Article
Microbiology
Men Thi Ngo, Minh Van Nguyen, Jae Woo Han, Myung Soo Park, Hun Kim, Gyung Ja Choi
Summary: The culture filtrate of Trichoderma longibrachiatum SFC100166 and its metabolites showed potential antifungal effects against Phytophthora infestans, the pathogen causing late blight. New compound spirosorbicillinol D and natural compound 2',3'-dihydro-epoxysorbicillinol exhibited good inhibition effects. Bisvertinolone had a significant impact in reducing the development of tomato late blight in in vitro antifungal assays.
Article
Biochemistry & Molecular Biology
Shu-Hua Zhang, Xian-Lin Yue, Xue Zhao, Jing Tang, Yu Yang, Rui Xu, Hao Ma, Shuai-Ming Zhu, Fu-Yao Luo, Qin Zhang, Guo-Gang Zhang, Chang-Wei Li
Summary: Longibrachiamide A is a newly discovered peptide with strong cytotoxicity and moderate antibacterial activity against various cells and bacteria.
CHEMISTRY & BIODIVERSITY
(2022)
Article
Biochemistry & Molecular Biology
Shu-Hua Zhang, Jiewei Yang, Hao Ma, Yu Yang, Guo-Feng Zhou, Xue Zhao, Rui Xu, Dan Nie, Guo-Gang Zhang, Jun-Jie Shan, Cheng-Bin Cui, Chang-Wei Li
Summary: Five new peptaibols, longibramides A-E, were isolated from a fungus collected from the subboreal area of northeast China. The structures and absolute configurations of these peptaibols were determined through spectroscopic data and X-ray diffractions. Longibramides B and E showed moderate cytotoxicities and inhibitory effects against MRSA T144, with unique amino acid residues not commonly found in natural peptaibols.
CHEMISTRY & BIODIVERSITY
(2021)
Article
Microbiology
Rocco Caracciolo, Luca Sella, Marta De Zotti, Angela Bolzonello, Marco Armellin, Livio Trainotti, Francesco Favaron, Silvio Tundo
Summary: Black rot, caused by Xanthomonas campestris pv. campestris (Xcc), is a destructive disease affecting crucifers. Copper-based products, despite their potential negative effects, are currently used to manage the pathogen. Trichoderma spp. has been used as a biocontrol agent against various phytopathogens. In this study, synthetic analogs of the peptaibol trichogin GA IV were tested for their antimicrobial activity against Xcc and other bacteria, and one of the peptides (4r) showed effectiveness in reducing black rot symptoms on cauliflower plants. The cytotoxic activity of peptide 4r on tobacco cells was also evaluated.
Article
Biochemistry & Molecular Biology
Zhiyan Shen, Shuwu Zhang, Xu Bingliang
Summary: In this study, the endochitinase 18-5 gene (T6-Echi18-5) from Trichoderma longibrachiatum T6 was cloned and its role in degrading the eggshell of Heterodera avenae and inhibiting eggs hatching was investigated.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemical Research Methods
John B. Coulton, James L. Edwards
Summary: The sample preparation technique described in the study involves online filter-assisted electroporation-based cell lysis and chilled organic solvent extraction for rapid preparation of metabolomic samples from suspended cells. By using isotopically labeled internal standards and C-13-glucose labeling experiments, the method ensures enzymatic quenching and successful incorporation of C-13 into organic acid metabolites. Analysis by nano-reverse-phase liquid chromatography-mass spectrometry (nano-RP-LC-MS) demonstrates the effectiveness of the technique in studying metabolites such as itaconate.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Medicine, Research & Experimental
Taylor A. Dodson, Stephan Nieuwoudt, Chase N. Morse, Valinteshley Pierre, Chao Liu, Samuel E. Senyo, Erin G. Prestwich
Summary: We evaluated tRNA modifications in the diabetic heart and found that tRNA modifications play an important role in diabetic etiologies. We quantified tRNA modifications and ribonucleoside levels in healthy and diabetic murine hearts and hyperglycemic cell models. Results showed abnormal ribonucleoside modifications in hyperglycemic models and diabetic tissues. Our findings suggest that tRNA modifications may regulate translation in diabetes.
Article
Biotechnology & Applied Microbiology
Zhen Li, Kleinberg X. Fernandez, John C. Vederas, Michael G. Ganzle
Summary: This study aims to investigate the interaction between Bacillus species and fungi in daqu models. It was found that B. velezensis FUA2155 displayed strong antifungal activity in the daqu model and was the only strain that produced multiple iturin A congeners in situ.
Article
Biochemistry & Molecular Biology
Dora Balazs, Tamas Marik, Andras Szekeres, Csaba Vagvolgyi, Laszlo Kredics, Chetna Tyagi
Summary: This study investigated the production of peptaibols by various Trichoderma strains and their correlation with structure-activity relationships. The results showed that T. longibrachiatum f. bissettii SZMC 12546 exerted the lowest minimum inhibitory concentrations (MIC) against Gram-positive bacteria and also inhibited the plant pathogenic Gram-negative Rhizobium radiobacter. Molecular dynamics simulations revealed that the folding dynamics of Group A peptaibols were more restricted and had well-folded helical conformations, which may contribute to their bioactivity against bacteria.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Jan Viglas, Simona Dobiasova, Jitka Viktorova, Tomas Ruml, Vanda Repiska, Petra Olejnikova, Helena Gbelcova
Summary: This study investigated the production of peptaibols by Trichoderma atroviride O1 and their antibacterial and anticancer activity against drug-sensitive and multi-drug resistant bacteria and cancer cell lines. The results showed that peptaibols exhibited significant inhibitory effects on the growth of methicillin-resistant Staphylococcus aureus and human breast cancer and ovarian cancer cell lines.
Article
Chemistry, Analytical
Kendall R. Johnson, Yunfan Gao, Michal Gregus, Alexander R. Ivanov
Summary: This study presents a specialized method for proteomic analysis of limited samples and single cells, which involves processing intact cells directly in the separation capillary. The results demonstrate the effectiveness of this method in analyzing low numbers of mammalian cancer cells and single cells, with high identification numbers and proteoform quantities.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Kendall R. Johnson, Yunfan Gao, Michal Gregus, Alexander R. Ivanov
Summary: Proteomic analysis of limited samples and single cells requires specialized methods. In this study, a method combining capillary electrophoresis and tandem mass spectrometry was used to directly process intact cells without sample handling and transfer. The results demonstrated that this method has high sensitivity and sample retention for analyzing low numbers of cells.
ANALYTICAL CHEMISTRY
(2022)
Article
Plant Sciences
Abraham Hernandez-Ceja, Pedro Damian Loeza-Lara, Francisco Javier Espinosa-Garcia, Yolanda M. Garcia-Rodriguez, Jose Roberto Medina-Medrano, German Fernando Gutierrez-Hernandez, Luis Fernando Ceja-Torres
Summary: Three pathogenic fungi of blueberry were isolated in Michoacan, Mexico, and extracts from medicinal plants such as Lantana hirta, Argemone ochroleuca, and Adenophyllum porophyllum were found to inhibit their growth in vitro. These extracts contain potential antifungal compounds that could be further explored for their efficacy against plant pathogens.
Article
Multidisciplinary Sciences
Solomon Boamah, Shuwu Zhang, Bingliang Xu, Tong Li, Alejandro Calderon-Urrea, Richard John Tiika
Summary: This study investigated the potential of the plant-growth-promoting fungus Trichoderma longibrachiatum (TG1) to enhance plant growth and physiological responses in wheat seedlings under salt stress. The results showed that TG1 treatment significantly increased germination rate, seedling growth, salicylic acid (SA) content, and antioxidant enzyme activity in wheat seedlings, as well as upregulated the expression of stress-responsive genes under salt stress conditions.
Article
Plant Sciences
Jin Woo Lee, Jennifer E. Collins, Karen L. Wendt, Debopam Chakrabarti, Robert H. Cichewicz
Summary: Malaria, affecting over 200 million people annually, remains a global threat. Through systematic testing of fungal extracts from the University of Oklahoma Citizen Science Soil Collection Program, bioactive compounds with potent antiplasmodial activity and low toxicity against human cells were identified, with a new metabolite, harzianin NPDG I, showing remarkable potency and selectivity. This study highlights the potential of fungal diversity in developing novel anti-malarial compounds.
JOURNAL OF NATURAL PRODUCTS
(2021)
Article
Biochemistry & Molecular Biology
Attila Bartal, Huynh Thu, Anita Kecskemeti, Monika Voros, Orsolya Kedves, Henrietta Allaga, Monika Varga, Laszlo Kredics, Csaba Vagvoelgyi, Andras Szekeres
Summary: Surfactins are cyclic lipopeptides consisting of a beta-hydroxy fatty acid and a peptide ring. They have antibacterial, antifungal, and antiviral activities and can be produced by Bacillus species. Bacillus strains isolated from vegetable rhizospheres were identified and tested for surfactin production and inhibition on phytopathogen bacteria and fungi. An HPLC-HESI-MS/MS method was used for quantitative and qualitative analysis, revealing the production of 29 surfactin variants and homologues by more than half of the tested Bacillus strains.
Article
Food Science & Technology
Gabor Endre, Babett Edit Nagy, Daniel Hercegfalvi, Csenge Kasuba, Csaba Vagvolgyi, Andras Szekeres
Summary: Aflatoxins (AFs) are secondary metabolites that cause diseases in animals and humans. Regulations set concentration limits for AFs in EU food and feed products, requiring pure forms for reference standards. Improvements were made to a liquid-liquid chromatographic method to enhance purification and increase the yield of pure AFs. Through a scale-up process, approximately 2.2 g of AFs could be purified using 8.2 L of solvent in a 250 mL rotor, while 7.8 g of AFs could be prepared using 31 L of solvent in a 1000 mL rotor.
Article
Food Science & Technology
David Rakk, Jozsef Kukolya, Biljana D. Skrbic, Csaba Vagvolgyi, Monika Varga, Andras Szekeres
Summary: In this study, a 1-minute multi-mycotoxin analysis method was developed using high-resolution equipment and a short column. This method was able to detect 11 mycotoxins in corn and wheat matrices within one minute, utilizing the multiplexing and high-resolution capability of the QExactive Orbitrap MS.
Article
Microbiology
Liqiong Chen, Simang Champramary, Neha Sahu, Boris Indic, Attila Szucs, Gabor Nagy, Gergely Maroti, Bernadett Pap, Omar Languar, Csaba Vagvolgyi, Laszlo G. Nagy, Laszlo Kredics, Gyorgy Sipos
Summary: This study analyzed the antagonistic and defense mechanisms of a soilborne fungus against destructive forest pathogens. The results showed that the antagonistic fungus inhibited the growth of the pathogenic fungus through multiple biocontrol mechanisms, while the pathogenic fungus protected itself from fungal invasion through multiple defense mechanisms. This study provides insights for further exploration of the interaction mechanisms between plant pathogenic fungi and biocontrol agents.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Viktor David Nagy, Anuar Zhumakayev, Monika Voros, Adam Borde, Adrienn Szarvas, Attila Szucs, Sandor Kocsube, Peter Jakab, Tamas Monostori, Biljana D. Skrbic, Edina Mohai, Lorant Hatvani, Csaba Vagvolgyi, Laszlo Kredics
Summary: The cultivation and consumption of sweet potato are increasing globally, but the use of chemical fertilizers and pesticides may cause pollution. Therefore, there is a need for environmentally-friendly biological solutions. This study developed a microbial inoculant from multiple microorganisms and tested its application in sweet potato cultivation, resulting in increased yield. This is the first successful application of a fungal-bacterial consortium in sweet potato cultivation.
Article
Microbiology
Maria (Aino) Andersson, Camilla Vornanen-Winqvist, Tuomas Koivisto, Andras Varga, Raimo Mikkola, Laszlo Kredics, Heidi Salonen
Summary: During the COVID-19 pandemic, research found that dust samples collected in sport facilities contained a high proportion of pathogenic fungi and a low proportion of bacteria. These potentially pathogenic fungi in the dust samples may pose potential health risks. This is likely the result of the influence of cleaning and disinfection on microbial communities.
Review
Biology
Zoltan Marton Kohler, Agnes Szepesi
Summary: L-aminoguanidine (AG) is widely used as an inhibitor in studying plant abiotic stress responses, but its exact mode of action is not well understood. It has been used as a specific diamine oxidase inhibitor and for reducing nitric oxide production. Recent studies suggest its antiglycation effect, but this remains unclear in plants. This review provides a summary of current knowledge about the different targets of AG in plants and recommends using it as a modulator of polyamine-related mechanisms.
Article
Remote Sensing
Laszlo Bakacsy, Zalan Tobak, Boudewijn van Leeuwen, Peter Szilassi, Csaba Biro, Jozsef Szatmari
Summary: Invasive alien species pose a serious threat to biodiversity and ecosystem services and knowing their distribution and spread dynamics is crucial for effective management. Unmanned aerial vehicle (UAV) monitoring, using RGB color-based vegetation indices, was examined for identifying invasive plant species. The TGI and SSI indices were found to be the most suitable for identifying the cover area of common milkweed, while the IF index was the most suitable for blanket flower. However, the methods used were not suitable for determining milkweed shoot and blanket flower inflorescence number due to significant overestimation.
Article
Biochemistry & Molecular Biology
Agnes Szepesi, Laszlo Bakacsy, Attila Feher, Henrietta Kovacs, Peter Palfi, Peter Poor, Reka Szollosi, Orsolya Kinga Gondor, Tibor Janda, Gabriella Szalai, Christian Lindermayr, Laszlo Szabados, Laura Zsigmond
Summary: Polyamine catabolism mediated by amine oxidases is important for regulating polyamine homeostasis and related mechanisms during salt stress. The effects of L-aminoguanidine (AG), an inhibitor of copper amine oxidases, on tomato roots exposed to short-term salt stress were studied. AG treatment alleviated the increase in reactive oxygen and nitrogen species caused by salt stress, suggesting its possible antioxidant effect.
Article
Biochemistry & Molecular Biology
Dora Balazs, Tamas Marik, Andras Szekeres, Csaba Vagvolgyi, Laszlo Kredics, Chetna Tyagi
Summary: This study investigated the production of peptaibols by various Trichoderma strains and their correlation with structure-activity relationships. The results showed that T. longibrachiatum f. bissettii SZMC 12546 exerted the lowest minimum inhibitory concentrations (MIC) against Gram-positive bacteria and also inhibited the plant pathogenic Gram-negative Rhizobium radiobacter. Molecular dynamics simulations revealed that the folding dynamics of Group A peptaibols were more restricted and had well-folded helical conformations, which may contribute to their bioactivity against bacteria.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Plant Sciences
Krisztina Napsugar Nagy, Luca Viktoria Kardos, Zsofia Orban, Laszlo Bakacsy
Summary: The global spread of invasive plant species poses a significant threat to biodiversity. The Indian blanket flower, widely used as an ornamental plant, exhibits allelopathic effects and has the potential to become invasive.
PLANT SPECIES BIOLOGY
(2023)