Article
Acoustics
Yonggang Wang, Shangchen Sun, Linmiao Yu, Shu Hu, Wenguang Fan, Feifan Leng, Jianzhong Ma
Summary: A transformation system of exogenous DNA into E. coli cells was established in this study through ultrasound mediation. The damage to host cells induced by lower ultrasound power and shorter duration was found to be reversible, with the transformation efficiency and cell survival rate unaffected. Proper changes in cell membrane and wall are essential for exogenous DNA uptake, and the replication and expression of exogenous DNA in cells depend on host cell viability.
ULTRASONICS SONOCHEMISTRY
(2021)
Article
Microbiology
Sara Samperio, Dolores L. Guzman-Herrador, Rigoberto May-Cuz, Maria Cruz Martin, Miguel A. alvarez, Matxalen Llosa
Summary: By using bacterial conjugation as a new tool, genetic modifications have been successfully introduced into many biotechnologically relevant laboratory and wild type lactobacilli, including other genera for which no transformation protocol was previously reported. The study also serendipitously discovered transconjugants in researcher-contaminant Staphylococcus epidermidis.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Green & Sustainable Science & Technology
Y. Zhou, C. Xue, L. Gan, G. Owens, Z. Chen
Summary: In this study, the antibacterial properties of bioreduced graphene oxide (B-rGO) were compared to that of commercial reduced graphene oxide (C-rGO). B-rGO demonstrated good antibacterial activity as confirmed by Scanning Electron Microscopy (SEM). Fourier transform infrared spectroscopy (FTIR) analysis confirmed the presence of numerous oxygen-containing functional groups on the surface of B-rGO. Microcalorimetry and flow cytometry analysis showed that the antibacterial activity of B-rGO was inferior to that of C-rGO.
MATERIALS TODAY SUSTAINABILITY
(2023)
Article
Materials Science, Multidisciplinary
Xintian Shao, Zhou Fang, Miaoling Li, Weiwei Zou, Qianchun Wang, Chengying Zhang, Yongchun Wei, Zhiqi Tian, Yongchao Lai, Yunxia Cao, Baohua Ji, Dechang Li, Qixin Chen, Jiajie Diao
Summary: Graphene oxide (GO)-based nanomaterials have unique properties that make them highly interesting for biomedical research and nanomedicine. This study reveals that GO nanosheets can damage mitochondrial membranes and disrupt mitochondrial cristae, with the size of the nanosheets playing a crucial role. These findings highlight the importance of evaluating the effects of GO-based tools and products on mitochondria.
ACS MATERIALS LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Hanyu Qian, Eric Mclamore, Nikolay Bliznyuk
Summary: Reuse of alternative water sources for irrigation is a sustainable approach to reduce water gaps and increase food production, but it poses a risk of bacterial contamination. This study developed a statistical machine-learning framework to accurately predict the concentration of pathogenic organisms, such as E. coli, in untreated surface water using an impedimetric electrochemical aptasensor. The framework improved prediction accuracy and can be used for rapid decision support in hydroponic irrigation.
Article
Multidisciplinary Sciences
Delia Tomoiaga, Jaclyn Bubnell, Liam Herndon, Paul Feinstein
Summary: Griffith proposed the concept of DNA transfer between bacteria in 1928. Researchers have demonstrated that E. coli can accept multiple plasmids simultaneously, resulting in widespread aclonality during colony development. Tracking clonally related bacteria allows for mosaic analysis of gene function. Additionally, it was discovered that clonally related bacterium rods self-organize fractally and can remain linked during colony growth, presenting a potential target for controlling microbiota growth.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Nishal M. Egodawaththa, Amy L. Knight, Jingxuan Ma, D. Andrew Knight, Eric Guisbert, Nasri Nesnas
Summary: Silver nanoparticles conjugated with organic ligands have been successfully synthesized and demonstrated significant antimicrobial activity and antibacterial capabilities against E. coli. The synthesis of functionalized silver nanoparticles is critical for the further development of silver-nanoconjugated antibacterial agents.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Chia-Hung Chen, Yen-Ping Peng, Ming-Hsun Lin, Ken-Lin Chang, Yung-Chang Lin, Jian Sun
Summary: This study successfully utilized Fe/TNAs to remove E. coli in a photoelectrochemical system, showing higher photocurrent and longer electron lifetime compared to traditional photolysis and photocatalysis methods. Fe/TNAs demonstrated the best removal efficiency for methyl orange degradation in terms of current-light response and electrochemical impedance spectroscopy measurements.
Article
Acoustics
Ziga Pandur, Matevz Dular, Rok Kostanjsek, David Stopar
Summary: This study systematically investigated the resistance of bacterial cells to ultrasonic irradiation by altering the material properties of E. coli cells. The results showed that peptidoglycan, a major component of the cell wall, was the most important determinant of bacterial resistance to sonolysis. Cells without peptidoglycan exhibited high sensitivity to sonolysis.
ULTRASONICS SONOCHEMISTRY
(2022)
Article
Agriculture, Dairy & Animal Science
Frederique Pasquali, Cecilia Crippa, Antonio Parisi, Alex Lucchi, Lucia Gambi, Alessandra Merlotti, Daniel Remondini, Maurizio Stonfer, Gerardo Manfreda
Summary: The aim of this study was to investigate the genetic diversity and antimicrobial resistance of E. coli during enrofloxacin therapy in broilers with colisepticemia. The results showed an increased population diversity after treatment, with an increase in fluoroquinolone resistance in E. coli isolates. Qnr-carrying plasmids did not persist, but two persistent conjugative AMR plasmid clusters were identified. Further studies are needed to confirm plasmid persistence not associated with antimicrobial selective pressure.
Article
Chemistry, Analytical
Jino Fathy, Yongjun Lai
Summary: This paper presents a dynamic-mode microcantilever sensor based on a gap method. By applying ac electric potential and dielectrophoresis force, the sensor can detect E. coli bacteria in real-time, with the concentration and electric potential amplitude affecting the frequency shifts.
Article
Food Science & Technology
Wohab Ali, Kishor Sosmith Utsho, Susmita Karmakar, Nazmul Hoque, Tanvir Rahman, Jayedul Hassan
Summary: This study investigated the prevalence and characteristics of colistin-resistant Escherichia coli (CREC) in broiler meat in Bangladesh. It found that 55.77% of the meat samples were positive for CREC, with 91.38% of the isolates carrying the mcr-1 gene. These CREC isolates were multidrug-resistant and had minimum inhibitory concentrations of 4-8μg/mL for colistin sulfate. Whole genome sequencing revealed that one of the isolates belonged to a potentially pathogenic strain with multiple virulence factor genes.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Engineering, Environmental
Meizhen Wang, Zhangqiang Li, Yunyun Zhang, Yue Li, Na Li, Dan Huang, Baile Xu
Summary: Graphene oxide (GO) exhibits high-efficient antibacterial activity against various pathogenic bacteria. The mechanism of GO's antibacterial properties, particularly its greater adsorption affinity to teichoic acids in the cell walls of Gram-positive bacteria, has been illuminated in this study, providing insights for the potential application of graphene-based materials in water disinfection and pathogen control.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Biotechnology & Applied Microbiology
Mathias Gotsmy, Florian Strobl, Florian Weiss, Petra Gruber, Barbara Kraus, Juergen Mairhofer, Juergen Zanghellini
Summary: This study used constraint-based metabolic modeling to optimize the productivity of plasmid DNA (pDNA) production. By depleting certain nutrients in the growth medium, cell growth was stalled and pDNA production was increased, resulting in improved yield and quality. The findings highlight the importance of controlling nutrient availability for enhancing biotechnological product manufacturing.
MICROBIAL CELL FACTORIES
(2023)
Article
Multidisciplinary Sciences
Zhili Yu, Yaoming Wu, Muyuan Chen, Tong Huo, Wei Zheng, Steven J. Ludtke, Xiaodong Shi, Zhao Wang
Summary: In this study, the authors investigated the secretins GspD(& alpha;) and GspD(& beta;) in Escherichia coli and determined their intermediate structures during the translocation process using Cryo-ET. The obtained structures revealed different membrane interaction patterns and peptidoglycan layer transitions, suggesting two distinct models for the membrane translocation of GspD(& alpha;) and GspD(& beta;). These findings provide valuable insights into the biogenesis of T2SS secretins and their role in bacterial toxin secretion.
NATURE COMMUNICATIONS
(2023)
Article
Materials Science, Ceramics
Tatyana Molodtsova, Mikhail Gorshenkov, Evgeny Kolesnikov, Igor Leontyev, Vasily Kaichev, Denis Zhigunov, Nikita Faddeev, Alexandra Kuriganova, Nina Smirnova
Summary: A series of nano-In2O3 materials with tunable cubic-to-rhombohedral phase ratios were synthesized through pulse alternating current electrosynthesis and air annealing. The synthetic conditions greatly influenced the structural, morphological, and optical properties of In2O3. The c/rh-In2O3 synthesized using NaCl aqueous electrolyte showed enhanced photoelectrochemical activity, photoconversion efficiency, and long-term stability. These results contribute to a better understanding of designing metal oxide phase-junctions for photoelectrochemical applications.
CERAMICS INTERNATIONAL
(2023)
Article
Chemistry, Multidisciplinary
Daria V. Chernysheva, Ekaterina A. Sidash, Maksim S. Konstantinov, Victor A. Klushin, Denis V. Tokarev, Veronica E. Andreeva, Evgeny A. Kolesnikov, Vasily V. Kaichev, Nina V. Smirnova, Valentine P. Ananikov
Summary: Sustainable human development requires reducing the cost of energy storage. The conversion of waste materials into high-value-added carbon materials is well developed for solid wastes, but more challenging for liquid wastes. In this study, a new approach was developed to convert liquid waste into solid materials with nitrogen incorporation. The obtained carbon materials for energy storage exhibited superior characteristics under simple process conditions.
Article
Biochemistry & Molecular Biology
Victor V. Tatarskiy, Olga V. Zakharova, Peter A. Baranchikov, Dmitry S. Muratov, Denis V. Kuznetsov, Alexander A. Gusev
Summary: 2D ultrathin nanomaterials have significant potential in biomedicine, but their cytotoxicity and interactions with other materials are not well understood. In this study, we investigated the effects of graphene oxide (GO) nanolayers and ZrS3 nanoribbons on colon carcinoma cells and found that ZrS3 exhibited strong cytotoxicity while GO reduced its toxicity. This finding demonstrates the ability of graphene oxide to decrease the cytotoxicity of other nanomaterials, which is important in the development of biocompatible nanocoatings and theranostic nanostructures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Forestry
Konstantin V. Zhuzhukin, Peter M. Evlakov, Tatiana A. Grodetskaya, Alexander A. Gusev, Olga V. Zakharova, Aleksey V. Shuklinov, Elena V. Tomina
Summary: Recent studies have found that nanomaterials, especially carbon nanotubes, have various effects on living organisms, ranging from stimulation to toxicity. Plants are important subjects of this research, as carbon nanotubes have potential uses in agriculture and environmental protection. The specific mechanisms of plant resistance to carbon nanotubes, especially in woody plants, are not fully understood. In this study, the effects of aqueous colloids of multi-walled carbon nanotubes (MWCNTs) on morphometric parameters and stress resistance gene expression in two species of birch plants were investigated. Results showed that low concentrations of MWCNTs stimulated plant growth and increased stress resistance gene expression, while high concentrations had negative effects. These findings suggest potential applications of MWCNTs in woody plant biotechnology.
Article
Materials Science, Multidisciplinary
Alexander V. Gorokhovsky, Gleb Yu. Yurkov, Igor N. Burmistrov, Angel F. Villalpando-Reyna, Denis V. Kuznetsov, Alexander A. Gusev, Bekzod B. Khaidarov, Yuri V. Konyukhov, Olga V. Zakharova, Nikolay V. Kiselev
Summary: Pyroxene glass-ceramic enamels based on blast furnace slag and additives were developed for high temperature protective coatings on carbon steel. The coatings exhibited excellent adhesion, low thermal conductivity, high thermal resistance, wear and chemical resistance, and thermal shock resistance. These cost-effective coatings can be used in corrosive atmospheres and abrasive environments at temperatures up to 1100℃.
Article
Pharmacology & Pharmacy
Oksana A. A. Mayorova, Olga I. I. Gusliakova, Ekaterina S. S. Prikhozhdenko, Roman A. A. Verkhovskii, Daniil N. N. Bratashov
Summary: The use of magnetically labeled blood cells as carriers in targeted drug-delivery systems is a new approach that can improve bloodstream delivery strategies. Blood cells possess biocompatibility and safety features, solving the problem of the patient's immune response to foreign carriers. Platelets, with their high efficiency of loading, show promise in developing personalized drug-delivery systems. This article focuses on using platelets decorated with magnetic nanoparticles for targeted drug delivery through the bloodstream, with potential applications in blood-clotting disorders and cancer cell attachment.
Article
Biochemistry & Molecular Biology
Olga V. Zakharova, Valeria V. Belova, Peter A. Baranchikov, Anna A. Kostyakova, Dmitry S. Muratov, Gregory V. Grigoriev, Svetlana P. Chebotaryova, Denis V. Kuznetsov, Alexander A. Gusev
Summary: In this study, the antibacterial activity of TiS3 nanostructures in water and 0.9% NaCl solution suspensions was analyzed. The results showed that freshly prepared water solutions of TiS3 had the strongest toxicity against E. coli, but the antibacterial effect decreased significantly when the solutions were stored for 24 h or when saline solution was used instead of water. The toxicity of TiS3 in water was higher than that of TiO2 nanoparticles, but the opposite results were observed when saline solution was used. Additionally, there was no correlation between the antibacterial activity of water suspensions of nanoribbons and the stability of their colloidal systems, indicating a negligible contribution of aggregation processes to toxicity. In 0.9% NaCl solution suspensions, toxicity increased proportionally with the increase in zeta potential. It was hypothesized that the specificity of toxicity might be associated with the emission of hydrogen sulphide molecules from the surface of nanoribbons, which could either decrease or increase oxidative stress depending on the concentration. However, further investigation is needed to understand the exact underlying mechanisms. These findings highlight the importance of dispersion medium and storage period in the antibacterial activity of TiS3 nanoribbons, and have implications for nanotoxicological studies and the development of antibacterial substances and biomedical applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Sergei Perkov, Mikhail Makhortov, Oleg Grishin, Stanislav Perevoschikov, Ekaterina S. Prikhozhdenko, Daniil Bratashov, Dmitry Gorin
Summary: Hematomas resulting from trauma are common, however the current treatment options are limited. Phototherapy can speed up healing and improve the appearance of damaged tissue, but efficient phototherapy requires accurate determination of chromophore composition in hematoma. The optoacoustic (OA) technique provides high spatial resolution and optical contrast, making it suitable for obtaining this information. In our experiments using gelatin slin phantoms, we demonstrated that the OA technique can monitor bilirubin concentration during photodegradation and distinguish concentrations at different depths. These results suggest that OA monitoring may be used for efficient hematoma phototherapy.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Chemistry, Analytical
Alexander Gorokhovsky, Igor Burmistrov, Denis Kuznetsov, Alexander Gusev, Bekzod Khaydarov, Nikolay Kiselev, Elena Boychenko, Evgeny Kolesnikov, Ksenia Prokopovich
Summary: Low-temperature vitrification in a KNO3-NaNO3-KHSO4-NH4H2PO4 system with various additives was studied to improve the chemical durability of the obtained material. It was found that glass formation was achieved with 4.2-8.4 wt.% Al nitrate admixtures, while the addition of H3BO3 resulted in a glass-matrix composite containing BPO4 crystalline inclusions. Mg nitrate admixtures inhibited vitrification, but allowed the formation of glass-matrix composites with Al nitrate and boric acid. The obtained materials contained nitrate ions and showed increased water resistance, making them suitable for controlled-release fertilizers containing key nutrients (K, P, N, Na, S, B, and Mg).
Article
Chemistry, Multidisciplinary
Svetlana P. Chebotaryova, Olga Zakharova, Alexander A. Gusev, Petr A. Baranchikov, Evgenii A. Kolesnikov, Anastasia S. Yakusheva, Elena Skripnikova, Elena S. Lobakova, Jingliang Xu, Md Asraful Alam, Alexei E. Solovchenko
Summary: The widespread use of CuO nanoparticles (CuO-NP) for various purposes poses increasing environmental hazards to aquatic ecosystems. This study investigated the accumulation ability of the microalga Desmodesmus sp. for CuO-NP and their components, as well as its tolerance to environmentally relevant concentrations of CuO-NP. The results showed that Desmodesmus sp. is relatively tolerant to CuO-NP and has the potential for bioremoval of copper-based nanostructured hazardous micropollutants (HMP) from aquatic environments.
Article
Chemistry, Multidisciplinary
Anastasia Yakusheva, Mohamed Aly-Eldeen, Alexander Gusev, Olga Zakharova, Denis Kuznetsov
Summary: Amino- and carboxyl-functionalized carbon quantum dots (Amino-CQDs) were synthesized through a microwave treatment of a citric acid, ethylenediamine, and EDTA mix. The Amino-CQDs showed promising fluorescence properties and high sensitivity towards copper ions. They also demonstrated stable fluorescence parameters in alkali metal solutions and sea water. A visual sensor based on Amino-CQDs was successfully developed and could register colorimetric effects using computer vision software.
Article
Biochemical Research Methods
Oleg V. Grishin, Natalia A. Shushunova, Daniil N. Bratashov, Ekaterina S. Prikhozhdenko, Roman A. Verkhovskii, Anastasiia A. Kozlova, Arkady S. Abdurashitov, Olga A. Sindeeva, Anatoly S. Karavaev, Danil D. Kulminskiy, Evgeny V. Shashkov, Olga A. Inozemtseva, Valery V. Tuchin
Summary: Photoacoustic flow cytometry is an effective method for detecting alien objects in the bloodstream, and the detection efficiency depends on the parameters of the laser pulse. In this study, the dependence of photoacoustic signals on laser pulse energy, pulse length, and pulse repetition rate for melanoma cells was investigated. The results showed that signal generation efficiency increased with higher laser pulse energy and shorter pulse length, but higher pulse rates could lead to skin overheating. These findings provide important insights for the effective use of photoacoustic flow cytometry in detecting circulating tumor cells.
Correction
Biochemistry & Molecular Biology
Roman Verkhovskii, Alexey Ermakov, Oleg Grishin, Mikhail A. Makarkin, Ilya Kozhevnikov, Mikhail Makhortov, Anastasiia Kozlova, Samia Salem, Valery Tuchin, Daniil Bratashov
Article
Biochemistry & Molecular Biology
Alexander Gorokhovsky, Igor Burmistrov, Denis Kuznetsov, Alexander Gusev, Bekzod Khaidarov, Nikolay Kiselev, Elena Boychenko, Evgeny Kolesnikov, Ksenia Prokopovich, Yuri Konyukhov, Maksim Kravchenko
Summary: Xerogels based on aqueous solutions of urea in potassium silicate liquid glass were produced and analyzed. The dried gels contained three forms of urea: crystals on the surface, fibrous crystals in the matrix, and molecules/ions incorporated into the matrix. Increasing the content of urea in the gel promoted the formation of crystalline forms and increased the diameter of the fiber-shaped crystals. The release rate of urea from the granulated xerogels was determined, showing a slow release up to 120 days.
Article
Engineering, Chemical
Ksenia Valerievna Sukhareva, Igor Anatolievich Mikhailov, Eldar Alievich Mamin, Tatiana Vadimovna Monakhova, Valerii Vladimirovich Kasparov, Evgeny Aleksandrovich Kolesnikov, Anatoly Anatolievich Popov
Summary: This study investigated novel methods for enhancing the stability and resistance of nitrile-butadiene rubber by treating its surface with 1,1,2-trifluoro-1,2,2-trichlorethane. The duration of the modification was found to strongly affect the specific properties of the rubber. Surface modification with 1,1,2-trifluoro-1,2,2-trichlorethane was observed to improve the properties of nitrile-butadiene rubber, and the impact of modification duration on morphology, thermal resistance, and chemical resistance was subsequently presented.
POLYMER ENGINEERING AND SCIENCE
(2023)
Article
Materials Science, Biomaterials
Mohammadreza Kasravi, Alireza Yaghoobi, Tahereh Tayebi, Mahsa Hojabri, Abdolkarim Talebi Taheri, Fatemeh Shirzad, Bahram Jambar Nooshin, Radman Mazloomnejad, Armin Ahmadi, Fatemeh A. Tehrani, Ghasem Yazdanpanah, Mohammad Hadi Farjoo, Hassan Niknejad
Summary: As a promising approach in translational medicine, the decellularization of discarded livers to produce bioscaffolds that support recellularization has potential in overcoming the limitations of conventional liver transplantation. In this study, the researchers investigated the use of matrix metalloproteinase (MMP) inhibition to preserve the extracellular matrix (ECM) during liver decellularization. The results demonstrated that the application of an MMP inhibitor significantly improved the preservation of ECM components and mechanical properties of the bioscaffolds, which supported cell viability and function in vitro. The study also confirmed that the MMP inhibition led to the inhibition of MMP2 and MMP9, providing a novel method to enhance ECM preservation during liver decellularization.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Mohammadsadegh Nadimifar, Weiguang Jin, Clara Coll-Satue, Gizem Bor, Paul Joseph Kempen, Ali Akbar Moosavi-Movahedi, Leticia Hosta-Rigau
Summary: This study presents a metal-phenolic self-assembly approach that can prepare nanoparticles fully made of hemoglobin. The nanoparticles exhibit good oxygen binding and releasing capabilities.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jyoti Kumari, Roel Hammink, Jochem Baaij, Frank A. D. T. G. Wagener, Paul H. J. Kouwer
Summary: Fibrosis is the formation of fibrous connective tissue in response to injury, leading to organ dysfunction. A novel hybrid hydrogel combining synthetic polyisocyanide with hyaluronic acid has been developed, showing strong antifibrotic properties.
BIOMATERIALS ADVANCES
(2024)
Letter
Materials Science, Biomaterials
Melissa Machado Rodrigues, Cristian Padilha Fontoura, Charlene Silvestrin Celi Garcia, Sandro Tomaz Martins, Joao Antonio Pegas Henriques, Carlos Alejandro Figueroa, Mariana Roesch Ely, Cesar Aguzzoli
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jessica Polak, David Sachs, Nino Scherrer, Adrian Suess, Huan Liu, Mitchell Levesque, Sabine Werner, Edoardo Mazza, Gaetana Restivo, Mirko Meboldt, Costanza Giampietro
Summary: Human skin equivalents (HSEs) play a crucial role in tissue engineering. This study introduces a 3D-printed culture insert to apply a static radial constraint on HSEs and examines its effects on tissue characteristics. The results show that the diameter of the culture insert significantly influences tissue contraction, fibroblast and matrix organization, keratinocyte differentiation, epidermal stratification, and basement membrane formation. This study provides important insights for the design of skin tissue engineering.
BIOMATERIALS ADVANCES
(2024)
Review
Materials Science, Biomaterials
Shiliang Chen, Tianming Du, Hanbing Zhang, Jing Qi, Yanping Zhang, Yongliang Mu, Aike Qiao
Summary: This paper reviewed the primary methods for improving the overall properties of biodegradable zinc stents. It discussed the mechanical properties, degradation behavior, and biocompatibility of various improvement strategies. Alloying was found to be the most common, simple, and effective method for improving mechanical properties. Deformation processing and surface modification further improved the mechanical properties and biological activity of zinc alloys. Meanwhile, structural design could endow stents with special properties. Manufacturing zinc alloys with excellent properties and exploring their interaction mechanism with the human body are areas for future research.
BIOMATERIALS ADVANCES
(2024)