Article
Biochemical Research Methods
Huali Jin, Ruike Liu, Tian Bai, Min Wei, Baoshan He, Zhiguang Suo
Summary: A low-noise ratiometric fluorescence biosensor based on Pb2+-dependent DNAzyme and exonuclease III (Exo III)-assisted cascade signal amplification was developed for the detection of lead ions. The biosensor exhibited a lower limit of detection and a wider detection range compared to other methods, and showed satisfactory results in the detection of lead ions in tap water, tea, and rice flour samples. This ratiometric fluorescence biosensor has great potential in various target monitoring.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Ruike Liu, Baoshan He, Huali Jin, Zhiguang Suo
Summary: A fluorescent aptasensor was developed for the detection of lead ion. The sensor exhibited high selectivity and sensitivity, making it suitable for the identification of lead ions in real samples.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Ghasem Anbiaee, Zahra Khoshbin, Hamed Zahraee, Mohammad Ramezani, Mona Alibolandi, Khalil Abnous, Seyed Mohammad Taghdisi
Summary: Developing cost-effective smart sensing tools with ultrahigh sensitivity for food safety insurance is highly important. Aptamer-based biosensors powered by recycling signal amplification strategies, such as target recycling supported by enzymes, have shown great potential in achieving signal amplification. Exonucleases, as superior biocatalysts, simplify the amplification process and exhibit outstanding selectivity and sensitivity, making them promising sensing tools for label-free, ease-of-use, cost-effective, and real-time diagnosis of diverse targets. This article summarizes the achievements and perspectives in aptasensor design for qualitative monitoring of diverse targets using exonucleases for signal amplification.
Article
Chemistry, Analytical
Tingting Hu, Changbei Ma, Ying Yan, Junxiang Chen
Summary: A sensitive method for detecting DNA methyltransferase (MTase) activity was developed using fluorescence resonance energy transfer (FRET) and exonuclease (Exo) III-mediated signal amplification. This method showed high sensitivity with a lowest detection limit of 0.045 U/mL for MTase, and was also suitable for MTase-inhibitor screening.
Article
Chemistry, Analytical
Song Wang, Guangjie Kang, Fangli Cui, Yingwei Zhang
Summary: The study developed a signal off-on highly sensitive multiplex detection platform using dual-color graphene quantum dots and carbon nanoparticles for multiple target DNA detection, incorporating a sequence-independent target recycling and signal amplification strategy, achieving high sensitivity.
ANALYTICA CHIMICA ACTA
(2021)
Article
Food Science & Technology
Yu Chen, Fuyuan Zhang, Ruobing Liu, Minxuan Liu, Yaxin Sang, Shuo Wang, Xianghong Wang
Summary: A novel and sensitive aptasensor for AFB1 was developed using an Exo III-integrated signal amplification strategy. The method achieved a low detection limit, wide detection range, and demonstrated good performance in complex matrices. The recovery rate and RSD in cereal samples were comparable to or better than previously reported methods, showing the potential of this sensing method for practical applications.
Article
Spectroscopy
Boxu Wang, Zheng Liu, Zhihong Li, Ningyi Xu, Xuejiao Zhang, Ruifang Su, Junyang Wang, Rui Jin, Chunyan Sun
Summary: In this study, a fluorescent probe was constructed for facile and sensitive detection of Hg2+ based on the unique fluorescent property of 2-aminopurine (2-AP). The probe exhibited an excellent linear relationship with Hg2+ concentration and had a low detection limit. Satisfactory detection results were obtained in water and fish samples.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Biochemistry & Molecular Biology
Ting Sun, Xian Li, Xiaochuan Jin, Ziyi Wu, Xiachao Chen, Jieqiong Qiu
Summary: Graphene oxide with positive charge (pGO25000) was developed and showed improved fluorescence quenching ability. The affinity between pGO25000 and DNA was increased by additional electrostatic interaction, resulting in almost complete quenching of FAM-labeled single-stranded DNA at low concentrations of pGO25000.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Qirong Chen, Huahui Gao, Jianglong Yao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a new target/aptamer binding-induced inhibition of enzyme activity strategy was used to construct an amplified and sensitive electrochemical biosensor for detecting Sonic Hedgehog (SHh). The sensor exhibited high selectivity and could detect SHh down to 3.34 pM. This strategy has the potential to be a convenient approach for detecting different proteins in biological studies and disease diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Haining Cui, Keqi An, Chengquan Wang, Yangfang Chen, Suli Jia, Jing Qian, Nan Hao, Jie Wei, Kun Wang
Summary: A disposable ratiometric electrochemical aptasensor was constructed for highly sensitive detection of aflatoxin B1. Au nanoflowers on ITO surface were used for DNA anchoring and methylene blue labeling. The detection was based on the peak current intensity ratio (I-MB/I-Fc) as the signal transducer. The aptasensor exhibited a wide detection range and a low detection limit.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Xingyuan Niu, Jie Yang, Zhiguang Suo, Min Wei, Yong Liu, Baoshan He, Huali Jin
Summary: Rapid and sensitive detection of the highly toxic and carcinogenic substance Aflatoxin B1 (AFB1) is essential for food safety and human health. In this study, a fluorescent aptasensor based on T7 exonuclease (T7 EXO) double recycling amplification was developed, using novel nanomaterial carbon nanocages (CNCs) as fluorescence resonance energy transfer (FRET) receptors. The CNCs, with their three-dimensional structure, exhibited higher quenching efficiency than conventional materials due to their larger surface area and strong adsorption capacity. The method achieved a low limit of detection (LOD) of 1.4 pg/mL and showed excellent potential for application in real samples.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Chen-Chen Bai, Di Wang, Ming-Xia Liu, Yi-Rong Ma, Yi Sun, Rui Duan, Lin-Yi Dong, Xian-Hua Wang
Summary: A novel approach utilizing boronic acid-modified graphene oxide and boronate-affinity-oriented magnetic nanoparticles was proposed for ultrasensitive and specific detection of glycoprotein, showing high sensitivity with limit of detections of 23 fg mL-1 and 1.2 fg mL-1 for different proteins. This strategy demonstrated universal applicability and successful application in detecting glycoproteins in spiked serum samples, suggesting potential in clinical diagnosis and prognosis evaluation of glycoprotein related diseases.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Yi Shen, Haoyu Yuan, Zixuan Guo, Xiu-Qing Li, Zhiqing Yang, Chengli Zong
Summary: Exonuclease III (Exo III) is commonly used as a dsDNA-specific exonuclease that doesn't degrade ssDNA. However, this study demonstrates that Exo III at concentrations above 0.1 unit/μL can efficiently digest linear ssDNA. Additionally, the dsDNA specificity of Exo III is crucial in DNA target recycling amplification (TRA) assays. The findings expand the substrate scope of Exo III and have implications for its experimental applications.
Article
Chemistry, Analytical
Yang Hu, Hanyin Xie, Jiaying Hu, Danting Yang
Summary: This study developed a novel biosensor based on disposable screen-printed carbon electrodes (SPCEs) for the ultrasensitive detection of ochratoxin A (OTA). Graphene oxide-DNA (GO-DNA) complex as a signal amplifier was investigated for the first time. The proposed aptasensor showed a low limit of detection and good reproducibility, and the OTA detection result was comparable to that of the ELISA kit.
Article
Chemistry, Analytical
Luyang Zhao, Zhiguang Suo, Baoshan He, Yawei Huang, Yong Liu, Min Wei, Huali Jin
Summary: A novel nitrogen-doped carbon supported palladium material (C-N-Pd) was synthesized and used in constructing a fluorescent aptasensor for AFB1 detection. The aptasensor exhibited high sensitivity and good selectivity towards AFB1, and successfully detected the toxin in contaminated food samples. The aptasensor showed good agreement with the HPLC method, indicating its potential as a reliable detection tool for AFB1.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).