Article
Biochemistry & Molecular Biology
Jose Manuel Fernandez-Garcia, Beatriz Carrillo, Patricia Tezanos, Paloma Collado, Helena Pinos
Summary: The study found that administration of genistein during early life may delay the emergence of sex differences in body weight and lead to a significant decrease in POMC-ir cells in female rats. However, genistein did not affect orexin expression in either males or females.
Article
Biochemical Research Methods
Sebastian Junge, Felix Schmieder, Philipp Sasse, Jurgen Czarske, Maria Leilani Torres-Mapa, Alexander Heisterkamp
Summary: Controlling and reading the electrical activity in a cardiac syncytium using optical approaches can enhance our understanding of cardiac electrophysiology. In this study, we demonstrate the precise optogenetic stimulation of cardiomyocytes using light foci generated with a spatial light modulator. Additionally, we show that probing the cardiac cells with multiple light foci enhances the excitability of the cardiac network.
JOURNAL OF BIOPHOTONICS
(2022)
Editorial Material
Biochemistry & Molecular Biology
Monica Imbernon, Benedicte Dehouck, Vincent Prevot
Summary: The suprachiasmatic nucleus plays a crucial role in synchronizing physiology with the individual's environment, while tanycytes regulate the circadian changes in brain glucose entry and glycemia under the tempo set by the SCN.
Article
Dermatology
Shivam A. Zaver, Christopher J. Johnson, Andre Berndt, Cory L. Simpson
Summary: Barrier tissues, like the epidermis, use complex signal transduction systems to perform morphogenetic programs and respond to environmental cues. Recent advancements in live-imaging techniques have allowed for precise monitoring and manipulation of signaling cascades using genetically encoded fluorescent biosensors and optogenetic protein constructs. This review paper discusses the principles behind these tools and how they have been used in cutaneous biology to study epidermal development, barrier formation, and tissue homeostasis.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2023)
Article
Medicine, Research & Experimental
Tori Lhomme, Jerome Clasadonte, Monica Imbernon, Daniela Fernandois, Florent Sauve, Emilie Caron, Natalia da Silva Lima, Violeta Heras, Ines Martinez-Corral, Helge Mueller-Fielitz, Sowmyalakshmi Rasika, Markus Schwaninger, Ruben Nogueiras, Vincent Prevot
Summary: Tanycytes in the hypothalamus convert brain glucose into lactate and transmit it to proopiomelanocortin neurons, driving their activity and metabolic response. This transmission relies on the formation of metabolic networks and plays a key role in regulating feeding behavior and energy metabolism.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Endocrinology & Metabolism
Guangpin Chu, Hualing Peng, Nana Yu, Yuejin Zhang, Xueling Lin, Yisheng Lu
Summary: The overexpression of LEAP2 in the hypothalamic arcuate nucleus reduces food intake and body weight, improves metabolic disorders, and increases the expression of POMC. Central delivery of LEAP2 leads to appetite suppression and weight reduction.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
J. Wang, A. Webster, W. Sims, E. R. Gilbert, M. A. Cline
Summary: The central administration of adrenomedullin (AM) induces anorexigenic effects in Japanese quail, possibly through the synergistic effect of proopiomelanocortin (POMC) and cocaine- and amphetamine-regulated transcript (CART) in the arcuate nucleus (ARC).
DOMESTIC ANIMAL ENDOCRINOLOGY
(2021)
Review
Physiology
Jonathan S. Baillie, Matthew R. Stoyek, T. Alexander Quinn
Summary: Optogenetics, as a powerful experimental technique for probing (patho-)physiological function, has been utilized in cardiac research for over a decade. The zebrafish, with its genetic and functional cardiac similarities to mammals, transparent embryo for in vivo visualization, and high-throughput capabilities, represents an experimental model with unrealized potential for cardiac optogenetic studies.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Neurosciences
Dimitrios Firfilionis, Frances Hutchings, Reza Tamadoni, Darren Walsh, Mark Turnbull, Enrique Escobedo-Cousin, Richard G. Bailey, Johannes Gausden, Aaliyah Patel, Dorian Haci, Yan Liu, Fiona E. N. LeBeau, Andrew Trevelyan, Timothy G. Constandinou, Anthony O'Neill, Marcus Kaiser, Patrick Degenaar, Andrew Jackson
Summary: Neuromodulation is an established treatment for neurological conditions, but improving the specificity of stimulation is necessary. Optogenetics offers promising research potential for optical stimulation of specific cell types. An open-source system is being developed for closed-loop optogenetic neuromodulation, allowing for flexibility and expansion in various neuroscientific applications.
FRONTIERS IN NEUROSCIENCE
(2021)
Article
Multidisciplinary Sciences
Leonardo Barneschi, Emanuele Marsili, Laura Pedraza-Gonzalez, Daniele Padula, Luca De Vico, Danil Kaliakin, Alejandro Blanco-Gonzalez, Nicolas Ferre, Miquel Huix-Rotllant, Michael Filatov, Massimo Olivucci
Summary: In this study, quantum chemical models were used to investigate the fluorescence properties of a set of Archaerhodopsin reporters. It was found that the fluorescence intensity is directly proportional to the stability of an exotic excited-state diradical intermediate. This finding is significant for the search for novel fluorescent rhodopsin variants and the understanding of electrostatic changes that enhance light emission.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Rakhat Yermek, Lei Wang, Kentaro Kaneko, Wanxin Han, Yutaka Seino, Daisuke Yabe, Toshihiko Yada
Summary: D-Allulose acts on ARC neurons, including POMC neurons, in cooperation with GLP-1 to reduce food intake, which may contribute to counteracting obesity and diabetes.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Roldan Medina de Guia, Anna S. Hassing, Tao Ma, Kaja Plucinska, Birgitte Holst, Zach Gerhart-Hines, Brice Emanuelli, Jonas T. Treebak
Summary: Deletion of Nampt in AgRP neurons causes progressive neurodegeneration and impairs responses to fasting and ghrelin in a context-dependent manner.
Article
Chemistry, Analytical
Saeyeong Jeon, Youjin Lee, Daeho Ryu, Yoon Kyung Cho, Yena Lee, Sang Beom Jun, Chang-Hyeon Ji
Summary: Optogenetics has become crucial in neuroscience research due to its cell-type-specific neuromodulation feature. Technological advances in light delivery devices have allowed for the combination of optogenetics and electrophysiology, as demonstrated by a novel optrode array design for optical modulation and electrophysiological recording. In vivo experiments with transgenic mice expressing channelrhodopsin have confirmed the effectiveness of optical activation and neural recording.
Article
Multidisciplinary Sciences
Andrii A. Kaberniuk, Mikhail Baloban, Mikhail V. Monakhov, Daria M. Shcherbakova, Vladislav V. Verkhusha
Summary: Researchers have developed a smaller single-component near-infrared system, iLight, derived from a bacterial phytochrome, for controlling gene transcription in bacterial and mammalian cells. The system shows significant repression in bacterial protein production and higher activation in mammalian cells, as well as efficient induction of fluorescent reporter in neurons.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Yunhui Liu, Lu Zhang, Nan Hu, Jie Shao, Dazhi Yang, Changshun Ruan, Shishu Huang, Liping Wang, William W. Lu, Xinzhou Zhang, Fan Yang
Summary: A new optogenetic approach was used to inhibit PTH secretion in human parathyroid cells and prevent bone loss in mice, providing a strategy to regulate PTH release and associated bone loss.
NATURE COMMUNICATIONS
(2022)
Article
Neurosciences
Philip D. Fox, Shane T. Hentges
JOURNAL OF NEUROSCIENCE
(2017)
Article
Neurosciences
Andrew R. Rau, Shane T. Hentges
JOURNAL OF NEUROSCIENCE
(2017)
Article
Physiology
Marissa J. Metz, Caitlin M. Daimon, Connie M. King, Andrew R. Rau, Shane T. Hentges
Summary: This study found diverse projections of POMC neurons in the arcuate nucleus, with a subpopulation projecting to multiple regions simultaneously. Increasing POMC neuron activity did not lead to an increase in synaptic connections with downstream regions. Subpopulations of ARH POMC neurons with distinct efferent projections may serve as a way for the POMC population to organize its many functions.
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
(2021)
Article
Physiology
Caitlin M. Daimon, Shane T. Hentges
Summary: Inhibition of POMC neurons reduces the severity of activity-based anorexia (ABA) without affecting body weight or food intake. POMC neurons may contribute to excessive exercise habits in patients with AN, while metabolic control during ABA appears to occur through mechanisms independent of POMC neurons.
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
(2022)