Article
Chemistry, Analytical
Pravanjan Malla, Chi-Hsien Liu, Wei-Chi Wu, Pinpinut Kabinsing, Paiboon Sreearunothai
Summary: This article describes a rapid and sensitive electrochemical assay for quantifying the CoV-2 nucleocapsid gene using magnetic nanocomposite and DNA sandwich probes, with a detection limit of 0.37 fM.
Article
Chemistry, Analytical
Daniel Alzate, Maria C. Lopez-Osorio, Fabian Cortes-Mancera, Maria-Cristina Navas, Jahir Orozco
Summary: This study developed a highly specific, sensitive, and portable electrochemical genosensor for detecting HEV genotype 3 in wastewater. By designing specific DNA probes and using biotinylated capture probes and digoxigenin labeled signal probes, the detection of HEV genotype 3 was successfully achieved. The method exhibited high sensitivity, specificity, and reproducibility and could serve as an early warning system for viruses in wastewater.
ANALYTICA CHIMICA ACTA
(2022)
Article
Nanoscience & Nanotechnology
Aisha Javed, Shah Rukh Abbas, Muhammad Uzair Hashmi, Noor Ul Ain Babar, Irshad Hussain
Summary: The study proposes a label-free electrochemical detection method for Mycobacterium tuberculosis (Mtb) using the insertion sequence (IS6110). A graphene oxide-chitosan nanocomposite (GO-CHI) was used to fabricate a positively functionalized electrochemical sensor for Mtb detection, showing high sensitivity and specificity even in raw sputum samples without DNA purification.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Chemistry, Analytical
Jose M. R. Flauzino, Eduardo L. Pimentel, Livia M. Alves, Joao M. Madurro, Ana G. Brito-Madurro
Summary: This work describes the construction of a genosensor based on a graphite electrode modified with a reduced graphene oxide/poly(3-hidroxybenzoic acid) nanocomposite for detection of cattle mitochondrial DNA, ensuring beef purity. The genosensor is selective, reproducible, and stable, capable of identifying even small amounts of pork in beef samples. This genosensor is a valuable tool for evaluating beef purity in meat production and marketing.
Article
Chemistry, Analytical
Sebastian Cajigas, Daniel Alzate, Maritza Fernandez, Carlos Muskus, Jahir Orozco
Summary: This study reports on an electrochemical genosensor specifically designed for the detection and discrimination of SARS-CoV-2. The genosensor demonstrated high sensitivity and specificity, allowing for the detection of SARS-CoV-2 and differentiation from homologous viruses, providing a promising approach for the diagnosis and surveillance of COVID-19.
Review
Chemistry, Analytical
Georgia Balkourani, Theodoros Damartzis, Angeliki Brouzgou, Panagiotis Tsiakaras
Summary: The high conductivity and large surface area of graphene material enhance the sensitivity of non-enzymatic electrochemical sensors. This review classifies the synthesis methods of enzymeless graphene-based glucose electrocatalysts into four main categories and analyzes their electrochemical sensing properties. The mass and charge transport between metal(II)/metal(III) is found to be crucial for glucose electrooxidation mechanism.
Article
Chemistry, Physical
Wei Li Ang, Rachel Rui Xia Lim, Adriano Ambrosi, Alessandra Bonanni
Summary: Discovered in December 2019, the SARS-CoV-2 virus has become a global concern, emphasizing the need for rapid and low-cost detection methods. This study proposes the use of graphene oxide nanocolloids as an electroactive material for the detection of 2019-nCoV genomic sequences. The system shows promise as a simple, sensitive, and cost-effective analytical tool for the detection of the virus.
Article
Biochemistry & Molecular Biology
Jose M. R. Flauzino, Rafaela C. S. Peres, Livia M. Alves, Jussara G. Vieira, Julia G. dos Santos, Ana G. Brito-Madurro, Joao M. Madurro
Summary: Alicyclobacillus acidoterrestris, an acidophilic and thermophilic bacterium found in soil, can affect the quality of fruit juice. This study presents an electrochemical genosensor for detecting A. acidoterrestris, which has potential applications in the juice production chain.
BIOELECTROCHEMISTRY
(2021)
Article
Chemistry, Analytical
Stephanie L. Morais, Piedade Barros, Marlene Santos, Cristina Delerue-Matos, Andreia C. Gomes, M. Fatima Barroso
Summary: This study developed a disposable electrochemical genosensor for detecting toxic dinoflagellate, Alexandrium minutum, by immobilizing a specific DNA probe onto screen-printed gold electrodes. The genosensor showed a concentration range from 0.12 to 1.0 nM with a limit of detection of 24.78 pM and successfully analyzed the targeted genomic DNA from toxic dinoflagellates in the Atlantic Ocean.
Review
Chemistry, Analytical
Lais Canniatti Brazaca, Pamyla Layene dos Santos, Paulo Roberto de Oliveira, Diego Pessoa Rocha, Jessica Santos Stefano, Cristiane Kalinke, Rodrigo Alejandro Abarza Munoz, Juliano Alves Bonacin, Bruno Campos Janegitz, Emanuel Carrilho
Summary: This critical review article discusses the advancements in electrochemical biosensors for diagnosing viral infections, focusing on genosensors, aptasensors, and immunosensors. The article also addresses the advantages of biosensors in viral disease diagnosis and potential future trends in the field, such as lab-on-a-chip and flexible sensors.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Chiye Zhang, Dongmin Shi, Xiaoyuan Li, Jie Yuan
Summary: In this study, a miniaturized electrochemical magneto-immunosensor (EC-MIS) was proposed for portable interleukin-6 (IL-6) detection. The sensitivity of the immuno-sensor was enhanced by electrochemically modifying a micro-fabricated working electrode with a hybrid of reduced graphene oxide (rGO) and gold nanoparticles (AuNPs). The detection of IL-6 was achieved through sandwich immunoassay using magnetic beads attached with the capture antibody, and the sample was restricted and detected on a microfluidic device. The EC-MIS demonstrated a linear range from 0.97 to 250 pg/mL and a limit of detection (LOD) of 0.42 pg/mL.
Article
Biochemistry & Molecular Biology
Emadoddin Amin Sadrabadi, Ali Benvidi, Mostafa Azimzadeh, Leila Asgharnejad, Amin Shiralizadeh Dezfuli, Patricia Khashayar
Summary: In this study, a novel microRNA biosensor based on magnetic rod carbon paste electrodes was developed for breast cancer detection using a relatively new MOF structure as a substrate. The biosensor showed a very low detection limit and a wide dynamic range, indicating its potential for clinical diagnostics.
BIOELECTROCHEMISTRY
(2024)
Article
Engineering, Electrical & Electronic
Zul Arham, Kurniawan Kurniawan, Laode Anhusadar
Summary: The synthesis of new nanomaterial-based modifiers as Pb (II) sensing electrode composites has been a popular research area. In this study, TiO2-incorporated carbon dots (TiO2@C-dots) were synthesized and tested as modifiers in the detection of Pb (II) using a carbon paste electrode (CPE). Various methods were used to fabricate the TiO2@C-dots-decorated CPE (CPE/TiO2@C-dots). The nanocomposites showed uniform solid particle morphology with voids on the surface and exhibited specific IR absorption peaks at wavenumbers of 3600 cm-1 and 1759 cm-1.
MATERIALS SCIENCE IN SEMICONDUCTOR PROCESSING
(2023)
Article
Chemistry, Analytical
Hong Han, Chang Liu, Jingquan Sha, Yu Wang, Chunyao Dong, Mingjun Li, Tiying Jiao
Summary: The ternary nanocomposite of Fc-based carbon nanomaterials with PMo12 is identified as a promising electrochemical sensor for acetaminophen detection, showing low detection limit and wide range of detection capability.
Article
Chemistry, Analytical
Mahboobe Moazampour, Hamid R. Zare, Zahra Shekari, S. Mohammad Moshtaghioun
Summary: The study presents a sensitive electrochemical genosensor for detecting miR-200a using ferrocene-functionalized graphene oxide nanosheets as an electroactive label. A glassy carbon electrode was modified with amino-functionalized CuO nanoparticles decorated on MnO2 nanorods. The genosensor construction was confirmed using electrochemical impedance spectroscopy. The genosensor exhibited the ability to detect miR-200a in a concentration range of 1.0 fM to 0.1 μM with a detection limit of 0.29 fM. It also demonstrated the capability to detect single-base mismatches and non-complementary sequences in the target DNA.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Sandro H. D. Ribeiro, Livia M. Alves, Jose M. R. Flauzino, Anna C. R. Moco, Monica S. Segatto, Jonathan P. Silva, Lizandra F. A. Borges, Joao M. Madurro, Ana G. B. Madurro
Article
Chemistry, Analytical
Jose M. R. Flauzino, Eduardo L. Pimentel, Livia M. Alves, Joao M. Madurro, Ana G. Brito-Madurro
Summary: This work describes the construction of a genosensor based on a graphite electrode modified with a reduced graphene oxide/poly(3-hidroxybenzoic acid) nanocomposite for detection of cattle mitochondrial DNA, ensuring beef purity. The genosensor is selective, reproducible, and stable, capable of identifying even small amounts of pork in beef samples. This genosensor is a valuable tool for evaluating beef purity in meat production and marketing.
Article
Biochemistry & Molecular Biology
Jose M. R. Flauzino, Rafaela C. S. Peres, Livia M. Alves, Jussara G. Vieira, Julia G. dos Santos, Ana G. Brito-Madurro, Joao M. Madurro
Summary: Alicyclobacillus acidoterrestris, an acidophilic and thermophilic bacterium found in soil, can affect the quality of fruit juice. This study presents an electrochemical genosensor for detecting A. acidoterrestris, which has potential applications in the juice production chain.
BIOELECTROCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Ana Cristina Honorato de Castro, Leandro Toshio Kochi, Jose Manuel Rodrigueiro Flauzino, Marcia Maria Costa Nunes Soares, Valeria Almeida Alves, Luis Antonio da Silva, Joao Marcos Madurro, Ana Graci Brito-Madurro
Summary: In this study, a novel electrochemical device was developed for molecular diagnosis of hepatitis B virus in the blood plasma of infected patients. The device showed high sensitivity, good selectivity, low sample volume, and fast assay time, making it a promising tool for diagnosing hepatitis B virus in blood plasma.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Article
Biophysics
Jose M. R. Flauzino, Emily P. Nguyen, Qiuyue Yang, Giulio Rosati, David Panacek, Ana G. Brito-Madurro, Joao M. Madurro, Aristides Bakandritsos, Michal Otyepka, Arben Merkoci
Summary: This study presents a label-free impedimetric genosensor for the sensitive detection of pork residues in meat, utilizing the biosensing capabilities of graphene acid. The sensor can detect pork residues in beef in less than 45 minutes, with a detection limit of 9%.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Anna C. R. Moco, Joao A. S. Neto, Dayane D. de Moraes, Pedro H. Guedes, Jessica G. Brussasco, Jose M. R. Flauzino, Luiz F. G. Luz, Marcia M. C. N. Soares, Joao M. Madurro, Ana G. Brito-Madurro
Summary: This study presents a new methodology for constructing screen-printed electrodes for the detection of HIV RNA, utilizing carbon ink and modified gold nanoparticles. The biosensor demonstrated sensitivity in detecting HIV RNA within the clinically relevant concentration range, allowing for quantification of viral load.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Pedro H. G. Guedes, Jessica G. Brussasco, Anna C. R. Moco, Dayane D. Moraes, Jose M. R. Flauzino, Luiz F. G. Luz, Margarete T. G. Almeida, Marcia M. C. N. Soares, Ronaldo J. Oliveira, Joao M. Madurro, Ana G. Brito-Madurro
Summary: This study developed a genosensor using a gold electrode and ninhydrin to detect C. auris gDNA with high sensitivity and selectivity, demonstrating potential for accurate detection in biological samples. The genosensor can be reused multiple times and maintained 100% response rate even after 80 days of storage.
Article
Chemistry, Multidisciplinary
Ana C. H. Castro, Italo R. S. Bezerra, Aline M. Pascon, Gabriela H. da Silva, Eric A. Philot, Vivian L. de Oliveira, Rodrigo S. N. Mancini, Gabriel R. Schleder, Carlos E. Castro, Luciani R. S. de Carvalho, Bianca H. V. Fernandes, Eduardo M. Cilli, Paulo R. S. Sanches, Murilo Santhiago, Ives Charlie-Silva, Diego S. T. Martinez, Ana L. Scott, Wendel A. Alves, Renato S. Lima
Summary: In this study, a peptide sequence that can recognize S protein antibodies with higher sensitivity was synthesized by mimicking the molecular recognition of antibodies against the SARS-CoV-2 virus. A label-free biosensor was developed by adsorbing the peptide sequence onto an electrode, enabling fast and simple analysis. The peptide was found to bind to the antibody through hydrogen bonds and hydrophobic interactions, and exhibited good stability. With the help of machine learning, this sensor achieved accurate differentiation between COVID-19 infected and healthy samples.
Article
Biochemistry & Molecular Biology
Jessica G. Brussasco, Pedro H. G. Guedes, Anna C. R. Moco, Dayane D. Moraes, Jose M. R. Flauzino, Heliane S. Silva, Atair C. Silva, Jonathan P. Silva, Joao M. Madurro, Ana G. Brito-Madurro
Summary: This study reports the construction of a novel nanostructured immunosensor for detection of troponin I biomarker. The immunosensor exhibited a wide work range and low detection limit, as well as good selectivity and specificity in real samples. Furthermore, the immunosensor showed the possibility of reuse and featured a simplified and inexpensive platform, demonstrating excellent potential for application in the early diagnosis of acute myocardial infarction.
JOURNAL OF MOLECULAR RECOGNITION
(2023)
Review
Food Science & Technology
Jose M. R. Flauzino, Livia M. Alves, Vinicius R. Rodovalho, Joao M. Madurro, Ana G. Brito Madurro
Summary: Biosensors have made significant advances in the meat industry, particularly in pathogen detection and freshness analysis. They are also being used to detect adulteration in meat by different animal species. However, the majority of studies focus on optical biosensors, with pork being the most commonly detected species. These devices often require extensive purification and amplification steps, making them unsuitable for point-of-need use. More robust devices are needed to be developed for use at any stage of the meat industrial chain.
Article
Chemistry, Multidisciplinary
Jose M. R. Flauzino, Martin-Alex Nalepa, Demetrios D. Chronopoulos, Veronika Sedajova, David Panacek, Petr Jakubec, Petra Kuhrova, Martin Pykal, Pavel Banas, Ales Panacek, Aristides Bakandritsos, Michal Otyepka
Summary: A novel graphene derivative equipped with alkyne groups was prepared and used as a platform for constructing an aptasensor for the selective determination of ampicillin. The electrochemical aptasensor displayed a low detection limit, high selectivity among other antibiotics, and showed good performance in real samples.
Article
Veterinary Sciences
Fabiana de Almeida Araujo Santos, Patricia Tiemi Fujimura, Emilia Rezende Vaz, Ana Cristina Honorato de Castro, Ana Graci Brito-Madurro, Vivian Alonso-Goulart, Luciana Machado Bastos, Belchiolina Beatriz Fonseca, Anna Monteiro Correia Lima, Luiz Ricardo Goulart
Summary: This study demonstrates the effectiveness of mimetic epitopes of B. abortus in developing new platforms for diagnosing brucellosis. Additionally, a fast test based on an electrochemical sensor using graphite modified with poly-3-hydroxyphenylacetic acid is proposed.
VETERINARY RESEARCH COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
Pedro H. G. Guedes, Jessica G. Brussasco, Anna C. R. Moco, Dayane D. Moraes, Monica Segatto, Jose M. R. Flauzino, Ana P. Mendes-Silva, Carlos U. Vieira, Joa M. Madurro, Ana G. Brito-Madurro
Summary: Late-Life Depression (LLD) is a common psychiatric disorder in elderly individuals, leading to significant impairment in functioning. Down-regulation of miR-184 (hsa-miR-184) expression can serve as a biomarker for LLD. This study introduces a novel electrochemical genosensor for the rapid diagnosis of LLD based on the detection of miR-184 in plasma using differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS).
Article
Chemistry, Analytical
Freddy A. Nunez, Ana C. H. Castro, Isabela P. Daher, Edecio Cunha-Neto, Jorge Kalil, Silvia B. Boscardin, Alexandre J. C. Lanfredi, Vivian L. de Oliveira, Wendel A. Alves
Summary: The evaluation of serological responses to COVID-19 is crucial for population-level surveillance, developing new vaccines, and evaluating the efficacy of different immunization programs. A biosensor based on immobilization of the recombinant S protein on ZnONRs was employed for serological evaluation. The technology shows great potential for vaccine assessment and monitoring seroprevalence.