4.4 Article

Development of a loop-mediated isothermal amplification assay for Vibrio cholerae O1 and O139

期刊

MOLECULAR AND CELLULAR PROBES
卷 45, 期 -, 页码 65-67

出版社

ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.mcp.2019.05.001

关键词

Cholera; LAMP; O1/O139

资金

  1. Japan Agency for Medical Research and Development
  2. Ministry of Health, Labour and Welfare of Japan

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A loop-mediated isothermal amplification assay was developed. It was designed for recognizing Vibrio cholerae O1/O139, where atpA, rfbN, and wfbR genes were adopted. The assay specifically detected the target with sensitivities of 5-67 copies per reaction in 1 h. The assay will aid rapid detection of the cholera bacterium.

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