期刊
PLOS ONE
卷 14, 期 6, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0218318
关键词
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资金
- Chan Zuckerberg Biohub
- Chan Zuckerberg Initiative
- Howard Hughes Medical Institute
- National Institutes of Health
- Doris Duke Charitable Foundation
- Rachleff Foundation
- NINDS [K08NS096117]
- University of California, San Francisco Center for Next-Gen Precision Diagnostics - Sandler Foundation
- William K. Bowes, Jr. Foundation
- NHLBI [K23HL138461-01A1]
- Nina Ireland Foundation
- Marcus Program in Precision Medicine
- UC Berkeley UC San Francisco Joint Program in Bioengineering
Febrile illness is a major burden in African children, and non-malarial causes of fever are uncertain. In this retrospective exploratory study, we used metagenomic next-generation sequencing (mNGS) to evaluate serum, nasopharyngeal, and stool specimens from 94 children (aged 2-54 months) with febrile illness admitted to Tororo District Hospital, Uganda. The most common microbes identified were Plasmodium falciparum (51.1% of samples) and parvovirus B19 (4.4%) from serum; human rhinoviruses A and C (40%), respiratory syncytial virus (10%), and human herpesvirus 5 (10%) from nasopharyngeal swabs; and rotavirus A (50% of those with diarrhea) from stool. We also report the near complete genome of a highly divergent orthobunyavirus, tentatively named Nyangole virus, identified from the serum of a child diagnosed with malaria and pneumonia, a Bwamba orthobunyavirus in the nasopharynx of a child with rash and sepsis, and the genomes of two novel human rhinovirus C species. In this retrospective exploratory study, mNGS identified multiple potential pathogens, including 3 new viral species, associated with fever in Ugandan children.
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