Article
Biochemistry & Molecular Biology
Le Tang, Wei Xiong, Lishen Zhang, Dan Wang, Yian Wang, Yingfen Wu, Fang Wei, Yongzhen Mo, Xiangchan Hou, Lei Shi, Fang Xiong, Shanshan Zhang, Zhaojian Gong, Qianjin Liao, Bo Xiang, Wenling Zhang, Ming Zhou, Xiaoling Li, Guiyuan Li, Can Guo, Zhaoyang Zeng
Summary: This study revealed that circSETD3 promotes invasion and migration of NPC cells by competitively adsorbing miR-615-5p and miR-1538, thus regulating the expression of MAPRE1. This suggests circSETD3 could be a potential molecular marker and therapeutic target for NPC.
Article
Medicine, Research & Experimental
Qi Li, Xiaoxia Liu, Weifang Liu, Yang Zhang, Mengying Wu, Zhirui Chen, Yin Zhao, Li Zou
Summary: In early-onset preeclampsia (EOPE), decreased expression of MALAT1 and PFKFB3 is associated with endothelial cell dysfunction. PFKFB3 modulates the proliferation, migration, and tube formation of ECs by regulating glycolysis, while MALAT1 regulates PFKFB3 expression by sponging miR-26a/26b. MALAT1 knockdown reduces EC angiogenesis by inhibiting PFKFB3-mediated glycolysis flux, suggesting that strategies targeting PFKFB3-driven glycolysis may be a promising approach for EOPE treatment.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Developmental Biology
Di Gao, Xin Wang, Ye-Lian Yan, Chao Li, Yong-Peng Tan, Qiu-Chen Liu, Meng-Ya Zhang, Jian Zhang, Qing-Yuan Sun, Zu-Bing Cao, Yun-Hai Zhang
Summary: circKDM5B regulates porcine early embryo development by mediating miR-128. Knockdown of circKDM5B leads to reduced blastocyst formation, decreased cell numbers, impaired tight junction assembly, and disturbed paracellular sealing within the TE epithelium. Injection of miR-128 inhibitor rescues the early development of circKDM5B knockdown embryos.
MOLECULAR HUMAN REPRODUCTION
(2023)
Article
Cell Biology
Yan Cui, Xintong Zhao, Yangang Wu
Summary: This study found that circ_0005918 is overexpressed in intervertebral disc degeneration (IDD) and promotes cell growth, ECM degradation, and secretion of inflammatory cytokines. Furthermore, circ_0005918 acts through sponging miR-622, and the expression level of miR-622 is correlated with the severity of IDD.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Oncology
Mei-Li Ma, Hong-Yan Zhang, Shu-Yi Zhang, Xiao-Li Yi
Summary: CDKN2B-AS1 is upregulated in colorectal cancer and plays a role in promoting CRC progression by modulating cell proliferation and apoptosis. It directly binds to miR-28-5p and functions as a ceRNA to regulate URGCP expression in CRC tissue. These findings suggest that CDKN2B-AS1 may serve as a potential therapeutic target or novel diagnostic biomarker for colorectal cancer.
Article
Cell Biology
Xingguang Liu, Tengda Zhao, Zhe Yuan, Shaohua Ge
Summary: This study found that lncRNA-MIR600HG plays a role in regulating glycometabolism and cisplatin resistance in oral squamous cell carcinoma cells by modulating the expression of miR-125a-5p and RNF44.
CELL DEATH DISCOVERY
(2022)
Article
Biochemistry & Molecular Biology
Yixuan Fan, Zhen Zhang, Kaiping Deng, Ziqi Kang, Jinjing Guo, Guomin Zhang, Yanli Zhang, Feng Wang
Summary: In this study, high-throughput sequencing technology and bioinformatics tools were used to study the expression patterns and alterations of circRNAs in the longissimus dorsi muscle of two developmental stages of goats. A total of 831 differently expressed circRNAs were identified, with 486 up-regulated circRNAs and 345 down-regulated circRNAs in kid skeletal muscles. The highly expressed circUBE3A was found to regulate the proliferation and differentiation of goat myoblasts by binding and inhibiting miR-28-5p.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Di Sun, Jiaqi An, Zixu Cui, Jiao Li, Ziwei You, Chang Lu, Yang Yang, Pengfei Gao, Xiaohong Guo, Bugao Li, Chunbo Cai, Guoqing Cao
Summary: This study identifies a novel circRNA, circCSDE1, that is highly expressed in skeletal muscle. Functional studies show that circCSDE1 promotes proliferation and inhibits myoblast differentiation, while miR-21-3p has opposite effects. Dual-luciferase reporter assay confirms that circCSDE1 targets miR-21-3p directly.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Zhen Zhang, Yixuan Fan, Kaiping Deng, Yaxu Liang, Guomin Zhang, Xiaoxiao Gao, M. A. El-Samahy, Yanli Zhang, Mingtian Deng, Feng Wang
Summary: In this study, the function of circUSP13 in goat myogenic differentiation was investigated. The results showed that circUSP13 promotes differentiation and inhibits apoptosis in goat primary myoblasts by interacting with miR-29c and regulating the expression of IGF1. This study identifies circUSP13 as a potential molecular marker for breeding programs and suggests the circUSP13-miR-29c-IGF1 axis as a therapeutic target for combating muscle wasting.
Article
Biochemistry & Molecular Biology
Yulin He, Peiyu Yang, Tiantian Yuan, Lin Zhang, Gongshe Yang, Jianjun Jin, Taiyong Yu
Summary: This study reveals that miR-103-3p promotes proliferation and inhibits differentiation in C2C12 myoblasts by targeting BTG2.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Qin Zhu, Xian Zhang, Hong-Yan Zai, Wei Jiang, Ke-Jing Zhang, Yu-Qiong He, Yu Hu
Summary: The study suggests that circSLC8A1 plays a role in breast cancer by regulating the miR-671 and PTEN/PI3k/Akt signaling pathways, leading to inhibition of tumor growth and metastasis.
Article
Pharmacology & Pharmacy
Kai Zhang, Zixiang Liu, Yingchuang Tang, Xiaofeng Shao, Xi Hua, Hao Liu, Huilin Yang, Kangwu Chen
Summary: Chordoma is a rare malignant bone tumor with unclear mechanisms. lncRNA NONHSAT114552 plays a critical role in its development by regulating NRP1 through directly sponging miR-320d. This study highlights NONHSAT114552 as a potential prognostic marker and therapeutic target for Chordoma.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Cell Biology
Jian Zhang, Shen Hu, Rui Ding, Jinghong Yuan, Jingyu Jia, Tianlong Wu, Xigao Cheng
Summary: The study revealed a novel circSNHG5-miR-495-3p axis involved in IDD progression, with circSNHG5 regulating cell proliferation and extracellular matrix degradation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Ibrahim Elsaeid Elnour, Xiaogang Wang, Toremurat Zhansaya, Zhanerke Akhatayeva, Rajwali Khan, Jie Cheng, Yongzhen Hung, Xianyong Lan, Chuzhao Lei, Hong Chen
Summary: In this study, circMYL1 was shown to inhibit proliferation and promote differentiation of bovine myoblast by sponging miR-2400. The interactions between circMYL1 and miR-2400 were demonstrated through luciferase screening and RNA immunoprecipitation assays. Rescue experiments indicated that circMYL1 may counteract the effects of miR-2400 on myoblast proliferation and differentiation.
Article
Agriculture, Dairy & Animal Science
Chengchuang Song, Qi Wang, Qi Qi, Xi Chen, Yanhong Wang, Chunlei Zhang, Fang Xintang
Summary: The study revealed that miR-495-3p inhibits proliferation and promotes differentiation in muscle cells by targeting CDH2. This finding sheds light on the crucial regulatory role of miR-495-3p in skeletal muscle development.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Hongfei Liu, Hongwei Xu, Xianyong Lan, Xiukai Cao, Chuanying Pan
Summary: Three InDel loci within the IGF2BP1 gene were significantly associated with growth traits in sheep, especially in terms of body weight. Heterozygous individuals at these loci exhibited superior growth performance. These crucial variants can be used in marker-assisted selection in sheep.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Xiaogang Wang, Yiru Wang, Xiukai Cao, Yongzhen Huang, Pi Li, Xianyong Lan, Chaogetu Buren, Linyong Hu, Hong Chen
Summary: This study detected the presence and distribution of three CNV regions within the KAT6A gene using quantitative polymerase chain reaction in different sheep breeds. The results showed significant associations between these CNVs and body measurements in certain breeds, suggesting that they could be used as candidate markers for molecular breeding in these breeds.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Genetics & Heredity
Xiaoyang Lv, Weihao Chen, Shanhe Wang, Xiukai Cao, Zehu Yuan, Tesfaye Getachew, Joram M. Mwacharo, Aynalem Haile, Wei Sun
Summary: This study investigates the potential roles of miRNAs in hair follicles with different patterns in Hu sheep lambskin. Key miRNAs and miRNA-mRNA pairs are identified, suggesting their potential effects on wool curvature through direct or indirect regulation of hair follicle development.
Article
Genetics & Heredity
Yue Li, Xiaoyang Lv, Shanhe Wang, Xiukai Cao, Zehu Yuan, Tesfaye Getachew, Joram M. Mwacharo, Aynalem Haile, Wei Sun
Summary: BMP7 plays an important role in hair follicle growth and development in Hu sheep. It regulates the proliferation and cell cycle of dermal papilla cells (DPCs) and may influence hair follicle growth and development via the TGF-beta/Smad signaling pathway.
Article
Cell Biology
Yeqing Ren, Biao Yang, Geng Guo, Jianping Zhang, Yanqi Sun, Dong Liu, Shihao Guo, Yongqiang Wu, Xiaogang Wang, Shule Wang, Wenju Zhang, Xiaolong Guo, Xuepeng Li, Ren Li, Jianhang He, Zihan Zhou
Summary: GBP2 plays a critical role in glioma by regulating the EGFR signaling pathway and interacting with KIF22, which suggests its potential as a therapeutic target for glioma treatment.
CELL DEATH DISCOVERY
(2022)
Review
Clinical Neurology
Ji Jin, Geng Guo, Yeqing Ren, Biao Yang, Yongqiang Wu, Shule Wang, Yanqi Sun, Xiaogang Wang, Yuxiao Wang, Jianzhong Zheng
Summary: A meta-analysis was conducted to identify the risk factors for the recurrence of intracranial aneurysm after coil embolization. The study found that intracranial aneurysms in the middle cerebral artery and posterior circulation, as well as those larger than 7 mm, ruptured, and associated with subarachnoid hemorrhage, had a significantly higher risk of recurrence after coil embolization.
FRONTIERS IN NEUROLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Shan Wang, Yifei Gu, Xiukai Cao, Ling Ge, Mingliang He, Weibo Zhang, Tesfaye Getachew, Joram M. Mwacharo, Aynalem Haile, Kai Quan, Yutao Li, Antonio Reverter, Wei Sun
Summary: This study aimed to identify the target genes of IGFBP3 and investigate their effects on the proliferation and differentiation of Hu sheep skeletal muscle cells. IGFBP3 is an RNA-binding protein that regulates mRNA stability. We predicted the target genes of IGFBP3 through bioinformatics analysis and experimental verification, and demonstrated GNAI2 as one of its target genes. After interference with siRNA, we found that GNAI2 can promote the proliferation and inhibit differentiation of Hu sheep skeletal muscle cells. This study revealed the effects of GNAI2 and provided insights into the regulatory mechanisms of IGFBP3 in sheep muscle development.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Genetics & Heredity
Hui Zhou, Sainan Huang, Xiaoyang Lv, Shanhe Wang, Xiukai Cao, Zehu Yuan, Tesfaye Getachew, Joram M. M. Mwacharo, Aynalem Haile, Kai Quan, Yutao Li, Antonio Reverter, Wei Sun
Summary: The study found that CUX1 plays an important role in hair follicle growth and development. Overexpression and knockdown of CUX1 gene showed that CUX1 promotes dermal papilla cell proliferation and affects the expression of key genes in the Wnt/beta-catenin signaling pathway.
Article
Agriculture, Dairy & Animal Science
Jie Cheng, Xiukai Cao, Xiaogang Wang, Jian Wang, Binglin Yue, Wei Sun, Yongzhen Huang, Xianyong Lan, Gang Ren, Chuzhao Lei, Hong Chen
Summary: This study provides the first 3D genome data in Longissimus dorsi muscle of fetal and adult cattle in China. The research reveals the dynamics of genome structure and its correlation with transcriptomic divergence during muscle development. It also annotates the regulatory elements in cattle genome during myogenesis, demonstrating the enrichment of promoter and enhancer in selection sweeps.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Weihao Chen, Xiaoyang Lv, Xiukai Cao, Zehu Yuan, Shanhe Wang, Tesfaye Getachew, Joram M. Mwacharo, Aynalem Haile, Kai Quan, Yutao Li, Wei Sun
Summary: This study revealed the interplay between intestinal genes, metabolites, and bacteria in E. coli F17-infected sheep. The results showed significant differences in the intestines of sheep with different E. coli F17 susceptibility, and identified potential biomarkers for E. coli F17 infection. These findings can contribute to the development of new treatments for farm animals infected with E. coli F17.
Article
Agriculture, Dairy & Animal Science
Mingliang He, Xiaoyang Lv, Xiukai Cao, Zehu Yuan, Kai Quan, Tesfaye Getachew, Joram M. M. Mwacharo, Aynalem Haile, Yutao Li, Shanhe Wang, Wei Sun
Summary: In our study, we found that CRABP2 was highly expressed in Hu sheep hair follicles, indicating its potential influence on dermal papilla cells (DPCs). Our experiments demonstrated that overexpression of CRABP2 promoted DPC proliferation and activated the Wnt/beta-catenin pathway. These findings highlight the crucial role of CRABP2 in Hu sheep DPC proliferation and shed light on its function in hair follicles.
Article
Veterinary Sciences
Shanhe Wang, Tingyan Hu, Mingliang He, Yifei Gu, Xiukai Cao, Zehu Yuan, Xiaoyang Lv, Tesfaye Getachew, Kai Quan, Wei Sun
Summary: In this study, the properties of ovine DPCs were investigated, showing that they have strong aggregation and stable alkaline phosphatase activity even after multiple passages in culture. Differences in marker gene expression and Wnt/β-catenin signaling activity were observed between early passaged DPCs and multiple passaged DPCs. It was demonstrated that Wnt/β-catenin signaling regulates cell aggregation and alkaline phosphatase activity through the FGF and IGF signaling pathways.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Genetics & Heredity
Xiaoyang Lv, Yue Li, Weihao Chen, Shanhe Wang, Xiukai Cao, Zehu Yuan, Tesfaye Getachew, Joram Mwacharo, Aynalem Haile, Yutao Li, Wei Sun
Summary: This study explored the potential association between DNA methylation of CUX1 core promoter region and lambskin pattern in Hu sheep. The results showed no significant difference in methylation levels between different pattern tissues. However, the identification of the core region and a potential repressive region of CUX1 promoter enriched the understanding of CUX1's role in hair follicle development in Hu sheep.
Article
Agriculture, Dairy & Animal Science
Zehu Yuan, Ling Ge, Pengwei Su, Yifei Gu, Weihao Chen, Xiukai Cao, Shanhe Wang, Xiaoyang Lv, Tesfaye Getachew, Joram M. Mwacharo, Aynalem Haile, Wei Sun
Summary: This study reveals the direct role of NCAPG in regulating myogenesis in sheep embryonic myoblast cells and identifies the association between SNPs in its promoter region and sheep growth traits.
Article
Multidisciplinary Sciences
XiuKai Cao, Jie Cheng, Shan Wang, Wei Sun
Summary: Improved primers can enhance the recombinant efficiency of psi-CHECK2 compared to traditional primers, as demonstrated by increased bacterial colony numbers, PCR amplification positive rates, and confirmed recombinant efficiency through Sanger sequencing. Additionally, endogenous homologous recombination can promote the recombinant efficiency of psi-CHECK2, although to a lesser extent than the combination of improved primers and T4 ligase treatment.