Article
Immunology
Ilandarage Menu Neelaka Molagoda, Wisurumuni Arachchilage Hasitha Maduranga Karunarathne, Mi-Hwa Lee, Chang-Hee Kang, Kyoung Tae Lee, Yung Hyun Choi, Seunghun Lee, Gi-Young Kim
Summary: Acertannin (ACTN), a polyphenol with powerful anticancer and antioxidant effects, has been found to have anti-inflammatory effects. ACTN attenuates proinflammatory cytokines and mediators by inhibiting NF-Kappa B activation, reduces ROS production, and disrupts the binding of LPS to the TLR4/MD2 complex. It also inhibits the recruitment of intracellular adaptor proteins and attenuates NF-Kappa B-mediated inflammatory responses.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Kuo Shen, Yanhui Jia, Xujie Wang, Jinxin Zhang, Kaituo Liu, Jing Wang, Weixia Cai, Jin Li, Shaohui Li, Ming Zhao, Yunchuan Wang, Dahai Hu
Summary: ADSCs exosomes can polarize macrophages to an anti-inflammatory phenotype by regulating the expression of Nrf2 and HO-1, improving inflammation and organ injury in sepsis models.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Immunology
Xiaoxue Bai, Yingjie Zhu, Jing Jie, Dan Li, Lei Song, Jingjing Luo
Summary: This study found that Maackiain, a natural compound from Sophora flavescens, protects against sepsis by reducing organ injury, inflammation, and oxidative stress. Maackiain activates the AMPK/Nrf2/HO-1 pathway, leading to its anti-inflammatory and antioxidant effects. Inhibition of this pathway abolishes the protective effects of Maackiain.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Akash Shivling Mali, Ondrej Honc, Lucie Hejnova, Jiri Novotny
Summary: Opioid receptor agonists have anti-inflammatory and antioxidant effects on microglia, as shown in this study. The selected agonists DAMGO, DADLE, and U-50488 were found to protect microglial cells from lipopolysaccharide-induced oxidative stress by affecting ROS production, NADPH synthesis, and glucose uptake. These beneficial effects were associated with the upregulation of the Nrf2/HO-1 pathway. The findings suggest that opioids can preserve mitochondrial function and eliminate ROS in microglia through a Nrf2/HO-1 signaling pathway-dependent mechanism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Maria Luisa Castejon, Tatiana Montoya, Juan Ortega-Vidal, Joaquin Altarejos, Catalina Alarcon-de-la-Lastra
Summary: The study evaluated the immunomodulatory and anti-inflammatory effects of Ligstroside aglycon (LA) on murine peritoneal macrophages and identified the potential signaling pathways involved. LA showed significant antioxidant and anti-inflammatory effects by reducing oxidative stress markers such as NO production and protein expression. LA was also able to modulate pro-inflammatory cytokine levels and inhibit the activation of certain signaling pathways. The study suggests that LA has potential as a natural agent for the treatment of immune-inflammatory diseases.
Article
Food Science & Technology
Jinyuan Wang, Yinyan Yin, Nuo Xu, Bangyue Zhou, Tao Qin
Summary: The study found that royal jelly could alleviate LPS-induced immune dysfunction of dendritic cells (DCs). It enhanced endocytosis and inhibited allogeneic CD4+ T cell proliferation in LPS-induced DCs. The Nrf2/HO-1 axis played a vital role in this process. These findings suggest the potential application of royal jelly in sepsis therapy.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Biochemistry & Molecular Biology
Yeo Jin Park, Hye Jin Yang, Wei Li, You-Chang Oh, Younghoon Go
Summary: In this study, the effects of Menthae Herba (MH) on the inflammatory response and molecular mechanisms in activated microglia were evaluated. The results showed that MH inhibited the production of proinflammatory enzymes and mediators, reduced reactive oxygen species (ROS) levels, and activated antioxidant signaling pathways. These findings suggest the potential therapeutic use of MH in neuroinflammatory diseases.
Article
Biochemistry & Molecular Biology
Ruiran Wei, Yueqin Zhao, Juan Wang, Xu Yang, Shunlin Li, Yinyuan Wang, Xingzhi Yang, Jimin Fei, Xiaojiang Hao, Yuhan Zhao, Liming Gui, Xiao Ding
Summary: Colorectal cancer (CRC) is a common malignant tumor and traditional treatments have limited efficacy. Ferroptosis, a form of regulated cell death dependent on iron and reactive oxygen species (ROS), has been studied as a promising way to fight against resistant cancers. This study explored the mechanism of action of tagitinin C (TC), a natural product, as a novel ferroptosis inducer in CRC cells, showing that TC induces ferroptosis through ER stress-mediated activation of the PERK-Nrf2-HO-1 signaling pathway.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Dentistry, Oral Surgery & Medicine
Yujun Jiang, Panpan Yang, Congshan Li, Yupu Lu, Yuying Kou, Hongrui Liu, Jie Guo, Minqi Li
Summary: This research investigated the role of periostin in periodontitis and its underlying mechanism. The results showed that decreased periostin expression was associated with periodontitis, and overexpression of periostin suppressed cell apoptosis and reversed the inhibitory effect of lipopolysaccharide on Nrf2 expression. This suggests that periostin could be a potential therapeutic target for periodontitis.
Article
Immunology
Chathuri Kaushalya Marasinghe, Won-Kyo Jung, Jae-Young Je
Summary: This study found that phloroglucinol, a secondary metabolite of marine seaweeds, has potential anti-inflammatory effects by inhibiting oxidative stress, reducing pro-inflammatory cytokine secretion and prostaglandin production in LPS-stimulated RAW264.7 macrophages. These effects may be mediated through the activation of the AMPK/Nrf2/HO-1 pathway.
IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
(2023)
Article
Immunology
Bojian Ye, Dewei He, Jinping Hu, Shuo Yang, Xiyu Gao, Mingchi Cui, Zhe Li, Hefei Wang, Bingxu Huang, Shoupeng Fu, Dianfeng Liu
Summary: Accumulating research has shown that excessive activation of microglia can lead to neuroinflammation and neurodegenerative diseases. This study investigated the effects of a compound called notopterol (NOT) on neuroinflammation and its underlying mechanisms. The findings demonstrated that NOT reduced the expression of pro-inflammatory mediators in cells exposed to LPS and activated the AKT/Nrf2/HO-1 signaling pathway. The anti-inflammatory effect of NOT was inhibited by specific inhibitors of AKT, Nrf2, and HO-1. Furthermore, NOT was able to protect BV-2 cells from LPS-induced damage and increase their survival rate. These results suggest that NOT inhibits the inflammatory response and exerts a neuroprotective effect by modulating the AKT/Nrf2/HO-1 signaling axis in microglia.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Food Science & Technology
Tian-hao Xu, Bing-hao Lin, Cheng-bin Huang, Jing-yu Sun, Kai Tan, Run-xun Ma, Yi-xun Huang, She-ji Weng, Wen-lai Fang, Wei-kai Chen, Bing-li Bai
Summary: In this study, Corynoline was found to have antioxidative and anti-inflammatory properties and it reduced oxidative damage to osteoblasts by activating the Nrf2/HO-1 signaling pathway. These findings provide new insights into the treatment of osteoporosis induced by oxidative injury.
FOOD SCIENCE & NUTRITION
(2023)
Article
Cell Biology
Hanhui Zhong, Haitao Lin, Qiongni Pang, Jinling Zhuang, Xiaolei Liu, Xiaolian Li, Jinghua Liu, Jing Tang
Summary: In this study, it was found that LPS can stimulate macrophages to increase both the protein and cell surface expression of ICAM-1, which is associated with enhanced phagocytosis. Neutralizing antibody or silencing of ICAM-1 can decrease LPS-induced macrophage phagocytosis. Knocking out TLR4 inhibits ROS production, leading to reduced ICAM-1 expression. This study sheds light on the role of macrophage ICAM-1 in endotoxemia through TLR4-mediated ROS production.
INFLAMMATION RESEARCH
(2021)
Article
Medicine, Research & Experimental
Lianxiang Luo, Fangfang Huang, Saiyi Zhong, Rui Ding, Jiating Su, Xiaoling Li
Summary: In this study, it was found that astaxanthin can inhibit inflammation and ferroptosis by regulating the Keap1-Nrf2/HO-1 pathway, thereby reducing the occurrence and development of LPS-induced ALI.
Article
Medicine, Research & Experimental
Jiucui Li, Kongmiao Lu, Fenglan Sun, Shanjuan Tan, Xiao Zhang, Wei Sheng, Wanming Hao, Min Liu, Weihong Lv, Wei Han
Summary: Panaxydol (PX) isolated from Panax ginseng roots attenuates ferroptosis against LPS-induced ALI via the Keap1-Nrf2/HO-1 pathway, showing promising therapeutic potential for ALI treatment.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Alejandro Gonzalez-Benjumea, Gisela Marques, Owik M. Herold-Majumdar, Jan Kiebist, Katrin Scheibner, Jose C. del Rio, Angel T. Martinez, Ana Gutierrez
Summary: Enzymatic conversion of vegetable oils into epoxide derivatives shows potential for industrial applications, with different peroxygenases demonstrating varying oxygenation selectivities. The enzymes exhibit high selectivity towards epoxidation, forming pure epoxides, hydroxylated derivatives, and triepoxides depending on the specific fatty acids present in the oils. This environmentally friendly approach offers promising opportunities for the production of reactive fatty-acid epoxides.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Carmen Aranda, Juan Carro, Alejandro Gonzalez-Benjumea, Esteban D. Babot, Andres Olmedo, Dolores Linde, Angel T. Martinez, Ana Gutierrez
Summary: Selective oxyfunctionalizations of aliphatic compounds are challenging chemical reactions, but enzymes such as P450 monooxygenases and unspecific peroxygenases (UPOs) play important roles due to their stereo- and regio-selectivity. UPOs, discovered in fungi, have emerged as promising biocatalysts for their ability to catalyze the oxyfunctionalization of aliphatic and aromatic compounds using only H2O2.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Biochemistry & Molecular Biology
Tatiana Montoya, Marina Sanchez-Hidalgo, Maria Luisa Castejon, Maria Angeles Rosillo, Alejandro Gonzalez-Benjumea, Catalina Alarcon-de-la-Lastra
Summary: The study demonstrates that dietary supplementation of Oleocanthal can prevent collagen-induced arthritis in a murine model by reducing inflammation and providing protective antioxidant effects. Oleocanthal regulates the production of inflammatory and oxidative mediators, potentially serving as a novel dietary tool for preventing autoimmune and inflammatory disorders such as rheumatoid arthritis.
Review
Nutrition & Dietetics
Tatiana Montoya, Maria Luisa Castejon, Rocio Munoz-Garcia, Catalina Alarcon-de-la-Lastra
Summary: Epigenetic modifications play a crucial role in the development and predisposition of autoimmune diseases, such as systemic lupus erythematosus (SLE). Environmental factors can affect gene expression through epigenetic mechanisms, and diet is one of the most important factors that can influence these alterations. Understanding the cross-link between epigenetic mechanisms, SLE, and dietary factors may lead to novel personalized therapies for SLE patients.
NUTRITION RESEARCH REVIEWS
(2023)
Article
Cell Biology
Marina Aparicio-Soto, Caterina Curato, Franziska Riedel, Hermann-Josef Thierse, Andreas Luch, Katherina Siewert
Summary: This article systematically reviews research papers on T cell activation to chemical skin sensitizers published in the past 20 years. The results show current challenges and possible solutions in monitoring and predicting T cell responses, and provide guidance for the further development of T cell assays, including cross-reactivity assessments.
Article
Biochemistry & Molecular Biology
Tatiana Montoya, Catalina Alarcon-de-la-Lastra, Maria Luisa Castejon, Juan Ortega-Vidal, Joaquin Altarejos, Marina Sanchez-Hidalgo
Summary: This study explores the antioxidant and anti-inflammatory effects of (-)-methyl-oleocanthal (met-OLE), a metabolite of extra virgin olive oil (EVOO), in murine peritoneal macrophages. It reveals that met-OLE inhibits the production of reactive oxygen species and pro-inflammatory enzymes, while upregulating anti-inflammatory markers. The study also suggests that met-OLE may contribute to the beneficial effects associated with EVOO consumption through epigenetic changes.
Article
Biochemistry & Molecular Biology
Dolores Linde, Elena Santillana, Elena Fernandez-Fueyo, Alejandro Gonzalez-Benjumea, Juan Carro, Ana Gutierrez, Angel T. Martinez, Antonio Romero
Summary: This study successfully crystallized two UPO enzymes, rMroUPO and rCviUPO, from different fungal groups through sequence optimization and Escherichia coli expression, revealing their crystal structures and dimeric protein characteristics.
Article
Biochemistry & Molecular Biology
Dolores Linde, Alejandro Gonzalez-Benjumea, Carmen Aranda, Juan Carro, Ana Gutierrez, Angel T. Martinez
Summary: Vegetable oils are valuable renewable resources for the production of bio-based chemicals and intermediates. Enzymes can be used as environmentally friendly alternatives to chemical catalysis in the epoxidation reactions of vegetable oils. By engineering the active site of the enzyme and optimizing the process, the epoxidation yield can be significantly enhanced.
Editorial Material
Biochemistry & Molecular Biology
Catalina Alarcon-de-la-Lastra
Article
Chemistry, Medicinal
Manuel Alcarranza, Isabel Villegas, Rocio Munoz-Garcia, Rocio Recio, Inmaculada Fernandez, Catalina Alarcon-de-la-Lastra
Summary: This study found that the natural enantiomer (R)-Sulforaphane (SFN) could modulate the immune response in LPS-stimulated murine macrophages and spleen cells by inhibiting oxidative stress and inflammatory factors. The effects of (R)-SFN on immune modulation may be mediated through various signaling pathways and epigenetic modifications. These findings suggest that (R)-SFN could be a promising compound for the management of immunoinflammatory diseases.
Article
Allergy
Franziska Riedel, Marina Aparicio-Soto, Caterina Curato, Lucas Muench, Amro Abbas, Hermann-Josef Thierse, Wiebke K. Peitsch, Andreas Luch, Katherina Siewert
Summary: In this study, a CD154 upregulation assay was used to quantify Ni2+, Co2+, and Pd2+ specific CD4+ T cells, and the related TCR repertoire was analyzed by high-throughput sequencing. The results revealed an overexpression of the gene segment TRAV9-2 in Ni2+ specific TCRs, while Co2+ and Pd2+ specific TCRs favored the expression of other gene segments. Furthermore, a histidine in the CDR3 of metal-specific TCRs was found to be conserved among cross-reactive clonotypes.
Article
Food Science & Technology
Tatiana Montoya, Marina Sanchez-Hidalgo, Maria Luisa Castejon, Maria Victoria Vazquez-Roman, Maria Alvarez de Sotomayor, Juan Ortega-Vidal, Maria Luisa Gonzalez, Catalina Alarcon-de-la-Lastra
Summary: This study aimed to evaluate the effects of OLE enriched diet on renal damage and aortic endothelial dysfunction in murine pristane-induced SLE. The results showed that dietary OLE supplementation reduced pro-inflammatory cytokines production, alleviated renal damage, and improved aortic endothelial dysfunction by regulating multiple signaling pathways. These preliminary results suggest OLE as a new therapeutic strategy in SLE management.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Maria Luisa Castejon, Tatiana Montoya, Juan Ortega-Vidal, Joaquin Altarejos, Catalina Alarcon-de-la-Lastra
Summary: The study evaluated the immunomodulatory and anti-inflammatory effects of Ligstroside aglycon (LA) on murine peritoneal macrophages and identified the potential signaling pathways involved. LA showed significant antioxidant and anti-inflammatory effects by reducing oxidative stress markers such as NO production and protein expression. LA was also able to modulate pro-inflammatory cytokine levels and inhibit the activation of certain signaling pathways. The study suggests that LA has potential as a natural agent for the treatment of immune-inflammatory diseases.
Meeting Abstract
Orthopedics
M. S. Meiss, K. M. Villagran-Andrade, P. C. Calder, J. G. Fernandez-Bolanos, C. Alarcon-de-la-Lastra, M. Sanchez-Hidalgo, F. J. Blanco, R. O. Oreffo, M. C. de Andres
OSTEOARTHRITIS AND CARTILAGE
(2021)
