Article
Plant Sciences
Sahar Mehrpouyan, Usha Menon, Ian J. Tetlow, Michael J. Emes
Summary: This study focuses on the post-translational modification of SSIIa and its interactions with SSI and SBEIIb in maize amyloplast stroma, revealing that phosphorylation can promote the interaction between SSIIa and SBEIIb to enhance its activity. Protein phosphorylation plays a dual role in the stability and dissociation of heteromeric starch synthase complexes in the maize amyloplast stroma, providing new insights into the regulation of starch biosynthesis in plants.
Article
Food Science & Technology
Elsa Gutierrez-Cortez, Ezequiel Hernandez-Becerra, Sandra M. Londono-Restrepo, Beatriz M. Millan-Malo, Eduardo Morales-Sanchez, Marcela Gaytan-Martinez, Mario E. Rodriguez-Garcia
Summary: This study focused on the physiochemical changes in maize endosperm during a thermo-alkaline nixtamalization process. It was found that calcium content increased in the outer layers, starch granules showed no damage, and the pasting properties were influenced by the morphology of the paste.
JOURNAL OF CEREAL SCIENCE
(2022)
Article
Agriculture, Multidisciplinary
Yuyue Zhong, Jianzhou Qu, Andreas Blennow, Xingxun Liu, Dongwei Guo
Summary: The study on the molecular structure and expression levels of starch biosynthesis-related genes in high-amylose maize (HAM) and normal maize (NM) genotypes revealed distinct patterns of starch content and molecular size at different time points after pollination. Correlation analyses indicated that the expression levels of specific genes were associated with the content of starch and the molecular structure of amylopectin in different genotypes.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Chemistry, Applied
Nancy N. Caballero-Rothar, Lucas Borras, Jose A. Gerde
Summary: In this study, it was found that digestibility of maize starch is slower in hard endosperm genotypes, which is related to specific zein concentrations. Cooking with sodium sulfite can significantly increase starch hydrolysis, whereas the ratio of amylose to starch does not impact starch digestibility.
Article
Biochemistry & Molecular Biology
Yongyan Wang, Dongsheng Shi, Hui Zhu, Hanxue Yin, Gaoyang Wang, Anqi Yang, Zhixuan Song, Qingquan Jing, Bilian Shuai, Ningkun Xu, Jianping Yang, Hongyu Chen, Guifeng Wang
Summary: Rerouting the starch biosynthesis pathway in maize can generate specialty types, like sweet corn and waxy corn. A new maize mutant, bt1774, with decreased starch content and increased soluble sugars at maturity was characterized. The BRITTLE ENDOSPERM2 (Bt2) gene, encoding a subunit of ADP-glucose pyrophosphorylase (AGPase), was found to be the causal gene for bt1774. Bt2 is likely involved in carbohydrate flux and balance, regulating both the BETL development and the starchy endosperm filling.
Article
Biochemistry & Molecular Biology
Naoko Crofts, Yoshiki Satoh, Satoko Miura, Yuko Hosaka, Misato Abe, Naoko Fujita
Summary: The introduction of higher SSIIa activity effectively restores crystallinity, starch granule structure, and production of plump seeds in the mild-type sug-1 mutant. Crossbreeding resulted in the generation of new rice lines with improved amylopectin-like glucan accumulation and A-type crystallinity, complementing the wrinkled seed phenotype typical of the original isa1 mutation.
PLANT MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xiaojian Peng, Wei Yu, Yirong Chen, Yingli Jiang, Yaru Ji, Long Chen, Beijiu Cheng, Jiandong Wu
Summary: In this study, a novel starch regulatory gene, ZmCBM48-1, was identified, which was found to play a positive regulatory role in the starch synthesis pathway by up-regulating several starch synthesis-related genes. Furthermore, its overexpression in rice led to changes in the endosperm cell shape and starch structure of transgenic seeds.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jihui Zhu, Chang-Quan Zhang, Jianlong Xu, Robert G. Gilbert, Qiaoquan Liu
Summary: The study explores the impact of starch synthase isoforms on chain-length distributions of starch in rice, identifying the enzyme sets controlling amylose and amylopectin biosynthesis and the mutants of each isoform. This detailed analysis enables breeders to target and develop improved rice species based on functional properties controlled by CLD.
Article
Plant Sciences
Christina Finegan, Susan K. Boehlein, Kristen A. Leach, Gabriela Madrid, L. Curtis Hannah, Karen E. Koch, William F. Tracy, Marcio F. R. Resende
Summary: In this study, maize mutants were used to investigate the key genes and regulatory mechanisms involved in endosperm starch biosynthesis. By performing differential expression and coexpression network analysis, potential regulators of starch mutations were identified. The results also shed light on the mechanisms of certain genes and their roles in starch biosynthesis and grain filling.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Tina B. Schreier, Brendan Fahy, Laure C. David, Hamad Siddiqui, Roger Castells-Graells, Alison M. Smith
Summary: Expressing a bacterial glucan synthase in wheat grains led to the accumulation of high levels of maltose, causing swelling during development and shrivelling upon maturity. Cytosolic glucan synthesized was hydrolyzed immediately to maltose, attracting excess water and stretching the seed coat before shrinking during grain maturation.
Article
Plant Sciences
Long Chen, Ming Du, Long Wang, Wei Yu, Yirong Chen, Beijiu Cheng, Jiandong Wu
Summary: This study identified a novel maize starch synthesis regulating protein, ZmSSRP1, and demonstrated its potential role in regulating starch synthesis. Overexpression of ZmSSRP1 in rice resulted in changes in grain quality and starch structure, providing insights for molecular breeding of high-yielding high-quality maize.
FUNCTIONAL PLANT BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Dawei Dai, Zeyang Ma, Rentao Song
Summary: Recent breakthroughs in transcriptome analysis and gene characterization have provided valuable insights into the genetic control of maize endosperm development. Specific genes and transcriptional regulatory networks have been identified to understand the molecular factors involved in different compartments of endosperm development.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hai-Teng Li, Wenyu Zhang, Wenwen Pan, Yangyang Chen, Yulong Bao, Alexander T. Bui
Summary: Limited knowledge exists on the regulation of molecular composition of starch leachates through irradiative depolymerization. This study investigated the leaching behavior of electron beam depolymerized maize starch and its relationship with modified starch granule structures. The findings showed a decrease in branching degree and the presence of a smaller amylopectin fraction in the leached starch, as well as changes in the granule properties. Principal component and Pearson correlation analysis revealed that the degree of branching and the newly-derived amylopectin fraction significantly correlated with the molecular size of leached starch molecules. The study suggests that cleavage of alpha-1,6 linkage plays a critical role in controlling the leaching process of irradiated starch granules, offering potential for optimizing starch-based food properties.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Biochemistry & Molecular Biology
Yasunori Nakamura
Summary: Amylopectin biosynthesis involves the reproduction of a new cluster by concerted actions of multiple isoforms of starch branching enzyme (BE). Among these isoforms, BEI plays a major role in initiating the formation of a new cluster due to its broad chain-length preference. On the other hand, BEIIb is unlikely to be involved in this reaction due to its preference for short chains. BEIIa may complement the role of BEI to some extent. The model also suggests that the first branches mainly formed by BEI contribute to the amorphous lamellae, while the second branches predominantly formed by BEIIb are located in the crystalline lamellae.
PLANT MOLECULAR BIOLOGY
(2023)
Article
Plant Sciences
Babatope Samuel Ajayo, Yangping Li, Yayun Wang, Chengdong Dai, Lei Gao, Hanmei Liu, Guowu Yu, Junjie Zhang, Yubi Huang, Yufeng Hu
Summary: This study identifies ZmTCP7 as a transcriptional repressor of ZmBt2 and a negative regulator of endosperm starch accumulation in maize. The findings provide new insights into the regulatory networks governing ZmBt2 expression and starch biosynthesis pathway in maize.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Mark E. Larson, Daniel J. Falconer, Alan M. Myers, Adam W. Barb
JOURNAL OF BIOLOGICAL CHEMISTRY
(2016)
Article
Multidisciplinary Sciences
Ryan R. Lappe, John W. Baier, Susan K. Boehlein, Ryan Huffman, Qiaohui Lin, Fabrice Wattebled, A. Mark Settles, L. Curtis Hannah, Ljudmilla Borisjuk, Hardy Rolletschek, Jon D. Stewart, M. Paul Scott, Tracie A. Hennen-Bierwagen, Alan M. Myers
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Agriculture, Multidisciplinary
Fan Zhu, Eric Bertoft, Anna Kallman, Alan M. Myers, Koushik Seetharaman
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2013)
Article
Microbiology
Alexandra R. Volker, David S. Gogerty, Christian Bartholomay, Tracie Hennen-Bierwagen, Huilin Zhu, Thomas A. Bobik
Article
Plant Sciences
Qiaohui Lin, Maud Facon, Jean-Luc Putaux, Jason R. Dinges, Fabrice Wattebled, Christophe D'Hulst, Tracie A. Hennen-Bierwagen, Alan M. Myers
Article
Plant Sciences
Binquan Huang, Tracie A. Hennen-Bierwagen, Alan M. Myers
Article
Multidisciplinary Sciences
Hao Wu, Kasi Clay, Stephanie S. Thompson, Tracie A. Hennen-Bierwagen, Bethany J. Andrews, Bernd Zechmann, Bryan C. Gibbon
Article
Plant Sciences
Ying Xie, Adam W. Barb, Tracie A. Hennen-Bierwagen, Alan M. Myers
FRONTIERS IN PLANT SCIENCE
(2018)
Article
Cell Biology
Junya Zhang, Shan Wu, Susan K. Boehlein, Donald R. McCarty, Gaoyuan Song, Justin W. Walley, Man Myers, A. Mark Settles
JOURNAL OF CELL BIOLOGY
(2019)
Article
Multidisciplinary Sciences
Camila Ribeiro, Tracie A. Hennen-Bierwagen, Alan M. Myers, Kenneth Cline, A. Mark Settles
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Biochemistry & Molecular Biology
Yuri Nagaoka, Kotone Oshiro, Yuta Yoshino, Toshiyuki Matsunaga, Satoshi Endo, Akira Ikari
Summary: This study investigated the effect of intercellular adhesion molecule CLDN1 on the anticancer drug sensitivity of small-cell lung cancer (SCLC) cells. It was found that overexpression of CLDN1 decreased the sensitivity of SCLC cells to anticancer drugs and enhanced their migratory capacity through the activation of the TGF-81/EMT signaling pathway. Treatment with EMT inhibitors showed potential in overcoming the reduced sensitivity to anticancer drugs in CLDN1-overexpressing SCLC cells.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2024)