Article
Immunology
Shakti Singh, Sunna Nabeela, Ashley Barbarino, Ashraf S. Ibrahim, Priya Uppuluri
Summary: Prevention of disseminated candidiasis in neonates is important and vaccination with recombinant forms of the C. albicans N-termini of cell wall proteins Als3 and Hyr1, as well as the transfer of antibodies, can offer protection. These findings highlight the potential clinical relevance of active and passive vaccination strategies using these antigens.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biology
Asier Garcia-Senosiain, Ikhlaq Hussain Kana, Subhash Singh, Manoj Kumar Das, Morten Hanefeld Dziegiel, Sanne Hertegonne, Bright Adu, Michael Theisen
Summary: Garcia-Senosiain et al developed an in vitro opsonic phagocytosis assay using peripheral blood leukocytes and found that peripheral blood neutrophils are the main phagocytes of P. falciparum blood-stage merozoites.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jonothan Rosario-Colon, Karen Eberle, Abby Adams, Evan Courville, Hong Xin
Summary: This study introduced a novel approach to treating C. auris disseminated infections through passive transfer of monoclonal antibodies targeting specific antigens, which showed significantly enhanced survival and reduction of fungal burdens. The combination of beta-Man(3)-specific mAb and peptide-specific mAbs demonstrated greater protection compared to individual mAb treatment against medically important Candida species, including multidrug-resistant C. auris.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Virology
Giuditta De Lorenzo, Rapeepat Tandavanitj, Jennifer Doig, Chayanee Setthapramote, Monica Poggianella, Ricardo Sanchez-Velazquez, Hannah E. Scales, Julia M. Edgar, Alain Kohl, James Brewer, Oscar R. Burrone, Arvind H. Patel
Summary: The study developed ZIKV subunit and virus-like particle (VLP)-based vaccines, which induced antibodies with a higher capacity to neutralize virus infection and protected animals from lethal challenge with both the African and Asian lineages of ZIKV. The engineered immunogens reduced the potential to induce antibody-dependent enhancement (ADE) of infection and showed promising efficacy in comparison with the wild-type derivatives.
JOURNAL OF VIROLOGY
(2021)
Article
Immunology
Marianna Agudelo, Martin Palus, Jennifer R. Keeffe, Filippo Bianchini, Pavel Svoboda, Jiri Salat, Avery Peace, Anna Gazumyan, Melissa Cipolla, Tania Kapoor, Francesca Guidetti, Kai-Hui Yao, Jana Elsterova, Dana Teislerova, Ales Chrdle, Vaclav Honig, Thiago Oliveira, Anthony P. West, Yu E. Lee, Charles M. Rice, Margaret R. MacDonald, Pamela J. Bjorkman, Daniel Ruzek, Davide F. Robbiani, Michel C. Nussenzweig
Summary: The study revealed the human neutralizing antibody response to TBEV, showing that the most potent neutralizing antibodies were found in individuals who recovered from natural infection. These antibodies also neutralized other tick-borne flaviviruses, and structural analysis identified a conserved epitope near the lateral ridge of EDIII. Prophylactic or early therapeutic antibody administration was effective at low doses in mice lethally infected with TBEV.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Multidisciplinary Sciences
Tal Noy-Porat, Adva Mechaly, Yinon Levy, Efi Makdasi, Ron Alcalay, David Gur, Moshe Aftalion, Reut Falach, Shani Leviatan Ben-Arye, Shirley Lazar, Ayelet Zauberman, Eyal Epstein, Theodor Chitlaru, Shay Weiss, Hagit Achdout, Jonathan D. Edgeworth, Raghavendra Kikkeri, Hai Yu, Xi Chen, Shmuel Yitzhaki, Shmuel C. Shapira, Vered Padler-Karavani, Ohad Mazor, Ronit Rosenfeld
Summary: The study isolated and characterized 12 neutralizing human mAbs targeting three distinct epitopes on the spike N-terminal domain of the SARS-CoV-2 virus. Two selected mAbs demonstrated full protection of mice when administered at low doses and late postexposure, showing high potential for therapy of SARS-CoV-2 infection.
Article
Immunology
Panpan Zhou, Ge Song, Hejun Liu, Meng Yuan, Wan-ting He, Nathan Beutler, Xueyong Zhu, Longping V. Tse, David R. Martinez, Alexandra Schafer, Fabio Anzanello, Peter Yong, Linghang Peng, Katharina Dueker, Rami Musharrafieh, Sean Callaghan, Tazio Capozzola, Oliver Limbo, Mara Parren, Elijah Garcia, Stephen A. Rawlings, Davey M. Smith, David Nemazee, Joseph G. Jardine, Yana Safonova, Bryan Briney, Thomas F. Rogers, Ian A. Wilson, Ralph S. Baric, Lisa E. Gralinski, Dennis R. Burton, Raiees Andrabi
Summary: Research finds that specific neutralizing antibodies play a key role in developing broadly protective vaccines against novel pandemic coronaviruses and effectively responding to SARS-CoV-2 variants. A panel of broadly neutralizing antibodies targeting a conserved S2 region in the betacoronavirus spike fusion machinery was isolated from vaccinated-recovered donors. These antibodies showed broad in vivo protection against three deadly betacoronaviruses and provided insights for developing pan-betacoronavirus vaccines.
Article
Microbiology
Andrea M. DiVenere, Dzifa Amengor, Rui P. Silva, Jory A. Goldsmith, Jason S. McLellan, Jennifer A. Maynard
Summary: This study demonstrates the protective role of neutralizing antibodies against ACT in pertussis infection. These antibodies can synergistically interact with antibodies against current vaccine antigens, providing protection against pertussis infection.
Article
Biotechnology & Applied Microbiology
Jiao Guo, Haiyu Yang, Ya Liu, Wei Liu, Ruiying Zhao, He Li, Wei Long, Wenqing Xu, Meili Guo, Xiaodong Zhang
Summary: In this study, atomically precise Ag-14 clusterzymes were developed and proven to be effective radioprotectants both in vitro and in vivo. These clusterzymes exhibited unique superoxide dismutase-like activity and strong anti-oxidative abilities. They improved cell viability by eliminating ROS and preventing DNA damage, as well as enhanced the survival rate of irradiated mice by protecting hematological systems and repairing tissue oxidative stress damage. Additionally, the biodistribution and toxicological experiments showed minimal toxic responses in mice up to 30 days.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Anne M. Talkington, Morgan D. McSweeney, Tao Zhang, Zibo Li, Andrew C. Nyborg, Brian LaMoreaux, Eric W. Livingston, Jonathan E. Frank, Hong Yuan, Samuel K. Lai
Summary: Pre-infusion of high MW free PEG (40 kDa) can serve as a decoy to saturate circulating APA, preventing subsequent doses of pegloticase from being eliminated from the circulation rapidly by APA. This strategy may offer a promising approach to enable safe and effective use of pegloticase in patients with treatment-refractory gout who have developed anti-PEG antibodies.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Medicine, Research & Experimental
Milad Reda Qelliny, Taro Shimizu, Nehal E. Elsadek, Sherif E. Emam, Haruka Takata, Zeinab M. A. Fathalla, Amal K. Hussein, Khaled A. Khaled, Hidenori Ando, Yu Ishima, Tatsuhiro Ishida
Summary: The copresentation of gangliosides and DNA on PCLs did not reduce anti-DNA IgM production, but significantly reduced anti-PEG IgM production.
MOLECULAR PHARMACEUTICS
(2021)
Article
Multidisciplinary Sciences
Jianliang Xu, Kai Xu, Seolkyoung Jung, Andrea Conte, Jenna Lieberman, Frauke Muecksch, Julio Cesar Cetrulo Lorenzi, Solji Park, Fabian Schmidt, Zijun Wang, Yaoxing Huang, Yang Luo, Manoj S. Nair, Pengfei Wang, Jonathan E. Schulz, Lino Tessarollo, Tatsiana Bylund, Gwo-Yu Chuang, Adam S. Olia, Tyler Stephens, I-Ting Teng, Yaroslav Tsybovsky, Tongqing Zhou, Vincent Munster, David D. Ho, Theodora Hatziioannou, Paul D. Bieniasz, Michel C. Nussenzweig, Peter D. Kwong, Rafael Casellas
Summary: The study found that camelid nanobodies can effectively circumvent vaccine escape caused by mutations in the novel coronavirus. These nanobodies are able to neutralize SARS-CoV-2 variants through two mechanisms, demonstrating promising potential in preventing COVID-19 mortality when vaccines are compromised.
Article
Immunology
Mo Zhou, Jun Xie, Osamu Kawase, Yoshifumi Nishikawa, Shengwei Ji, Shanyuan Zhu, Shinuo Cao, Xuenan Xuan
Summary: This study found that there were significant anti-erythrocyte and anti-platelet autoantibodies in mice infected with Plasmodium and Babesia parasites, which may contribute to thrombocytopenia and hemolytic anemia. This research will help to understand the mechanisms of malaria and babesiosis-related thrombocytopenia and hemolytic anemia.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Medicine, Research & Experimental
Frederic Depreux, Lyubov Czech, Hunter Young, Claus-Peter Richter, Yingjie Zhou, Donna S. Whitlon
Summary: This article evaluates the potential of statin drugs in the treatment of noise-induced hearing loss. The effects of direct injection and oral administration of the drugs were assessed. The results show that statins can protect mice from noise-induced hearing damage.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Silvia Bozza, Rossana Giulietta Iannitti, Marilena Pariano, Giorgia Renga, Claudio Costantini, Luigina Romani, Jagadeesh Bayry
Summary: The study assessed the potential therapeutic efficacy of a CCR4 small molecule antagonist against various settings of fungal infection, demonstrating effectiveness in different scenarios. The results suggest the need for developing novel compounds with potential for clinical trials in the field of CCR4 antagonism.
Article
Multidisciplinary Sciences
Felix Mba Medie, Batu K. Sharma-Kuinkel, Felicia Ruffin, Liana C. Chan, Maura Rossetti, Yu-Ling Chang, Lawrence P. Park, Arnold S. Bayer, Scott G. Filler, Richard Ahn, Elaine F. Reed, David Gjertson, Michael R. Yeaman, Vance G. Fowler
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Genetics & Heredity
Katherine Lagree, Carol A. Woolford, Manning Y. Huang, Gemma May, C. Joel McManus, Norma V. Solis, Scott G. Filler, Aaron P. Mitchell
Article
Genetics & Heredity
Amol C. Shetty, John Mattick, Matthew Chung, Carrie McCracken, Anup Mahurkar, Scott G. Filler, Claire M. Fraser, David A. Rasko, Vincent M. Bruno, Julie C. Dunning Hotopp
MICROBIAL GENOMICS
(2020)
Article
Microbiology
Teclegiorgis Gebremariam, Sondus Alkhazraji, Abdullah Alqarihi, Nathan P. Wiederhold, Karen Joy Shaw, Thomas F. Patterson, Scott G. Filler, Ashraf S. Ibrahim
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2020)
Article
Genetics & Heredity
Selma S. Alkafeef, Shelley Lane, Clinton Yu, Tingting Zhou, Norma Solis, Scott G. Filler, Lan Huang, Haoping Liu
Article
Immunology
Elan E. Filler, Yaoping Liu, Norma Solis, Ling Wang, Luis F. Diaz, John E. Edwards, Scott G. Filler, Michael R. Yeaman
Summary: Histone modification plays a crucial role in enabling Candida glabrata to survive host defense peptides and caspofungin, with Ada2 and Rpd3 being essential for the maximal virulence of this organism during dissemination infection.
INFECTION AND IMMUNITY
(2021)
Article
Microbiology
Quynh T. Phan, Jianfeng Lin, Norma Solis, Michael Eng, Marc Swidergall, Feng Wang, Shan Li, Sarah L. Gaffen, Tsui-Fen Chou, Scott G. Filler
Summary: The study reveals that several proteins associated with EGFR play a central role in the response of human oral epithelial cells to C. albicans infection, with gC1qR functioning as a key coreceptor which is necessary for endocytosis of the fungus and secretion of proinflammatory mediators by the cells. This work provides valuable insights into the EGFR signaling pathways in the context of C. albicans infection.
Article
Microbiology
Rocio Garcia-Rodas, Hayet Labbaoui, Francois Orange, Norma Solis, Oscar Zaragoza, Scott G. Filler, Martine Bassilana, Robert A. Arkowitz
Summary: This study reveals that plasma membrane PI(4)P plays a central role in filamentation, cell wall integrity, and virulence in Candida albicans.
Article
Microbiology
Mengli Yang, Norma V. Solis, Michaela Marshall, Rachel Garleb, Tingting Zhou, Daidong Wang, Marc Swidergall, Eric Pearlman, Scott G. Filler, Haoping Liu
Summary: Candida albicans, a major opportunistic pathogen, can switch between different forms and regulate immune recognition during morphogenesis. This study reveals that two highly expressed yeast proteins, Eng1 and Ywp1, mask beta-glucan in the cell wall, but beta-glucan is exposed during yeast-to-hypha transition. The expression of Eng1 is dynamically regulated in response to different carbon sources and antifungal drugs. The eng1 mutant modulates virulence in a sex and Dectin-1-dependent manner in a mouse model of disseminated candidiasis. These findings highlight the importance of beta-glucan exposure in modulating the balance between immune protection and immunopathogenesis during candidiasis.
Editorial Material
Microbiology
Jianfeng Lin, Scott G. Filler
NATURE MICROBIOLOGY
(2021)
Article
Microbiology
Quynh T. Phan, Norma Solis, Jianfeng Lin, Marc Swidergall, Shakti Singh, Hong Liu, Donald C. Sheppard, Ashraf S. Ibrahim, Aaron P. Mitchell, Scott G. Filler
Summary: The invasion of vascular endothelial cells by Candida spp. is an important step in the pathogenesis of hematogenously disseminated candidiasis. This study reveals that Candida glabrata and other Candida spp. can adhere to and invade human endothelial cells by using the serum proteins kininogen and vitronectin as bridging molecules. These serum proteins interact with the globular C1q receptor (gC1qR) and alpha v integrins on the endothelial cell surface, inducing endocytosis. This mechanism is not supported by mouse endothelial cells, but can be restored in mouse endothelial cells expressing human gC1qR or integrin alpha v. Therefore, bridging molecule-mediated endocytosis is a common mechanism by which medically important Candida spp. invade human vascular endothelial cells.
Article
Microbiology
Anupam Sharma, Norma V. Solis, Manning Y. Y. Huang, Frederick Lanni, Scott G. Filler, Aaron P. Mitchell
Summary: The HGC1 gene encodes a protein that is essential for the virulence of Candida albicans. It is responsible for the production of elongated hyphal filaments that are associated with virulence. However, HGC1 is not the sole gene involved in hypha formation, as other cyclins also play a role.
Article
Microbiology
Karen T. Graf, Hong Liu, Scott G. Filler, Vincent M. Bruno
Summary: Aspergillus fumigatus is an environmental fungus that can cause life-threatening pulmonary disease. The fungus interacts with epithelial cells in the small airways and alveoli of the lungs, disrupting the innate immune response by depleting proinflammatory chemokines necessary for host defense. This depletion is caused by melanin, a key constituent of the fungus' cell wall.
Article
Microbiology
Hong Liu, Amol C. Shetty, Ashraf S. Ibrahim, Scott G. Filler, Vincent M. Bruno
Summary: Invasive aspergillosis occurs when Aspergillus fumigatus invades the pulmonary epithelial cells. Transcriptome sequencing revealed that A549 alveolar epithelial cells activate various host cell signaling pathways upon infection, including tumor necrosis factor, interleukin, and Toll-like receptor pathways. Additionally, platelet-derived growth factor and progesterone receptor pathways are also activated by A. fumigatus.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Christina A. Cuomo, Saranna Fanning, Sharvari Gujja, Qiandong Zeng, Julian R. Naglik, Scott G. Filler, Aaron P. Mitchell
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2019)