
CHINESE CHEMICAL LETTERS
(2021)
Article
Chemistry, Applied
Tingting Liu, Xiaocui Lai, Ping Guo, Wei Zhang, Gan Zhang, Mengyun Wu, Guangjian Xue, Xuechen Fang, Juan Peng, Weihua Lai
Summary: To protect consumers' health, a rapid and sensitive method for the detection of kanamycin (KAN) residue in animal products needs to be established. In this study, a KAN monoclonal antibody (KAN-mAb) with a half maximal inhibitory concentration of 1.16 ng/mL was prepared. Fe3+-chelated polydopamine nanospheres (Fe@PDANs) were synthesized using a one-pot method and used as a novel label in the development of a lateral flow immunoassay (LFIA) for KAN detection. The Fe@PDANs-based LFIA (Fe@PDANs-LFIA) showed a lower limit of detection for KAN compared to the PDANs-based LFIA and successfully detected KAN in pork, milk, and honey samples with high recoveries.
Article
Chemistry, Multidisciplinary
Hyung-Mo Kim, Chiwoo Oh, Jaehyun An, Seungki Baek, Sungje Bock, Jaehi Kim, Heung-Su Jung, Hobeom Song, Jung-Won Kim, Ahla Jo, Dong-Eun Kim, Won-Yeop Rho, Jin-Young Jang, Gi Jeong Cheon, Hyung-Jun Im, Bong-Hyun Jun
Summary: A new technique utilizing highly bright multi-quantum dots embedded nanoparticles was introduced to improve exosome detection. This method achieved highly sensitive and quantitative detection of exosomes through lateral flow immunoassay.
Article
Chemistry, Analytical
Tianhao Li, Zhiwei Deng, Jiaqi Bu, Hui Liu, Yanjing Yang, Shian Zhong
Summary: A test strip for selective detection of ferritin was developed by integrating molecular imprinting technology and multicolor fluorescence elements. The test strip showed high specificity for ferritin and allowed for rapid and visual detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Vera A. Bragina, Elena Khomyakova, Alexey V. Orlov, Sergey L. Znoyko, Elizaveta N. Mochalova, Liliia Paniushkina, Victoria O. Shender, Thalia Erbes, Evgeniy G. Evtushenko, Dmitry V. Bagrov, Victoria N. Lavrenova, Irina Nazarenko, Petr I. Nikitin
Summary: This research developed a highly sensitive and easy-to-use immunochromatographic tool for the quantification of extracellular vesicles (EVs). The tool demonstrates advantages in detection limit, specificity, and cost-effectiveness, and can be used for liquid biopsy in daily clinical routines and extended to other relevant biomarkers.
Article
Chemistry, Applied
Harpreet Singh, Shalini Singh, Sanjeev K. Bhardwaj, Gurjeet Kaur, Madhu Khatri, Akash Deep, Neha Bhardwaj
Summary: In this research, a novel fluorescent immunosensor based on nitrogen-doped carbon quantum dots has been developed for the analysis of aflatoxin M1 in dairy products. The sensor exhibited high sensitivity and low limit of detection, and was further developed as a lateral flow design for on-site detection.
Article
Biochemical Research Methods
Haicong Shen, Eunyeong Song, Yang Wang, Lingyan Meng, Jing Dong, Bingqian Lin, Di Huang, Zhichao Guan, Chaoyong Yang, Zhi Zhu
Summary: The lateral flow assay (LFA) as a paper-based analytical platform has the advantages of rapid analysis, low cost, and user-friendliness, but suffers from low detection sensitivity via the naked eye. Various nanoparticle signal tags have been employed to enhance the signal readout, with Raman reporter-labeled nanoparticles showing potential for sensitive detection. The application of LFA has been improved with the development of an in situ Raman enhancement strategy, resulting in highly sensitive and quantitative detection suitable for rapid diagnosis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Jianyu Zhu, Leina Dou, Jiafei Mi, Yuchen Bai, Minggang Liu, Jianzhong Shen, Wenbo Yu, Suxia Zhang, Xuezhi Yu, Zhanhui Wang
Summary: Phallotoxins are toxic cyclopeptides found in wild poisonous mushrooms, and a highly sensitive monoclonal antibody (mAb) was produced for early and rapid diagnosis of mushroom toxin poisoning. Two lateral flow assays (LFA) based on colloidal gold nanoparticle (GNP) and time-resolved fluorescent nanosphere (TRFN) were developed for qualitative screening and quantitative determination of the severity of poisoning, achieving fast analysis within 10 minutes.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Tong Lin, Xiaolin Huang, Liang Guo, Shu Zhou, Xiangmin Li, Yuanjie Liu, Jing Hu, Xirui Chen, Yonghua Xiong
Summary: The study presents a novel method for preparing immunoprobes that improves antibody orientation and biological activity, resulting in significant enhancement in lateral flow immunoassay performance.
MICROCHEMICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Qian Han, Ling Fan, Xiuying Liu, Yiwei Tang, Pingping Wang, Zaixi Shu, Wei Zhang, Lijie Zhu, Kevin Critchley
Summary: In this study, quantum dot nanobeads were used as a signal source to develop a competitive lateral flow immunoassay for the detection of chloramphenicol. The developed method showed high sensitivity and accuracy, allowing for quantitative detection of CAP in a wide range.
Article
Biochemical Research Methods
Iida Martiskainen, Sheikh M. Talha, Karoliina Vuorenpaa, Teppo Salminen, Etvi Juntunen, Souvick Chattopadhyay, Dinesh Kumar, Tytti Vuorinen, Kim Pettersson, Navin Khanna, Gaurav Batra
Summary: A highly sensitive rapid diagnostic test for hepatitis B Virus surface antigen (HBsAg) was developed in this study, which can meet the needs for patients with low levels of HBsAg and blood screening. UCNP-LFIA showed higher sensitivity and accuracy in sample detection, making it a reliable detection method.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Food Science & Technology
Shibei Shao, Wenhua Shang, Yuchen Bai, Leina Dou, Suxia Zhang, Jianzhong Shen, Zhanhui Wang, Kai Wen
Summary: In this study, a highly specific and high affinity monoclonal antibody 8A9 was prepared for the detection of diacetoxyscirpenol (DAS), a type A trichothecene, in rice samples. An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a lateral flow immunoassay (LFIA) based on the monoclonal antibody were developed, with detection limits as low as 0.65 μg/kg and 100 μg/kg, respectively. Molecular docking analysis revealed the molecular recognition mechanism of the antibody, demonstrating the highly specific recognition of DAS through hydrogen bonds and a pi-sigma bond.
Article
Engineering, Electrical & Electronic
Yuan Liu, Cong Zhao, Jingzhou Li, Shixi Zhao, Xiaomin Xu, H. Y. Fu, Cunjiang Yu, Feiyu Kang, Guodan Wei
Summary: The high-performance photodetectors based on environmentally benign CuInS2/ZnS core-shell quantum dots, in combination with organic blend films, achieve efficient energy transfer and charge separation, leading to low dark current and high detectivity. This work demonstrates a feasible strategy to develop highly sensitive photodetectors using heavy-metal-free core-shell quantum dots.
ACS APPLIED ELECTRONIC MATERIALS
(2021)
Article
Chemistry, Analytical
Meng Dang, Zizhe Li, Yexuan Mao, Xianqing Huang, Lianjun Song, Wenfeng Li, Ruxiang Ma, Yang Liu, Liye Wang, Xuezhi Yu, Huijuan Yang, Xiya Zhang
Summary: A new highly sensitive lateral flow immunoassay (LFA) based on amorphous carbon nanoparticles (ACNs) was developed for high-throughput screening of sulfonamides (SAs) residues in milk samples, especially sulfamethazine (SM2). This LFA utilized a group-specific monoclonal antibody 10H7 (mAb 10H7) conjugated to ACNs as an immune probe, achieving a sensitive detection with a cut-off value of 2 ng/mL for 25 SAs. The LFA was successfully applied for screening SAs residues in real milk samples, showing consistent results with HPLC-MS/MS.
Article
Biophysics
Sunghwan Kim, Yujin Ahn, Yoonji Bae, Sunyoung Woo, Jungsik Park, Im Kyung Han, Hyunhong Kim, Soomin Eom, Sebyung Kang, Woonggyu Jung, Jongnam Park
Summary: In this study, a highly sensitive pregnancy test kit was developed based on brush-type ligand-coated quantum beads and nanobody, which enhanced the detection capacity of antibodies. A smartphone-based reader was also developed for convenient acquisition of the test results.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Nanoscience & Nanotechnology
Yanmei Shen, Lin Zhang, Demiao Ma, Qianqian Zhu, Hongmin Meng, Lingbo Qu, Zhaohui Li, Jia Ge
Summary: In this study, a fluorimetric assay for ultrasensitive determination of microRNA-141 was designed based on WS2 quantum dots and concatenated DNA circuit-assembled DNAzymes. The developed biosensor shows great potential for the analysis of biomarkers and early diagnosis of microRNA-related diseases.
ACS APPLIED NANO MATERIALS
(2022)
Article
Chemistry, Analytical
Qiannan Li, Yufei Wang, Qianqian Zhu, Haifang Liu, Jianbo Liu, Hong-Min Meng, Zhaohui Li
Summary: A novel dual-mode sensing system based on molybdophosphoric heteropoly acid (PMA) and fluorescent microspheres (FMs) was developed for monitoring alkaline phosphatase (ALP) activity. The system utilized the catalytic hydrolysis of l-ascorbic acid-2-phosphate (AAP) by ALP to generate a colorimetric signal and reduce PMA to phosphorus molybdenum blue (PMB). Additionally, the fluorescence of FMs was quenched through the inner-filter effect, enabling simultaneous fluorescence detection. The system showed high sensitivity with a limit of detection of 0.27 U L-1 and 0.11 U L-1, and demonstrated good analytical performance in biological samples. A portable test kit for visual detection of ALP in serum samples was also developed using a smartphone as a visual detection platform.
Article
Chemistry, Analytical
Xing Wang, Hongzhi Sun, Bojun Liu, Kemei Jiang, Zhaohui Li, Hong-Min Meng
Summary: The gold nanoparticle-based three-dimensional DNA walking nanomachine shows promise in molecular diagnostics and cancer therapy, but the disordered assembly between the walker and the track limits its executive ability. In this study, a well-directed 3D DNA walking nanomachine was developed using a DNA dendrimer as the track, allowing for high directionality and controllability. The distribution of the substrate strand and DNAzyme on the DNA dendrimer could be precisely regulated, providing a clear route for each walker. This nanomachine has stability and anti-interference ability, making it useful for molecular diagnostics and biological function modulation.
ANALYTICAL CHEMISTRY
(2022)
Article
Engineering, Environmental
Jie Zhang, Yingying Li, Jingyu Hu, Yuanqiang Sun, Ran Yang, Zhaohui Li, Lingbo Qu
Summary: This study developed a rapid, visual, and on-site detection method for phosgene using sensitive and selective carbon dots as detection materials. The detection of phosgene was achieved by modifying the color and fluorescence of the carbon dots. Additionally, a portable droplet system was developed for on-site detection and long-term visualization of phosgene.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Analytical
Zengqiang Liang, Yuanqiang Sun, Huajin Zeng, Haimei Qin, Ran Yang, Lingbo Qu, Ke Zhang, Zhaohui Li
Summary: Sensing systems based on cholinesterase and carboxylesterase combined with various transduction technologies have been widely used for pesticide screening due to their simplicity, speed, and suitability for on-site analysis. However, current enzymatic sensors face a challenge in the broad-spectrum and specific screening of pyrethroids. Human serum albumin (HSA), a multifunctional protein, has been found to have a catalytic activity in hydrolyzing the coumarin lactone bond, which enables the development of a specific and broad-spectrum pyrethroids sensing system.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Inorganic & Nuclear
Guohai Yang, Fanglei Liu, Jiayi Zhao, Lijie Fu, Yingqiu Gu, Lulu Qu, Chengzhou Zhu, Jun-Jie Zhu, Yuehe Lin
Summary: In this review, the latest research on MXenes in the fields of biosensing and biomedicine is summarized. The synthesis strategies and unique properties of MXenes are introduced, and the biosensing applications of MXenes are divided into three subcategories based on the sensing mechanism: electrochemical, photochemical, and opto-electrochemical biosensors. The biomedical applications of MXenes are evaluated from four aspects: antibacterial activity, bioimaging, therapeutics, and theranostics. The specific chemical and physical properties of MXenes that influence the efficiency of biosensing and biomedicine are highlighted, as well as the structure-performance relationships determining detection sensitivity and material toxicity. The challenges, future prospects, and opportunities for MXenes are discussed.
COORDINATION CHEMISTRY REVIEWS
(2023)
Article
Chemistry, Physical
Xiao-Qian Zhang, Zhaoyuan Lyu, Shichao Ding, Shiyong Zhao, Guangyu Chen, Yuehe Lin, Jin-Cheng Li
Summary: A molten-salt-assisted pyrolysis strategy was used to prepare two-dimensional porous metal-nitrogen/carbon materials with exposed metal-nitrogen sites, which showed excellent performance in both liquid-state and solid-state Zn-air fuel cells.
JOURNAL OF PHYSICAL CHEMISTRY C
(2023)
Article
Chemistry, Analytical
Yaqing Yang, Simin Wei, Jialin Wang, Junjie Li, Jinlu Tang, Albert Aryee Aaron, Qiyong Cai, Ningning Wang, Zhaohui Li
Summary: In this study, a NO2--activated ratiometric upconversion luminescence (UCL) nanosensor was designed for NO2- detection. The nanosensor utilized the inner filter effect (IFE) between NO2--sensitive carbon dots (CDs) and upconversion nanoparticles (UCNPs) to achieve detection. By taking advantage of NIR excitation and ratiometric detection signal, the nanosensor effectively eliminated autofluorescence and successfully detected NO2- quantitatively in actual samples. This nanosensor provides a simple and sensitive sensing strategy for NO2- detection and analysis, which can be utilized in food safety.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Qiyong Cai, Shuiling Jin, Hong Zong, Lu Pei, Ke Cao, Lingbo Qu, Zhaohui Li
Summary: This study developed a quadruplex ultrasensitive immunoassay for simultaneous assessment of four reproductive hormone proteins in various biofluid samples. The assay exhibited good performance and consistency with clinical test results, allowing for rapid and accurate diagnosis of reproductive endocrine disorders.
ANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Environmental
Junli Wang, Yuanqiang Sun, Jingyu Hu, Yifei Guo, Lingbo Qu, Zhaohui Li
Summary: This study developed a novel near-infrared carbon dots with dual-report function that accurately identifies the polarity and spatial characteristics of lipid droplets in hepatocellular carcinoma (HCC). The carbon dots can report the number, size, aggregation degree, and polarity variations of lipid droplets using fluorescence imaging technology, enabling high-contrast fluorescence diagnosis of HCC and evaluation of anticancer drug efficacy.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Spectroscopy
Sen Li, Jingyu Hu, Aaron Albert Aryee, Yuanqiang Sun, Zhaohui Li
Summary: Disposable medical masks are widely used to prevent respiratory infections, but their improper disposal poses a threat to the environment and human health. This study proposes a hydrothermal method to disinfect and transform waste medical masks into carbon dots with blue-emissive fluorescence, providing a sustainable and value-added solution.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Multidisciplinary
Juan Liu, Xiaoxiao Guo, Zhaoyuan Lyu, Rong-Bin Song, Pengyu Zhou, Shichao Ding, Yang Zhou, Li-Ping Jiang, Yuehe Lin, Wenlei Zhu
Summary: By using copper electrocatalysts and microorganisms, an electro-bio tandem reactor was designed to convert CO2 into specific multicarbon compounds in both the gas and liquid phases. The reducing products from electrocatalysis can be consumed and recycled for selective acetate synthesis in Moorella thermoacetica. The results showed high faradaic selectivity for acetate in liquid products for Cu-based CO2 electroreduction and high electron conversion rate to ethylene and acetate for microbial electrosynthesis, presenting a green design for CO2 upcycling targeting multicarbon products through the parallel integration of electrocatalysis and fermentation.
Review
Chemistry, Multidisciplinary
Weiqing Xu, Yu Wu, Wenling Gu, Dan Du, Yuehe Lin, Chengzhou Zhu
Summary: Metal-organic frameworks (MOFs) have made significant advances as mimics of metalloenzymes in bioinspired catalysis, particularly in the field of active pocket engineering at the atomic level.
CHEMICAL SOCIETY REVIEWS
(2023)
Article
Chemistry, Multidisciplinary
Shuo Feng, Rajesh Kumar Singh, Yucheng Fu, Zhuo Li, Yulong Wang, Jie Bao, Zhijie Xu, Guosheng Li, Cassidy Anderson, Lili Shi, Yuehe Lin, Peter G. Khalifah, Wei Wang, Jun Liu, Jie Xiao, Dongping Lu
Summary: In this study, a simple strategy was demonstrated for constructing low-tortuosity through-pores in both vertical and planar directions of electrodes by casting large particles into single-particle-layer electrodes. Through multi-scale characterizations and simulations, correlations between material/electrode structures, electrolyte permeability, polysulfide migration, and sulfur reactions were elucidated. The high-loading and dense sulfur cathode fabricated by this method delivers a high specific capacity at a very low electrolyte/sulfur ratio.
ENERGY & ENVIRONMENTAL SCIENCE
(2022)
Review
Chemistry, Multidisciplinary
Yu Wu, Weiqing Xu, Lei Jiao, Wenling Gu, Dan Du, Liuyong Hu, Yuehe Lin, Chengzhou Zhu
Summary: Enzymes have great potential in various fields due to their high activity and specificity for catalysis under mild conditions. However, they struggle to adapt to different environments outside of their familiar biological conditions. Nanobiocatalysis, which integrates nanotechnology and enzymatic catalysis, aims to design biocatalysis based on materials science. Nano-integrated biocatalysis and bio-inspired nanocatalysis are two effective nanobiocatalytic systems that can meet different design needs, and their combination opens up even more possibilities. This review summarizes the types, constructions, and functions of nanobiocatalytic systems, analyzing the pros and cons of different strategies. Additionally, it highlights their applications in bioassay, biotherapy, and environmental remediation.
CHEMICAL SOCIETY REVIEWS
(2022)