Article
Biochemical Research Methods
Yuhao Qiang, Jia Liu, Ming Dao, E. Du
Summary: Red blood cells are exposed to varying shear stress and oxygen tension during circulation, which can affect their biomechanics. Cyclic hypoxia alone significantly reduces cell deformability, particularly in sickle cells which show less resistance to fatigue damage. Treatment with an anti-sickling drug can improve the fatigue resistance of sickle cells exposed to cyclic hypoxia.
Article
Biochemistry & Molecular Biology
Sarah Rouge, Sandrine Genetet, Maria Florencia Leal Denis, Michael Dussiot, Pablo Julio Schwarzbaum, Mariano Anibal Ostuni, Isabelle Mouro-Chanteloup
Summary: The study reveals that stomatin plays a major role in modulating PANX1 activity in red blood cells and its absence leads to significant defects in PANX1 function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Brian S. Ferguson, Leslie E. Neidert, Matthew J. Rogatzki, Keith R. Lohse, L. Bruce Gladden, Heidi A. Kluess
Summary: The study found no significant difference in the release of ATP from RBCs of different densities under hypoxic and normoxic conditions, and the release of ATP was linearly correlated with the release of Hb.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Brianna M. Vickerman, Colin P. O'Banion, Xianming Tan, David S. Lawrence
Summary: Protein therapeutics have high selectivity and potency, but are often limited by short half-lives and off-target effects. This study introduces a targeted protein delivery system using engineered red blood cells and light-triggered hemolysis for drug release. The strategy shows spatiotemporal control and successful delivery of therapeutic proteins in vitro and in vivo.
ACS CENTRAL SCIENCE
(2021)
Article
Immunology
Robson Xavier Faria
Summary: The inflammatory focus and tumor microenvironment share similarities in terms of immune cells and macrophages. Under simulated hypoxic conditions, the function of P2X7 receptors is enhanced, along with increased HIF-1 alpha levels and suppressed HIF-1 alpha antagonists. Additionally, the intracellular P2X7 receptor regulator PIP2 is activated in simulated hypoxic conditions.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Physiology
Fatih Celal Alcicek, Tasnim Mohaissen, Katarzyna Bulat, Jakub Dybas, Ewa Szczesny-Malysiak, Magdalena Kaczmarska, Magdalena Franczyk-Zarow, Renata Kostogrys, Katarzyna M. Marzec
Summary: For the first time, this study investigated the correlation between sex-specific differences in ATP levels in red blood cells and their mechanical, biochemical, and morphological alterations during the progression of atherosclerosis in mice. The results showed that sex and age have an impact on the alterations in red blood cells, and female red blood cells exhibit lower ATP levels and higher hemoglobin concentration, mean corpuscular volume, mean corpuscular hemoglobin, deformability, and phosphatidylserine exposure levels compared to male red blood cells. Changes in ATP levels were correlated with phosphatidylserine exposure and mean corpuscular volume.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Chemistry, Analytical
Elizabeth A. Hayter, Samuel Azibere, Lauren A. Skrajewski, Logan D. Soule, Dana M. Spence, R. Scott Martin
Summary: In this paper, a 3D-printed multi-modal device was developed to detect NO and ATP in red blood cells. The device achieved simultaneous detection of NO and ATP using amperometry and chemiluminescence reactions, respectively. The optimized device demonstrated high sensitivity and low limits of detection.
ANALYTICAL METHODS
(2022)
Article
Physiology
Antonella Antonelli, Emanuele Salvatore Scarpa, Mauro Magnani
Summary: This study investigated the effect of oxygen tension on stem and progenitor cell proliferation, differentiation, and cytokine expression in the bone marrow hematopoietic niche. Results showed that the presence of red blood cells up-regulated IL-8 mRNA while IL-6 and VEGF mRNA expression appeared down-regulated, and administration of RBCs led to an increase in TNF alpha expression levels in MonoMac 6 cells, suggesting a potential hypoxia-independent modulation of these factors. Further studies should explore these preliminary observations in more detail considering the role of oxygen in the hematopoietic niche.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Neurosciences
Matthew L. Racine, Janee D. Terwoord, Nathaniel B. Ketelhut, Nate P. Bachman, Jennifer C. Richards, Gary J. Luckasen, Frank A. Dinenno
Summary: The haemodynamic and ATP responses in older adults during hypoxia and exercise are impaired compared to young adults, which is likely due to reduced red blood cell ATP release. Rho-kinase inhibition improves ATP release from isolated red blood cells in older adults. This study tested whether treating older adults with a Rho-kinase inhibitor (fasudil) could improve local haemodynamic and ATP responses during hypoxia and exercise. The results showed that Rho-kinase inhibition improved blood flow and ATP responses in older adults, suggesting that it may have therapeutic implications for age-related impairments in haemodynamics and ATP responses.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Article
Environmental Sciences
Wenxia Zhang, John P. Dunne, Hui Wu, Feng Zhou
Summary: The coastal region in east China is facing massive anthropogenic eutrophication and seasonal hypoxia threat. Future climate changes will worsen the current hypoxia by exacerbating anthropogenic eutrophication. The disappearance of the Yellow Sea Cold Water Mass and the strengthening of shelf vertical stratification are projected by the end of the 21st century.
ENVIRONMENTAL RESEARCH LETTERS
(2022)
Article
Environmental Sciences
Ehsan Abedi, Jafar Seyfabadi, Abolfazl Saleh, Alireza Sari
Summary: This study assessed the impact of near-hypoxic and hypoxic conditions on the mesozooplankton community in the Persian Gulf and the Gulf of Oman. The results showed that the physicochemical parameters significantly explained the variation of mesozooplankton composition in the study areas.
MARINE POLLUTION BULLETIN
(2022)
Article
Fisheries
James J. Pierson, Jeremy M. Testa, Michael R. Roman
Summary: The study utilizes water quality monitoring data to analyze the seasonal and inter-annual variability in habitat for the copepod Acartia tonsa in Chesapeake Bay. Results show that the P-crit metric predicts the largest volume of unsuitable deoxygenated habitat over space and time, with dry conditions leading to a decreased extent of deoxygenated habitat. Although no clear relationship between copepod abundance and habitat availability was observed, the peak abundance of A. tonsa correlates with the extent of deoxygenated habitat using the P-crit metric.
ICES JOURNAL OF MARINE SCIENCE
(2022)
Article
Hematology
Sandra C. Becerra, Barbara A. Christy, Maryanne C. Herzig, James A. Bynum, Daniel N. Darlington
Summary: Experimental results show that even if exposed to radiation, the energy metabolome of red blood cells in cold stored whole blood is not significantly affected, indicating that it can still be used for resuscitation in trauma patients.
Article
Biodiversity Conservation
Stephen F. Jane, Joshua L. Mincer, Maximilian P. Lau, Abigail S. L. Lewis, Jonathan T. Stetler, Kevin C. Rose
Summary: The concentration of dissolved oxygen is important for aquatic ecosystems. A study on lakes found that most lakes have experienced summertime increases in the amount of water below ecologically important threshold concentrations, indicating a reduction in habitat and potential changes in lake chemistry. These trends are expected to be exacerbated by future warming.
GLOBAL CHANGE BIOLOGY
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Yonni Friedlander, Brandon Zanette, Andras A. Lindenmaier, Jordan Fliss, Daniel Li, Kiarash Emami, Robert P. Jankov, Andrea Kassner, Giles Santyr
Summary: In healthy rats, the dissolved Xe-129 chemical shift in red blood cells showed an increase with decreasing fraction of inhaled oxygen (FiO(2)), while the chemical shift in blood plasma and/or tissue did not depend on FiO(2). Additionally, the dissolved Xe-129 chemical shifts were not significantly different under different levels of inhaled carbon dioxide (FiCO(2)).
MAGNETIC RESONANCE IN MEDICINE
(2021)
Article
Neurosciences
Christopher M. Hearon, Jennifer C. Richards, Mathew L. Racine, Gary J. Luckasen, Dennis G. Larson, Michael J. Joyner, Frank A. Dinenno
JOURNAL OF PHYSIOLOGY-LONDON
(2017)
Article
Neurosciences
Matthew L. Racine, Anne R. Crecelius, Gary J. Luckasen, Dennis G. Larson, Frank A. Dinenno
JOURNAL OF PHYSIOLOGY-LONDON
(2018)
Article
Neurosciences
Christopher M. Hearon, Jennifer C. Richards, Mathew L. Racine, Gary J. Luckasen, Dennis G. Larson, Frank A. Dinenno
JOURNAL OF PHYSIOLOGY-LONDON
(2019)
Article
Physiology
Gabrielle A. Dillon, John R. A. Shepherd, Darren P. Casey, Frank A. Dinenno, Timothy B. Curry, Michael J. Joyner, Sushant M. Ranadive
EXPERIMENTAL PHYSIOLOGY
(2020)
Article
Neurosciences
Janee D. Terwoord, Christopher M. Hearon, Matthew L. Racine, Nathaniel B. Ketelhut, Gary J. Luckasen, Jennifer C. Richards, Frank A. Dinenno
JOURNAL OF PHYSIOLOGY-LONDON
(2020)
Article
Neurosciences
Christopher M. Hearon, Jennifer C. Richards, Mathew L. Racine, Gary J. Luckasen, Dennis G. Larson, Frank A. Dinenno
JOURNAL OF PHYSIOLOGY-LONDON
(2020)
Article
Neurosciences
Matthew L. Racine, Janee D. Terwoord, Nathaniel B. Ketelhut, Nate P. Bachman, Jennifer C. Richards, Gary J. Luckasen, Frank A. Dinenno
Summary: The haemodynamic and ATP responses in older adults during hypoxia and exercise are impaired compared to young adults, which is likely due to reduced red blood cell ATP release. Rho-kinase inhibition improves ATP release from isolated red blood cells in older adults. This study tested whether treating older adults with a Rho-kinase inhibitor (fasudil) could improve local haemodynamic and ATP responses during hypoxia and exercise. The results showed that Rho-kinase inhibition improved blood flow and ATP responses in older adults, suggesting that it may have therapeutic implications for age-related impairments in haemodynamics and ATP responses.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)