Article
Virology
Sebastian O. Wendel, Avanelle Stoltz, Xuan Xu, Jazmine A. Snow, Nicholas Wallace
Summary: A subset of human papillomaviruses (HPVs), particularly HPV16, play a crucial role in the development of cervical cancer. The HPV16 E7 oncogene destabilizes RB and facilitates viral replication, while HPV16 E6 degrades p53 to mitigate replication stress. This study shows that cervical cancer cells activate the translesion synthesis (TLS) pathway to prevent replication fork collapse caused by stress. Increased expression of TLS genes is more common in cervical cancer than in other cancer types, and is associated with improved survival rates.
Article
Oncology
Lucia Oton-Gonzalez, John Charles Rotondo, Carmen Lanzillotti, Elisa Mazzoni, Ilaria Bononi, Maria Rosa Iaquinta, Luca Cerritelli, Nicola Malagutti, Andrea Ciorba, Chiara Bianchini, Stefano Pelucchi, Mauro Tognon, Fernanda Martini
Summary: Classical markers alone may not be enough to classify HPV-positive HNSCC patients, but when combined with serological markers, they are strong prognostic indicators in OPSCC patients. Detection of HPV16 E7 oncoprotein in serum at diagnosis is correlated with disease recurrence and patient overall survival, providing a useful tool for stratifying and monitoring HPV-positive HNSCC patients for worse prognosis.
Article
Oncology
Jiang Zhu, Lingjing Chen, Qiaoli Zheng, Rui Han, Xianzhen Chen, Qiang Zhou, Hao Cheng
Summary: This study aimed to establish a reliable protocol for immunohistochemistry stains to detect HPV16 E7 protein and found a positive correlation between HPV16 E7 protein expression and malignancy grade.
Review
Virology
Mwiza Gideon Singini, Elvira Singh, Debbie Bradshaw, Thendo Ramaliba, Wenlong Carl Chen, Melitah Motlhale, Abram Bunya Kamiza, Chantal Babb de Villiers, Mazvita Muchengeti, Christopher G. Mathew, Robert Newton, Noemi Bender, Tim Waterboer, Freddy Sitas
Summary: This article reviewed the literature on the importance of selected anti-high-risk human papillomavirus (HR-HPV) antibodies as potential serological markers for early detection of individuals at high risk of cervical cancer. The study found that HR-HPV16/18 E6 and E7 serological markers showed high specificity, but sensitivity was suboptimal for the detection of cervical cancer.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Review
Oncology
Laura Downham, Iman Jaafar, Mary Luz Rol, Victoria Nyawira Nyaga, Joan Valls, Armando Baena, Li Zhang, Marc J. Gunter, Marc Arbyn, Maribel Almonte
Summary: This study evaluated the accuracy of HPV-E6/E7 oncoprotein tests in detecting cervical precancer and cancer through a systematic review and meta-analysis. The results showed that oncoprotein tests have high specificity and can be used for triaging HPV-positive women. However, further follow-up is needed for oncoprotein-negative women.
BRITISH JOURNAL OF CANCER
(2023)
Article
Virology
Ting Li, Zhiping Yang, Chunlin Zhang, Sutong Wang, Bing Mei
Summary: In central China, HPV-16 E6 and E7 genes exhibit multiple variations, and a nucleotide variation (A647G, N29S) is significantly associated with a higher risk of cervical cancer. Variations in the E6 gene affect multiple B-cell epitopes, while variations in the E7 gene have minimal impact on B-cell epitopes. These variation data have important implications for HR-HPV prevention and vaccine development.
Article
Biochemistry & Molecular Biology
Sivasangkary Gandhi, Nurshamimi Nor Rashid, Muhammad Fazril Mohamad Razif, Shatrah Othman
Summary: This study found that transfection with HPV 16/18 E7 leads to the loss of p130 and deregulation of the cell cycle, resulting in impaired cellular differentiation and increased cytoplasmic p130 levels in HaCaT cells. The findings suggest that disruption of the nuclear localization of p130 by HPV 16/18 E7 contributes to cell cycle dysregulation and impaired cellular differentiation ultimately leading to cellular transformation.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Oncology
Xinmei Wang, Guangnan Shuai, Junhui Xu, Meihua Liu, Jianguo Zhao, Na Zhang, Wenwen Zhang, Pengpeng Qu
Summary: This study investigated the application of quantitative HPV16 E7 oncoprotein detection in cervical cancer screening. The results showed a positive correlation between the level of HPV16 E7 oncoprotein and the degree of cervical lesions, with a cut-off value of 8.68 ng/ml for identifying HSIL+. This method demonstrated good diagnostic value and predictive ability for disease progression.
FRONTIERS IN ONCOLOGY
(2023)
Article
Oncology
Neha Jaiswal, Deeptashree Nandi, Pradeep Singh Cheema, Alo Nag
Summary: The study identifies Cdh1 as a novel interacting partner and degradation target of the HPV E7 oncoprotein, and demonstrates its importance in cervical carcinogenesis. The findings contribute to our understanding of E7-induced carcinogenesis and provide a potential therapeutic target for cervical carcinoma management.
Article
Microbiology
Ramon Antano-Arias, Oscar Del Moral-Hernandez, Julio Ortiz-Ortiz, Luz Del Carmen Alarcon-Romero, Jorge Adan Navor-Hernandez, Marco Antonio Leyva-Vazquez, Marco Antonio Jimenez-Lopez, Jorge Organista-Nava, Berenice Illades-Aguiar
Summary: This study identified specific E7 variants associated with the highest risk of cervical cancer, with E6/E7 bicistron analysis revealing even greater risk potential for specific variants. These findings underscore the importance of analyzing combinations of E6/E7 variants in HPV 16 infection for predicting cervical cancer development.
Article
Biochemistry & Molecular Biology
Diego Carrillo-Beltran, Julio C. Osorio, Rances Blanco, Carolina Oliva, Enrique Boccardo, Francisco Aguayo
Summary: Although epidemiological studies suggest that tobacco smoking and HR-HPV infection are mutually exclusive risk factors for developing HNC, some individuals are both HPV-positive and smokers. Both factors increase oxidative stress and DNA damage. This study found that oral cells expressing HPV16 E6/E7 oncoproteins exposed to cigarette smoke condensate showed synergistic increases in SOD2 levels and DNA damage, independent of Akt1 and ATM. These findings suggest that the interaction between HPV and cigarette smoke in HNC promotes SOD2 alterations, leading to increased DNA damage and the development of a distinct clinical entity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Lina Zhang, Xinyi Shi, Qing Zhang, Zhilei Mao, Xiaoyu Shi, Jun Zhou, Aili Jian, Renying Zhu, Shisong Jiang, Wenshu Lu
Summary: High-risk human papillomavirus (HPV) infection is the main cause of cervical cancer, and the specific T cell response to HPV16 E7 in peripheral blood mononuclear cells is related to HPV16 clearance and protection against malignant development.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Virology
Kerry Dust, Michael Carpenter, Julie Chih-yu Chen, Chris Grant, Stuart McCorrister, Garret R. Westmacott, Alberto Severini
Summary: This study investigates the effects of HPV16 viral proteins E6 and E7 on the host proteome in human neonatal keratinocytes. The results show that the expression of E6 and E7 leads to dysregulation in pathways related to interferon response, inflammation, and DNA damage repair. The study also identifies potential biomarkers associated with HPV oncoproteins in primary keratinocytes.
Article
Oncology
Fariba Dorostkar, Arash Arashkia, Farzin Roohvand, Zabihollah Shoja, Mohsen Navari, Maryam Mashhadi Abolghasem Shirazi, Zahra Shahosseini, Mohammad Farahmand, Mohammad Sadegh Shams Nosrati, Somayeh Jalilvand
Summary: This study aimed to develop a therapeutic vaccine based on mutated E7 protein for cervical cancer treatment. The results showed that E7GRG + 2'-3 'cGAMP + CpG-C induced strong cell-mediated and humoral immune responses, leading to inhibition of tumor growth in mice model.
INFECTIOUS AGENTS AND CANCER
(2021)
Article
Oncology
Nima Hemmat, Mohammad Amin Doustvandi, Zahra Asadzadeh, Ahad Mokhtarzadeh, Behzad Baradaran, Hossein Bannazadeh Baghi
Summary: The study demonstrates that silencing the E5 protein in cervical cancer cells can slow down cell proliferation and increase apoptosis, which may help inhibit the progression of cervical cancer.
Article
Cell Biology
Haiwei Jia, Xiaojuan Zhang, Wenjun Wang, Yuanyuan Bai, Youguo Ling, Cheng Cao, Runlin Z. Ma, Hui Zhong, Xue Wang, Quanbin Xu
Article
Immunology
Kai Guan, Congwen Wei, Zirui Zheng, Ting Song, Feixiang Wu, Yanhong Zhang, Ye Cao, Shengli Ma, Wei Chen, Quanbin Xu, Weiwei Xia, Jun Gu, Xiang He, Hui Zhong
JOURNAL OF IMMUNOLOGY
(2015)
Review
Pharmacology & Pharmacy
Quanbin Xu, Dane D. Jensen, Hua Peng, Yumei Feng
PHARMACOLOGY & THERAPEUTICS
(2016)
Article
Biochemistry & Molecular Biology
Guang-Fei Wang, Qincai Dong, Yuanyuan Bai, Jing Yuan, Quanbin Xu, Cheng Cao, Xuan Liu
FREE RADICAL BIOLOGY AND MEDICINE
(2017)
Article
Biochemistry & Molecular Biology
Gilson J. Sanchez, Phillip A. Richmond, Eric N. Bunker, Samuel S. Karman, Joseph Azofeifa, Aaron T. Garnett, Quanbin Xu, Graycen E. Wheeler, Cathryn M. Toomey, Qinghong Zhang, Robin D. Dowell, Xuedong Liu
NUCLEIC ACIDS RESEARCH
(2018)
Article
Biochemistry & Molecular Biology
Jiawei Jin, Xiaoqian Shi, Yongchao Li, Qianyu Zhang, Yu Guo, Chaokun Li, Pingping Tan, Qiuhong Fang, Yingmin Ma, Runlin Z. Ma
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Youguo Ling, Xiaojuan Zhang, Yuanyuan Bai, Ping Li, Congwen Wei, Ting Song, Zirui Zheng, Kai Guan, Yanhong Zhang, Buchang Zhang, Xuedong Liu, Runlin Z. Ma, Cheng Cao, Hui Zhong, Quanbin Xu
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2014)
Article
Cell Biology
Xiaojuan Zhang, Youguo Ling, Wenjun Wang, Yanhong Zhang, Qingjun Ma, Pingping Tan, Ting Song, Congwen Wei, Ping Li, Xuedong Liu, Runlin Z. Ma, Hui Zhong, Cheng Cao, Quanbin Xu
Article
Virology
Changzhi Xu, Xiang He, Zirui Zheng, Zhe Zhang, Congwen Wei, Kai Guan, Lihua Hou, Buchang Zhang, Lin Zhu, Yuan Cao, Yanhong Zhang, Ye Cao, Shengli Ma, Penghao Wang, Pingping Zhang, Quanbin Xu, Youguo Ling, Xiao Yang, Hui Zhong
JOURNAL OF VIROLOGY
(2014)
Article
Biochemistry & Molecular Biology
Kai Guan, Zirui Zheng, Ting Song, Xiang He, Changzhi Xu, Yanhong Zhang, Shengli Ma, Ying Wang, Quanbin Xu, Ye Cao, Jia Li, Xiaoli Yang, Xiaoxing Ge, Congwen Wei, Hui Zhong
MOLECULAR AND CELLULAR BIOLOGY
(2013)
Article
Multidisciplinary Sciences
Congwen Wei, Yuan Cao, Xiaoli Yang, Zirui Zheng, Kai Guan, Qiang Wang, Yanhong Tai, Yanhong Zhang, Shengli Ma, Ye Cao, Xiaoxing Ge, Changzhi Xu, Jia Li, Hui Yan, Youguo Ling, Ting Song, Lin Zhu, Buchang Zhang, Quanbin Xu, Chengjin Hu, Xiu-wu Bian, Xiang He, Hui Zhong
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2014)
Article
Biotechnology & Applied Microbiology
Chaokun Li, Longxin Chen, Xuefeng Liu, Xiaoqian Shi, Yu Guo, Rui Huang, Fangyuan Nie, Changming Zheng, Chenglin Zhang, Runlin Z. Ma
Article
Immunology
Chaokun Li, Rui Huang, Fangyuan Nie, Jiujie Li, Wen Zhu, Xiaoqian Shi, Yu Guo, Yan Chen, Shiyu Wang, Limeng Zhang, Longxin Chen, Runting Li, Xuefeng Liu, Changming Zheng, Chenglin Zhang, Runlin Z. Ma
FRONTIERS IN IMMUNOLOGY
(2020)