Article
Infectious Diseases
Konstantina Gartzonika, Lida Politi, Angeliki Mavroidi, Andreas G. Tsantes, Nicholas Spanakis, Efthalia Priavali, Georgia Vrioni, Athanasios Tsakris
Summary: This study describes the epidemiological and molecular characteristics of a large-scale outbreak of NDM-1-producing Enterobacter cloacae complex in Greece, which is the largest clonal outbreak of its kind in Europe. The presence of NDM-1 gene was confirmed in the isolates and it was found to be associated with other resistance genes through genetic sequencing.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Infectious Diseases
Cecile Emeraud, Caroline Petit, Lauraine Gauthier, Remy A. Bonnin, Thierry Naas, Laurent Dortet
Summary: This study genetically characterized VIM-producing Enterobacter cloacae complex (ECC) isolates recovered in France from 2015 to 2018. The prevalence of ECC among VIM-producing Enterobacterales increased significantly during this period. Various species and sequence types were identified among these isolates. Additionally, different integrons carrying specific bla(VIM) variants were identified.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Pharmacology & Pharmacy
Qiuxia Lin, Menglu Wu, Hanbing Yu, Xiaojiong Jia, Hua Zou, Deyu Ma, Siqiang Niu, Shifeng Huang
Summary: In Chongqing, China, the study revealed that CRE infections were associated with high mortality rates and poor outcomes, with CPE patients more likely to exhibit antibiotic resistance. Independent predictors for CPE infection were ICU admission and hepatobiliary system diseases. Although there was no significant difference in desirability of outcome ranking (DOOR) outcomes between the two groups, CPE patients had prolonged ICU stays.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Infectious Diseases
Shixing Liu, Na Huang, Cui Zhou, Yishuai Lin, Ying Zhang, Lingbo Wang, Xiangkuo Zheng, Tieli Zhou, Zhongyong Wang
Summary: The study revealed that carbapenem-nonsusceptible ECC strains producing non-carbapenemase were predominant, and their resistance mechanisms were clarified. In addition, the overexpression of efflux pump was also associated with carbapenem resistance. The MLST results demonstrated the genetic diversity of ECC strains.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Microbiology
Zhile Xiong, Chao Zhang, Kurosh Sarbandi, Zhuwei Liang, Jialiang Mai, Bingshao Liang, Hao Cai, Xiantang Chen, Fei Gao, Fangjun Lan, Xiaochun Liu, Shuyan Liu, Zhenwen Zhou
Summary: Carbapenem-resistant Enterobacteriaceae (CRE) colonization in pediatric inpatients in Guangzhou, China was evaluated in this study. The molecular and clinical characteristics of CRE colonization were described, and risk factors for fecal CRE colonization were identified. The study highlights the importance of implementing infection control and prevention measures to reduce CRE colonization.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Shengnan Hu, Wenyan Xie, Qiwen Cheng, Xiaoning Zhang, Xiutao Dong, Huaiqi Jing, Jiazheng Wang
Summary: This study investigated the prevalence and characteristics of carbapenem-resistant Enterobacter cloacae complex (CREC) isolates in a tertiary hospital in Shandong, China. The most common carbapenemases detected were NDM-5 and NDM-1. These findings provide valuable information for infection control and treatment in hospitals.
Article
Infectious Diseases
Yoshiro Sakai, Kenji Gotoh, Ryuichi Nakano, Jun Iwahashi, Miho Miura, Rie Horita, Naoki Miyamoto, Hisakazu Yano, Mikinori Kannae, Osamu Takasu, Hiroshi Watanabe
Summary: The study evaluated a CRE outbreak in an ICU and found that various infection control measures, including partial closure, isolation, and division of staff, were essential in significantly reducing new cases, although it took several months to completely eradicate the outbreak.
Article
Infectious Diseases
Pamela Barbadoro, Daniela Bencardino, Elisa Carloni, Enrica Omiccioli, Elisa Ponzio, Rebecca Micheletti, Giorgia Acquaviva, Aurora Luciani, Annamaria Masucci, Antonella Pocognoli, Francesca Orecchioni, Marcello Mario D'Errico, Mauro Magnani, Francesca Andreoni
Summary: This study found a high prevalence of CPE in patients upon their admission, with over 50% of the strains being multidrug-resistant. The most prevalent plasmid profile was FIIK, FIB KQ (33%) and the most common sequence type was ST512.
Article
Microbiology
Chao-Ju Chen, Po-Liang Lu, Shu-Huei Jian, Hsiao-Ling Fu, Po-Hao Huang, Chung-Yu Chang
Summary: The Enterobacter cloacae complex (ECC) has emerged as a significant cause of nosocomial infections, especially with carbapenem-nonsusceptible isolates posing a threat to public health. This study identified the most commonly isolated species was E. hormaechei subsp. hoffmannii, with different species of CnsECC showing heterogeneity in resistance patterns. Carbapenemase-producing CnsECC infections were associated with poorer outcomes, with risk factors including exposure to carbapenems, chronic pulmonary disease, and ventilator support. The findings emphasize the importance of understanding resistance mechanisms to guide antibiotic treatment decisions.
Editorial Material
Biochemistry & Molecular Biology
Zhiyong Zong, Yu Feng, Alan McNally
Summary: Enterobacter is a globally important pathogen with resistance to carbapenem and colistin. Further research is needed to understand colistin heteroresistance and the emergence of certain Enterobacter lineages clinically.
TRENDS IN MICROBIOLOGY
(2021)
Article
Microbiology
Cristina Lasarte-Monterrubio, Paula Guijarro-Sanchez, Juan Carlos Vazquez-Ucha, Isaac Alonso-Garcia, Laura Alvarez-Fraga, Michelle Outeda, Marta Martinez-Guitian, Andrea Pena-Escolano, Romina Maceiras, Emilio Lence, Concepcion Gonzalez-Bello, Jorge Arca-Suarez, German Bou, Alejandro Beceiro
Summary: Resistance to cefiderocol has emerged among carbapenemase-producing Enterobacterales, particularly in the Enterobacter cloacae complex. This resistance is mainly due to the production of VIM-1 carbapenemase and may also involve the co-production of SHV-12 and inactivation of the FcuA-like siderophore receptor.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Microbiology
Satomi Takei, Yu Jie Lu, Mari Tohya, Shin Watanabe, Shigeki Misawa, Yoko Tabe, Takashi Miida, San Mya, Htay Htay Tin, Tatsuya Tada, Teruo Kirikae
Summary: This study demonstrates the spread of carbapenem-resistant K. pneumoniae strains in medical settings in Myanmar. Specifically, plasmid genes encoding NDMs and 16S rRNA methylases have been spreading in high-risk clones of K. pneumoniae.
MICROBIOLOGY SPECTRUM
(2022)
Article
Environmental Sciences
Cai-Hong Guo, Yu-Qing Liu, Yan Li, Xiao-Xiao Duan, Ting-Yu Yang, Fang-Yu Li, Ming Zou, Bao-Tao Liu
Summary: The widespread presence of carbapenem-resistant Enterobacteriaceae (CRE) and mcr-positive Escherichia coli (MCREC) in large-scale rivers in China poses a significant threat to animal and human health. A study conducted in Shandong Province sampled 86 rivers and found a prevalence of 16.3% for CRE and 27.9% for MCREC. Multiple antibiotic-resistant isolates were obtained, including those carrying blaKPC-2, blaNDM, and mcr-1 genes.
ENVIRONMENTAL POLLUTION
(2023)
Article
Microbiology
Ryuichiro Abe, Yukihiro Akeda, Yo Sugawara, Yuki Matsumoto, Daisuke Motooka, Ryuji Kawahara, Norihisa Yamamoto, Kazunori Tomono, Tetsuya Iida, Shigeyuki Hamada
Summary: The global dissemination of carbapenem-resistant Enterobacteriaceae (CRE), along with carbapenem heteroresistance, poses a major threat to human health by limiting antibiotic treatment options. Mechanisms such as porin mutation and tandem amplification of carbapenemase genes contribute to enhanced carbapenem resistance, potentially leading to treatment failure in clinical settings.
Article
Infectious Diseases
Mao Hagihara, Hideo Kato, Toshie Sugano, Hayato Okade, Nobuo Sato, Yuichi Shibata, Daisuke Sakanashi, Jun Hirai, Nobuhiro Asai, Hiroyuki Suematsu, Yuka Yamagishi, Hiroshige Mikamo
Summary: The study found that combination therapies with beta-lactams (aztreonam, cefepime, and meropenem) and nacubactam showed enhanced antimicrobial activity against CRE Enterobacter cloacae and Klebsiella pneumoniae. Most combination therapies exhibited bacteriostatic to bactericidal activities against CRE isolates.
Article
Microbiology
Masataka Goto, Akio Abe, Tomoko Hanawa, Masato Suzuki, Asaomi Kuwae
Summary: In this study, we discovered that a chaperone-like protein called Bcr4 interacts with BscI, a component of the type III secretion system (T3SS) machinery in Bordetella bronchiseptica. Our results showed that a B. bronchiseptica strain lacking BscI was unable to secrete type III secreted proteins. Furthermore, in a strain that overproduces T3SS component proteins, Bcr4 is required to maintain the stability of BscI in bacterial cells. These findings suggest that Bcr4 plays a crucial role in stabilizing BscI and supporting the construction of the T3SS machinery.
MICROBIOLOGY SPECTRUM
(2022)
Article
Veterinary Sciences
Phuong Thi Lan Nguyen, Thi Hong Hanh Ngo, Thi Mai Hung Tran, Thi Ngoc Bich Vu, Viet Thanh Le, Hai Anh Tran, Duy Thai Pham, Ha Thanh Nguyen, Dieu Linh Tran, Thi Phuong Lien Nguyen, Thi Thi Tho Nguyen, Nhu Duong Tran, Duc Anh Dang, Anne-Laure Banuls, Marc Choisy, H. Rogier van Doorn, Masato Suzuki, Huy Hoang Tran
Summary: Livestock in Vietnam harbors a diverse range of MCRPEC strains with various sequence types and multiple antimicrobial resistance (AMR) genes, mainly spread through plasmids rather than clonal dissemination of MCRPEC strains.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Microbiology
Ryuichiro Abe, Yukihiro Akeda, Noriko Sakamoto, Dan Takeuchi, Yo Sugawara, Norihisa Yamamoto, Anusak Kerdsin, Yuki Matsumoto, Daisuke Motooka, Warawut Laolerd, Pitak Santanirand, Masato Suzuki, Keigo Shibayama, Kazunori Tomono, Tetsuya Iida, Shigeyuki Hamada
Summary: The study revealed a high prevalence of Enterobacteriaceae isolates carrying bla(NDM-1) and bla(NDM-5) in clinical samples from Thailand. The dissemination of carbapenem-resistant Enterobacteriaceae (CRE) in Thailand was found to be mainly due to clonal spread of specific E. coli and Klebsiella pneumoniae clones carrying bla(NDM) genes.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Infectious Diseases
Tomomi Hishinuma, Tatsuya Tada, Mari Tohya, Masaki Shintani, Masato Suzuki, Masahiro Shimojima, Teruo Kirikae
Summary: The study aimed to investigate the significance of a tandem duplicate of bla(VIM-24) in Pseudomonas aeruginosa ST1816 isolates. Thirteen isolates carrying bla(VIMs) were collected from medical settings in Japan, and four of them had a tandem duplicate of bla(VIM-24). These plasmids conferred resistance to cefepime and transferred to PAO1. Western blotting analysis showed higher production of VIM-24 metallo-beta-lactamase in strains with the tandem duplicate. This suggests that the tandem duplicate of bla(VIM-24) contributes to the proliferation of ST1816 strains in Japanese hospitals.
MICROBIAL DRUG RESISTANCE
(2023)
Article
Microbiology
Samiratu Mahazu, Isaac Prah, Yusuke Ota, Takaya Hayashi, Yoko Nukui, Masato Suzuki, Yoshihiko Hoshino, Yukihiro Akeda, Toshihiko Suzuki, Tomoko Ishino, Anthony Ablordey, Ryoichi Saito
Summary: IncX3 and IncL plasmids play important roles in the dissemination of antibiotic resistance genes. Clinical samples from a Ghanaian hospital were used to investigate the genetic characteristics of bla(OXA-48)- and bla(OXA-181)-containing Klebsiella pneumoniae, Klebsiella quasipneumoniae, and Enterobacter cloacae. The study found that these plasmids were highly transferable and enhanced the competitive ability of host cells.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Yusuke Hashimoto, Masato Suzuki, Sae Kobayashi, Yuki Hirahara, Jun Kurushima, Hidetada Hirakawa, Takahiro Nomura, Koichi Tanimoto, Haruyoshi Tomita
Summary: Linear plasmids with high self-transmissibility and the ability to acquire and maintain antimicrobial resistance (AMR) genes, particularly through transposition with IS1216E, have been identified in clinical multidrug-resistant enterococci. These plasmids play a crucial role in the spread and maintenance of AMR genes among enterococci.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Microbiology
Masaki Shintani, Haruo Suzuki, Hideaki Nojiri, Masato Suzuki
Summary: This study reevaluates previously published data based on the article titled 'IncP-type plasmids carrying genes for antibiotic resistance or aromatic compound degradation are prevalent in sequenced Aromatoleum and Thauera strains' by Lo et al. This correspondence aims to clarify misconceptions about plasmids classified under incompatibility (Inc) groups IncP-1 and IncP-11.
ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Microbiology
Noriko Sakamoto, Warawut Laolerd, Yukihiro Akeda, Yo Sugawara, Daisuke Motooka, Norihisa Yamamoto, Dan Takeuchi, Rathina Kumar Shanmugakani, Isao Nishi, Masato Suzuki, Keigo Shibayama, Tetsuya Iida, Pitak Santanirand, Kazunori Tomono, Shigeyuki Hamada
Summary: This study analyzed the whole-genome sequencing data of carbapenemase-producing Klebsiella pneumoniae (CPKP) isolated from a tertiary-care hospital in Bangkok, Thailand. The findings revealed the molecular epidemiology of CPKP in terms of genetic, sequence type, and phylogenetic relationships. The study identified a temporal shift in the predominant carbapenemase genes and the emergence of CPKP co-harboring multiple carbapenemase genes, indicating significant changes in CPE types in Thailand and potentially in Southeast Asian countries.
JOURNAL OF MEDICAL MICROBIOLOGY
(2023)
Article
Infectious Diseases
Hoang Thi An Ha, Phuong Thi Lan Nguyen, Tran Thi Mai Hung, Le Anh Tuan, Bui Thanh Thuy, Tran Hoang My Lien, Pham Duy Thai, Nguyen Ha Thanh, Vu Thi Ngoc Bich, Tran Hai Anh, Ngo Thi Hong Hanh, Nguyen Thi Minh, Duy Pham Thanh, Si-Nguyen T. Mai, Hao Chung The, Nguyen Vu Trung, Nguyen Hoai Thu, Tran Nhu Duong, Dang Duc Anh, Pham Thi Ngoc, Anne-Laure Banuls, Marc Choisy, H. Rogier van Doorn, Masato Suzuki, Tran Huy Hoang
Summary: This study investigates the prevalence of optrA positive E. faecalis (OPEfs) in 6 reservoirs in farms in Ha Nam province, Vietnam, and its associated factors, as well as explores the genetic relationship of OPEfs isolates. The highest prevalence of OPEfs was found in flies (46.8%), followed by chickens (37.3%), dogs (33.3%), humans (18.7%), wastewater (16.4%), and pigs (11.3%). The presence of OPEfs was associated with the total feeding area and total livestock unit of the farm in chickens, flies, and wastewater. Moreover, 86% of OPEfs strains were resistant to the last resort antibiotic linezolid.
Article
Multidisciplinary Sciences
Hidenori Matsu, Emiko Rimbara, Masato Suzuki, Kengo Tokunaga, Hidekazu Suzuki, Masaya Sano, Takashi Ueda, Hitoshi Tsugawa, Sohachi Nanjo, Akira Takeda, Makot Sakshi, Shuich Terao, Tsuyosh Suda, Sae Aoki, Keigo Shibayama, Hiroyosh Ota, Katsuhiro Mabe
Summary: Helicobacter suis, the most common gastric non-Helicobacter pylori Helicobacter species found in humans, has been suggested to cause many cases of gastric disease, but the transmission route from hogs remains unclear. Diagnostic methods for H. suis infection often yield negative results, making it difficult to diagnose without gastric biopsy specimens. This study introduces the use of whole-bacterial cell ELISA to simultaneously assess H. suis and H. pylori infections, with high accuracy and sensitivity.
Letter
Dermatology
Ryo Fukaura, Chiaki Terashima-Murase, Mai Ota, Haruka Noda, Keisuke Oka, Yuka Ishihara, Keigo Shibayama, Masashi Akiyama
JOURNAL OF DERMATOLOGY
(2023)
Letter
Immunology
Masaya Sano, Emiko Rimbara, Masato Suzuki, Hidenori Matsui, Miwa Hirai, Sae Aoki, Tsuyoshi Kenri, Keigo Shibayama, Hidekazu Suzuki
EMERGING INFECTIOUS DISEASES
(2023)
Article
Veterinary Sciences
Md Amirul Hasan, Masato Suzuki, Kouji Sakai, Md Humayun Kabir, Yu Miyaoka, Hakimullah Hakim, Chisaki Kadota, Dany Shoham, Kazuaki Takehara
Summary: Full genome sequencing of two bovine RVA strains isolated in Japan in 2019 revealed rare genotype constellations and suggested their evolution through multiple reassortment events from Japanese cattle RVA strains. The emergence of these RVA strains and their reassortment with locally circulating atypical RVAs could have implications for current vaccination strategies.
JOURNAL OF VETERINARY MEDICAL SCIENCE
(2023)
Article
Biochemical Research Methods
Hidenori Matsui, Masato Suzuki, Sae Aoki, Keigo Shibayama, Kengo Tokunaga, Hidekazu Suzuki, Katsuhiro Mabe, Tsuyoshi Kenri, Emiko Rimbara
Summary: This article presents a protocol for detecting Helicobacter suis infection, including sample collection, DNA preparation, PCR, bacterial culture, and whole-genome sequencing.
Article
Medicine, Research & Experimental
Yuki Hara, Mitsutaka Iguchi, Nobuyuki Tetsuka, Hiroshi Morioka, Aki Hirabayashi, Masato Suzuki, Yuka Tomita, Keisuke Oka, Tetsuya Yagi
Summary: Carbapenemase-producing Enterobacterales (CPE) have raised concerns about infectious disease treatment options and infection control. A multicenter study conducted in Aichi Prefecture from 2015 to 2019 revealed the molecular epidemiology of CPE. The study found that CPE incidence was 0.12%, with Klebsiella pneumoniae and Enterobacter cloacae complex as the main species. The predominance of ST78 in E. cloacae complex and the spread of ST517 and ST592 in carbapenemase-producing K. pneumoniae indicate the need for strengthened infection control programs.
NAGOYA JOURNAL OF MEDICAL SCIENCE
(2022)
