期刊
TALANTA
卷 198, 期 -, 页码 510-517出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2019.01.109
关键词
Aptasensing; beta-Amyloid((1)(-)(40)); Beta-Amyloid; Amyloid-beta; Electrochemical sensor; Neurodegenerative disease
资金
- Iran National Science Foundation [96005985]
- Research Councils of Shiraz University of Medical Sciences
An extremely sensitive and highly simple aptasensor was fabricated for quantitation of amyloid beta (A beta) by electrochemical transduction of a fern leaves-like gold nanostructure. The gold nanostructure was synthesized by electrodeposition using polyethylene glycol 6000 as a shape-directing agent, and characterized electrochemically and by field emission scanning electron microscopy. A specific RNA aptamer was immobilized on the fern leaves-like gold nanostructure, and binding with A beta was detected by the ferro/ferricyanide redox marker. The designed aptasensor was able to detect A beta in a linear range of 0.002-1.28 ng mL(-1) and a limit of detection of 0.4 pg mL(-1) (88.6 amol L-1). The aptasensor was interference-free, and for demonstration of its viability for AB determination in real samples, the human blood serum and artificial cerebrospinal fluid containing A beta were analyzed. The aptasensor is applicable for the assessment and management of Alzheimer's disease at early stages.
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