期刊
JOURNAL OF SEPARATION SCIENCE
卷 42, 期 16, 页码 2706-2714出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.201900258
关键词
hyper-cross-linked polysturene; phenylcarboxylic acids; restricted access materials; solid-phase extraction
The study was focused on the development of a solid-phase extraction protocol for seven phenylcarboxylic acids from albumin solutions by using unmodified hypercross- linked polystyrene restricted access materials with crosslinking degrees varying from 100 to 400% (four of the acids are known to be markers of sepsis). The breakthrough volume of the most hydrophilic 3,4-dihydroxybenzoic acid rises as the sorbent bridging extent grows. Inversely, the breakthrough volume of themost hydrophobic 3-phenylpropionic acid was found to decrease considerably when the degree of crosslinking exceeds 200%. This unusual pattern is because of the superposition of two opposite tendencies. Increasing substitution extent of phenyls facilitates their p-p-interactions with polar compounds whereas rising density of the network reduces the accessibility of sorption sites to all solutes. Mini-cartridges containing 30 mg of an optimal sorbent take up the acids completely and reversibly, the recoveries being close to 100% even in the presence of high concentrations of albumin. By coupling the developed solid-phase extraction with high-performance liquid chromatography and diode array detection technique, we managed to determine quantitatively phenylcarboxylic acids in the serum of a healthy patient blood, and the recoveries varied from 93 to 100% while the limit of quantification was (4-9) x 10-7 M.
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