Article
Biophysics
Yongan Ren, Ke Ge, Danyang Sun, Zichen Hong, Changku Jia, Huan Hu, Fangwei Shao, Bo Yao
Summary: A strategy and technology were proposed to assess the lipid amount and membrane protein expression of extracellular vesicles, along with the development of a microfluidic platform for effective EVs enrichment and detection.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biochemistry & Molecular Biology
Julia Neumair, Claudia D'Ercole, Matteo De March, Martin Elsner, Michael Seidel, Ario de Marco
Summary: In this study, a new affinity chromatography platform composed of a macroporous epoxy monolith matrix functionalized with anti-CD63 nanobodies was developed. The platform successfully captured and released integral EVs from urine samples with negligible unspecific binding for circulating proteins. Size discrimination of eluted EVs was achieved by different elution approaches. The use of inexpensive recombinant nanobodies and scalable monolith materials make this platform feasible and affordable for large-scale production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Rufus Vinod, Randa Mahran, Erica Routila, Janne Leivo, Kim Pettersson, Kamlesh Gidwani
Summary: The study introduces a new assay using europium-nanoparticle to detect and characterize different glycoconjugates on the surface of EVs from CRC patients, suggesting CD151(CD63) as a potential biomarker for early detection of CRC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
JunLiang Zhang, MengHan Chang, Xin Shi, Xing Zhou
Summary: In this study, a colorimetric sEVs detection strategy based on SMBs-capture probe and Exo-III assisted signal amplification was developed. The method exhibited a wide detection range and excellent capability in discriminating different types of sEVs. Additionally, the method also showed high accuracy and recovery rate when applied to clinical samples.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Lu Zheng, Hua Wang, Peng Zuo, Yueling Liu, Huiying Xu, Bang-Ce Ye
Summary: This study proposes a microfluidic chip for specific exosome isolation and sensitive quantification using a signal switch strategy. The platform enables efficient capture of exosomes and profiling of exosomal surface proteins, making it a potential tool for early cancer screening and diagnosis.
ANALYTICAL CHEMISTRY
(2022)
Article
Engineering, Biomedical
David Rufino-Ramos, Sevda Lule, Shadi Mahjoum, Stefano Ughetto, D. Cristopher Bragg, Luis Pereira de Almeida, Xandra O. Breakefield, Koen Breyne
Summary: The lack of techniques to trace brain cell behavior limits the ability to monitor their status. Extracellular vesicles (EVs), released by brain cells, might be able to report their status. However, identifying brain-derived EVs in complex biofluids is challenging. This study developed a sensitive platform to isolate pre-labelled brain-derived EVs from blood, allowing for the study of molecular fingerprints of brain cells.
Article
Microbiology
Sara B. York, Li Sun, Allaura S. Cone, Leanne C. Duke, Mujeeb R. Cheerathodi, David G. Meckes
Summary: Extracellular vesicles (EVs) are membrane-encapsulated structures released by cells that carry signaling factors, proteins, and microRNAs, playing an important role in the progression of neurological conditions and the spread of infectious diseases. Recent studies suggest that Zika virus may utilize EV pathways to package viral components and evade immune responses. This alternative means of Zika virus transmission highlights the potential role of EV biogenesis and trafficking proteins in modulating viral infection and virion morphogenesis.
Article
Chemistry, Analytical
Mohamed Sharafeldin, Shijun Yan, Cheng Jiang, George K. Tofaris, Jason J. Davis
Summary: We present a scalable, fast, and low-cost method for extracting cargo proteins from exosome subpopulations using an alternating (AC) magnetic field to facilitate the mixing of antibody-coated magnetic beads (MBs) with serum samples. By applying this method, we were able to achieve high capture efficiencies of >70% from <50 μL of neat serum in just 30 minutes. This approach, when combined with array-based assaying and a standard duplex electrochemical sandwich ELISA, enabled the detection of sub pg/mL levels of α-synuclein content with excellent precision and a sample-to-answer time of approximately 75 minutes. The high performance and semi-automation of this method hold promise for low-cost Parkinson's disease diagnostics and exosomal biomarker analyses.
ANALYTICAL CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Zhijing Yang, Da Liu, Hengzong Zhou, Boqiang Tao, Lu Chang, Huimin Liu, Haoming Luo, Dongxu Wang, Weiwei Liu
Summary: The study aimed to synthesize a new nanomaterial based on EVs containing chrysin and to carry AuNPs with EVs-chrysin to regulate let-7a-3p expression, inhibiting tumor growth in TSCC through cell apoptosis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Cell Biology
Ye Fan, Cedric Pionneau, Federico Cocozza, Pierre-Yves Boelle, Solenne Chardonnet, Stephanie Charrin, Clotilde Thery, Pascale Zimmermann, Eric Rubinstein
Summary: This study reveals that CD9, CD81, and CD63, commonly used as protein markers for extracellular vesicles (EVs), have little impact on the protein composition of EVs released by MCF7 breast cancer cells. Knocking out CD9 and CD81 only significantly decreases the levels of CD9P-1 and EWI-2 in EVs. This suggests a marginal role of CD9, CD81, and CD63 in determining the protein composition of EVs and highlights the regulation of CD9P-1 and EWI-2 expression level and/or trafficking by CD9 and CD81.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Article
Virology
Christos Dogrammatzis, Shadia Saleh, Clayton Deighan, Maria Kalamvoki
Summary: Extracellular vesicles play a crucial role in intercellular communication and can alter recipient cell functions despite their limited cargo capacity. This study identified distinct populations of EVs released during HSV-1 infection, with CD63(+) EVs being selectively induced and having a negative impact on the infection. The results suggest complex interactions between the virus and host through EVs, potentially affecting HSV-1 dissemination and persistence.
JOURNAL OF VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Izumi Yanatori, Fumio Kishi, Shinya Toyokuni
Summary: Ferritin is a protein for storing iron and is secreted from cells via extracellular vesicles or autophagy pathway. It functions as an iron-carrier protein in the extracellular environment and can suppress ferroptosis by secreting holo-ferritin.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Biochemical Research Methods
Zhenggang Hui, Ming Chang, Mingxian Hu
Summary: This study proposes a novel sEVs sensing system that integrates CD63 aptamer based identification and exonuclease III (Exo-III) assisted signal recycling. The method exhibits a wide detection range and low limit of detection for sEVs, and has been successfully applied for the study of sEVs expression in pneumonia patients and health volunteers.
ANALYTICAL BIOCHEMISTRY
(2022)
Review
Cell & Tissue Engineering
Wenzhao Cheng, Yiming Zeng, Dachun Wang
Summary: Pulmonary fibrosis is a chronic lung disease with limited treatment options. Lung transplantation is currently the only possible treatment, but it is limited by surgery complexity and donor scarcity. Stem cell therapy shows promise as an alternative treatment, although safety and efficacy issues need to be addressed.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Biochemistry & Molecular Biology
Xuefang Hao, Qian Li
Summary: Cell-based nanotherapy has immense potential in transforming the diagnosis and treatment of cardiovascular diseases (CVDs). Coating nanoparticles with cell membranes is an effective approach to enhance their biocompatibility, immune evasion, and specificity. Furthermore, extracellular vesicles (EVs) have emerged as promising tools for the diagnosis and therapy of CVDs due to their ability to transfer cargos to distant tissues. This review summarizes recent progress in cell-based nanotherapy for CVDs, highlighting different sources of EVs and biomimetic nanoplatforms derived from natural cells. It also discusses their potential applications in the diagnosis and targeted treatment of various CVDs, while addressing the challenges and future prospects.
MACROMOLECULAR BIOSCIENCE
(2023)
Review
Cell Biology
Phyllis I. Hanson, Anil Cashikar
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, VOL 28
(2012)
Article
Cell Biology
Byung Rho Lee, Yasuhiro Matsuo, Anil G. Cashikar, Tetsu Kamitani
JOURNAL OF CELL SCIENCE
(2013)
Article
Biochemistry & Molecular Biology
Snober S. Mir, David Fiedler, Anil G. Cashikar
MOLECULAR AND CELLULAR BIOLOGY
(2009)
Article
Biochemistry & Molecular Biology
Juhi Ojha, Gunasingh Masilamoni, David Dunlap, Ross A. Udoff, Anil G. Cashikar
MOLECULAR AND CELLULAR BIOLOGY
(2011)
Article
Genetics & Heredity
Esti Yeger-Lotem, Laura Riva, Linhui Julie Su, Aaron D. Gitler, Anil G. Cashikar, Oliver D. King, Pavan K. Auluck, Melissa L. Geddie, Julie S. Valastyan, David R. Karger, Susan Lindquist, Ernest Fraenkel
Article
Multidisciplinary Sciences
Shaiju K. Vareed, Vadiraja B. Bhat, Christopher Thompson, Vihas T. Vasu, Damian Fermin, Hyungwon Choi, Chad J. Creighton, Sitaram Gayatri, Ling Lan, Nagireddy Putluri, Gagan Singh Thangjam, Punit Kaur, Mohsen Shabahang, Judith G. Giri, Alexey I. Nesvizhskii, Alexander A. A. Asea, Anil G. Cashikar, Arundhati Rao, James McLoughlin, Arun Sreekumar
Article
Multidisciplinary Sciences
Juhi Ojha, Rajalakshmi V. Karmegam, J. Gunasingh Masilamoni, Alvin V. Terry, Anil G. Cashikar
Article
Biology
Anil G. Cashikar, Soomin Shim, Robyn Roth, Michael R. Maldazys, John E. Heuser, Phyllis I. Hanson
Article
Immunology
Man Ying Wong, Michael Lewis, James J. Doherty, Yang Shi, Anil G. Cashikar, Anna Amelianchik, Svitlana Tymchuk, Patrick M. Sullivan, Mingxing Qian, Douglas F. Covey, Gregory A. Petsko, David M. Holtzman, Steven M. Paul, Wenjie Luo
JOURNAL OF NEUROINFLAMMATION
(2020)
Article
Neurosciences
Yukitoshi Izumi, Anil G. Cashikar, Kathiresan Krishnan, Steven M. Paul, Douglas F. Covey, Steven J. Mennerick, Charles F. Zorumski
Summary: Inflammatory cells synthesize and release the oxysterol 25-hydroxycholesterol (25HC), which has antiviral and immunomodulatory properties. Activation of innate immunity by lipopolysaccharide (LPS) affects 25HC production in microglia and influences CA1 hippocampal synaptic plasticity and learning. 25HC serves as a key modulator of synaptic plasticity and memory under proinflammatory stimuli.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Clinical Neurology
Fabia Filipello, Shih-Feng You, Farzaneh S. Mirfakhar, Sidhartha Mahali, Bryan Bollman, Mariana Acquarone, Olena Korvatska, Jacob A. Marsh, Anirudh Sivaraman, Rita Martinez, Claudia Cantoni, Luca De Feo, Laura Ghezzi, Miguel A. Minaya, Arun Renganathan, Anil G. Cashikar, Jun-Ichi Satoh, Wandy Beatty, Abhirami K. Iyer, Marina Cella, Wendy H. Raskind, Laura Piccio, Celeste M. Karch
Summary: This study found that iMGLs derived from NHD patients showed lysosomal dysfunction, downregulation of cholesterol genes, and reduced lipid droplets, which were also observed in post-mortem brain tissues from NHD patients. The study also found that enhancing lysosomal biogenesis can restore these defects. This finding provides new insights into the mechanisms underlying NHD and other neurodegenerative diseases.
ACTA NEUROPATHOLOGICA
(2023)
Meeting Abstract
Biochemistry & Molecular Biology
Chaya Gopalan, Zach Colley, Paige Niepoetter, Christopher Waller, Anil Cashikar
Article
Oncology
Ling Xia, Lahcen Jaafar, Anil Cashikar, Hernan Flores-Rozas
Article
Multidisciplinary Sciences
Antony A. Cooper, Aaron D. Gitler, Anil Cashikar, Cole M. Haynes, Kathryn J. Hill, Bhupinder Bhullar, Kangning Liu, Kexiang Xu, Katherine E. Strathearn, Fang Liu, Songsong Cao, Kim A. Caldwell, Guy A. Caldwell, Gerald Marsischky, Richard D. Kolodner, Joshua LaBaer, Jean-Christophe Rochet, Nancy M. Bonini, Susan Lindquist