Article
Engineering, Biomedical
Cheng Chang, Wennan Guo, Xinbo Yu, Chaoyi Guo, Nan Zhou, Xiaokui Guo, Ru-Lin Huang, Qingtian Li, Yongzhang Zhu
Summary: Bacteriophages, particularly M13 phages, have been extensively studied in the field of nanomedicine due to their unique biological advantages and engineering capabilities. The nanofiber-like morphology of M13 phages allows them to reach various target sites and self-assemble into multidimensional scaffolds. Genetic modifications enable the display of peptides and antibodies on the phages, making them valuable for precise and individualized medicine. Researchers have also used novel engineering approaches to enhance the bionanomaterial properties of M13 phages, leading to applications in drug delivery, biodetection, tissue regeneration, and targeted cancer therapy.
MATERIALS TODAY BIO
(2023)
Article
Biochemistry & Molecular Biology
Irum Rizvi, Malik Hisamuddin, Ajamaluddin Malik, Rizwan Hasan Khan
Summary: This study focused on host factors influencing the replication of Geminiviruses, identifying potential host proteins through phage display experiments and confirming the interaction of AtMCM3 with MYMIV Rep. The physiological relevance of AtMCM3 in MYMIV DNA replication was demonstrated, and yeast replication assay supported this interaction.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Nanoscience & Nanotechnology
Ni Meng, Xiaolong Cheng, Mengyao Sun, Yushan Zhang, Xueke Sun, Xifu Liu, Jing Chen
Summary: This study identified specific nanobodies targeting the monkeypox virus protein A35R, which can serve as potential candidates for the development of monkeypox vaccines and diagnostic kits. The nanobodies displayed high affinity and specificity for A35R, laying the foundation for further research and applications in diagnosing and treating monkeypox.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Engineering, Biomedical
Kshitiz Raj Shrestha, Do Hoon Lee, Woojae Chung, Seung-Wuk Lee, Byung Yang Lee, So Young Yoo
Summary: The engineered nanofibrous phage provides therapeutic cues for de novo soft tissue niche engineering, promoting the proper functioning of implanted stem cells and enhancing blood vessel regeneration.
Article
Biochemistry & Molecular Biology
Jiahang Wu, Taijun Chen, Yingying Wang, Jiafan Bai, Chenwen Lao, Minyue Luo, Mingxia Chen, Wenzhen Peng, Wei Zhi, Jie Weng, Jianxin Wang
Summary: This study aimed to promote tissue regeneration by designing a biomimetic hydrogel with piezoelectric and antibacterial properties through the incorporation of piezoelectric materials and silver nanowires. The results showed enhanced repair effect of osteochondral defects, indicating the potential application of bionics principle- and piezoelectric effect-based osteochondral tissue engineering.
Review
Chemistry, Multidisciplinary
Xinyu Song, Ling Xu, Wenjie Zhang
Summary: Critical-size bone defect repair is challenging, and current therapies have limitations. Extracellular vesicle (EV)-based strategies offer a promising approach for tissue regeneration due to their complex composition and low immunogenicity. Optimizing the synthesis of biomimetic EVs can enhance their bioactivity and improve bone regeneration. Integrating nanoparticles into bone graft biomaterials can maximize the therapeutic efficacy of EVs. This review summarizes the role of EVs in bone regeneration, discusses strategies for optimizing biomimetic synthetic EVs, and explores the use of bone graft biomaterials for EV delivery to repair bone defects.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Multidisciplinary Sciences
Makoto Nakakido, Naoki Tanaka, Ayako Shimojo, Nobuhiro Miyamae, Kouhei Tsumoto
Summary: Respiratory infectious diseases pose a serious global threat, and novel antiviral materials are in high demand. In this study, a new platform was constructed to evaluate antiviral activity of surface coating materials using the M13 bacteriophage and phagemid system. The experimental system developed in this study provides rapid and effective screening, which can be applied to the development of novel antiviral materials.
Article
Engineering, Biomedical
Zhuo Li, Haixing Wang, Kunyu Zhang, Boguang Yang, Xian Xie, Zhengmeng Yang, Lingchi Kong, Peng Shi, Yuan Zhang, Yi-Ping Ho, Zhi-Yong Zhang, Gang Li, Liming Bian
Summary: The research assembled a biomimetic hyaluronic acid nanocomposite hydrogel with bisphosphonates (BPs), and demonstrated its expedited release of BPs in an acidic environment. The in vitro studies showed that the hydrogel inhibits mature osteoclastic differentiation, while promoting the initial differentiation of primary macrophages to preosteoclasts. In vivo evaluation confirmed the hydrogel's ability to enhance in situ bone regeneration.
BIOACTIVE MATERIALS
(2022)
Review
Biotechnology & Applied Microbiology
Feng Han, Qingchen Meng, En Xie, Kexin Li, Jie Hu, Qianglong Chen, Jiaying Li, Fengxuan Han
Summary: The incidence of tissue and organ damage caused by various diseases is increasing worldwide. Tissue engineering, using biomaterials with specific structures, has the potential to regenerate or replace damaged tissues and organs. Micro/nano materials, with their ability to mimic the microstructure of tissues, have greatly advanced the field of tissue engineering. This review provides an overview of different types of micro/nanomaterials, their preparation methods and their applications in tissue regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Engineering, Biomedical
Chang Xie, Jinchun Ye, Renjie Liang, Xudong Yao, Xinyu Wu, Yiwen Koh, Wei Wei, Xianzhu Zhang, Hongwei Ouyang
Summary: This review focuses on strategies of engineered grafts for bone regeneration, emphasizing physicochemical properties and structure optimization to enhance bone regeneration, as well as biological strategies to increase repair efficiency.
ADVANCED HEALTHCARE MATERIALS
(2021)
Review
Chemistry, Analytical
Felicia Carotenuto, Sara Politi, Arsalan Ul Haq, Fabio De Matteis, Emanuela Tamburri, Maria Letizia Terranova, Laura Teodori, Alessandra Pasquo, Paolo Di Nardo
Summary: The failure of tissues and organs due to degenerative diseases or trauma is a major concern globally. Tissue engineering offers a potential solution by regenerating or replacing damaged tissues and organs. Biomaterials play a crucial role in promoting new tissue formation, with their architectural features influencing cellular behaviors. Optimizing the micro/nano architecture of biomaterials is essential for successful regenerative therapy. Various micro/nanostructured biomaterials have been developed to mimic the structure of different soft and hard tissues, with a focus on muscle, cartilage, and bone tissue regeneration.
Article
Chemistry, Multidisciplinary
Laura Franz, Jesko Koehnke
Summary: This study demonstrates a Sortase A-based approach for modifying peptides with enzymes from unrelated pathways, leading to the synthesis of a new-to-nature, hybrid ribosomal natural product in a one-pot reaction.
CHEMICAL COMMUNICATIONS
(2021)
Review
Engineering, Multidisciplinary
Lvxing Huang, Le Chen, Hengyi Chen, Manju Wang, Letian Jin, Shenghai Zhou, Lexin Gao, Ruwei Li, Quan Li, Hanchang Wang, Can Zhang, Junjuan Wang
Summary: Tendon tissue is important for muscle-to-bone connection and stress transfer, but its complex structure and poor self-healing capacity make tendon injury a significant clinical challenge. Advances in technology have led to the development of treatments using biomaterials, growth factors, and stem cells, which mimic the extracellular matrix of tendon tissue for improved repair and regeneration. This review discusses the constituents and structural features of tendon tissue, biomimetic scaffolds for tendon tissue engineering, as well as novel strategies and challenges in tendon regeneration and repair.
Article
Chemistry, Multidisciplinary
Yanhuizhi Feng, Huai-Ling Gao, Di Wu, Yu-Teng Weng, Ze-Yu Wang, Shu-Hong Yu, Zuolin Wang
Summary: The bio-inspired lamellar chitosan scaffold (LCS), manufactured through a bidirectional freezing method, shows excellent mechanical properties in the hydrated state and accelerates vessel formation and soft tissue regeneration in vivo, as well as inducing macrophage differentiation to M2 macrophages.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Review
Microbiology
Su Jin Jo, Jun Kwon, Sang Guen Kim, Seung-Jun Lee
Summary: Bacterial infections have become a global threat, and bacteriophages are gaining attention as promising alternatives due to their distinctive attributes and genetic modification abilities. Phage engineering can enhance diagnostics, drug delivery, and therapy, and holds promise in transforming precision medicine, addressing antibiotic resistance, and advancing diverse applications.
Article
Engineering, Biomedical
Ganchimeg Davaa, Jin Young Hong, Jung-Hwan Lee, Min Soo Kim, Jennifer O. Buitrago, Yu-Meng Li, Hae-Hyoung Lee, Dong Wook Han, Kam W. Leong, Jung Keun Hyun, Hae-Won Kim
Summary: Neural stem cells (NSC) have the potential to regenerate injured neural tissues, but suffer from low survival and uncontrolled differentiation. This study demonstrates that mechanically-tuned protein hydrogels can protect and enhance the long-term survival of reprogrammed NSC, alleviate neural inflammation, promote differentiation, and improve locomotor functions in treating injured spinal cord.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Seon Yeong Chae, Sangheon Jeon, Dong-Wook Han, Suck Won Hong
Summary: Researchers have investigated the excellent antibacterial activity of graphene-based materials, which utilize the unique mechanical, electrical, and surface properties of graphene, to address the challenges of bacterial drug resistance and emerging infectious diseases. The antibacterial mechanism involves physical damage and chemical interactions between graphene and bacterial membranes. A simple manufacturing process was also developed to create three-dimensionally arranged graphene oxide films, which exhibited antibacterial activity.
ENVIRONMENTAL SCIENCE-NANO
(2023)
Review
Biochemistry & Molecular Biology
Moon Sung Kang, Jinju Jang, Hyo Jung Jo, Won-Hyeon Kim, Bongju Kim, Heoung-Jae Chun, Dohyung Lim, Dong-Wook Han
Summary: This review focuses on the advances and innovations of 3D bioprinted skin for skin regeneration. By improving materials, structures, and functions, the clinical potential of 3D bioprinted skin has been enhanced. With state-of-the-art printing technology and novel biomaterials, highly developed skin comprising stratified epidermis, dermis, and hypodermis with blood vessels, nerves, muscles, and skin appendages can be fabricated. We hope that this review will guide future research and clinical applications.
Article
Nanoscience & Nanotechnology
Sangmun Choi, Iruthayapandi Selestin Raja, Aravindha Raja Selvaraj, Moon Sung Kang, Tae-Eon Park, Ki Su Kim, Suong-Hyu Hyon, Dong-Wook Han, Jong-Chul Park
Summary: Carbon nanotubes are commonly used one-dimensional carbonaceous nanomaterials in tissue engineering, while other forms of one-dimensional nanomaterials, such as carbon nanowires and carbon nanofibers, have not been extensively explored for biomedical applications. In this study, activated carbon nanofiber nanoparticles (ACNF NPs) were synthesized from polyacrylonitrile electrospun nanofibers. Two different types of ACNF NPs-containing electrospun polycaprolactone (PCL) nanofiber mats were prepared and evaluated for their potential as skin tissue engineering scaffolds. The results showed that ACNF NPs exhibited a graphitization degree with an I-D/I-G ratio of 1.05, and had a diameter of 280 +/- 100 nm and a length of 565-3322 nm. NPs with a concentration above 30 μg/mL exhibited toxicity to NIH3T3 fibroblasts. The ACNF-PCL nanofiber mats demonstrated better cell proliferation compared to PCL and ACNF@PCL mats. Therefore, it can be concluded that ACNF-PCL mats with a lower concentration of ACNF NPs have greater potential to support cellular growth and impact skin tissue regeneration.
ADVANCED COMPOSITES AND HYBRID MATERIALS
(2023)
Review
Engineering, Biomedical
Iruthayapandi Selestin Raja, Moon Sung Kang, Suck Won Hong, Hojae Bae, Bongju Kim, Yu-Shik Hwang, Jae Min Cha, Dong-Wook Han
Summary: Biofabrication approaches, such as 3D bioprinting of hydrogels, have gained increasing attention for their ability to construct complex 3D tissue and organ structures. However, the stability and shape fidelity of printed gels can be affected by various factors. To address this, researchers have incorporated different nanomaterials into polymeric hydrogels. This review discusses CFNs-containing printable gels in various biomedical applications, including the types of bioprinters, prerequisites of bioink and biomaterial ink, and the progress and challenges in this field.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Polymer Science
Soloman Agnes Mary, Naisini Ariram, Arun Gopinath, Senthil Kumar Chinnaiyan, Iruthayapandi Selestin Raja, Bindia Sahu, Venkateshwarapuram Rengaswami Giri Dev, Dong-Wook Han, Balaraman Madhan
Summary: In this study, a fibrous mat incorporating the bioactive extract of Cassia auriculata (CA) with polycaprolactone (PCL) was developed using the centrifugal spinning technique. The concentration of PCL and the spinning speed were optimized to achieve better fiber formation. The developed fibrous mat showed fine pores and high biocompatibility for cell proliferation.
Article
Cell Biology
Moon Sung Kang, Rowoon Park, Hyo Jung Jo, Yong Cheol Shin, Chang-Seok Kim, Suong-Hyu Hyon, Suck Won Hong, Junghwan Oh, Dong-Wook Han
Summary: In recent years, bone tissue engineering has made significant progress in promoting the direct and functional connection between bone and graft to facilitate the healing of damaged bone tissues. This study introduces a new method for synthesizing reduced graphene oxide (rGO) and hydroxyapatite (HAp) using environmentally friendly materials. The synthesized E-rGO/HAp composites showed exceptional properties for use as bone tissue engineering scaffolds and promoted the proliferation and osteogenic differentiation of human mesenchymal stem cells. These composites have the potential to be used as BTE scaffolds, stem-cell differentiation stimulators, and implantable device components due to their biocompatible and bioactive properties.
Article
Biochemistry & Molecular Biology
Gyeonghyeon Kim, Dong-Wook Han, Jong Hun Lee
Summary: In this study, baicalein was found to have antioxidant effects and could protect HaCaT keratinocytes from H2O2-induced oxidative stress by maintaining mitochondrial homeostasis and cellular tight junctions.
Article
Multidisciplinary Sciences
Seok Hyun Lee, Moon Sung Kang, Sangheon Jeon, Hyo Jung Jo, Suck Won Hong, Bongju Kim, Dong-Wook Han
Summary: Contemporary advances in 3D bioprinting have allowed for the creation of personalized live 3D tissue mimetics. The development of bioink materials that accurately replicate the composition of native extracellular matrix and mimic cell properties has been emphasized. In this study, MXene-incorporated GelMA/HAMA bioinks were prepared to investigate their potential for spontaneous osteodifferentiation of human mesenchymal stem cells (hMSCs) when used for 3D printing. The GelMA/HAMA-MXene hydrogels were found to provide favorable matrices for hMSCs growth and survival, and hMSCs were shown to differentiate into osteoblasts in the GelMA-HAMA/MXene composites, creating a favorable microenvironment for bone regeneration. These findings suggest that MXene-incorporated GelMA/HAMA bioink has significant biofunctional advantages for the development of effective scaffolds in bone tissue regeneration.
Article
Biotechnology & Applied Microbiology
Xiaoting Ma, Won-Hyeon Kim, Jong-Ho Lee, Dong-Wook Han, Sung-Ho Lee, Jisung Kim, Dajung Lee, Bongju Kim, Dong-Myeong Shin
Summary: The study developed an air-barrier device to reduce bioaerosol contamination in dental environments. Through simulation and experiments, it was demonstrated that the air-barrier device effectively reduces the generation of bioaerosols in dental environments. This study shows that air-barrier devices play an effective role in reducing contaminating particles in dental environments.
BIOENGINEERING-BASEL
(2023)
Article
Chemistry, Multidisciplinary
Hee Jeong Jang, Moon Sung Kang, Won-Hyeon Kim, Hyo Jung Jo, Sung-Ho Lee, Eun Jeong Hahm, Jung Hyun Oh, Suck Won Hong, Bongju Kim, Dong-Wook Han
Summary: We used 3D printing to fabricate graphene oxide (GO)-incorporated polylactic acid (PLA) films and explored their benefits as barrier membranes in guided bone regeneration (GBR). Our results showed that the 3D printed GO-PLA films provided a favorable environment for preosteoblasts and promoted new bone formation in rat calvarial bone defect models.
NANOSCALE ADVANCES
(2023)
Article
Chemistry, Multidisciplinary
Anara Molkenova, Lazzat Serik, Alina Ramazanova, Kamila Zhumanova, Bakyt Duisenbayeva, Ainur Zhussupbekova, Kuanysh Zhussupbekov, Igor V. Shvets, Ki Su Kim, Dong-Wook Han, Timur Sh. Atabaev
Summary: Metal-doped carbon dots, specifically terbium-doped CDs, have been studied as a potential contrast agent for computed tomography. Physicochemical analysis showed that the prepared Tb-CDs have small sizes, high terbium concentration, and good colloidal stability. Preliminary tests also indicated that Tb-CDs have negligible cytotoxicity and demonstrate high X-ray absorption performance.
Article
Energy & Fuels
Dongkyu Choi, Hyuksang Kwon, Seonguk Lim, Taekyun Jeong, Heechul Jung, Yong-Il Kim, Dongwook Han
Summary: Ti-doped orthorhombic LiMn1-xTixO2 serves as a Co-and Ni-free cathode material for Li-ion rechargeable batteries with stable oxygen/cation site occupancy and cycling-dependent phase transition. The Ti doping improves the structural stability and reduces imperfections of the oxygen/cation lattices in LiMnO2. The electrochemically inactive cLiTiO2 phase suppresses the structural collapse of Ti-doped LiMnO2, resulting in higher discharge capacity and superior cycling performance compared to pristine LiMnO2.
