Article
Multidisciplinary Sciences
Takashi Iizuka, Kousho Wakae, Masanori Ono, Takuma Suzuki, Yasunari Mizumoto, Kouichi Kitamura, Shin-ichi Horike, Masamichi Muramatsu, Hiroshi Fujiwara
Summary: The study revealed that AID plays a significant role in regulating the expression of GDF-9 and SCF in the ovary, possibly through a GDF-9 and SCF feedback system to modulate communication between oocytes and granulosa cells.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Xia Xie, Tingting Gan, Bing Rao, Weiwei Zhang, Rohit A. Panchakshari, Dingpeng Yang, Xiong Ji, Yu Cao, Frederick W. Alt, Fei-Long Meng, Jiazhi Hu
Summary: The mutant AID-delC protein fails to efficiently target antibody genes, forms condensates, and may exert a dominant-negative effect on wild-type AID.
Review
Genetics & Heredity
Tony M. Mertz, Christopher D. Collins, Madeline Dennis, Margo Coxon, Steven A. Roberts
Summary: The occurrence and development of cancer are closely related to mutations, and dysregulated activity of APOBECs can lead to mutations. The study of mutation signatures helps us understand the patterns and processes of mutations. In humans, APOBEC-generated genetic heterogeneity plays an important role in cancer development, metastasis, and resistance to therapy.
ANNUAL REVIEW OF GENETICS
(2022)
Article
Medicine, General & Internal
Shohei Kikuchi, Akinori Wada, Yusuke Kamihara, Yoshimi Nabe, Tomoki Minemura, Jun Murakami, Nam H. H. Dang, Tsutomu Sato
Summary: Biclonal gammopathy is a rare phenomenon characterized by the detection of 2 M proteins in the same patient. The etiology of this condition remains unclear. In this study, we found double positive cells in two cases of B-cell malignancy with biclonal gammopathy, suggesting a possible involvement of cell transition and class switch recombination. Immunostaining revealed the expression of activation induced cytidine deaminase in these cells, indicating its potential role in the pathogenesis of biclonal gammopathy.
Article
Biochemistry & Molecular Biology
Asami Nishikori, Yoshito Nishimura, Rei Shibata, Koh-ichi Ohshima, Yuka Gion, Tomoka Ikeda, Midori Filiz Nishimura, Tadashi Yoshino, Yasuharu Sato
Summary: AID expression in IgG4-related diseases is influenced by various factors, and AID may play a key role in driving fibrosis and oncogenesis in ocular adnexal organs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
Kairen Tian, Xia Hong, Manman Guo, Yanni Li, Hao Wu, Qinggele Caiyin, Jianjun Qiao
Summary: This study reports the development of a CRISPR-based multi-loci editing system that can efficiently edit multiple loci in L. lactis, providing a powerful tool for industrial applications.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Oncology
Neeraj Sharma, James B. Smadbeck, Nadine Abdallah, Cinthya Zepeda-Mendoza, Moritz Binder, Kathryn E. Pearce, Yan W. Asmann, Jess F. Peterson, Rhett P. Ketterling, Patricia T. Greipp, P. Leif Bergsagel, S. Vincent Rajkumar, Shaji K. Kumar, Linda B. Baughn
Summary: Structural variants of the MYC gene region are common in multiple myeloma and influence disease progression. Different MYC SVs show varying prognostic significance, with non-Ig insertion subtype associated with improved outcomes and Ig insertion subtype, specifically involving the IgL gene partner, associated with poorer outcomes. Next-generation sequencing should be considered for a more comprehensive evaluation of MYC SVs in multiple myeloma.
CLINICAL CANCER RESEARCH
(2021)
Article
Genetics & Heredity
Yuki Murakami, Kei Kimura-Masuda, Tsukasa Oda, Ikuko Matsumura, Yuta Masuda, Rei Ishihara, Saki Watanabe, Yuko Kuroda, Tetsuhiro Kasamatsu, Nanami Gotoh, Hisashi Takei, Nobuhiko Kobayashi, Takayuki Saitoh, Hirokazu Murakami, Hiroshi Handa
Summary: MicroRNAs (miRNAs and miRs) play a key role in modulating gene expression, and their dysregulation has been implicated in multiple myeloma (MM). This study focused on the interaction between MYC and the TP53-miR34 axis in MM and found that MYC participates in the suppression of p53-dependent miRNA expressions. The inhibition of miRNA expression leads to MM development and malignant transformation.
Article
Biochemistry & Molecular Biology
Ramin Sakhtemani, Madusha L. W. Perera, Daniel Huebschmann, Reiner Siebert, Michael S. Lawrence, Ashok S. Bhagwat
Summary: Activation-induced deaminase (AID) and human APOBEC3A were found to preferentially target tRNA genes and transcription start sites, but do not show preference for highly transcribed genes. Unlike other enzymes, AID also lacks replicative strand bias or a preference for hairpin loops when causing mutations.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Baole Qu, Yun Zhao, Lili An, Haiying Hang
Summary: This study demonstrates that adenine deaminase (ADA) can efficiently induce random A/T to G/C mutations on the target gene in CHO cell display, and can be applied in affinity maturation to improve antibody affinity. The combined use of ADA and activation-induced cytidine deaminase (AID) provides more mutant types, offering an opportunity to acquire new mutants with higher affinities than those obtained by only using AID.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Oncology
Rosine Onclercq-Delic, Geraldine Buhagiar-Labarchede, Sophie Leboucher, Thibaut Larcher, Mireille Ledevin, Christelle Machon, Jerome Guitton, Mounira Amor-Guitton
Summary: Cytidine deaminase (CDA) catalyzes the deamination of cytidine (C) and deoxycytidine (dC) to uridine and deoxyuridine, respectively. CDA deficiency leads to genomic instability, and we investigated its role in cancer development using a mouse model. While Cda-/- mice did not differ from Cda+/+ mice in lifetime phenotypic or behavioral characteristics, or in the frequency or type of spontaneous cancers, they had a significantly lower frequency of chemically induced colon tumors. Our results suggest that an absence of Cda limits chemically induced tumors.
Article
Microbiology
Feixuan Li, Xiao-Yu Liu, Lei Ni, Fan Jin
Summary: Researchers developed a method called AIDmut-Seq to detect transcription factor targets on the genome. It involves simple steps and has been validated for various transcriptional activators. However, it has lower efficiency for some small transcriptional repressors. Despite potential false-positive and false-negative results, AIDmut-Seq shows promise as a complementary tool for studying protein-DNA interactions.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Marta Larrayoz, Maria J. Garcia-Barchino, Jon Celay, Amaia Etxebeste, Maddalen Jimenez, Cristina Perez, Raquel Ordonez, Cesar Cobaleda, Cirino Botta, Vicente Fresquet, Sergio Roa, Ibai Goicoechea, Catarina Maia, Miren Lasaga, Marta Chesi, P. Leif Bergsagel, Maria J. Larrayoz, Maria J. Calasanz, Elena Campos-Sanchez, Jorge Martinez-Cano, Carlos Panizo, Paula Rodriguez-Otero, Silvestre Vicent, Giovanna Roncador, Patricia Gonzalez, Satoru Takahashi, Samuel G. Katz, Loren D. Walensky, Shannon M. Ruppert, Elisabeth A. Lasater, Maria Amann, Teresa Lozano, Diana Llopiz, Pablo Sarobe, Juan J. Lasarte, Nuria Planell, David Gomez-Cabrero, Olga Kudryashova, Anna Kurilovich, Maria V. Revuelta, Leandro Cerchietti, Xabier Agirre, Jesus San Miguel, Bruno Paiva, Felipe Prosper, Jose A. Martinez-Climent
Summary: The lack of preclinical models reflecting the genetic heterogeneity of multiple myeloma (MM) has hindered therapeutic discoveries. To overcome this limitation, researchers used genetically engineered mice to develop bone marrow tumors that mimic MM pathogenesis. Integrative analyses of mice and patients revealed a common genetic pathway that accelerates disease progression and immune evasion mechanisms that remodel the bone marrow microenvironment differently.
Review
Immunology
Junna Jiao, Zhuangwei Lv, Yurong Wang, Liye Fan, Angang Yang
Summary: Activation-induced cytidine deaminase (AID) plays a crucial role in promoting B cell diversification through somatic hypermutation (SHM) and class switch recombination (CSR). Apart from its physiological function of humoral immune response, AID has been linked to the initiation and progression of lymphomas. This review proposes an alternative role for AID and explores its off-target effects in regulating tumorigenesis.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yelena Britan-Rosich, Jing Ma, Eran Kotler, Faizan Hassan, Alexander Botvinnik, Yoav Smith, Ofra Moshel, Abed Nasereddin, Gunjan Sharma, Eli Pikarsky, Susan Ross, Moshe Kotler
Summary: APOBEC3G (A3G) promotes repair of DNA double-strand breaks (DSBs) and facilitates error-free rejoining, in addition to its role in immunity. Inhibiting A3G may improve the efficacy of genotoxic therapies, while enhancing A3G activity may reduce acute radiation syndrome.
Article
Oncology
Xinfang Yu, Wei Li, Haidan Liu, Qipan Deng, Xu Wang, Hui Hu, Zijun Y. Xu-Monette, Wei Xiong, Zhongxin Lu, Ken H. Young, Wei Wang, Yong Li
JOURNAL OF HEMATOLOGY & ONCOLOGY
(2020)
Article
Oncology
Hui Zhou, Zijun Y. Xu-Monette, Ling Xiao, Paolo Strati, Fredrick Hagemeister, Yizi He, Huan Chen, Yajun Li, Ganiraju C. Manyam, Yong Li, Santiago Montes-Moreno, Miguel A. Piris, Ken H. Young
BLOOD CANCER JOURNAL
(2020)
Review
Biochemistry & Molecular Biology
Navid Sobhani, Alberto D'Angelo, Xu Wang, Ken H. Young, Daniele Generali, Yong Li
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Hematology
Xinfang Yu, Wei Li, Qipan Deng, Haidan Liu, Xu Wang, Hui Hu, Ya Cao, Zijun Y. Xu-Monette, Ling Li, Mingzhi Zhang, Zhongxin Lu, Ken H. Young, Yong Li
Summary: MYD88(L265P) mutation leads to enhanced NF-κB activation through K63-linked nonproteolytic polyubiquitination catalyzed by RNF138, while A20 mediates proteasomal degradation of RNF138 to suppress NF-κB activation. This mutation-specific biochemical reaction plays a critical role in B-cell oncogenesis, providing a potential therapeutic target for MYD88(L265P) without affecting MYD88(WT) critical for innate immunity.
Review
Biology
Chunmei Fan, Shanshan Zhang, Zhaojian Gong, Xiayu Li, Bo Xiang, Hao Deng, Ming Zhou, Guiyuan Li, Yong Li, Wei Xiong, Zhaoyang Zeng, Xiaoling Li
Summary: The effectiveness of immunotherapies is limited to a small number of cancer patients, with evidence indicating that metabolic reprogramming in the tumor microenvironment affects immune function. Targeting both tumor and immune cell metabolic reprogramming may enhance therapeutic efficacy in antitumor immunotherapies.
SCIENCE CHINA-LIFE SCIENCES
(2021)
Article
Oncology
Chunmei Fan, Hongke Qu, Fang Xiong, Yanyan Tang, Ting Tang, Lishen Zhang, Yongzhen Mo, Xiayu Li, Can Guo, Shanshan Zhang, Zhaojian Gong, Zheng Li, Bo Xiang, Hao Deng, Ming Zhou, Qianjin Liao, Yujuan Zhou, Xiaoling Li, Yong Li, Guiyuan Li, Fuyan Wang, Zhaoyang Zeng
Summary: This study identified a novel circRNA circARHGAP12 that was significantly upregulated in NPC, promoting cell migration and invasion by regulating cytoskeletal remodeling related proteins. CircARHGAP12 was found to directly bind to EZR mRNA and promote its stability, leading to enhanced invasion and metastasis of NPC cells.
Article
Oncology
Evelien Schaafsma, Yanding Zhao, Lanjing Zhang, Yong Li, Chao Cheng
Summary: The study found that MYC activity score accurately reflects MYC pathway activity including various MYC regulatory mechanisms, providing better prognostic predictions in multiple cancer types compared to MYC amplification status, MYC promoter methylation, and MYC mRNA expression. Additionally, tumor proliferation and immune evasion are likely contributors to decreased survival.
MOLECULAR CANCER RESEARCH
(2021)
Article
Biochemical Research Methods
Qingzhou Meng, Xinjie Wang, Yanqun Wang, Lu Dang, Xinyi Liu, Xiaodong Ma, Tian Chi, Xian Wang, Qin Zhao, Guang Yang, Ming Liu, Xingxu Huang, Peixiang Ma
Summary: The study developed a new method called symRNA-Cas12a to detect the D614G mutation in the SARS-CoV-2 spike protein effectively, increasing detection specificity by 13-fold. This method can sensitively detect as low as 10 copies of synthetic mutant RNA.
BIOTECHNOLOGY JOURNAL
(2021)
Article
Oncology
Chunmei Fan, Hongke Qu, Xu Wang, Navid Sobhani, Leiming Wang, Shuanglin Liu, Wei Xiong, Zhaoyang Zeng, Yong Li
Summary: CTAs are a group of tumor antigens expressed in various cancer tissues, with potential as promising candidates for cancer detection and treatment, especially in lung cancer, bladder cancer, and skin cancer.
SEMINARS IN CANCER BIOLOGY
(2021)
Review
Oncology
Navid Sobhani, Dana Rae Tardiel-Cyril, Aram Davtyan, Daniele Generali, Raheleh Roudi, Yong Li
Summary: Immunotherapies have shown promise in cancer treatment but face challenges. Understanding the molecular mechanisms of immune checkpoint inhibitors and the role of regulatory T cells is crucial for improving cancer therapies.
Review
Biochemistry & Molecular Biology
Xinfang Yu, Wei Li, Ken H. Young, Yong Li
Summary: PD-L1 is a classic immune checkpoint molecule with its expression regulated by posttranslational modifications (PTMs) that play vital roles in controlling PD-L1 expression, cellular trafficking, and antitumor immune response.
Article
Oncology
Xu Wang, Xinfang Yu, Wei Li, Praveen Neeli, Ming Liu, Ling Li, Mingzhi Zhang, Xiaosheng Fang, Ken H. Young, Yong Li
Summary: This study demonstrates the significant inhibitory effect of CD38-CAR T cells on various CD38-expressing lymphoid cancer cells and finds that all-trans retinoic acid can increase the expression level of CD38 in low-expressing cancer cells and enhance the anti-tumor activity of daratumumab and CD38-CAR T cells.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Article
Multidisciplinary Sciences
Praveen Neeli, Dafei Chai, Xu Wang, Navid Sobhani, George Udeani, Yong Li
Summary: Emerging variants of SARS-CoV-2 require frequent changes in vaccine antigens. Nucleic acid-based vaccines, such as DNA vaccines, offer flexibility in altering coding sequences and were found to elicit robust immune responses comparable to mRNA vaccines. Adjuvants, such as AS03, and nanoplasmid-based vectors further enhance the immunogenicity of DNA vaccines. These findings highlight the potential of rapid nucleic acid-based vaccine approaches, specifically DNA vaccines, against SARS-CoV-2 and other emerging infectious diseases.
Article
Oncology
Manman Deng, Zijun Y. Xu-Monette, Lan Pham, Xudong Wang, Alexandar Tzankov, Xiaosheng Fang, Feng Zhu, Carlo Visco, Govind Bhagat, Karen Dybkaer, April Chiu, Wayne Tam, Youli Zu, Eric D. Hsi, Hua You, Jooryung Huh, Maurilio Ponzoni, Andres J. M. Ferreri, Michael B. Moller, Benjamin M. Parsons, Fredrick Hagemeister, J. Han van Krieken, Miguel A. Piris, Jane N. Winter, Yong Li, Bing Xu, Phillip Liu, Ken H. Young
Summary: The study indicates that DLBCL/HGBCL patients with MYC/TP53 dual abnormalities exhibit poor clinical outcomes and high-grade morphology, suggesting the need for additional targeted therapies.
MOLECULAR CANCER RESEARCH
(2021)