期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 224, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2019.117435
关键词
Benzimidazole; 7-Hydroxycoumarin; Fluorescent probe; Two-photon; Mitochondrial pH
类别
资金
- National Natural Science Foundation [81672458]
- Science and Technology Planning Project of Sichuan Province [2016JY0101, 2018JY0237, 2019JDPT0010]
- Educational Commission of Sichuan Province [18ZA0528, 18TD0051]
- Health Commission of Sichuan Province [18PJ019]
- Luzhou City [2016LZXNYD-T03, 2018LZXNYD-ZK18, 2018LZXNYD-ZK28]
- Southwest Medical University [2016LZXNYD-T03, 2018LZXNYD-ZK18, 2018LZXNYD-ZK28]
- Talent Introduction Startup Fund of Affiliated Hospital of Southwest Medical University [18052, 18054]
- Open Program of Nuclear Medicine and Molecular Imaging Key Laboratory of Sichuan Province [HYX18010, HYX18002]
A novel two-photon pH probe, 3-benzimidazole-7-hydroxycoumarin (BHC), was designed and synthesized based on the structures of hydroxycoumarin and benzimidazole. BHC showed good linearity in the pH ranges of 3.30-5.40 (pKa = 4.20) and 6.50-8.30 (pKa = 7.20) at a maximum emission wavelength of 480 nm. BHC in acidic and alkaline media could be distinguished by an obvious spectral shift of the maximum absorption wavelength from 390 nm to 420 nm. In addition, BHC was well localized to mitochondria and successfully applied to one-photon and two-photon imaging of pH changes in the mitochondria of HeLa cells. The findings presented herein suggest that BHC can serve as an excellent fluorescent probe for selectively sensing mitochondrial pH changes with remarkable photostability and low cytotoxicity. (C) 2019 Elsevier B.V. All rights reserved.
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