期刊
BIOMOLECULES
卷 9, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/biom9060249
关键词
Ranatuerin; antimicrobial peptides; alpha-helix; membrane permeability; waxworm model
资金
- Science and Technology Planning Project of Guangdong Province [2015A010107018]
- Shaoguan Science and Technology Planning Project [2018sn157]
- Shaoguan Social Development Science and Technology Plan Project [2018Sn080]
- Science and Technology Development Fund of Macau SAR (FDCT) [019/2017/A1]
- Faculty of Health Sciences (FHS) University of Macau (UM)
- FHS UM
Antimicrobial peptides (AMPs) are considered as a promising agent to overcome the drug-resistance of bacteria. Large numbers of AMPs have been identified from the skin secretion of Rana pipiens, including brevinins, ranatuerins, temporins and esculentins. In this study, the cDNA precursor of a broad-spectrum antimicrobial peptide, ranatuerin-2Pb, was cloned and identified. Additionally, two truncated analogues, RPa and RPb, were synthesised to investigate the structure-activity relationship of ranatuerin-2Pb. RPa lost antimicrobial activity against Candida albicans, MRSA, Enterococcus faecalis and Pseudomonas aeruginosa, while RPb retained its broad-spectrum antimicrobial activity. Additionally, ranatuerin-2Pb, RPa and RPb demonstrated inhibition and eradication effects against Staphylococcus aureus biofilm. RPb showed a rapid bacterial killing manner via membrane permeabilization without damaging the cell membrane of erythrocytes. Moreover, RPb decreased the mortality of S. aureus infected Galleria mellonella larvae. Collectively, our results suggested that RPb may pave a novel way for natural antimicrobial drug design.
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