Development of a CRISPR/Cas9-Based Tool for Gene Deletion in Issatchenkia orientalis

A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae

(2019) Yueping Zhang et al.   Nature Communications

Development of a CRISPR/Cas9 system for high efficiency multiplexed gene deletion in Rhodosporidium toruloides

(2019) J. Carl Schultz et al.   BIOTECHNOLOGY AND BIOENGINEERING

Engineered CRISPR/Cas9 system for multiplex genome engineering of polyploid industrial yeast strains

(2018) Jiazhang Lian et al.   BIOTECHNOLOGY AND BIOENGINEERING

Low-pH production of d -lactic acid using newly isolated acid tolerant yeast Pichia kudriavzevii NG7

(2018) Hyun Joo Park et al.   BIOTECHNOLOGY AND BIOENGINEERING

Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision

(2018) Zehua Bao et al.   NATURE BIOTECHNOLOGY

5S rRNA Promoter for Guide RNA Expression Enabled Highly Efficient CRISPR/Cas9 Genome Editing in Aspergillus niger

(2018) Xiaomei Zheng et al.   ACS Synthetic Biology

T7 Polymerase Expression of Guide RNAs in vivo Allows Exportable CRISPR-Cas9 Editing in Multiple Yeast Hosts

(2018) Nicholas J. Morse et al.   ACS Synthetic Biology

Population genomics shows no distinction between pathogenic Candida krusei and environmental Pichia kudriavzevii: One species, four names

(2018) Alexander P. Douglass et al.   PLoS Pathogens

Extraction of genomic DNA from yeasts for PCR-based applications

(2018) Marko Lõoke et al.   BIOTECHNIQUES

Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system

(2017) Jiazhang Lian et al.   Nature Communications

SgRNA Expression of CRIPSR-Cas9 System Based on MiRNA Polycistrons as a Versatile Tool to Manipulate Multiple and Tissue-Specific Genome Editing

(2017) Chen Xie et al.   Scientific Reports

Rapid Isolation of Centromeres from Scheffersomyces stipitis

(2017) Mingfeng Cao et al.   ACS Synthetic Biology

Centromeric DNA Facilitates Nonconventional Yeast Genetic Engineering

(2017) Mingfeng Cao et al.   ACS Synthetic Biology

Multiplex gene editing of the Yarrowia lipolytica genome using the CRISPR-Cas9 system

(2016) Shuliang Gao et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Synthetic RNA Polymerase III Promoters Facilitate High-Efficiency CRISPR–Cas9-Mediated Genome Editing in Yarrowia lipolytica

(2016) Cory M. Schwartz et al.   ACS Synthetic Biology

Off-target Effects in CRISPR/Cas9-mediated Genome Engineering

(2015) Xiao-Hui Zhang et al.   Molecular Therapy-Nucleic Acids

Exploiting Issatchenkia orientalis SD108 for succinic acid production

(2014) Han Xiao et al.   Microbial Cell Factories

Homology-Integrated CRISPR–Cas (HI-CRISPR) System for One-Step Multigene Disruption in Saccharomyces cerevisiae

(2014) Zehua Bao et al.   ACS Synthetic Biology

Self-processing of ribozyme-flanked RNAs into guide RNAsin vitroandin vivofor CRISPR-mediated genome editing

(2013) Yangbin Gao et al.   Journal of Integrative Plant Biology

Time to Be Versatile: Regulation of the Replication Timing Program in Budding Yeast

(2013) Kazumasa Yoshida et al.   JOURNAL OF MOLECULAR BIOLOGY

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

(2013) James E. DiCarlo et al.   NUCLEIC ACIDS RESEARCH

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

FairyTALE: A High-Throughput TAL Effector Synthesis Platform

(2013) Jing Liang et al.   ACS Synthetic Biology

Centromere-Like Regions in the Budding Yeast Genome

(2013) Philippe Lefrançois et al.   PLoS Genetics

Eukaryotic DNA replication origins: many choices for appropriate answers

(2010) Marcel Méchali   NATURE REVIEWS MOLECULAR CELL BIOLOGY

DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways

(2008) Zengyi Shao et al.   NUCLEIC ACIDS RESEARCH