期刊
RNA BIOLOGY
卷 16, 期 10, 页码 1377-1385出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15476286.2019.1630797
关键词
RNA structure; RNA methylation; epigenetic modification; N-6-methyladenine; kink-turn; X-ray crystallography
资金
- Cancer Research UK [A18604]
- EPSRC [EP/J017094/1] Funding Source: UKRI
N-6-methyladenine is the most common covalent modification in cellular RNA species, with demonstrated functional consequences. At the molecular level this methylation could alter local RNA structure, and/or modulate the binding of specific proteins. We have previously shown that trans-Hoogsteen-sugar (sheared) A:G base pairs can be completely disrupted by methylation, and that this occurs in a sub-set ofD/D k-turn structures. In this work we have investigated to what extent sequence context affects the severity with which inclusion of N-6-methyladenine into different A:G base pairs of a standard k-turn affects RNA folding and L7Ae protein binding. We find that local sequence has a major influence, ranging from complete absence of folding and protein binding to a relatively mild effect. We have determined the crystal structure of one of these species both free and protein-bound, showing the environment of the methyl group and the way the modification is accommodated into the k-turn structure.
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