期刊
MOLECULAR THERAPY-NUCLEIC ACIDS
卷 16, 期 -, 页码 605-615出版社
CELL PRESS
DOI: 10.1016/j.omtn.2019.04.009
关键词
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资金
- National Natural Science Foundation of China [81771603, 81801472]
- Postgraduate Research & Practice Innovation Program of Jiangsu Province [KYCX18_1481]
- Natural Science Foundation of Jiangsu Province [BK20171502, BK20161061, BK20181080]
- National Key Research Plan of China [2018YFC1002205]
Long noncoding RNAs (lncRNAs) have been reported to be involved in various human diseases, and increasing studies have revealed that lncRNAs can play a vital role in preeclampsia (PE). In our study, 1ncRNA hypoxia-inducible factor 1 alpha (HIF1A) antisense RNA 2 (HIF1A-AS2) was found to be significantly downregulated in placenta tissues of PE patients by quantitative real-time PCR analysis. Moreover, Cell Counting Kit-8 (CCK-8) assays showed that downregulation of HIF1A-AS2 can impede cell proliferation of HTR-8/SVneo and JAR trophoblasts cells. Ectopic overexpression of HIF1A-AS2 can increase the function of trophoblasts cell migration and invasion in vitro. RNA-sequencing (RNA-seq), RNA immunoprecipitation (RIP), and chromatin immunoprecipitation (ChIP) experiments showed that HIF1A-AS2 can recruit lysine-specific demethylase 1 (LSD1) and epigenetically repress pleckstrin homology-like domain, family A, member 1 (PHLDA1) transcription in human trophoblasts cells. In summary, our findings suggest that downregulated HIF1A-AS2 may play a role in the pathogenesis and progression of PE, and has potential as a novel prognostic marker in PE.
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