期刊
APPLIED SOIL ECOLOGY
卷 100, 期 -, 页码 8-17出版社
ELSEVIER
DOI: 10.1016/j.apsoil.2015.11.009
关键词
Nitrifiers; Paddy; q-PCR; T-RFLP; Ammonia monooxygenase
类别
资金
- Consejo Sectorial de Investigacion Cientifica (CSIC-Universidad de la Republica)
- Agencia Nacional de Investigacion e Innovacion (ANII)
Ammonia-oxidizing microorganisms are partly responsible for the production of N2O, a potent greenhouse gas. Rice paddies provide a habitat where ammonia oxidizing microorganisms can be active. The influence of different agricultural practices on these microorganisms, particularly on archaeal ammonia oxidizers, is an active field of research. In this work, we conducted two greenhouse experiments where we analyzed the influence of two soil types with different organic matter contents, two rice cultivars and water management on both archaeal (AOA) and bacterial (AOB) ammonia oxidizers. We determined the AOA and AOB abundance and population structure by q-PCR and T-RFLP, respectively. The archaeal and bacterial ammonia monooxygenase subunit A gene was used as the PCR target. The AOA and AOB copy numbers were affected by sampling time in both experiments. AOA abundance was also influenced by the time of flooding. The population structure of AOA was more variable than that of AOB and was strongly determined by soil type. Changes in AOB population structure were observed mainly according to sampling time. (C) 2015 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据