Review
Biotechnology & Applied Microbiology
Lucie A. Low, Christine Mummery, Brian R. Berridge, Christopher P. Austin, Danilo A. Tagle
Summary: OoCs, also known as microphysiological systems or 'tissue chips', have the potential to be informative at multiple stages of drug discovery and development, providing insights into normal human organ function and disease pathophysiology, as well as predicting the safety and efficacy of investigational drugs in humans. The field of OoCs has seen significant advances in recent years, but there are still challenges and opportunities that need to be addressed to fully realize their potential for translational research.
NATURE REVIEWS DRUG DISCOVERY
(2021)
Editorial Material
Biotechnology & Applied Microbiology
Carly Strelez, Hannah Y. Jiang, Shannon M. Mumenthaler
Summary: Recreating "living organs" using groundbreaking organ-on-a-chip (OOC) technologies is revolutionizing drug discovery. Studies by Huh et al. and Ronaldson-Bouchard et al. highlight the advancements made over the past decade, from single organ functionality to interconnected organs, allowing for the examination of drug toxicities and disease pathogenesis in reconstituted tissues.
TRENDS IN BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Mees N. S. de Graaf, Aisen Vivas, Andries D. van der Meer, Christine L. Mummery, Valeria V. Orlova
Summary: Organ-on-chip devices are used to simulate the tissue microenvironment of cells in intact organs. This study presents a method for multiplexing microfluidic channels in these devices, along with custom software for system operation. The approach is also suitable for culture medium recirculation, which is important for cost considerations.
Review
Pharmacology & Pharmacy
Zohreh Izadifar, Alexandra Sontheimer-Phelps, Bob A. Lubamba, Haiqing Bai, Cicely Fadel, Anna Stejskalova, Alican Ozkan, Queeny Dasgupta, Amir Bein, Abidemi Junaid, Aakanksha Gulati, Gautam Mahajan, Seongmin Kim, Nina T. LoGrande, Arash Naziripour, Donald E. Ingber
Summary: Studying mucus biology has been challenging due to the lack of physiologically relevant human in vitro models. However, recent progress has been made in the development of human organ-on-a-chip microfluidic culture models, focusing on the lung, colon, small intestine, cervix, and vagina. These models can reconstitute epithelial tissue barriers and physiologically relevant mucus layers, allowing for the study of mucus composition, mechanics, and structure in a biomimetic environment.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Review
Chemistry, Multidisciplinary
Lingyu Sun, Feika Bian, Dongyu Xu, Yuan Luo, Yongan Wang, Yuanjin Zhao
Summary: This article provides an overview of the advances in biomaterials for the construction of organs-on-chips. It introduces the composition, structure, and fabrication techniques of biomaterials, and focuses on their functions and applications in organs-on-chips systems. The use of biomaterial-based organs-on-chips as alternative animal models and the challenges and prospects of further functionalization are also discussed.
MATERIALS HORIZONS
(2023)
Review
Chemistry, Multidisciplinary
Yaqing Wang, Peng Wang, Jianhua Qin
Summary: This review introduces two cutting-edge technologies, stem cell organoids and bioengineered organs-on-chips, which can construct biomimetic three-dimensional tissue or organ models that closely mimic human responses. It highlights the recent progress and potential utility of these models in virology research, particularly in addressing the COVID-19 pandemic and developing vaccines and therapies. It also discusses the future opportunities and challenges in the development of advanced human organ models for translational applications.
Review
Microbiology
Jens Puschhof, Cayetano Pleguezuelos-Manzano, Hans Clevers
Summary: In vitro co-culture models are important tools for studying interactions between microbiota and intestinal tissues. These models can replicate specific processes of microbe-epithelia interactions. By using different models of bacterial, viral, and parasitic infections, the advantages of each culture model can be highlighted.
CELL HOST & MICROBE
(2021)
Review
Biotechnology & Applied Microbiology
Yi Yang, Yin Chen, Liang Wang, Shihui Xu, Guoqing Fang, Xilin Guo, Zaozao Chen, Zhongze Gu
Summary: The combination of organ-on-a-chip technology and physiologically based pharmacokinetic modeling has the potential to improve drug development and personalized medicine.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Lisette Van Os, Britta Engelhardt, Olivier T. Guenat
Summary: In order to develop new therapies for infections, understanding the human immune system during infection is crucial. Organ-on-chip models have proven to be valuable tools for tissue modeling, and incorporating an immune component is necessary to simulate complex biological responses. This tutorial review provides a practical guide on developing an organ-on-chip model to investigate immune cell migration during infection, including the design of the chip, establishment of chemotactic gradients, and incorporation of different cell types. The review also highlights the importance of the extracellular matrix in accurately modeling the interstitial space for immune cell migration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Engineering, Biomedical
Hongjian Zhang, Chengtie Wu
Summary: Neurovascular networks play important roles in tissue and organ regeneration. Traditional scaffolds cannot meet the requirement of angiogenesis and innervation, but 3D printing provides a versatile technique to fabricate biomimetic scaffolds. This review summarizes the progress in using 3D-printed biomaterials for vascularized and innervated tissue regeneration.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Chemistry, Multidisciplinary
Fangyu Qiao, Yang Zou, Binglin Bie, Yonggang Lv
Summary: Vascularization and innervation play crucial roles in bone regeneration and repair. This study presents a genetically functionalized matrix (GFM) that regulates the expression of vascular and neural related genes, promoting angiogenesis and neurogenesis to enhance bone defect repair.
Review
Cell Biology
Louis Jun Ye Ong, Xiwei Fan, Antonia Rujia Sun, Lin Mei, Yi-Chin Toh, Indira Prasadam
Summary: Osteoarthritis (OA) is a prevalent disease worldwide, affecting over 20% of the population and leading to decreased quality of life for patients. Due to the complexity of mimicking joint tissue's physiological environment, the study of OA pathophysiology primarily relies on animal models. However, recent advancements in microfluidic organ-on-chip (OoC) systems have shown promise in replicating and manipulating tissue physiological environments. By adapting these techniques, it is possible to create human-specific in vitro models that accurately capture the cellular processes involved in OA. This review provides a comprehensive summary of various microfluidic platforms used to mimic joint microenvironments, with implications for future platform design iterations.
Article
Engineering, Biomedical
Chen Qin, Hongjian Zhang, Lei Chen, Meng Zhang, Jingge Ma, Hui Zhuang, Zhiguang Huan, Yin Xiao, Chengtie Wu
Summary: To achieve regeneration of highly vascularized and innervated tissues, a pre-angiogenic cell-laden scaffold with durable angiogenic functions is prepared. The scaffold supports long-term cell survival and growth, and exhibits durable angiogenic capability both in vitro and in vivo. The pre-angiogenic scaffolds can induce the neurogenetic differentiation and osteogenic differentiation, and may represent the future direction of biomaterials for complex tissue/organ regeneration.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Hongjian Zhang, Meng Zhang, Dong Zhai, Chen Qin, Yufeng Wang, Jingge Ma, Hui Zhuang, Zhe Shi, Liang Wang, Chengtie Wu
Summary: Inspired by space-filling polyhedra, polyhedron-like scaffolds with spatial topologies are prepared via 3D-printing technology, and they significantly promote osteogenesis, angiogenesis, and neurogenesis in vitro, as well as inducing innervated and vascularized bone regeneration in vivo.
ADVANCED MATERIALS
(2023)
Review
Pharmacology & Pharmacy
Berivan Cecen, Christina Karavasili, Mubashir Nazir, Anant Bhusal, Elvan Dogan, Fatemeh Shahriyari, Sedef Tamburaci, Melda Buyukoz, Leyla Didem Kozaci, Amir K. Miri
Summary: Organ-on-a-chip technology is utilized for testing small-molecule drugs, with a focus on simulating interactions between drugs and the body. The development of multi-organ-on-a-chip (MOC) technology aims to replicate various organs involved in drug-body interactions. Current research in MOCs is particularly concentrated on studying the dynamic processes of drug absorption, distribution, metabolism, and excretion.