期刊
CELL REPORTS
卷 27, 期 13, 页码 3939-+出版社
CELL PRESS
DOI: 10.1016/j.celrep.2019.05.092
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资金
- NIH S10 [S10RR027431-01]
- NIH [R01 NS089533, AG020961]
- California Institute for Regenerative Medicine grant [RB5-07469]
- Baxter Foundation
- National Science Foundation Graduate Research Fellowship Program (GRFP) [1309047]
- Canadian Institutes of Health Research
- NIH Postdoctoral Individual National Research Service Award [F32 GM112425-02]
- BD Biosciences Stem Cell grant
The impact of glucose metabolism on muscle regeneration remains unresolved. We identify glucose metabolism as a crucial driver of histone acetylation and myogenic cell fate. We use single-cell mass cytometry (CyTOF) and flow cytometry to characterize the histone acetylation and metabolic states of quiescent, activated, and differentiating muscle stem cells (MuSCs). We find glucose is dispensable for mitochondria! respiration in proliferating MuSCs, so that glucose becomes available for maintaining high histone acetylation via acetyl-CoA. Conversely, quiescent and differentiating MuSCs increase glucose utilization for respiration and have consequently reduced acetylation. Pyruvate dehydrogenase (PDH) activity serves as a rheostat for histone acetylation and must be controlled for muscle regeneration. Increased PDH activity in proliferation increases histone acetylation and chromatin accessibility at genes that must be silenced for differentiation to proceed, and thus promotes self-renewal. These results highlight metabolism as a determinant of MuSC histone acetylation, fate, and function during muscle regeneration.
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