Article
Nanoscience & Nanotechnology
Kai Chen, Tao Yu, Xin Wang
Summary: Research found that exosome-derived miRNA from diabetic patients slows down wound healing process by suppressing vascular endothelial growth factor A expression.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Review
Biochemistry & Molecular Biology
George Bibby, Blerta Krasniqi, Izaak Reddy, Durairaj Sekar, Kehinde Ross
Summary: Inhibition strategies targeting miRNAs can promote wound healing, and comparing chemical modifications of different miRNAs is essential to determine the most translationally viable options.
Review
Immunology
Samuel Knoedler, Sonja Broichhausen, Ruiji Guo, Ruoxuan Dai, Leonard Knoedler, Martin Kauke-Navarro, Fortunay Diatta, Bohdan Pomahac, Hans-Guenther Machens, Dongsheng Jiang, Yuval Rinkevich
Summary: Injuries to the skin initiate a series of synchronized healing processes. Fibroblasts play various roles in endogenous wound repair, including activating innate immune cells, depositing scar tissue, and transporting fascial connective tissue to the wounds. Understanding the diversity and versatility of fibroblasts is crucial for deciphering wound pathologies and developing new treatment approaches.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Medicine, Research & Experimental
Shashi Bala, Yuan Zhuang, Prashanth Thevkar Nagesh, Donna Catalano, Adam Zivny, Yanbo Wang, Jun Xie, Guangping Gao, Gyongyi Szabo
Summary: This study investigated the therapeutic effect of miR-155 inhibition in liver fibrosis and found that miR-155 deficiency can significantly reduce liver fibrosis. The study also revealed that miR-155 regulates activation of hepatic stellate cells partly via STAT3 signaling.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Article
Immunology
Christopher O. Audu, William J. Melvin, Amrita D. Joshi, Sonya J. Wolf, Jadie Y. Moon, Frank M. Davis, Emily C. Barrett, Kevin D. Mangum, Hongping Deng, Xianying Xing, Rachel Wasikowski, Lam C. Tsoi, Sriganesh B. Sharma, Tyler M. Bauer, James Shadiow, Matthew A. Corriere, Andrea T. Obi, Steven L. Kunkel, Benjamin Levi, Bethany B. Moore, Johann E. Gudjonsson, Andrew M. Smith, Katherine A. Gallagher
Summary: JMJD3 plays a central role in the regulation of macrophage plasticity during tissue repair, and its dysregulation in diabetic wounds leads to persistent inflammation. IL-6, through the JAK1,3/STAT3 pathway, regulates JMJD3 expression in diabetic wound macrophages and contributes to inflammatory gene transcription. JMJD3 also regulates the STING gene, which plays a dual role in macrophage inflammation during wound healing and in diabetic wounds.
CELLULAR & MOLECULAR IMMUNOLOGY
(2022)
Article
Medicine, Research & Experimental
Lishuang Li, Yuman Ma, Gaiying He, Shuhua Ma, Yi Wang, Yanan Sun
Summary: Granulation tissue supports and fills wounds during healing. Collagen produced by fibroblasts acts as a scaffold, facilitating the formation of new blood vessels and epithelial coverage. Protein components extracted from pilose antler were found to contain extracellular matrix components and were used in an experiment to determine their effects on collagen content and fiber synthesis during wound healing. The experiment showed that the extract promoted collagen synthesis, reduced degradation, and upregulated TGF-β expression, thus accelerating wound healing.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Review
Biochemistry & Molecular Biology
Thayaalini Subramaniam, Mh Busra Fauzi, Yogeswaran Lokanathan, Jia Xian Law
Summary: Skin injury healing involves complex processes with various cells, extracellular matrix, and mediators. Cell differentiation, migration, and proliferation are crucial for tissue integrity restoration. Although calcium shows promise in wound healing, challenges need to be addressed before clinical application.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Endocrinology & Metabolism
Xiaolei Wang, Yanbin Gao, Wenming Yi, Yu Qiao, Hao Hu, Ying Wang, Yan Hu, Shuxin Wu, Hongfeng Sun, Taojing Zhang
Summary: miRNA-155 expression is elevated in diabetic nephropathy and cultured podocytes, inhibiting miRNA-155 can reduce proteinuria and ACR levels, improve kidney function, and suppress podocyte inflammation and injury through SIRT1 silencing.
JOURNAL OF DIABETES RESEARCH
(2021)
Review
Immunology
Yuanyuan Jiang, Xiang Xu, Long Xiao, Lihong Wang, Sheng Qiang
Summary: This article discusses the role and significance of microRNA in the inflammatory process of wound healing. It points out that the initiation and resolution of the inflammatory response are crucial for wound healing and may provide a new direction for wound treatment.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Nuo-qi Wang, Wei-hua Jia, Lin Yin, Na Li, Mei-dai Liang, Jia-min Shang, Bi-yu Hou, Li Zhang, Gui-fen Qiang, Guan-hua Du, Xiu-ying Yang
Summary: This study investigates the influence of diabetes and sex on the expression patterns of FGFs in normal and diabetic wound healing in mice. The expression patterns of Fgfs were affected by sex in both normal and diabetic mice during wound healing. In addition, sex differentially influenced the expression patterns of Fgfrs in non-wound skin and wounds in both normal and diabetic mice. Female mice had larger wound areas in the early stage of wound healing.
MOLECULAR BIOLOGY REPORTS
(2023)
Review
Cell Biology
Kehinde Ross
Summary: miRNAs play a critical role in wound healing, particularly in influencing the behavior of epidermal keratinocytes. Combining multiple miRNA mimics targeting different but complementary wound healing processes may be more effective in promoting wound repair.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Review
Endocrinology & Metabolism
Hongjie Li, Shengyu Jing, Hongbo Xu
Summary: The difficulty of wound healing in diabetes mellitus is a long-standing problem in the medical field, mainly due to the abnormal function of wound-related cells. Recent studies have shown that microRNA (miR), a noncoding RNA present in eukaryotic cells, plays a crucial role in the functions of various cells involved in diabetic wound healing. This paper establishes a cellular perspective on the connection between miR and wound healing, summarizing the effects of different miRs on wound cells, including their targets and related mechanisms. Abnormal expression of miRs in wounds holds potential for early diagnosis of diabetic wounds, while correcting or enhancing specific miRs may have therapeutic value in promoting wound healing.
JOURNAL OF DIABETES
(2023)
Article
Immunology
Shiyan Li, Xiaofeng Ding, Hao Zhang, Youjun Ding, Qian Tan
Summary: IL-25 plays a role in the treatment of diabetic wounds by promoting macrophage and fibroblast activation, affecting inflammation resolution through multiple signaling pathways, and improving angiogenesis and collagen alignment.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Medicine, Research & Experimental
Piyush Gondaliya, Adil Ali Sayyed, Palak Bhat, Mukund Mali, Neha Arya, Amit Khairnar, Kiran Kalia
Summary: Diabetic wounds are a debilitating complication affecting a significant number of patients, and current therapies are unsatisfactory. This study demonstrates the potential of miR-155 inhibitor-loaded MSC-derived exosomes in promoting diabetic wound healing through synergistic effects. The study also suggests a new approach of using MSC-derived exosomes to encapsulate miRNA and antibiotics for improved management of diabetic wounds.
MOLECULAR PHARMACEUTICS
(2022)
Article
Pharmacology & Pharmacy
Xiaochen Li, Cheng Meng, Fei Han, Juhong Yang, Jingyu Wang, Yanjuan Zhu, Xiao Cui, Minxia Zuo, Jie Xu, Baocheng Chang
Summary: Vildagliptin prevents diabetic cardiomyopathy by restoring autophagy through the miR-21/SPRY1/ERK/mTOR pathway. Overexpression of miR-21 attenuates the protective effect of vild on cardiac function.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Medical Laboratory Technology
Paivi Lakkisto, Louise Torp Dalgaard, Thalia Belmonte, Sara-Joan Pinto-Sietsma, Yvan Devaux, David De Gonzalo-Calvo
Summary: The implementation of cell-free microRNAs as biomarkers is limited due to lack of reproducibility. Technical variability and analytical caveats make the interpretation of miRNA data challenging. Standardization efforts and consideration of specific aspects in sample handling and data analysis are important for reproducibility. Providing a checklist can facilitate the control of miRNA quantification and analysis.
CRITICAL REVIEWS IN CLINICAL LABORATORY SCIENCES
(2023)
Review
Cell Biology
Amila Iriskic Atic, Maja Thiele, Alexander Munk, Louise Torp Dalgaard
Summary: MicroRNAs (miRNAs) secreted by the liver have the potential to be important biomarkers for nonalcoholic liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH). Several miRNAs, including miR-122, miR-21, miR-34a, miR-192, miR-193, and the miR-17-92 miRNA-cluster, are consistently associated with NAFLD/NASH. These miRNAs show potential as diagnostic markers for NAFLD/NASH, although more research is needed to understand their specificity and regulation.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Endocrinology & Metabolism
Ermelindo C. Leal, Tatiana Emanuelli, Diana Santos, Joao Moura, Ana Catarina Rg Fonseca, Ana Burgeiro, Eugenia Carvalho
Summary: This study investigated the impact of alterations in the unfolded protein response (UPR), autophagy, and mitochondrial function on wound healing (WH) under diabetic conditions. The findings showed that the UPR and autophagy pathways were activated in the skin of control mice and non-wounded skin of diabetic mice. Additionally, high-glucose conditions induced autophagy in keratinocyte and fibroblast cell cultures. However, there were no changes in mitophagy in the skin of diabetic mice or wounded skin.
JOURNAL OF MOLECULAR ENDOCRINOLOGY
(2023)
Article
Medicine, Research & Experimental
Diana Santos, Patricia Porter-Gill, Grace Goode, Leanna Delhey, Anja Elaine Sorensen, Shannon Rose, Elisabet Borsheim, Louise Torp Dalgaard, Eugenia Carvalho
Summary: The aim of this study was to identify a circulating miRNA profile that reflects insulin resistance in prepubertal children with obesity. Plasma miRNAs of the participants were measured and subjects were divided into normal weight and overweight or obese groups. The study found differential expression of eleven miRNAs between the two groups, and miR-146a-5p was correlated with insulin resistance. These findings may contribute to early interventions and treatments.
Article
Biochemistry & Molecular Biology
Vivek Kumar, Jennifer S. Lin, Natalia Molchanova, John A. Fortkort, Carolin Reckmann, Stefan Braese, Havard Jenssen, Annelise E. Barron, Archana Chugh
Summary: Visceral leishmaniasis (VL) is a neglected tropical disease and the use of cell-permeating peptides as potential drug candidates to treat VL is limited due to their limited biological stability. In this study, peptoids, which are peptide mimics with good biostability, were tested against Leishmania donovani. The addition of N-alkyl hydrophobic chains and bromination yielded compounds with good antileishmanial activity against both intracellular amastigotes and promastigotes, indicating the potential of these peptoids as drug candidates for VL.
Article
Biochemistry & Molecular Biology
Miguel Cifuentes Acebal, Louise Torp Dalgaard, Tue Sparholt Jorgensen, Benni Winding Hansen
Summary: This study investigated the transcriptional changes during embryonic development in Acartia tonsa, a copepod species. The researchers found sequences of developmental genes and described the molecular changes and gene expression levels throughout the 42-hour embryonic development. The findings contribute to the use of A. tonsa as a model organism for crustacean development.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2023)
Review
Biochemistry & Molecular Biology
Sofie Dinesen, Alisar El-Faitarouni, Nanna Lond Skov Frisk, Anja Elaine Sorensen, Louise Torp Dalgaard
Summary: Gestational diabetes mellitus (GDM) is a severe complication during pregnancy, and identifying potential biomarkers for its early detection is crucial. This study conducted a systematic review and meta-analysis to investigate miRNAs associated with GDM. The findings suggest that certain miRNAs show altered expression in GDM, indicating their potential as biomarkers for early GDM detection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Jessica Da Silva, Ermelindo C. Leal, Eugenia Carvalho, Eduardo A. Silva
Summary: The imbalance of local and systemic factors in individuals with diabetes mellitus delays wound healing and leads to diabetic foot ulceration. Biomaterial-based wound dressings with unique properties may be used as a therapeutic strategy for chronic wound healing and DFU management.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Pernille B. Udesen, Anja E. Sorensen, Rikke Svendsen, Nanna L. S. Frisk, Anne L. Hess, Mubeena Aziz, Marie Louise M. Wissing, Anne Lis M. Englund, Louise T. Dalgaard
Summary: In a 6-year follow-up study, it was found that the levels of certain miRNAs in women with PCOS changed, indicating a shift towards a less hyperandrogenic profile. These findings contribute to our understanding of PCOS and provide potential biomarkers for future research.
Review
Biochemistry & Molecular Biology
Nanna Lond Skov Frisk, Anja Elaine Sorensen, Ole Birger Vesterager Pedersen, Louise Torp Dalgaard
Summary: In this study, a systematic review and meta-analysis were conducted to evaluate the global research potential of different circulating miRNAs as an early diagnostic biomarker for ovarian cancer (OC). The primary search resulted in 1887 articles, and 44 relevant studies were identified. Nine miRNAs were found to be dysregulated in OC patients compared to controls, with miR-21, -125, -141, -145, -205, -328, -200a, -200b, and -200c being upregulated. Future studies of circulating miRNAs in relation to OC should consider factors such as sample size, consensus guidelines, and coverage of previously reported miRNAs.
Review
Infectious Diseases
Ines D. Teixeira, Eugenia Carvalho, Ermelindo C. Leal
Summary: Diabetic foot ulcers (DFU) are a serious complication of diabetes, affecting approximately 15% of patients and leading to lower limb amputations. However, impaired healing process and antimicrobial resistance pose challenges to effective treatment. This review explores the use of antimicrobial peptides derived from environmentally friendly processes as potential therapeutic compounds for DFUs, offering better quality of life to patients while preserving bio-resources.
Review
Food Science & Technology
Alphonse Laya, Honore Wangso, Iva Fernandes, Raphael Djakba, Joana Oliveira, Eugenia Carvalho
Summary: This study explores the potential use of traditional fermented condiments as an adjuvant treatment for obesity and type 2 diabetes. The collected data on bioactive compounds in these condiments demonstrate their promising antiobesity and antidiabetic effects, both in animal, human, and clinical studies.
JOURNAL OF FOOD QUALITY
(2023)
Article
Chemistry, Multidisciplinary
Hari Chandana Yadavalli, Sooyeon Park, Yeolhoe Kim, Riddhi Nagda, Tae-Hwan Kim, Min Kyun Han, Il Lae Jung, Yong Joo Bhang, Won Ho Yang, Louise Torp Dalgaard, Seong Wook Yang, Pratik Shah
Summary: A novel method for sensing target microRNAs using innovative Tailed-Hoogsteen triplex DNA-encapsulated Silver Nanoclusters (DNA/AgNCs) is presented. Upon hybridization of specific microRNA with the tail, the Tailed-Hoogsteen triplex DNA/AgNCs exhibit a pronounced red fluorescence, effectively turning on the signal. The underlying mechanism is elucidated and demonstrates the utility of sensors for microRNA imaging with high sensitivity and specificity.
Article
Developmental Biology
Miguel Cifuentes Acebal, Benni Winding Hansen, Tue Sparholt Jorgensen, Louise Torp Dalgaard
Summary: This study compared the transcriptome of quiescent embryos and normal embryonic development in A. tonsa, identifying pathways and differentially regulated transcripts involved in quiescent embryogenesis. The results showed that the expression profile of quiescent embryos was not significantly different from the normal embryonic gastrulation stage, and a large proportion of annotated transcripts displayed expression values between gastrulation and organogenesis stages. Furthermore, comparison with organogenesis stage samples confirmed that a developmental arrest had been induced through quiescence, and stress response transcripts, such as the autophagy gene Sequestosome-1/p62 (SQSTM), were upregulated in quiescence. These findings provide a better understanding of the molecular mechanisms underlying the quiescent embryonic state of A. tonsa.
DEVELOPMENTAL BIOLOGY
(2023)