期刊
MOLECULES
卷 24, 期 14, 页码 -出版社
MDPI
DOI: 10.3390/molecules24142661
关键词
photothermal therapy; phospholipid; gold nanorods; Pseudomonas aeruginosa; biofilm; hydrogel
资金
- Deanship of Scientific Research at The University of Jordan
- Abdul Hameed Shoman Foundation, Amman, Jordan [1820-1104]
- Deanship of Scientific Research and Graduate Studies at Al-Zaytoonah University of Jordan [2017-2016/64/04]
- [3/2017]
In this study, the photothermal-induced bactericidal activity of phospholipid-decorated gold nanorods (DSPE-AuNR) suspension against Pseudomonas aeruginosa planktonic and biofilm cultures was investigated. We found that the treatment of planktonic culture of Pseudomonas aeruginosa with DSPE-AuNR suspension (0.25-0.03 nM) followed by a continuous laser beam exposure resulted in similar to 6 log cycle reduction of the bacterial viable count in comparison to the control. The percentage reduction of Pseudomonas aeruginosa biofilm viable count was similar to 2.5-6.0 log cycle upon laser excitation with different concentrations of DSPE-AuNR as compared to the control. The photothermal ablation activity of DSPE-AuNR (0.125 nM) loaded into poloxamer 407 hydrogel against Pseudomonas aeruginosa biofilm resulted in similar to 4.5-5 log cycle reduction in the biofilm viable count compared to the control. Moreover, transmission electron microscope (TEM) images of the photothermally-treated bacteria revealed a significant change in the bacterial shape and lysis of the bacterial cell membrane in comparison to the untreated bacteria. Furthermore, the results revealed that continuous and pulse laser beam modes effected a comparable photothermal-induced bactericidal activity. Therefore, it can be concluded that phospholipid-coated gold nanorods present a promising nanoplatform to eradicate Pseudomonas aeruginosa biofilm responsible for common skin diseases.
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