Article
Immunology
Jiang Jiang, Wenfang Wang, Lan Zhu, Bowen Shi, Yong Chen, Yihan Xia, Weiming Feng, Weiwu Yao, Aiguo Lu, Huan Zhang
Summary: The crosstalk between tumor cells and macrophages under hypoxic conditions, facilitated by exosomes, plays a crucial role in promoting colorectal cancer (CRC) progression. This study explores the impact of hypoxic macrophage-derived exosomes (HMDEs) on CRC progression and identifies the underlying molecular mechanisms. It is found that HMDEs induce cell cycle transition and inhibit cell apoptosis, promoting CRC cell growth. The interaction between Hif-1 alpha and Hsp90, as well as Hsp90 and Lats1, is identified as the key molecular mechanism. These findings provide valuable insights into the complex interplay between macrophages and CRC cells in hypoxic conditions.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Physiology
Geoffrey Warnier, Estelle De Groote, Florian A. Britto, Ophelie Delcorte, Joshua P. Nederveen, Mats I. Nilsson, Christophe E. Pierreux, Mark A. Tarnopolsky, Louise Deldicque
Summary: The study investigated the effect of acute exercise on plasma concentrations of exosome-like vesicles (ELVs) and markers of multivesicular body (MVB) biogenesis in skeletal muscle. The results showed that acute aerobic exercise increased the plasma abundance of ELVs in healthy subjects under normoxia, but not in prediabetic subjects or under hypoxia. Skeletal muscle analysis suggested a minor role of this tissue in the exercise-induced increase in circulating ELVs.
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Qiaoyun Hou, Shuohua Chen, Qi An, Boya Li, Yan Fu, Yongzhang Luo
Summary: The study confirms plasma Hsp90 alpha as a biomarker for breast cancer, providing insights into its prognostic value across different clinical stages, and suggests that eHsp90 alpha plays a role in promoting lymphangiogenesis in breast cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Ya Cao, Xiaomeng Yu, Tianyu Zeng, Ziyi Fu, Yingyan Zhao, Beibei Nie, Jing Zhao, Yongmei Yin, Genxi Li
Summary: The study developed biomimetic vesicles for molecular classification of breast cancer exosomes. These vesicles specifically recognized and fused with breast cancer exosomes, amplifying electrochemical signaling using DNA machinery. Application to clinical samples demonstrated the feasibility and reliability of this method for diagnosis and treatment of breast cancer patients.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Analytical
Jonas M. Nikoloff, Mario A. Saucedo-Espinosa, Petra S. Dittrich
Summary: By analyzing the protein profiles of single extracellular vesicles (EVs), we found that EVs released by a single cell can represent its cell of origin. The microfluidic platform we used allowed for immobilization of EVs from individual cells, reducing the heterogeneity seen in cell populations. Through immunostaining and fluorescence microscopy, we determined the biochemical characteristics of membrane and cytosolic proteins in EVs. Our study demonstrates the potential of EVs as diagnostic markers and provides a method for studying EV biogenesis.
ANALYTICAL CHEMISTRY
(2023)
Article
Oncology
Yan Wang, Lili Du, Jiexian Jing, Xianwen Zhao, Xing Wang, Shenghuai Hou
Summary: Effective screening and treatment have reduced the number of women dying from breast cancer, but the long-term effects of treatment and psychosocial factors still affect the quality of life of patients. Therefore, the discovery and application of targeted biomarkers to improve functional outcome and life quality is necessary for breast cancer patients.
Article
Medicine, Research & Experimental
Xinjian Huang, Dongni Shi, Xuxiazi Zou, Xuxia Wu, Shumei Huang, Lingzhi Kong, Muwen Yang, Yunyun Xiao, Boyu Chen, Xiangfu Chen, Ying Ouyang, Libing Song, Yunting Jian, Chuyong Lin
Summary: This study found that BAG2 is significantly upregulated in relapse breast cancer patient tissues, and its high expression is associated with a worse prognosis. BAG2 localizes in mutant p53 aggregates and interacts with misfolded p53 mutants. BAG2 exacerbates the formation of the aggregates and recruits HSP90 to promote their propagation and maintenance, leading to chemoresistance in breast cancer.
Review
Cell Biology
Xi Wang, Chunxiao Sun, Xiang Huang, Jun Li, Ziyi Fu, Wei Li, Yongmei Yin
Summary: Breast cancer is a common and aggressive malignant tumor in women, and exosomes play crucial roles in its biology, participating in therapeutic resistance, invasion, and metastasis, as well as interacting with the tumor microenvironment.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Immunology
Laura Garcia-Estevez, Silvia Gonzalez-Martinez, Gema Moreno-Bueno
Summary: Breast cancer interacts with adipose tissue, leptin, and the immune system in a complex way, with specific immune cells playing important roles in breast cancer.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Oncology
Sreerenjini Lakshmi, Thomas A. Hughes, Sulochana Priya
Summary: Exosomes and their RNA have potential roles in breast cancer biology and treatment, participating in breast cancer tumorigenesis and progression through the transfer of miRNAs and LncRNAs. The diagnostic and therapeutic applications of exosomal miRNAs/LncRNAs in breast cancer have been highlighted in numerous publications.
EUROPEAN JOURNAL OF CANCER
(2021)
Article
Chemistry, Multidisciplinary
Jelle Penders, Anika Nagelkerke, Eoghan M. Cunnane, Simon Pedersen, Isaac J. Pence, R. Charles Coombes, Molly M. Stevens
Summary: The study focuses on extracellular vesicles (EVs) and introduces a high-throughput label-free EV analysis method for cancer diagnosis and monitoring, eliminating interpreter bias. By developing a dedicated device and using dimensional reduction analysis (DRA), a convenient and comprehensive approach for comparing multiple EV spectra is demonstrated. The study shows the high sensitivity and specificity of the dedicated SPARTA system in differentiating cancer and noncancer EVs, and its consistent predictive ability across multiple EV isolations.
Article
Virology
Yangli Wan, Yan Chen, Ting Wang, Bao Zhao, Wei Zeng, Leyan Zhang, Yanming Zhang, Shengyan Cao, Jingyu Wang, Qinghong Xue, Xuefeng Qi
Summary: Autophagy plays a crucial role in the pathogenesis of PPRV, while the involvement of exosomes in viral infections is gaining recognition. PPRV infection can lead to packaging of viral genetic material and proteins into exosomes, promoting transmission to new target cells. This study suggests a novel mechanism involving autophagy and exosome-mediated intercellular transmission of PPRV.
JOURNAL OF VIROLOGY
(2022)
Article
Chemistry, Analytical
Helisa H. Wippel, Juan D. Chavez, Andrew D. Keller, James E. Bruce
Summary: The XL-MS technique provides insight into protein conformations and interactions within their cellular environment, while the iqPIR strategy allows for comparative interactome studies using isotope encoded chemical cross-linkers. Multiplexed iqPIR enables quantitative interactome analysis of up to six biological samples in a single LC-MS acquisition, revealing specific protein conformational and interaction changes in response to different inhibitors.
ANALYTICAL CHEMISTRY
(2022)
Article
Cell Biology
Bharati Mendhe, Mohammad B. Khan, Damon Dunwody, Khairat Bahgat Youssef El Baradie, Kathryn Smith, Wenbo Zhi, Ashok Sharma, Tae Jin Lee, Mark W. Hamrick
Summary: Ischemia-reperfusion (I/R) injury is a common complication affecting multiple organs and tissues. Extracellular vesicles (EVs) derived from adipose stem cells may be a potential therapeutic resource for preventing tissue damage associated with I/R injury. However, while EVs may enhance reperfusion, they can also negatively impact muscle tissue and possibly remote organs. Alternative approaches, such as targeting mitochondrial permeability, may be more effective at mitigating I/R injury.
Article
Medical Laboratory Technology
Ting Zhu, Junjun Wang, Juan Li, Qichao Zhang, Yanyan Shang, Junhao Zhou, Ling Min, Bo Lv, Kai Luo
Summary: In this study, researchers identified a LncRNA signature associated with the prognosis of TNBC patients using RNA-sequencing analysis and qRT-PCR assay. The signature showed a significant correlation with shorter overall and progression-free survival time in the validation cohorts. Therefore, this signature has the potential to be a prognostic marker for TNBC.
CLINICA CHIMICA ACTA
(2023)
Article
Nutrition & Dietetics
Fabrizio Ceraudo, Giovanna Caparello, Angelo Galluccio, Ennio Avolio, Giuseppina Augimeri, Daniela De Rose, Adele Vivacqua, Catia Morelli, Ines Barone, Stefania Catalano, Cinzia Giordano, Diego Sisci, Daniela Bonofiglio
Summary: The study evaluated the impact of Mediterranean food choices on lipid and glycemic metabolic profile in adolescents. The results indicated that adopting a Mediterranean diet could significantly reduce glucose and lipid profile in healthy adolescents.
Review
Nutrition & Dietetics
Elisa Mattavelli, Elena Olmastroni, Daniela Bonofiglio, Alberico L. Catapano, Andrea Baragetti, Paolo Magni
Summary: The Mediterranean diet is a comprehensive lifestyle that promotes health and reduces the prevalence of chronic diseases. Its implementation is influenced by sociocultural and geographical factors, as well as personal food preferences and nutrition habits.
Editorial Material
Nutrition & Dietetics
Daniela Bonofiglio
Article
Multidisciplinary Sciences
Mohamed A. Abu El Maaty, Julie Terzic, Celine Keime, Daniela Rovito, Regis Lutzing, Darya Yanushko, Maxime Parisotto, Elise Grelet, Izzie Jacques Namer, Veronique Lindner, Gilles Laverny, Daniel Metzger
Summary: This study reveals the role of hypoxia-inducible factor 1 alpha (HIF1A) in promoting the malignant progression of prostatic intraepithelial neoplasia (PIN). It shows that early PINs are hypoxic and that luminal HIF1A enhances this hypoxic environment, leading to impaired immune surveillance and the emergence of cells overexpressing Transglutaminase 2 (TGM2) with impaired androgen signaling. Elevated TGM2 levels are associated with shortened progression-free survival in patients with prostate cancer. Furthermore, pharmacologically inhibiting HIF1A impairs cell proliferation and induces apoptosis in PINs. These findings highlight HIF1A as a potential target for PCa prevention and TGM2 as a promising prognostic biomarker for early relapse after prostatectomy.
Article
Food Science & Technology
Serena Carpentieri, Giuseppina Augimeri, Jessica Ceramella, Adele Vivacqua, Maria Stefania Sinicropi, Gianpiero Pataro, Daniela Bonofiglio, Giovanna Ferrari
Summary: The study utilized pulsed electric field pretreatment to enhance the extractability of valuable compounds from artichoke by-products, demonstrating the antioxidant and anti-inflammatory properties of the extracts. Biological effects on THP-1 macrophages were investigated.
Article
Biochemistry & Molecular Biology
Luca Gelsomino, Ines Barone, Amanda Caruso, Francesca Giordano, Matteo Brindisi, Giovanna Morello, Felice Maria Accattatis, Salvatore Panza, Anna Rita Cappello, Daniela Bonofiglio, Sebastiano Ando, Stefania Catalano, Cinzia Giordano
Summary: Tumor extracellular vesicles (EVs) play a crucial role in cell-to-cell communication in breast cancer by transporting nucleic acids, proteins, and metabolites. Leptin induces the release of EVs from breast cancer cells, and these vesicles have a specific proteomic signature related to mitochondrial processes and activity. Lep-EVs increase ATP levels and modulate the oxidative metabolism of breast cancer cells. Furthermore, Lep-EVs sustain the polarization of macrophages in the tumor microenvironment (TME) towards a tumor-promoting phenotype.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Oncology
Michele Pellegrino, Elena Ricci, Rosangela Ceraldi, Alessandra Nigro, Daniela Bonofiglio, Marilena Lanzino, Catia Morelli
Summary: This review highlights the anti-tumor activity of several antiepileptic drugs in breast, prostate, and other cancers. It focuses on their ability to block ion channels and inhibit histone deacetylases, which are well-established tumor markers and molecular targets. The repurposing of already approved drugs as adjuvants in cancer treatment is advantageous due to reduced toxicity and faster clinical approval. The review also discusses the attempt to administer antiepileptic drugs through drug delivery systems to improve their effectiveness and reduce toxicity.
Article
Oncology
Giuseppina Augimeri, Marco Fiorillo, Catia Morelli, Salvatore Panza, Cinzia Giordano, Ines Barone, Stefania Catalano, Diego Sisci, Sebastiano Ando, Daniela Bonofiglio
Summary: Triple-negative breast cancer (TNBC), lacking effective targeted therapies, can be attenuated by DHEA through reducing cell migration, invasion, and metabolic activity. In addition, DHEA suppresses macrophage recruitment by reducing CCL5 secretion from TNBC cells, providing a potential treatment option against TNBC.
Review
Biochemistry & Molecular Biology
Giuseppina Augimeri, Daniela Bonofiglio
Summary: Unhealthy dietary habits increase the risk of cancer, and adopting a healthy eating pattern, such as the Mediterranean diet high in omega-3 polyunsaturated fatty acids (N-3 PUFAs), is recommended to prevent various types of cancers, including breast cancer. The consumption of N-3 PUFAs, particularly EPA and DHA, reduces the risk and progression of breast cancer, and also helps counteract side effects of chemotherapy. DHA, in particular, has shown significant antitumoral effects in breast cancer cells and its derivative DHEA has potential therapeutic benefits.
Article
Geriatrics & Gerontology
Kamar Ghaibour, Melanie Schuh, Sirine Souali-Crespo, Celine Chambon, Anouk Charlot, Joe Rizk, Daniela Rovito, Anna-Isavella Rerra, Qingshuang Cai, Nadia Messaddeq, Joffrey Zoll, Delphine Duteil, Daniel Metzger
Summary: Disrupting the androgen/androgen receptor axis impairs glycolytic activity and promotes the development of type 2 diabetes in male mice, but not in females. It also leads to oxidative stress and necrosis in muscle fibers.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2023)
Article
Chemistry, Multidisciplinary
Giovanna Caparello, Angelo Galluccio, Fabrizio Ceraudo, Claudio Pecorella, Fabio Buzzanca, Francesco Cuccomarino, Daniela Bonofiglio, Ennio Avolio
Summary: An optimal body composition in volleyball players can be achieved by monitoring training plans and nutritional habits, and the Mediterranean Diet (MD) is a good choice to meet the nutritional needs of athletes. This study used bioelectrical impedance vector analysis (BIVA) to monitor body composition changes in volleyball players following MD recommendations. The results showed that athletes maintained an optimal MD adherence and experienced significant changes in BIVA parameters, indicating improved body cell mass and skeletal muscle mass. These findings support the use of BIVA as a tool to monitor athlete's body adaptations and highlight the importance of the MD in sports settings.
APPLIED SCIENCES-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Cinzia Giordano, Felice Maria Accattatis, Luca Gelsomino, Piercarlo Del Console, Balazs Gyorffy, Mario Giuliano, Bianca Maria Veneziani, Grazia Arpino, Carmine De Angelis, Pietro De Placido, Erica Pietroluongo, Francesco Zinno, Daniela Bonofiglio, Sebastiano Ando, Ines Barone, Stefania Catalano
Summary: This study identified novel miRNA signatures, miRNA-27a and miRNA-128, in breast cancer by analyzing the expression profiles of EV-derived miRNAs in the blood circulation. These miRNAs showed diagnostic accuracy in distinguishing breast cancer patients from non-cancer controls.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Giuseppina Augimeri, Marco Fiorillo, Giovanna Caparello, Fabrizio Ceraudo, Ennio Avolio, Catia Morelli, Ines Barone, Stefania Catalano, Sebastiano Ando, Cinzia Giordano, Diego Sisci, Adele Vivacqua, Daniela Bonofiglio
Summary: This study found that the COVID-19 lockdown had a negative impact on the metabolic and inflammatory profile of adolescents, despite no significant differences in anthropometric measurements and adherence to the Mediterranean diet. Post-lockdown adolescents had higher levels of fasting glucose, triglycerides, total cholesterol, LDL, tumor necrosis factor (TNF)-alpha, interleukin-1 beta, and ferritin, along with lower levels of high-density lipoprotein (HDL). The study also identified several serum markers as predictors for metabolic and inflammatory profiling after the lockdown period.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Article
Endocrinology & Metabolism
Simona De Angelis, Emanuela Medda, Daniela Rotondi, Maria Masocco, Valentina Minardi, Benedetta Contoli, Valentina Possenti, Angela Sorbo, Marilena D'Amato, Anna Chiara Turco, Alberto Augusto Pastorelli, Paolo Stacchini, Roberto Da Cas, Marcello Bagnasco, Daniela Bonofiglio, Maurizio Gasperi, Domenico Meringolo, Caterina Mian, Mariacarla Moleti, Francesco Vermiglio, Efisio Puxeddu, Augusto Taccaliti, Massimo Tonacchera, Salvatore Ulisse, Antonio Dimida, Teresa Rago, Elena Nazzari, Mara Schiavo, Irene Bossert, Giacomo Sturniolo, Carlo Corbetta, Cristina Cereda, Laura Cappelletti, Marta Camilot, Francesca Teofoli, Renzo Ciatti, Elisabetta Tarsi, Nicola Perrotti, Onorina Marasco, Giovanna Scozzafava, Francesca Righetti, Sebastiano Ando, Stefania Catalano, Mariarosaria Cristofaro, Giovanni Sorrenti, Simona Censi, Silvia Morelli, Enke Baldini, Giuseppe Plutino, Roberto Copparoni, Elena Alonzo, Claudia Giacomozzi, Marco Silano, Antonella Olivieri
Summary: This study evaluated the efficiency and adverse effects of the iodine prophylaxis program in Italy. The results showed that Italy has achieved iodine sufficiency through the program, but there are still concerns about iodine nutritional status during pregnancy.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Meeting Abstract
Rheumatology
Margherita Giannini, Daniela Rovito, Lea Debrut, Celine Keime, Beatrice Lannes, Anne-Laure Charles, Delphine Duteil, Bernard Geny, Daniel Metzger, Gilles Laverny, Alain Meyer
ARTHRITIS & RHEUMATOLOGY
(2022)