期刊
BIOORGANIC & MEDICINAL CHEMISTRY
卷 23, 期 21, 页码 6855-6868出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmc.2015.09.048
关键词
Wnt signaling pathway; Porcupine; Antagonist; Scaffold hybridization; Cancer therapy
资金
- National Natural Science Foundation of China [81273372, 81473090, 81473278, 21502133]
- Natural Science Foundation of Jiangsu Province [BK2012004, BK20140313, BM2013003]
- PAPD - Priority Academic Program Development of Jiangsu Higher Education Institutions
The Wnt signaling pathway is a pivotal developmental pathway. It operates through control of cellular functions such as proliferation, differentiation, migration and polarity. Aberrant Wnt signaling has been implicated in the formation and metastasis of tumors. Porcupine is a component of the Wnt signaling pathway. It is a member of the membrane-bound O-acyltransferase family of proteins. Porcupine catalyzes the palmitoylation of Wnt proteins, a process which is essential to their secretion and activity. Here we report a novel series of compounds obtained by a scaffold hybridization strategy from two known porcupine inhibitor classes. The leading compound 62 demonstrated subnanomolar (IC50 0.11 nM) inhibition of Wnt signaling in a paracrine cellular reporter gene assay. Compound 62 also potently inhibited Wnt secretion into culture medium, an indication of direct inhibition of the porcupine protein. Furthermore, compound 62 showed excellent chemical, plasma and liver microsomal stabilities. Collectively, these results strongly support further optimization of this novel scaffold to develop better Wnt pathway inhibitors. (C) 2015 Elsevier Ltd. All rights reserved.
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