Quantitative discrimination of biological tissues by micro-elastographic measurement using an epi-illumination Mueller matrix microscope

We propose a method for estimating the stiffness of bio-specimens by measuring linear retardance properties under applied stress. For this purpose, we employ an epi-illumination Mueller matrix microscope and show the procedures for its calibration. We provide experimental results demonstrating how to apply Mueller matrix data to elastography, using chicken liver and chicken heart as biological samples. Finally, we show how the histograms of linear retardance images can he used to distinguish between specimens and quantify the discrimination accuracy. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement