期刊
JOURNAL OF HAZARDOUS MATERIALS
卷 373, 期 -, 页码 115-121出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jhazmat.2019.03.081
关键词
Photonic; Suspension array; Immunoassay; Antibiotics
资金
- Natural Science Foundation of Jiangsu Province of China [BK20171025]
- National Natural Science Foundation of China [81803274]
- University Natural Science Research Project in Jiangsu Province [16KJB330001, 17KJA620003]
- Postdoctoral Science Foundation of China
- Jiangsu province [2016M601797, 1701182C]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
A multiplex suspension array detection platform of antibiotics has been developed based on silica-agarose hybrid microbeads (SAHMs). Chloramphenicol (CAP), sulfamethoxazole (SMX), metronidazole (MTZ) and amoxicillin (AMX) were employed as model analytes. The antigens (the antibiotics conjugated with BSA) were immobilized on the surface of four different types of SAHMs. Based on an indirect competition immunoassay, the selected antibiotics are detected through the competition of the specific monoclonal antibodies between the multiple antibiotics and the antigens. Due to high resistance to nonspecific protein absorption of SAHMs, the proposed method exhibited wide linear ranges (0.4(similar to)72.9 ng/mL for CAP, 2.0(similar to)108.5 ng/mL for SMX, 2.6(similar to)142.2 ng/mL for MTZ, 1.0(similar to)63.3 ng/mL for AMX) and low detection limits of 0.09(similar to)0.8 ng/mL. Recoveries for spiked tap water samples were from 82% to 113%, with relative standard deviation lower than 14%, demonstrating the accuracy of the measurements performed with the developed method. This work offered a high-throughput, flexible and accurate tool, which provides a good platform for simultaneous detection of antibiotics.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据