Article
Microbiology
Katherina Garcia Vanegas, Jakob Kraemmer Haar Rendsvig, Zofia Dorota Jarczynska, Marcio Vinicius de Carvalho Barros Cortes, Abel Peter van Esch, Marti Morera-Gomez, Fabiano Jares Contesini, Uffe Hasbro Mortensen
Summary: The introduction of CRISPR technologies in filamentous fungi has greatly impacted strain engineering. However, concerns over intellectual property ownership have hindered its commercial applications. The Mad7 enzyme shows promise as a solution to the complex IP constraints, and we have developed a versatile Mad7-CRISPR vector-set that can efficiently be used for genetic engineering in four different Aspergillus species. Our system enables marker-free gene editing and is functional in both NHEJ-proficient and deficient strains, allowing for gene deletions and integrations using different types of DNA repair.
Article
Chemistry, Multidisciplinary
Caiyan Zhao, Yaya Cheng, Pei Huang, Changrong Wang, Weipeng Wang, Mengjiao Wang, Wenbo Shan, Hongzhang Deng
Summary: X-CC9 system enables selective activation of therapeutic agents at tumor sites through X-ray guidance and remote control, leading to M2-to-M1 repolarization within the tumor microenvironment. This strategy significantly improves antitumor efficacy and induces robust immune responses.
ADVANCED MATERIALS
(2023)
Review
Biotechnology & Applied Microbiology
Alaa Hassanien, Imen Saadaoui, Kira Schipper, Sara Al-Marri, Tasneem Dalgamouni, Mustapha Aouida, Suhur Saeed, Hareb M. M. Al-Jabri
Summary: In recent years, there has been growing interest in reusing produced water due to increased demand and regional variability of available water resources, as well as the need for sustainable water supply planning. Bioremediation using microalgae has emerged as a promising method for treating produced water, removing major contaminants and even heavy metals and hydrocarbons. Genetic modification of microalgae has been considered as a novel approach to enhance their capabilities and phytoremediation of wastewater. This article provides a comprehensive overview of using genetically engineered microalgae for wastewater bioremediation, discussing various methods and the opportunities and challenges associated with this approach.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Agricultural Engineering
Tse-Min Lee, Jia-Yi Lin, Tsung-Han Tsai, Ru-Yin Yang, I-Son Ng
Summary: Carbon dioxide is the main greenhouse gas and a critical issue for global warming and climate change. Microalgae, which are inconspicuous microorganisms, account for 40% of carbon fixation in photosynthetic plants and have higher photosynthetic efficiency. They convert carbon into various useful compounds. Genetic engineering and metabolic engineering are used to increase the growth rate and biomass of microalgae to achieve carbon neutrality. CRISPR/Cas9 is an efficient tool to enhance productivity in microalgae by regulating multiple genes simultaneously. This review summarizes the technologies, bioinformatics tools, and systematic engineering approaches for carbon neutrality and circular economy, bringing us closer to a decarbonized society.
BIORESOURCE TECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Yingxiao Zhang, Brian Iaffaldano, Yiping Qi
Summary: CRISPR-derived biotechnologies have revolutionized genetic engineering in plants, but still face challenges such as editing efficiency and off-target mutagenesis.
PLANT COMMUNICATIONS
(2021)
Article
Engineering, Environmental
Jinjin Chen, Yilan Liu, Radhakrishnan Mahadevan
Summary: Synthetic biology provides a promising solution for improving Acidithiobacillus strains in biomining. The researchers established a CRISPR-dCas9 system for gene knockdown and an all-in-one CRISPR-Cas9 system for efficient genome editing in A. ferridurans JAGS. The engineered strain showed reduced production of toxic substances during biomining experiments.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Review
Cell Biology
Rahul Gupta, Arijit Ghosh, Rudra Chakravarti, Rajveer Singh, Velayutham Ravichandiran, Snehasikta Swarnakar, Dipanjan Ghosh
Summary: The discovery of CRISPR-Cas systems has revolutionized genetic alterations, with Cas13d showing high efficiency and specificity in cleaving targeted RNA in mammalian systems, making it a potential tool for RNA engineering and editing.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Rachma Wikandari, Manikharda, Susanne Baldermann, Andriati Ningrum, Mohammad J. Taherzadeh
Summary: Modern biotechnology, including cell culture, marker assisted selection, and genetic engineering, accelerates food production by reducing land usage, increasing genetic gain, introducing desired traits, and enhancing resistance. Challenges and future prospects need to be further explored.
Article
Biotechnology & Applied Microbiology
Jianfei Wang, Shijie Liu, Jiaqi Huang, Ran Cui, Yulei Xu, Zhilin Song
Summary: Polyhydroxyalkanoates (PHAs) are biopolymers with excellent biocompatibility and biodegradability, making them promising for biomedical applications. However, high production costs hinder commercialization, which can be addressed by using carbon-rich wastes as substrates. Metabolic engineering strategies, including genetic modifications and CRISPR/Cas9-based systems, have been utilized to improve PHA production efficiency by enhancing substrate assimilation and PHA synthesis.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2023)
Article
Business
Pierre Berthon, Christine Pitt, Andrew Park, Leyland Pitt
Summary: Many industries and their offerings have suffered due to managerial shortsightedness, as firms often underestimate threats from seemingly unrelated technologies. However, when these connections are established through other new technologies, major companies like Barnes & Noble, Blockbuster, and Kodak collapse.
Article
Multidisciplinary Sciences
Jennifer Cable, Joshua N. Leonard, Timothy K. Lu, Zhen Xie, Matthew Wook Chang, Luis Angel Fernandez, Jose M. Lora, Howard L. Kaufman, Francisco J. Quintana, Roger Geiger, Cammie F. Lesser, Jason P. Lynch, David L. Hava, Virginia W. Cornish, Gary K. Lee, Breanna DiAndreth, Michael Fero, Rajkamal Srivastava, Tim De Coster, Kole T. Roybal, Owen J. L. Rackham, Samira Kiani, Iowis Zhu, Rogelio A. Hernandez-Lopez, Tingxi Guo, William C. W. Chen
Summary: Synthetic biology has the potential to transform cell- and gene-based therapies by engineering cells to selectively respond to disease signals, sparing healthy tissue from potential harm.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2021)
Review
Cell Biology
Gemma Llargues-Sistac, Laia Bonjoch, Sergi Castellvi-Bel
Summary: The use of next-generation sequencing (NGS) technologies has greatly advanced our understanding of the mutational landscape of complex human diseases such as cancer. Haploid human cell models, such as the HAP1 cell line, have emerged as valuable tools for functional gene studies, especially in combination with CRISPR-Cas9 gene editing technology. This review explores the recent applications of the HAP1 cell line in functional genetic studies and high-throughput genetic screens, highlighting its potential to enhance our understanding of gene function and the genetic basis of human diseases identified through NGS technologies, and its implications for clinical practice and patient care.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Junchang Su, Pengju Wang, Ju Li, Dongdong Zhao, Siwei Li, Feiyu Fan, Zhubo Dai, Xiaoping Liao, Zhitao Mao, Chunzhi Zhang, Changhao Bi, Xueli Zhang
Summary: In this study, a simple CRISPR-based genome engineering technique was developed to construct multi-chromosomal E. coli strains without heterologous genetic parts. The successful creation of a new E. coli strain using this technique has important implications for synthetic biology research and applications.
MICROBIAL CELL FACTORIES
(2022)
Article
Biochemistry & Molecular Biology
Hyo-Ju Jang, Hye-Jeong Park, Hong Seok Choi, Hyun Jun Jung, Tae-Hwan Kwon
Summary: This study aimed to establish mpkCCDc14 cells constitutively expressing V2R and AQP2 via CRISPR/Cas9-mediated genome engineering technology. The results showed that these cells had higher abundance of AQP2 protein in the absence of stimulation and exhibited normal response to dDAVP stimulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Xinying Zhang, Shuting Chen, Ying Lin, Wenjie Li, Denggang Wang, Shupeng Ruan, Yuxin Yang, Shuli Liang
Summary: The CRISPR/Cpf1-based gene repression system was developed and optimized for gene editing, which successfully improved lycopene production in Pichia pastoris. Additionally, the sterol regulatory element-binding protein SREBP was used to regulate lipid metabolic pathways, promoting lycopene overproduction. The engineered strain achieved the highest lycopene yield reported to date in P. pastoris.
ACS SYNTHETIC BIOLOGY
(2023)