期刊
JOURNAL OF IMMUNOLOGY
卷 203, 期 2, 页码 429-440出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1801546
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资金
- National Natural Science Foundation of China [31672592, 31811540395]
- National Key Research and Development Program [2017YFD0500900, 2017YFD0501100, 2016YFE0204100]
- Central Public Interest Scientific Institution Basal Research Fund [1610312016002, 1610312018003, Y2017JC57]
- Elite Youth Program of the Chinese Academy of Agricultural Sciences
Foot-and-mouth disease virus (FMDV) is highly infectious and causes a major plague in animal farming. Unfolded protein response is one of the major cellular responses to pathogenic infections, which performs a crucial role in cell survival, apoptosis, and antiviral innate immune response. In this study, we showed that FMDV infection activated two unfolded protein response branches (PERK-eIF2 alpha and ATF6 signaling) in both baby hamster kidney cells (BHK-21) and porcine kidney (PK-15) cells, whereas it suppressed the IRE1 alpha-XBP1 signaling by decreasing IRE1 alpha level. Further study revealed IRE1 alpha signaling as an important antiviral innate immune mechanism against FMDV. Sec62, the transport protein, was greatly decreased at the late stages of FMDV infection. By overexpression and knockdown study, we also found that the expression of Sec62 was positively involved in the levels of IRE1 alpha and RIG-I and subsequent activation of downstream antiviral signaling pathways in FMDV-infected PK-15 cells. Taken together, our study demonstrates that Sec62 is an important antiviral factor that upregulates IRE1 alpha-RIG-I-dependent antiviral innate immune responses, and FMDV evades antiviral host defense mechanism by downregulating Sec62-IRE1 alpha/RIG-I.
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