Article
Multidisciplinary Sciences
Jung Hyun Bae, Myung Jin Yang, Seung-Hwan Jeong, JungMo Kim, Seon Pyo Hong, Jin Woo Kim, Yoo Hyung Kim, Gou Young Koh
Summary: In this study, the researchers discovered that Merlin, encoded by the neurofibromatosis type 2 (NF2) gene, suppresses VEGFR2 internalization and controls the induction of tip endothelial cells (EC). Depletion of Nf2 in endothelial cells enhances VEGFR2 internalization and leads to excessive filopodia formation in tip EC induction. Merlin binds to the VEGFR2-VE-cadherin complex and reduces downstream signaling, thus affecting the induction of tip ECs.
Article
Oncology
Pedro Torres-Ayuso, Elvira An, Katherine M. Nyswaner, Ryan C. Bensen, Daniel A. Ritt, Suzanne Specht, Sudipto Das, Thorkell Andresson, Raul E. Cachau, Roger J. Liang, Amy L. Ries, Christina M. Robinson, Simone Difilippantonio, Brad Gouker, Laura Bassel, Baktiar O. Karim, Chad J. Miller, Benjamin E. Turk, Deborah K. Morrison, John Brognard
Summary: Research identified TNIK as a potential therapeutic target in LSCC, with genetic depletion or pharmacologic inhibition showing efficacy in reducing the growth of LSCC cells. In preclinical models, TNIK inhibition displayed antitumor activity and increased apoptosis in LSCC patient-derived xenografts.
Article
Multidisciplinary Sciences
Anne E. Roehrig, Kristina Klupsch, Juan A. Oses-Prieto, Selim Chaib, Stephen Henderson, Warren Emmett, Lucy C. Young, Silvia Surinova, Andreas Blees, Anett Pfeiffer, Maha Tijani, Fabian Brunk, Nicole Hartig, Marta Munoz-Alegre, Alexander Hergovich, Barbara H. Jennings, Alma L. Burlingame, Pablo Rodriguez-Viciana
Summary: The study investigates the roles of the PAF complex and Merlin tumor suppressor protein in RNA processing, highlighting their interaction for growth suppression. Additionally, the involvement of FAT cadherins in regulating Merlin-PAFC interaction is identified as a significant finding from the research.
Article
Cell Biology
Qi Tian, Huan Gao, Yan Zhou, Lizhe Zhu, Jiao Yang, Bo Wang, Peijun Liu, Jin Yang
Summary: This study elucidates the role and molecular mechanism of RICH1 in regulating stemness of breast cancer cells. RICH1 activates the kinase cascade of the Hippo signaling pathway by displacing Amot-p80 from the complex with Merlin, thereby promoting stemness and inhibiting migration and invasion of breast cancer cells.
CELL DEATH & DISEASE
(2022)
Article
Pathology
David B. Chapel, Jason L. Hornick, Julianne Barlow, Raphael Bueno, Lynette M. Sholl
Summary: BAP1 and MTAP immunostains are important for the diagnosis of mesothelioma, but additional markers are needed to increase sensitivity. Immunopanels and panel NGS testing are effective in improving the diagnostic accuracy of mesothelioma.
Article
Biochemistry & Molecular Biology
Ara Yoo, Soojin Lee
Summary: NEGR1 interacts with IL-6R and negatively regulates IL-6 trans-signaling by attenuating the phosphorylation of STAT3 by sIL-6R. This may contribute to a molecular link underlying obesity, inflammation, and the depression cycle.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Plant Sciences
Lichun Cao, Jiaqi Tian, Yilin Liu, Xiaofei Chen, Siqi Li, Staffan Persson, Dan Lu, Mingjiao Chen, Zhijing Luo, Dabing Zhang, Zheng Yuan
Summary: OsJAZ6 interacts with OsJAZ1 in rice spikelet development, with OsJAZ6 being more sensitive to JA-mediated degradation and orchestrating the output of JA signaling. The study discusses a possible mechanism of how OsJAZ repressors interact and function synergistically in specifying JA signaling output through degradation titration.
Article
Biochemistry & Molecular Biology
Milian Yang, Xiao Han, Jiajia Yang, Yanjuan Jiang, Yanru Hu
Summary: Seed germination and postgerminative growth require precise coordination of multiple intrinsic and environmental signals. Core circadian clock proteins PRR5 and PRR7 physically associate with the crucial ABA signaling transcription factor ABI5, positively modulating ABA signaling during seed germination.
Article
Biochemistry & Molecular Biology
Richa Mishra, Aurosikha Das, Soumendra Rana
Summary: Resveratrol, a promiscuous phytoalexin found in fruits and nuts, exhibits health benefits through antioxidant and anti-inflammatory activities. It directly binds to COX-1, COX-2 enzymes, and (h)C5a, modulating inflammatory pathways and showing anti-inflammatory effects.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Article
Oncology
Eleonora Sementino, Yuwaraj Kadariya, Mitchell Cheung, Craig W. Menges, Yinfei Tan, Anna-Mariya Kukuyan, Ujjawal Shrestha, Sofiia Karchugina, Kathy Q. Cai, Suraj Peri, James S. Duncan, Jonathan Chernoff, Joseph R. Testa
Summary: In this study, the researchers found that loss of Pak2 reduces the incidence and delays the onset of malignant mesothelioma, and affects cell viability and migration. They also observed downregulation of Hedgehog and Wnt pathway gene expression and kinase changes indicative of EMT in Pak2-deficient malignant mesothelioma cells.
MOLECULAR CANCER RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Adrienn Skopal, Tamas Keki, Peter A. Toth, Balazs Csoka, Balazs Koscso, Zoltan H. Nemeth, Luca Antonioli, Andreas Ivessa, Francisco Ciruela, Laszlo Virag, Gyorgy Hasko, Endre Kokai
Summary: In this study, the researchers identified CtsD as an interacting partner of A(2A)R in macrophages and found that this interaction plays a crucial role in adenosine-mediated macrophage regulation in inflammatory processes.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Cell Biology
Simon Bennet Sonnenberg, Jonah Rauer, Christoph Gohr, Nataliya Gorinski, Sophie Kristin Schade, Dalia Abdel Galil, Vladimir Naumenko, Andre Zeug, Stephan C. Bischoff, Evgeni Ponimaskin, Daria Guseva
Summary: The study uncovered a signaling pathway involving L1CAM and 5-HT4 receptor that facilitates dendritic spine maturation and affects memory formation.
JOURNAL OF CELL SCIENCE
(2021)
Article
Gastroenterology & Hepatology
Lu Tian, Luqing Zhao, Karen Man-Fong Sze, Charles Shing Kam, Vanessa Sheung-In Ming, Xia Wang, Vanilla Xin Zhang, Daniel Wai-Hung Ho, Tan-To Cheung, Lo-Kong Chan, Irene Oi-Lin Ng
Summary: RalA is significantly up-regulated in HCC patients and associated with aggressive tumor behavior and poor prognosis. Its up-regulation is driven by copy number gain, while RalGAPA2 knockdown increases RalA activity. Tumors with down-regulated RalGAPA2 and up-regulated RalA display more aggressive behavior and poorer survival, with Ral inhibition showing potential as a therapeutic approach.
Article
Biochemistry & Molecular Biology
Kunrong He, Jiancan Du, Xiao Han, Huiqiong Li, Mengyi Kui, Juping Zhang, Zhichong Huang, Qiantang Fu, Yanjuan Jiang, Yanru Hu
Summary: PHR1 works with JAZ repressors and MYC2 to regulate jasmonate signaling in response to phosphate deficiency. The study confirmed that jasmonate signaling is enhanced under low phosphate conditions, and the COI1-mediated pathway is crucial for this process. PHR1 interacts with JAZ repressors and MYC2 to stimulate jasmonate-responsive gene expression and enhance jasmonate responses.
Article
Oncology
Zhendan Zhao, Zhiling Wang, Pengling Wang, Shujie Liu, Yingwei Li, Xingsheng Yang
Summary: The study identified EPDR1 as a tumor-suppressor gene negatively regulated by miR-429 in EOC development, inhibiting proliferation, migration, and invasion of cancer cells through the PI3K/AKT pathway. The miR-429/EPDR1 axis may offer novel therapeutic targets for personalized treatment of EOC patients in the future.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemical Research Methods
Shuting Han, Yan Cui, Dario L. Helbing
BIOTECHNOLOGY JOURNAL
(2020)
Article
Biochemical Research Methods
Yan Cui
ANALYTICAL BIOCHEMISTRY
(2020)
Article
Cell Biology
Alexander Schulz, Yuichi Sekine, Motunrayo J. Oyeyemi, Alexander J. Abrams, Manasa Basayaraju, Sung Min Han, Marco Groth, Helen Morrison, Stephen M. Strittmatter, Marc Hammartund
JOURNAL OF CELL BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Said C. Farschtschi, Tina Mainka, Markus Glatzel, Anna-Lena Hannekum, Michael Hauck, Mathias Gelderblom, Christian Hagel, Reinhard E. Friedrich, Martin U. Schuhmann, Alexander Schulz, Helen Morrison, Hildegard Kehrer-Sawatzki, Jan Luhmann, Christian Gerloff, Martin Bendszus, Philipp Baeumer, Victor-Felix Mautner
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Editorial Material
Clinical Neurology
Dario-Lucas Helbing, Michael Brodhun, Oliver Tiedge, Helen Morrison, Steffen K. Rosahl
MULTIPLE SCLEROSIS AND RELATED DISORDERS
(2020)
Article
Oncology
Junzhi Ma, Janina Klemm, Monserrat Gerardo-Ramirez, Lucien Frappart, Darko Castven, Diana Becker, Ansgar Zoch, Romain Parent, Birke Bartosch, Kerstin Minnich, Marco Giovannini, Sven Danckwardt, Nils Hartmann, Helen Morrison, Peter Herrlich, Jens U. Marquardt, Monika Hartmann
INTERNATIONAL JOURNAL OF CANCER
(2020)
Review
Cell Biology
Leopold Boehm, Dario-Lucas Helbing, Nova Oraha, Helen Morrison
MECHANISMS OF AGEING AND DEVELOPMENT
(2020)
Article
Biochemistry & Molecular Biology
Yan Cui, Lin Ma, Stephan Schacke, Jiani C. Yin, Yi-Ping Hsueh, Hongchuan Jin, Helen Morrison
HUMAN MOLECULAR GENETICS
(2020)
Article
Chemistry, Medicinal
Heping Zhu, Shilong Ying, Bingluo Zhou, Xiao Liang, Quan He, Ping Song, Xinyang Hu, Keqiang Shi, Mingteng Xiong, Hongchuan Jin, Yuanjiang Pan
Summary: A series of novel 2-aryl-3-sulfonamido-pyridines (HoAns) compounds were designed, synthesized, and evaluated for their antiproliferative activities, with HoAn32 demonstrating the most potent activity and microtubule-targeting effect in cancer cell lines. Mechanism studies showed that HoAn32 bound to the colchicine site of b-tubulin, resulting in multiple anti-cancer effects.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Long-Sheng Chang, Janet L. Oblinger, Abbi E. Smith, Marc Ferrer, Steven P. Angus, Eric Hawley, Alejandra M. Petrilli, Roberta L. Beauchamp, Lars Bjorn Riecken, Serkan Erdin, Ming Poi, Jie Huang, Waylan K. Bessler, Xiaohu Zhang, Rajarshi Guha, Craig Thomas, Sarah S. Burns, Thomas S. K. Gilbert, Li Jiang, Xiaohong Li, Qingbo Lu, Jin Yuan, Yongzheng He, Shelley A. H. Dixon, Andrea Masters, David R. Jones, Charles W. Yates, Stephen J. Haggarty, Salvatore La Rosa, D. Bradley Welling, Anat O. Stemmer-Rachamimov, Scott R. Plotkin, James F. Gusella, Justin Guinney, Helen Morrison, Vijaya Ramesh, Cristina Fernandez-Valle, Gary L. Johnson, Jaishri O. Blakeley, D. Wade Clapp
Summary: NF2 is a genetic syndrome caused by NF2 gene mutations, lacking FDA approved therapies, but brigatinib has shown potential efficacy for NF2 related tumors, acting through inhibition of multiple tyrosine kinases with a mechanism different from traditional drugs.
Article
Biochemistry & Molecular Biology
Wen Ting Cai, Wha Young Kim, Myung Ji Kwak, Haeun Rim, Seung Eun Lee, Lars Bjorn Riecken, Helen Morrison, Jeong-Hoon Kim
Summary: Repeated injections of psychomotor stimulants can induce behavioral sensitization and lead to addictive behaviors. This study found that a specific mutant can inhibit the development of sensitization, providing important clues for understanding the role of dendritic spines in psychomotor stimulant addiction.
JOURNAL OF NEUROCHEMISTRY
(2022)
Article
Neurosciences
Stephan Schacke, Joanna Kirkpatrick, Amy Stocksdale, Reinhard Bauer, Christian Hagel, Lars Bjoern Riecken, Helen Morrison
Summary: Astrocytes play a crucial role in brain function and behavior through their involvement in synaptic signaling and ion homeostasis. Ezrin, an essential protein in astrocytes, has been found to contribute to synaptic transmission and astrocyte activation. Deletion of Ezrin in mice resulted in behavioral changes and showed a protective effect in an experimental ischemic stroke. These findings suggest the importance of Ezrin in astrocyte functions and its potential therapeutic implications.
Article
Cell & Tissue Engineering
Na Li, Ying Cai, Min Zou, Jian Zhou, Ling Zhang, Liquan Zhou, Wenpei Xiang, Yan Cui, Huaibiao Li
Summary: This study analyzed the 3' untranslated region (3' UTR) lengths in human and mouse pre-implantation embryos using single-cell RNA sequencing data. The results showed distinct patterns of 3' UTR changing between the two species, but RNA metabolism pathways were involved in both. The abundance of CFIm components NUDT21 and CPSF6 in the nucleus likely determines the 3' UTR lengths. Depletion of these components led to early embryo development arrest and 3' UTR shortening. Overall, these findings highlight the essential role of APA in the development of mammalian early embryos.
Article
Polymer Science
Dario Lucas Helbing, Leopold Boehm, Nova Oraha, Leonie Karoline Stabenow, Yan Cui
Summary: Protein quantification is often unreliable for tissue samples, leading to uneven loading in subsequent experiments. We propose a Ponceau S staining-based dot-blot assay as a more reliable and simpler alternative to the widely used BCA assay. This method is compatible with various biological samples and provides accurate protein quantification.
Article
Biochemistry & Molecular Biology
Heping Zhu, Shilong Ying, Bingluo Zhou, Xinyang Hu, Xiao Liang, Wangyu Li, Dungai Wang, Hongchuan Jin, Yuanjiang Pan
Summary: A novel compound ID-11 was designed and synthesized to effectively inhibit the proliferation of CRC cells while showing minimal impact on normal colon epithelial cells. Mechanism studies suggest that ID-11 may inhibit CRC cell proliferation through multiple pathways, and it exhibits favorable pharmacokinetic properties.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2021)