Effects of different carotenoid supplementation sources with or without sodium taurocholate on growth, feed utilization, carotenoid content and antioxidant status in fry of the European seabass, Dicentrarchus labrax

A total of 210 European seabass fry with an average body weight of 0.51 +/- 0.03g were divided into seven experimental treatments (three replicates per treatment). The seven experimental diets (45.64% CP) were formulated to contain synthetic astaxanthin (SA; 0.1gkg(-1)), marigold flower meal (MFM; 0.2gkg(-1)) or crab waste meal (CM; 10gkg(-1)), with or without sodium taurocholate (ST; 0.4gkg(-1)); the control diet contained no supplementation. The experiment was conducted for 8weeks. The results show that seabass fry fed the diets containing different sources of carotenoids, especially with ST, demonstrated higher growth efficiency, survival (%), feed efficiency, protein and ether extract contents of fish body. The best results were found with Diet(MFM+ST). Whole-body carotenoid levels increased significantly with all supplementation treatments; the highest level was observed with SA, followed by fish fed Diet(MFM) and Diet(CM), especially with ST co-supplementation. Moreover, thiobarbituric acid-reactive substances were significantly reduced in fish fed the diets containing different sources of carotenoids compared to the control diet. The total antioxidant status significantly increased in fish fed Diet(SA) and Diet(CM) alone or with ST compared to the control group. In conclusion, these results reveal that Diet(MFM+ST) is the most suitable treatment for improving growth, feed efficiency, and the protein and ether extract contents of seabass fry compared to other sources of carotenoids. The best oxidant/antioxidant balance and carotenoid content were achieved using SA, CM and MFM with ST supplementation.