Article
Immunology
Bo Deng, Yuli Lin, Yusheng Chen, Shuai Ma, Qian Cai, Wenji Wang, Bingji Li, Tingyan Liu, Peihui Zhou, Rui He, Feng Ding
Summary: The study revealed that pDCs play a detrimental role in AKI by producing IFN-alpha, inducing apoptosis of renal tubular epithelial cells, promoting immune cell infiltration and proinflammatory cytokine production, exacerbating kidney damage. The results indicate that IFN-alpha is a key factor mediating the functions of pDCs in AKI pathogenesis.
CELLULAR & MOLECULAR IMMUNOLOGY
(2021)
Article
Immunology
Paoline Laurent, Chao Yang, Andre F. Rendeiro, Benjamin E. Nilsson-Payant, Lucia Carrau, Vasuretha Chandar, Yaron Bram, Benjamin R. tenOever, Olivier Elemento, Lionel B. Ivashkiv, Robert E. Schwartz, Franck J. Barrat
Summary: Lung-infiltrating macrophages play a role in the inflammation and lethality of COVID-19, despite not being infected by the virus. Plasmacytoid dendritic cells (pDCs) in the lungs produce interferons (IFN-I) in response to SARS-CoV-2, leading to macrophage activation. This interaction between pDCs and macrophages may contribute to the severity of the disease.
SCIENCE IMMUNOLOGY
(2022)
Article
Immunology
Kerstin H. Mair, Maria Stadler, Mahsa Adib Razavi, Armin Saalmuller, Wilhelm Gerner
Summary: This study demonstrates the expression and function of NKp46 on porcine plasmacytoid dendritic cells (pDCs). It was found that the majority of porcine pDCs express NKp46 and have an active signaling pathway upon NKp46 triggering, leading to cytokine production. However, unlike NK cells, NKp46 on porcine pDCs does not seem to be involved in cytotoxicity.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Rheumatology
Ana Paula Rosa dos Santos, Camila de Oliveira Vaz, Bidossessi Wilfried Hounkpe, Bruna Cardoso Jacintho, Jose Diogo Oliveira, Gabriela Lisiane Tripiquia Vechiatto Mesquita, Irene Pereira dos Santos, Joyce Annichino-Bizzacchi, Simone Appenzeller, Bruna de Moraes Mazetto Fonseca, Fernanda Andrade Orsi
Summary: This study found an association between plasmacytoid dendritic cells and interferon (IFN)-I with thrombotic antiphospholipid syndrome (APS), providing a new mechanism for the pathophysiology of APS.
Article
Immunology
Tae Jin Yun, Suzu Igarashi, Haoquan Zhao, Oriana A. Perez, Marcus R. Pereira, Emmanuel Zorn, Yufeng Shen, Felicia Goodrum, Adeeb Rahman, Peter A. Sims, Donna L. Farber, Boris Reizis
Summary: Plasmacytoid dendritic cells (pDCs) can recognize live infected cells and produce a unique antiviral immune response, including high amounts of interferons, prolonged and efficient responses to CMV-infected cells, and activation of natural killer cells. Patients with CMV viremia show activated pDCs and increased circulating IFN-I and IFN-III.
SCIENCE IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Meiling Zheng, Zhi Hu, Xiaole Mei, Lianlian Ouyang, Yang Song, Wenhui Zhou, Yi Kong, Ruifang Wu, Shijia Rao, Hai Long, Wei Shi, Hui Jing, Shuang Lu, Haijing Wu, Sujie Jia, Qianjin Lu, Ming Zhao
Summary: This study compares the differences between cutaneous lesions from DLE and SLE patients and healthy controls using single-cell RNA sequencing data. The findings reveal differential immune cell recruitment, cell type, and gene expression, highlighting potential therapeutic targets for lupus erythematosus.
NATURE COMMUNICATIONS
(2022)
Review
Immunology
Binhui Zhou, Toby Lawrence, Yinming Liang
Summary: Plasmacytoid dendritic cells (pDCs) are a special subtype of dendritic cells that produce massive amounts of type I interferon (IFN-I) and play a pivotal role in antiviral immunity. However, in many types of cancer, tumor-infiltrating pDCs do not respond to Toll-like receptor (TLR) stimulation, leading to reduced IFN-alpha production and promoting tumor growth.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Laura C. Van Eyndhoven, Eleni Chouri, Nikita Subedi, Jurjen Tel
Summary: Plasmacytoid dendritic cells (pDCs) are a rare type of immune cells with specialized functions in producing large amounts of type I interferon (IFN-I) and exerting cytotoxic effects. Studies show that only a small percentage of pDCs respond to activation by toll-like receptors with IFN alpha secretion and upregulation of cytotoxic markers. The early IFN-producing pDCs, known as first responders, exhibit high expression of PD-L1 and TRAIL, while IFN-I primed cells increase IFN alpha production but do not significantly enhance cytotoxicity. Through droplet-based microfluidics, researchers observed that first responders play a key role in orchestrating population-wide IFN-I responses and have a high cytotoxic potential.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Virology
Jonas L. Delva, Cliff Van Waesberghe, Wim Van den Broeck, Jochen A. Lamote, Nick Vereecke, Sebastiaan Theuns, Liesbeth Couck, Herman W. Favoreel
Summary: The PRV vaccine strain Bartha plays a critical role in eradicating the disease in many countries, but its interaction with host cells and the immune system remains poorly understood. Bartha-infected epithelial cells produce increased amounts of infectious virus, leading to pDC hyperactivation. This phenotype is caused by the deletion of genes encoding US2 and gE/gI.
JOURNAL OF VIROLOGY
(2022)
Article
Immunology
Marie Dominique Ah Kioon, Michael Pierides, Tania Pannellini, Gang Lin, Carl F. Nathan, Franck J. Barrat
Summary: In this study, a novel inhibitor of the immunoproteasome, PKS3053, was used to show that it can effectively reduce immune cell activation without causing harm, holding promise as a potential therapy for inflammatory skin diseases.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kenjiro Takao, Makiko Suzuki, Ryo Miyazaki, Minoru Miyake, Kazuya Akimitsu, Katsuaki Hoshino
Summary: D-Allose, a rare sugar, has been found to inhibit cytokine production by murine dendritic cells stimulated with ssRNA or CpG DNA by attenuating the phosphorylation of MAPK family members.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Ge Chen, Yiming Zhang, Yaoyao Zhang, Jihui Ai, Bin Yang, Mengge Cui, Qiuyue Liao, Hanxiao Chen, Hualin Bai, Dashing Shang, Jing Chen, ChaoYang Sun, Haiyi Liu, Fengyuan Liu, Bin Mao, Guoqiang Sun, Lu Chen, Jing-wen Lin, Kezhen Li
Summary: Pregnant women infected with COVID-19 exhibit different immune characteristics, such as lymphopenia, increased inflammation, and heightened activation and chemotaxis of immune cells. Single-cell mRNA sequencing analysis revealed normal clonal expansion of T cells, increased activation of NK, NKT, and MAIT cells, and differential interferon responses in monocytes in pregnant patients.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2021)
Review
Dermatology
Tara Bardawil, Samar Khalil, Mazen Kurban, Ossama Abbas
Summary: Plasmacytoid dendritic cells infiltrate the skin in various infections, inflammations, and tumors, and can be identified in tissue using specific markers. Evaluating specific plasmacytoid dendritic cell-related parameters is a useful tool for differentiating skin diseases with overlapping features.
INDIAN JOURNAL OF DERMATOLOGY VENEREOLOGY & LEPROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Rebeca Arroyo Hornero, Juliana Idoyaga
Summary: Since their discovery, the identity and classification of plasmacytoid dendritic cells (pDCs) have been a subject of debate in the field. pDCs are distinct from other dendritic cell family members and may have dual origin from myeloid and lymphoid progenitors. They have the unique ability to quickly secrete abundant levels of type I interferon in response to viral infections and can activate T cells independently. This article provides an overview of the historic and current understanding of pDCs and argues for their inclusion within the dendritic cell network due to their role in linking innate and adaptive immune responses.
MOLECULAR IMMUNOLOGY
(2023)
Article
Microbiology
Courtney R. Plumlee, Fergal J. Duffy, Benjamin H. Gern, Jared L. Delahaye, Sara B. Cohen, Caleb R. Stoltzfus, Tige R. Rustad, Scott G. Hansen, Michael K. Axthelm, Louis J. Picker, John D. Aitchison, David R. Sherman, Vitaly V. Ganusov, Michael Y. Gerner, Daniel E. Zak, Kevin B. Urdahl
Summary: The study successfully developed a tuberculosis model in mice that more closely resembles human disease by infecting them with an ultra-low dose of bacteria, resulting in highly heterogeneous bacterial burdens and granulomas similar to those in humans. By identifying blood RNA signatures, it was possible to predict infection outcomes in mice and infer the risk of progression to active tuberculosis in humans.
CELL HOST & MICROBE
(2021)
Article
Multidisciplinary Sciences
Jana Freff, Kathrin Schwarte, Lisa Broeker, Judith Buehlmeier, Isabelle Kraft, Dana Oeztuerk, Anke Hinney, Volker Arolt, Udo Dannlowski, Georg Romer, Bernhard T. Baune, Johannes Hebebrand, Manuel Focker, Judith Alferink
Summary: This study found altered phenotype of circulating B cell subsets and their positive correlation with body composition parameters in adolescents with anorexia nervosa. The research suggests that regulatory B cell subgroups are reduced in anorexia nervosa patients, potentially impacting the pathogenesis of the disorder.
SCIENTIFIC REPORTS
(2021)
Review
Cell Biology
Rafael Leite Dantas, Jana Freff, Oliver Ambree, Eva C. Beins, Andreas J. Forstner, Udo Dannlowski, Bernhard T. Baune, Stefanie Scheu, Judith Alferink
Summary: Dendritic cells (DCs), a heterogeneous population of myeloid cells, are believed to play a role in peripheral immune responses and neuroinflammation in mood disorders, although their specific mechanisms of involvement remain unclear. The review summarizes current knowledge on the role of DCs in mood disorders and discusses their impact on neuroinflammation and behavior in animal models of AD.
Review
Immunology
Marc Dalod, Stefanie Scheu
Summary: DCs play a crucial role in antigen presentation and orchestrate information transfer in the immune system. Mouse models provide valuable insights into the different functions of DC types and their activation states. This article provides comprehensive information on selecting mutant mouse strains for DC research and discusses the advantages, limitations, and future perspectives of these models.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Oncology
Jessica M. Salmon, Izabela Todorovski, Kym L. Stanley, Claudia Bruedigam, Conor J. Kearney, Luciano G. Martelotto, Fernando Rossello, Timothy Semple, Gisela Mir Arnau, Magnus Zethoven, Michael Bots, Stefan Bjelosevic, Leonie A. Cluse, Peter J. Fraser, Veronique Litalien, Eva Vidacs, Kate McArthur, Antony Y. Matthews, Elise Gressier, Nicole A. de Weerd, Jens Lichte, Madison J. Kelly, Simon J. Hogg, Paul J. Hertzog, Lev M. Kats, Stephin J. Vervoort, Daniel D. De Carvalho, Stefanie Scheu, Sammy Bedoui, Benjamin T. Kile, Steven W. Lane, Andrew C. Perkins, Andrew H. Wei, Pilar M. Dominguez, Ricky W. Johnstone
Summary: Inhibition of HDACs can induce differentiation and therapeutic effects in AML cells, and epigenetic rewiring of pDCs enhances antitumor immunity, offering a new therapeutic approach.
Article
Immunology
Xiaoli Yang, Shafaqat Ali, Manman Zhao, Lisa Richter, Vanessa Schaefer, Julian Schliehe-Diecks, Marian Frank, Jing Qi, Pia-Katharina Larsen, Jennifer Skerra, Heba Islam, Thorsten Wachtmeister, Christina Alter, Anfei Huang, Sanil Bhatia, Karl Koehrer, Carsten Kirschning, Heike Weighardt, Ulrich Kalinke, Rainer Kalscheuer, Markus Uhrberg, Stefanie Scheu
Summary: This study reveals that BEA activates BMDCs and induces the production of inflammatory cytokines and chemokines, which is dependent on the MyD88 and TRIF signaling pathways as well as TLR4. These findings contribute to a better understanding of the mechanism of action of BEA in immune cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Clinical Neurology
Jana Freff, Eva C. Beins, Lisa Broeker, Kathrin Schwarte, Rafael Leite Dantas, Carlo Maj, Volker Arolt, Udo Dannlowski, Markus M. Noethen, Bernhard T. Baune, Andreas J. Forstner, Judith Alferink
Summary: This study characterizes the expression pattern of CCR4 and its ligands CCL17 and CCL22 in major depressive disorder (MDD) and explores their clinical relevance in predicting disease severity. The results show that decreased CCR4 expression and increased CCL17/CCL22 levels are associated with MDD. CCR4 expression on CD4(+) T cells is correlated with depression severity and specific symptoms. Additionally, CCR4 and polygenic risk score (PRS) have predictive capacity for disease severity.
JOURNAL OF AFFECTIVE DISORDERS
(2022)
Article
Immunology
Manfred B. Lutz, Shafaqat Ali, Cindy Audiger, Stella E. Autenrieth, Luciana Berod, Venetia Bigley, Laura Cyran, Marc Dalod, Jan Doerrie, Diana Dudziak, Georgina Florez-Grau, Lucila Giusiano, Gloria J. Godoy, Marion Heuer, Anne B. Krug, Christian H. K. Lehmann, Christian T. Mayer, Shalin H. Naik, Stefanie Scheu, Gerty Schreibelt, Elodie Segura, Kristin Sere, Tim Sparwasser, Jurjen Tel, Huaming Xu, Martin Zenke
Summary: This article is part of a series of guidelines on dendritic cells, providing advanced protocols for the preparation and characterization of murine and human dendritic cells. The protocols cover the preparation and generation of dendritic cells from different cellular sources, including the generation of different subsets such as cDC1, cDC2, and pDC using Flt3L in mice, and the generation of bone marrow-derived dendritic cells using GM-CSF. For human dendritic cells, the protocols focus on the culture of CD34(+) cells on OP9 cell layers to generate different subsets, as well as the generation of dendritic cells from peripheral blood monocytes. The article has been peer-reviewed and approved by the co-authors, making it an essential resource for basic and clinical dendritic cell immunologists.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Meeting Abstract
Immunology
Xiaoli Yang, Lisa Richter, Stefanie Scheu
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Meeting Abstract
Immunology
Shafaqat Ali, Ritu Mann Nuettel, Regine Dress, Patrick Petzsch, Karl Ehrich Koehrer, Haifeng Chris Xu, Philipp Lang, Judith Alferink, Stefanie Scheu
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Meeting Abstract
Immunology
Marcel Marson, Christina Ruland, Judith Alferink, Stefanie Scheu
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Article
Genetics & Heredity
Ritu Mann-Nuettel, Shafaqat Ali, Patrick Petzsch, Karl Koehrer, Judith Alferink, Stefanie Scheu
Summary: This study reveals the role of transcription factors (TFs) in immune responses, particularly the less noticed but potentially important AP-1 family, in pDCs. The research demonstrates the involvement of TLR9-induced TFs in epigenetic modulation, NF kappa B and JAK-STAT signaling, and protein production in the endoplasmic reticulum.