Article
Polymer Science
Julian Cremer, Bernhard P. Kaltschmidt, Annika Kiel, Jens Eberhard, Stephan Schmidt, Christian Kaltschmidt, Barbara Kaltschmidt, Andreas Huetten, Dario Anselmetti
Summary: The aging-induced surface-covering EBS layers changed the surface morphology, promoting bacterial adhesion and biofilm formation of Pseudomonas aeruginosa. This study provides insight into the time-dependent, polymer-liquid interface aging process of industrial performance polypropylene samples and highlights the detrimental effects of EBS on surface transformation and degradation.
Article
Immunology
Zuoji Ye, Liumei Ye, Dingbin Li, Shunsheng Lin, Wusheng Deng, Li Zhang, Jinhua Liang, Jinlong Li, Qingjun Wei, Ke Wang
Summary: In this study, the researchers found that Daphnetin (DAP) can effectively inhibit and eradicate biofilm formation of Pseudomonas aeruginosa. DAP also reduced bacterial motility and pyocyanin production. These findings suggest that DAP may serve as a natural anti-biofilm therapeutic agent.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Alexander D. Klementiev, Marvin Whiteley
Summary: Scanning electrochemical probe microscopy methods can provide information of the chemical environment along a spatial surface with micron-scale resolution. These methods often require expensive instruments that perform optimized and highly sensitive niche techniques. The development of an inexpensive electrochemical system, featuring a combined micromanipulator and potentiostat component, allows for spatial measurement of chemical gradients above live bacteria in real time, providing insights into bacterial redox-mediated physiology.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Ehsan Asghari, Annika Kiel, Bernhard Peter Kaltschmidt, Martin Wortmann, Nadine Schmidt, Bruno Huesgen, Andreas Huetten, Cornelius Knabbe, Christian Kaltschmidt, Barbara Kaltschmidt
Summary: New ecological trends and changes in consumer behavior are leading to increased biofilm formation in household appliances, creating a demand for new antimicrobial materials and surfaces. Laboratory-cultivated biofilms identified bacterial strains using MALDI-TOF-MS and analyzed the main genera found in these habitats, such as Staphylococci and pseudomonads, offering better understanding of biofilm formation mechanisms.
Article
Agriculture, Dairy & Animal Science
Daria Plokarz, Michal Czopowicz, Karolina Bierowiec, Krzysztof Rypula
Summary: Pseudomonas aeruginosa is a widespread and opportunistic pathogen in dogs and cats. The ability to form biofilm and the presence of virulence factor genes contribute to the high risk of treatment complications. This study examined the prevalence of virulence factor genes involved in biofilm formation in P. aeruginosa isolates from dogs and cats. The results suggest that the fliC gene and the absence of nan1 gene could indicate the biofilm-forming ability of P. aeruginosa.
Article
Biochemistry & Molecular Biology
J. Pelle, M. Longo, N. Le Poul, C. Hellio, S. Rioual, B. Lescop
Summary: We thoroughly investigated biofilm formation onto glassy carbon electrode surfaces using electrochemical techniques. Pseudomonas aeruginosa was studied due to its remarkable ability to form biofilms in various environments. In-situ measurement of the open-circuit potential was used to monitor the modification of the electrode-solution interface during biofilm growth, which was correlated with results obtained from other techniques such as electrochemical impedance spectroscopy, cyclic voltammetry, scanning electron microscopy, and bioassays. The sensing of biofilm formation suggests a multi-step mechanism that may involve the pre-formation of an insulating layer onto the surface prior to bacteria adhesion and biofilm formation.
BIOELECTROCHEMISTRY
(2023)
Article
Microbiology
Telma de Sousa, Michel Hebraud, Olimpia Alves, Eliana Costa, Luis Maltez, Jose Eduardo Pereira, Angela Martins, Gilberto Igrejas, Patricia Poeta
Summary: A study conducted at the Medical Center of Tras-os-Montes and Alto Douro (CHTMAD) found that Pseudomonas aeruginosa strains causing urinary tract infections are resistant to multiple antibiotics and have high biofilm formation and motility capabilities, posing a serious public health problem.
Article
Immunology
Wusheng Deng, Yanmei Lei, Xiujia Tang, Dingbin Li, Jinhua Liang, Jing Luo, Liuyuan Liu, Wenshu Zhang, Liumei Ye, Jinliang Kong, Ke Wang, Zhaoyan Chen
Summary: This study investigated whether DNase I can inhibit the early formation of biofilms in Pseudomonas aeruginosa- or Staphylococcus aureus-induced empyema models. The results showed that DNase I significantly inhibited early biofilm formation in a dose-dependent manner, without affecting bacterial growth. Therefore, DNase I has therapeutic potential against empyema biofilms.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Immunology
Aref Shariati, Zahra Chegini, Ehsanollah Ghaznavi-Rad, Ehsan Nazarzadeh Zare, Seyed Mostafa Hosseini
Summary: The biofilm community is the dominant mode of microbial growth in nature and contributes to the prevalence of multidrug-resistant strains. Poly lactic-co-glycolic acid (PLGA)-based nanoplatforms show promise in delivering anti-biofilm agents (ABAs) for the destruction of microbial biofilms. However, further research is needed to evaluate the exact interaction between PLGA and biofilms.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Editorial Material
Genetics & Heredity
Tatyana L. Povolotsky, Alona Keren-Paz, Ilana Kolodkin-Gal
Summary: For bacteria, the transition from unicellular entities to multicellular biofilm communities generates distinct metabolic microenvironments. Dynamic and programmed metabolic responses allow the biofilms to react to local changes in nutrient levels and contribute to phenotypic antibiotic resistance of the community. Novel therapeutic approaches targeting biofilms may stem from these metabolic adaptations.
TRENDS IN GENETICS
(2021)
Article
Multidisciplinary Sciences
Eleonora Secchi, Giovanni Savorana, Alessandra Vitale, Leo Eberl, Roman Stocker, Roberto Rusconi
Summary: It has been found that the mechanical stability of Pseudomonas aeruginosa streamers mainly relies on the presence of extracellular DNA (eDNA) and the exopolysaccharide Pel. Controlling the composition of streamers can effectively control the formation of biofilm structures.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Dan Yang, Suqi Hao, Ling Zhao, Fei Shi, Gang Ye, Yuanfeng Zou, Xu Song, Lixia Li, Zhongqiong Yin, Xiaoli He, Shiling Feng, Helin Chen, Yu Zhang, Yuanze Gao, Yinglun Li, Huaqiao Tang
Summary: The study found that paeonol has good anti-biofilm activity against Gram-negative bacteria and anti-QS activity, effectively reducing the virulence of P. aeruginosa. In vivo studies showed that paeonol could reduce the pathogenicity of P. aeruginosa and enhance the survival rate of C. elegans.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Chemistry, Analytical
Noemi Poma, Federico Vivaldi, Andrea Bonini, Pietro Salvo, Arno Kirchhain, Zeliha Ates, Bernardo Melai, Daria Bottai, Arianna Tavanti, Fabio Di Francesco
Summary: Future strategies for detecting and monitoring biofilm formation in both industry and human health are crucial due to the negative impact of biofilms. Various electrochemical techniques including potentiometric, voltammetric, and impedance-based sensors are being used to provide an overview of biofilm detection and monitoring processes.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Microbiology
Bernhard Peter Kaltschmidt, Ehsan Asghari, Annika Kiel, Julian Cremer, Dario Anselmetti, Christian Kaltschmidt, Barbara Kaltschmidt, Andreas Huetten
Summary: This study investigated the application of magnetron sputtering of various transition metals in antimicrobial coatings, finding that gold had the strongest antibacterial effect. These antimicrobial coatings show great potential for application on household appliances and surgical instruments.
Article
Infectious Diseases
Mario Gajdacs, Zoltan Barath, Krisztina Karpati, Dora Szabo, Donatella Usai, Stefania Zanetti, Matthew Gavino Donadu
Summary: The study found a relationship between biofilm formation and expression of virulence factors with multidrug resistance in Pseudomonas aeruginosa. However, there were no significant differences in virulence factor expression between multidrug-resistant and non-multidrug-resistant isolates, and no correlations were seen between the rate of biofilm formation, pigment production, or motility. Understanding the interplay between drug resistance mechanisms, biofilm formation, and virulence is crucial for managing chronic bacterial infections.
Article
Chemistry, Medicinal
Yasmine Radwan, Kholoud K. Arafa, Monira G. Ghoniem, Eida S. Al-Farraj, Ibrahim M. El-Sherbiny
Summary: This study aimed to investigate the synergistic therapeutic effect of the novel combination of capsaicin and 5-fluorouracil encapsulated in lipid nanocapsules. The results showed that the developed nanoformulations had good physical stability and exhibited superior antiproliferative effect compared to the free drugs.
DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY
(2023)
Article
Pharmacology & Pharmacy
Mohamed El -Far, Alaa Essam, Fardous F. El-Senduny, Amira O. Abd El-Azim, Sarah Yahia, Ibrahim M. El-Sherbiny
Summary: In this study, the anticancer effect of a novel drug combination using nanoformulations of Quercetin and Ascorbyl palmitate was investigated in Ehrlich ascites carcinoma (EAC)-bearing mice. The combination treatment significantly reduced tumor growth, increased antioxidant status, and reduced lipid peroxidation. The combination also downregulated the expression of p-STAT3 and HIF-1 alpha, induced cell cycle arrest and apoptosis in EAC cells, and caused DNA damage. The combination was also superior in the destruction of the mitochondrial membrane.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Mohamed Fawzi Kabil, Osama A. Badary, Frank Bier, Shaker A. Mousa, Ibrahim M. El-Sherbiny
Summary: Cancer is a major cause of death worldwide, and chemotherapy is the most commonly used treatment. However, current chemotherapeutic treatments have limitations, including lack of specificity and adverse effects. Lipid nanoparticles (LNPs) offer a promising nanocarrier system to improve drug delivery and reduce side effects, potentially leading to better therapeutic outcomes.
JOURNAL OF LIPOSOME RESEARCH
(2023)
Article
Multidisciplinary Sciences
Heba A. Hussein, Amro Hanora, Samar M. Solyman, Rabeay Y. A. Hassan
Summary: A designed signature probe based on nanostructured-AuNPs/WO3 screen printed electrodes was immobilized for rapid diagnostic of SARS-CoV-2. The detection limits and quantification limits were optimized to 298 and 994 fM respectively. The study also confirmed the hybridization of the probe with the virus genome in 5 minutes at room temperature, making it a rapid tool for SARS-CoV-2 detection.
SCIENTIFIC REPORTS
(2023)
Correction
Pharmacology & Pharmacy
Amira Mansour, Mohamed Y. Mahmoud, Alaa F. Bakr, Monira G. Ghoniem, Fatima A. Adam, Ibrahim M. El-Sherbiny
Article
Chemistry, Multidisciplinary
Mostafa Fytory, Amira Mansour, Waleed M. A. El Rouby, Ahmed A. Farghali, Xiaorong Zhang, Frank Bier, Mahmoud Abdel-Hafiez, Ibrahim M. El-Sherbiny
Summary: By grafting glycyrrhetinic acid to polyethyleneimine, a biocompatible Zr-based metal-organic framework was designed for efficient controlled drug release and selective targeting against hepatic cancer cells. The improved core-shell structure enhanced the drug loading capacity and selectivity towards tumor cells, while exhibiting minimal toxicity to normal cells. This study provides a new approach for active drug delivery and efficient cancer therapy applications.
Article
Biophysics
Aaditya S. Deshpande, Wayne Muraoka, James Wait, Arzu Colak, Silvana Andreescu
Summary: This study presents the design and characterization of a robust biosensor for real-time monitoring of superoxide (O-2(center dot-)) release. The biosensor is constructed by depositing a uniform layer of LCysteine on a gold wire electrode and conjugating it with Cytochrome C (Cyt c). The biosensor demonstrates stability and robustness in complex biological systems and shows potential for estimating oxidative injury in human pathologies.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Electrochemistry
Rabee H. R. Mohammed, Rabeay Y. A. Hassan, Rehab Mahmoud, Ahmed A. Farghali, Mohamed E. M. Hassouna
Summary: Nickel tungstate/multi-walled carbon nanotubes composite was successfully utilized as a carbon paste electrode modifier for the simple and rapid analysis of cadmium ion in environmental and biological samples. The composite was fully characterized and the optimal conditions for the electro-analysis assay were determined. A linear relation of cadmium ions in the concentration range of 50-450 μM was achieved.
JOURNAL OF APPLIED ELECTROCHEMISTRY
(2023)
Article
Chemistry, Analytical
Hend S. Magar, Heba Abdelghany, Mohammed Nooredeen Abbas, Ursula Bilitewski, Rabeay Y. A. Hassan
Summary: Rapid analysis and early detection of food poisoning is crucial for food safety and public health. A label-free impedimetric immunosensor was developed for the detection and identification of S. aureus, which showed high sensitivity and selectivity. The immunosensor was successfully used to analyze S. aureus contamination in milk and meat samples.
MICROCHEMICAL JOURNAL
(2023)
Article
Nanoscience & Nanotechnology
Heba A. Hussein, Ahmed Kandeil, Mokhtar Gomaa, Rabeay Y. A. Hassan
Summary: To promote public health interventions, the priority is to diagnose COVID-19 rapidly and reliably. Double-antibody-based immunobiosensor chips were designed and constructed for clinical diagnosis. These chips showed high sensitivity and selectivity for detecting the SARS-CoV-2 virus and its variants, even in the presence of other coronaviruses. The immunobiosensor was validated with clinical specimens and can provide instantaneous detection within 15 minutes.
MICROSYSTEMS & NANOENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Reham G. Elfarargy, Mohamed Sedki, Farag A. Samhan, Rabeay Y. A. Hassan, Ibrahim M. El-Sherbiny
Summary: This study successfully reduced the formation of pathogenic biofilms by chemically modifying polyurethane materials. This promising proof-concept opens up new directions for producing better and sustainable stents and catheters biomaterials.
JOURNAL OF GENETIC ENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Nadeen Rajab, Hosny Ibrahim, Rabeay Y. A. Hassan, Ahmed F. A. Youssef
Summary: An electrochemical assay using disposable nano-sensor chip ZrO2@MWCNTs screen printed electrodes (SPE) was constructed for the rapid, selective, and sensitive determination of nitrite in food and water samples. The use of nanomaterials as a sensing platform exhibited superior electrocatalytic activity and conductivity. Morphological, structural, and electrochemical analyses were performed to study the performance of the sensor. The assay showed a wide linear range and high selectivity, and achieved a high recovery rate in real sample analysis.
Article
Chemistry, Multidisciplinary
Sarah Yahia, Islam A. Khalil, Monira G. Ghoniem, Ibrahim M. El-Sherbiny
Summary: This work aimed to develop a functional spine unit (FSU) replacement using different technologies to mimic the structure and function of the vertebral body (VB) and intervertebral disc (IVD) and treat spinal tuberculosis (TB). 3D-printed scaffolds with different porous patterns were fabricated to replace damaged VB and IVD. The results showed excellent mechanical strength and biocompatibility of the custom-designed replacements, along with the expected prolonged release of antibiotics. These findings suggest that this drug-eluting scaffold system could be used for treating various spinal problems.
Article
Pharmacology & Pharmacy
Amira Mansour, Mostafa Fytory, Osama M. Ahmed, Fatema El-Zahraa S. Abdel Rahman, Ibrahim M. El-Sherbiny
Summary: This study developed a novel nanostructure for breast cancer treatment by loading Methotrexate (MTX) and 5-fluorouracil (Fu) into a core-shell nanocarrier. The nanostructure showed high drugs' entrapment efficiency and a pH-responsive drug release pattern. In vitro and in vivo experiments demonstrated the enhanced anticancer effect of the developed nanostructure and its ability to maintain the normal functions of vital organs.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Chemistry, Multidisciplinary
Ahmed S. Abo Dena, Mariam Dhaou, Ibrahim M. El-Sherbiny
Summary: In this study, a fluorescent molecularly imprinted polymer (FMIP) was developed for the solid-phase extraction (SPE) and fluorimetric determination of hydrochlorothiazide (HCTZ) in water. The FMIP exhibited high adsorption capacity and selectivity for HCTZ, making it suitable for easy and fast SPE of HCTZ.
Article
Materials Science, Biomaterials
Mohammadreza Kasravi, Alireza Yaghoobi, Tahereh Tayebi, Mahsa Hojabri, Abdolkarim Talebi Taheri, Fatemeh Shirzad, Bahram Jambar Nooshin, Radman Mazloomnejad, Armin Ahmadi, Fatemeh A. Tehrani, Ghasem Yazdanpanah, Mohammad Hadi Farjoo, Hassan Niknejad
Summary: As a promising approach in translational medicine, the decellularization of discarded livers to produce bioscaffolds that support recellularization has potential in overcoming the limitations of conventional liver transplantation. In this study, the researchers investigated the use of matrix metalloproteinase (MMP) inhibition to preserve the extracellular matrix (ECM) during liver decellularization. The results demonstrated that the application of an MMP inhibitor significantly improved the preservation of ECM components and mechanical properties of the bioscaffolds, which supported cell viability and function in vitro. The study also confirmed that the MMP inhibition led to the inhibition of MMP2 and MMP9, providing a novel method to enhance ECM preservation during liver decellularization.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Mohammadsadegh Nadimifar, Weiguang Jin, Clara Coll-Satue, Gizem Bor, Paul Joseph Kempen, Ali Akbar Moosavi-Movahedi, Leticia Hosta-Rigau
Summary: This study presents a metal-phenolic self-assembly approach that can prepare nanoparticles fully made of hemoglobin. The nanoparticles exhibit good oxygen binding and releasing capabilities.
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jyoti Kumari, Roel Hammink, Jochem Baaij, Frank A. D. T. G. Wagener, Paul H. J. Kouwer
Summary: Fibrosis is the formation of fibrous connective tissue in response to injury, leading to organ dysfunction. A novel hybrid hydrogel combining synthetic polyisocyanide with hyaluronic acid has been developed, showing strong antifibrotic properties.
BIOMATERIALS ADVANCES
(2024)
Letter
Materials Science, Biomaterials
Melissa Machado Rodrigues, Cristian Padilha Fontoura, Charlene Silvestrin Celi Garcia, Sandro Tomaz Martins, Joao Antonio Pegas Henriques, Carlos Alejandro Figueroa, Mariana Roesch Ely, Cesar Aguzzoli
BIOMATERIALS ADVANCES
(2024)
Article
Materials Science, Biomaterials
Jessica Polak, David Sachs, Nino Scherrer, Adrian Suess, Huan Liu, Mitchell Levesque, Sabine Werner, Edoardo Mazza, Gaetana Restivo, Mirko Meboldt, Costanza Giampietro
Summary: Human skin equivalents (HSEs) play a crucial role in tissue engineering. This study introduces a 3D-printed culture insert to apply a static radial constraint on HSEs and examines its effects on tissue characteristics. The results show that the diameter of the culture insert significantly influences tissue contraction, fibroblast and matrix organization, keratinocyte differentiation, epidermal stratification, and basement membrane formation. This study provides important insights for the design of skin tissue engineering.
BIOMATERIALS ADVANCES
(2024)
Review
Materials Science, Biomaterials
Shiliang Chen, Tianming Du, Hanbing Zhang, Jing Qi, Yanping Zhang, Yongliang Mu, Aike Qiao
Summary: This paper reviewed the primary methods for improving the overall properties of biodegradable zinc stents. It discussed the mechanical properties, degradation behavior, and biocompatibility of various improvement strategies. Alloying was found to be the most common, simple, and effective method for improving mechanical properties. Deformation processing and surface modification further improved the mechanical properties and biological activity of zinc alloys. Meanwhile, structural design could endow stents with special properties. Manufacturing zinc alloys with excellent properties and exploring their interaction mechanism with the human body are areas for future research.
BIOMATERIALS ADVANCES
(2024)