期刊
ULTRAMICROSCOPY
卷 201, 期 -, 页码 58-67出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ultramic.2019.03.002
关键词
Ultramicrotomy; Multiscale imaging; Pre-screening; 3View SBF-SEM; IMOD
类别
资金
- Engineering and Physical Science Research Council (EPSRC) within the Henry Royce Institute [EP/F007906/1, EP/F001452/1, EP/I02249X, EP/F028431/1, EP/M022498/1, EP/M010619/1]
- European Research Council [695638 CORREL-CT]
- Wellcome Trust [203128/Z/16/Z, WT100290MA]
- EPSRC [EP/M010619/1, EP/F001452/1, EP/M022498/1, EP/I02249X/1, EP/F028431/1, EP/F007906/1] Funding Source: UKRI
Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) can provide unrivalled high-resolution images of specific features and volumes of interest. However, the regions interrogated are typically very small, and sample preparation is both time-consuming and destructive. Here we consider how prior X-ray micro-computed tomography (microCT) presents an opportunity to increase the efficiency of electron microscopy in biology. We demonstrate how it can be used to; select the most promising samples and target site-specific locations; provide a wider context of the location being interrogated (multiscale correlative imaging); guide sample preparation and 3D imaging schemes; as well as quantify the effects of destructive sample preparation and staining procedures. We present a workflow utilising open source software in which microCT can be used either broadly, or precisely, to experimentally steer and inform subsequent electron microscopy studies. As automated sample registration procedures are developed to enable correlative microscopy, experimental steering by prior CT could be beneficially routinely incorporated into many experimental workflows.
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