Article
Multidisciplinary Sciences
Hailong He, Christine Schoenmann, Mathias Schwarz, Benedikt Hindelang, Andrei Berezhnoi, Susanne Annette Steimle-Grauer, Ulf Darsow, Juan Aguirre, Vasilis Ntziachristos
Summary: This study demonstrates the use of fast scanning raster-scan optoacoustic mesoscopy (RSOM) to achieve high-resolution imaging of melanoma vasculature in humans. By extracting biomarkers from RSOM imaging, it is possible to distinguish between malignant and benign lesions, thus improving diagnostics.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Adrian Buehler, Emma Brown, Lars-Philip Paulus, Markus Eckstein, Oana-Maria Thoma, Mariam-Eleni Oraiopoulou, Ulrich Rother, Andre Hoerning, Arndt Hartmann, Markus F. F. Neurath, Joachim Woelfle, Oliver Friedrich, Maximilian J. J. Waldner, Ferdinand Knieling, Sarah E. E. Bohndiek, Adrian P. P. Regensburger
Summary: Optoacoustic imaging enables microscale imaging of endogenous chromophores and has shown potential in identifying superficial microvascular changes associated with human skin pathologies. Raster-scanning optoacoustic mesoscopy has been used to image vascular structures in the gastrointestinal tract in animal models, but accurate localization of intra-abdominal organs is still challenging. This study presents the development of a novel transrectal absorber guide for optoacoustic mesoscopy to enable accurate transabdominal localization and assessment of colonic vascular networks. The potential of this technique is demonstrated through its application in colitis models in mice, highlighting its potential as a monitoring tool for inflammatory bowel diseases.
Article
Acoustics
Xiuting Li, Mohesh Moothanchery, Cheng Yi Kwa, Wan Ling Tan, Yik Weng Yew, Steven Tien Guan Thng, U. S. Dinish, Amalina Binte Ebrahim Attia, Malini Olivo
Summary: Atopic dermatitis is a chronic disease that significantly impacts healthcare management and patient quality of life. Using ms-RSOM imaging, we found a correlation between oxygen saturation and blood volume with clinical scoring for assessing the severity of AD. Additionally, we observed a relationship between epidermal thickness and disease severity.
Article
Acoustics
T. Nau, C. Schoenmann, B. Hindelang, L. Riobo, A. Doll, S. Schneider, L. Englert, H. He, T. Biedermann, U. Darsow, F. Lauffer, V. Ntziachristos, J. Aguirre
Summary: Atopic dermatitis (AD) is a common chronic inflammatory skin disease. Current severity assessments rely on visual observation and subjective symptoms. However, optoacoustic mesoscopy has been shown to provide precise biomarkers of inflammation, potentially enabling more accurate evaluations of AD severity.
Article
Dermatology
Emily L. Geisler, Andrew Brannen, Mark Pressler, Jeyna Perez, Alex A. Kane, Rami R. Hallac
Summary: This study explores the clinical potential of raster-scanning optoacoustic mesoscopy (RSOM) in the treatment of vascular anomalies. By scanning and calculating blood vessel volume, it was found that CM showed an increase in volume after treatment, while IH showed a decrease. These findings suggest that RSOM may contribute to developing an objective method for evaluating vascular lesions.
LASERS IN SURGERY AND MEDICINE
(2022)
Article
Dermatology
Tassahil Messas, Achraf Messas, George Kroumpouzos
Summary: Optoacoustic imaging (OAI) is a hybrid imaging technique that combines optical contrast and ultrasound detection. Raster-scan optoacoustic mesoscopy (RSOM) is an emerging OAI method that provides information about the structure, function, and molecular features of dermatological conditions. Several studies have shown the usefulness of RSOM in inflammatory skin conditions such as psoriasis and eczema, particularly in quantifying changes to the dermal vasculature. RSOM can also aid in the early diagnosis and monitoring of disease activity and treatment efficacy in psoriasis. Multispectral RSOM, which uses multiple wavelengths of light, is beneficial in diagnosing and monitoring hypoxia-associated conditions such as systemic sclerosis and chronic wounds. OAI techniques can visualize the pathological vascularization of skin cancers, differentiate them from normal skin, measure the depth of malignant melanoma, and detect metastatic spread to lymph nodes. This review highlights the wide range of potential applications of OAI imaging, especially RSOM, in the diagnosis, treatment, and management of skin diseases.
CLINICS IN DERMATOLOGY
(2022)
Article
Biochemical Research Methods
U. S. Dinish, Yik Weng Yew, Keertana Vinod Ram, Renzhe Bi, Amalina Binte Ebrahim Attia, Valerie Teo Xinhui, Poongkulali Rajarahm, Hazel Hweeboon Oon, Steven Tien Guan Thng, Malini Olivo
Summary: A handheld non-invasive confocal Raman system (CRS) was used to evaluate the differences in skin biochemicals between atopic dermatitis (AD) and psoriasis. The relative amount of water was found to decrease in the sequence of healthy skin, psoriasis, and AD. Differential trends were observed for subclasses of ceramides, with ceramide 3 being lower in lesional AD and psoriasis skin, while ceramide 2 showed a contrasting trend. The amount of cholesterol was significantly higher in lesional psoriasis.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Chemistry, Analytical
Kapil Dev, Chris Jun Hui Ho, Renzhe Bi, Yik Weng Yew, U. S. Dinish, Amalina Binte Ebrahim Attia, Mohesh Moothanchery, Steven Thng Tien Guan, Malini Olivo
Summary: This study used confocal Raman micro-spectroscopy and advanced machine learning methods to classify eczema patients and healthy controls with high sensitivity and specificity. By analyzing the Raman spectra of the skin surface, the important role of stratum corneum proteins and lipids in distinguishing subject groups was elucidated.
Article
Dermatology
Bruce Strober, Usha G. Mallya, Min Yang, Sohini Ganguli, Abhijit Gadkari, Jessie Wang, Debra Sierka, Dimittri Delevry, Alexa B. Kimball
Summary: This study evaluated self-reported disease control and quality of life in patients with atopic dermatitis (AD) after initiating dupilumab treatment. Results showed significant improvements in disease control, treatment satisfaction, skin symptoms, and quality of life over the 12-month period, with consistent findings between observed data and imputed data using pattern mixture models for missing data.
Article
Dermatology
Alexa B. Kimball, Dimittri Delevry, Min Yang, Chien-Chia Chuang, Zhixiao Wang, Gaelle Bego-Le-Bagousse, Bruno Martins, Eric Wu, Brad Shumel, Jessie Wang, Debra Sierka, Jingdong Chao, Bruce Strober
Summary: This study evaluated the long-term effectiveness of dupilumab treatment for atopic dermatitis from the patients' perspective. The results showed that after 30-36 months of treatment, patients experienced continued improvement in symptoms, treatment satisfaction, and quality of life, while reducing the need for other treatments.
DERMATOLOGY AND THERAPY
(2023)
Article
Pediatrics
Colin A. Goebel, Emma Brown, Fabian B. Fahlbusch, Alexandra L. Wagner, Adrian Buehler, Thomas Raupach, Martin Hohmann, Moritz Spaeth, Neal Burton, Joachim Woelfle, Michael Schmidt, Andrea Hartner, Adrian P. Regensburger, Ferdinand Knieling
Summary: In this study, label-free raster scanning optoacoustic mesoscopy (RSOM) was used to visualize and quantify murine renal vasculature in explanted organs. The results demonstrated the capability of RSOM for high-resolution imaging of vascular morphology in ex vivo murine renal tissue, suggesting its potential for further preclinical and clinical imaging applications.
MOLECULAR AND CELLULAR PEDIATRICS
(2022)
Article
Dermatology
Andrew Blauvelt, Emma Guttman-Yassky, Amy S. Paller, Eric L. Simpson, Michael J. Cork, Jamie Weisman, John Browning, Weily Soong, Xian Sun, Zhen Chen, Matthew P. Kosloski, Mohamed A. Kamal, Dimittri Delevry, Chien-Chia Chuang, John T. O'Malley, Ashish Bansal
Summary: This study reports the long-term safety and efficacy of dupilumab in adolescents with moderate-to-severe atopic dermatitis. It shows that long-term treatment with dupilumab provides incremental clinical benefit while maintaining an acceptable safety profile. The optimal dosing regimen for this age group may be every 2 weeks, and continued treatment is necessary to maintain efficacy.
AMERICAN JOURNAL OF CLINICAL DERMATOLOGY
(2022)
Review
Medicine, General & Internal
Jiacai Cho, Joanne Lee, Ching-Hui Sia, Chieh Sian Koo, Benjamin Y. Q. Tan, Weizhen Hong, Ellie Choi, Xueying Goh, Louis Chai, Nisha Suyien Chandran, Horng Ruey Chua, Bernard Chan, Mark Muthiah, Ting Ting Low, Eng Soo Yap, Manjari Lahiri
Summary: This study aimed to describe the extrapulmonary manifestations of SARS-CoV-2 infection, including their frequency, onset, pathogenesis, and association with disease severity. The study found that early manifestations included olfactory and gustatory disturbance, gastrointestinal symptoms, and rash, while later manifestations included multiorgan complications and thromboembolic events. Uncommon complications such as Guillain-Barre syndrome, rhabdomyolysis, otitis media, meningoencephalitis, and spontaneous pneumomediastinum were also identified. In conclusion, there are two phases of extrapulmonary disease in SARS-CoV-2 infection, with the need for a multidisciplinary consensus to approach and define thromboinflammation and cytokine release syndrome.
SINGAPORE MEDICAL JOURNAL
(2023)
Letter
Dermatology
Ellie C. E. Choi, Li Wei Heng, Sean Y. L. Tan, Phillip H. C. Phan, Nisha Suyien Chandran
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY
(2022)
Article
Medicine, General & Internal
Sean Tan, Phillip Phan, Je Yin Law, Ellie Choi, Nisha Suyien Chandran
Summary: This study aimed to explore the phenomenon of topical corticosteroid (TCS) phobia and understand the factors driving concerns about TCS, particularly regarding steroid addiction and withdrawal. The study found that patients' attitudes towards TCS, availability of alternatives, treatment inconvenience, personality, patient's ongoing evaluation of clinical response to TCS, doctor-patient relationship, and healthcare-seeking behavior were the main factors influencing TCS concerns.
Article
Acoustics
Zakiullah Ali, Christian Zakian, Qian Li, Jerome Gloriod, Sophie Crozat, Francois Bouvet, Guillaume Pierre, Vassilis Sarantos, Massimiliano Di Pietro, Krzysztof Flisikowski, Peter Andersen, Wolfgang Drexler, Vasilis Ntziachristos
Summary: A 360-degree, 50 Hz frame rate scanning capsule optoacoustic endoscope for human gastrointestinal tract imaging is reported in this study. The imaging penetration depth and translational potential of the instrument were validated using an intralipid solution to simulate light scattering in human esophageal tissue and a pig esophagus.
Article
Acoustics
Nikolina-Alexia Fasoula, Angelos Karlas, Olga Prokopchuk, Nikoletta Katsouli, Michail Bariotakis, Evangelos Liapis, Anna Goetz, Michael Kallmayer, Josefine Reber, Alexander Novotny, Helmut Friess, Marc Ringelhan, Roland Schmid, Hans -Henning Eckstein, Susanna Hofmann, Vasilis Ntziachristos
Summary: This study investigates whether multispectral optoacoustic tomography (MSOT) can detect and monitor hepatic steatosis by analyzing the spectral region around 930 nm. In a pilot study involving patients and mice, higher absorptions at 930 nm were observed in liver steatosis cases compared to healthy individuals, while no significant difference was observed in subcutaneous adipose tissue. This study suggests that MSOT has the potential to be a non-invasive and portable technique for detecting and monitoring hepatic steatosis in clinical settings, warranting further studies.
Review
Chemistry, Analytical
Baocheng Li, Ruochong Zhang, Renzhe Bi, Malini Olivo
Summary: Biosensing and bioimaging play a crucial role in understanding biological and pathological processes, and optical fiber has emerged as a promising technology in these fields. This review provides an overview of seven common types of optical fiber biosensors and optical fiber-based ultrasound detection in photoacoustic imaging (PAI), discussing their applications and potential in biosensing and bioimaging. Furthermore, the challenges and future directions in this area are briefly discussed.
Article
Chemistry, Analytical
Yi Qi, Ruochong Zhang, Poongkulali Rajarahm, Shuyan Zhang, Amalina Binte Ebrahim Attia, Renzhe Bi, Malini Olivo
Summary: In this study, a portable confocal Raman spectroscopy system with a simultaneous dual-wavelength source and a miniaturized handheld probe was developed. The system can acquire spectra in both fingerprint and high wavenumber regions simultaneously. An innovative design and a unique Raman spectra separation algorithm were implemented to improve the efficiency and accuracy of spectra acquisition.
ANALYTICAL CHEMISTRY
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Milou E. Noltes, Maximilian Bader, Madelon J. H. Metman, Jasper Vonk, Pieter J. Steinkamp, Jan Kukacka, Henriette E. Westerlaan, Rudi A. J. O. Dierckx, Bettien M. van Hemel, Adrienne H. Brouwers, Gooitzen M. van Dam, Dominik Juestel, Vasilis Ntziachristos, Schelto Kruijff
Summary: This study examines the potential of multispectral optoacoustic tomography (MSOT) for risk stratification of thyroid nodules. The findings show a correlation between vascular patterns and thyroid malignancy, and identify malignant features in MSOT images that are verified by histopathology. However, mean oxygen saturation does not provide significant differences between benign and malignant thyroid nodules. Recommendations are made to consider optoacoustic features and chromophore quantification together for malignancy risk assessment.
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2023)
Article
Biochemical Research Methods
Philipp Rauschendorfer, Georg Wissmeyer, Farouc A. Jaffer, Dimitris Gorpas, Vasilis Ntziachristos
Summary: This article introduces a new method for attenuation correction in intravascular near-infrared fluorescence (NIRF) imaging. It utilizes a fluorophore-coated guidewire as a reference to measure blood attenuation in real-time during the NIRF examination. The method has been shown to significantly improve signal quality and accuracy in animal experiments.
JOURNAL OF BIOMEDICAL OPTICS
(2023)
Article
Multidisciplinary Sciences
Konxhe Kulaj, Alexandra Harger, Michaela Bauer, Oezuem S. Caliskan, Tilak Kumar Gupta, Dapi Menglin Chiang, Edward Milbank, Josefine Reber, Angelos Karlas, Petra Kotzbeck, David N. Sailer, Francesco Volta, Dominik Lutter, Sneha Prakash, Juliane Merl-Pham, Vasilis Ntziachristos, Hans Hauner, Michael W. Pfaffl, Matthias H. Tschoep, Timo D. Mueller, Stefanie M. Hauck, Benjamin D. Engel, Jantje M. Gerdes, Paul T. Pfluger, Natalie Krahmer, Kerstin Stemmer
Summary: Extracellular vesicles (EVs) derived from adipocytes transfer insulinotropic proteins to pancreatic β-cells, enhancing insulin secretion and glucose tolerance. This process is observed in insulin-resistant obese mice, suggesting that AdEVs play a role in informing β-cells about insulin resistance and increasing insulin demand.
NATURE COMMUNICATIONS
(2023)
Article
Nanoscience & Nanotechnology
Hailong He, Ludwig Englert, Vasilis Ntziachristos
Summary: Optical endoscopy is widely used for gastrointestinal diagnostics, but it only allows superficial inspection of the mucosa. To visualize deeper layers, optoacoustic endoscopy has been developed, which combines optical contrast with ultrasound to provide high-resolution imaging. This review focuses on the progress, current status, and potential applications of optoacoustic endoscopy.
Article
Acoustics
Angelos Karlas, Nikolina-Alexia Fasoula, Nikoletta Katsouli, Michael Kallmayer, Sabine Sieber, Sebastian Schmidt, Evangelos Liapis, Martin Halle, Hans-Henning Eckstein, Vasilis Ntziachristos
Summary: By using multispectral optoacoustic tomography (MSOT), this study imaged hemodynamics and oxygen kinetics within skeletal muscle during exercise. The results showed changes in blood volume and oxygen levels during different types of hand-grip exercises in healthy volunteers. Further analysis revealed insights into arteriovenous oxygen differences and muscle oxygen consumption. These findings highlight the potential implications of MSOT in muscle research and disease diagnostics.
Review
Chemistry, Analytical
Shuyan Zhang, Yi Qi, Sonia Peng Hwee Tan, Renzhe Bi, Malini Olivo
Summary: Molecular vibrations are essential in physical chemistry and biochemistry, and Raman and infrared spectroscopy are the primary techniques used for vibrational spectroscopy. These techniques provide distinct molecular fingerprints that can be used to identify chemical bonds, functional groups, and molecular structures. This review article explores recent advancements in using Raman and infrared spectroscopy for molecular fingerprint detection, focusing on identifying specific biomolecules and understanding the chemical composition of biological samples for cancer diagnosis. The article also discusses the working principles and instrumentation of each technique, highlighting the analytical versatility of vibrational spectroscopy.
Article
Chemistry, Analytical
Yi Qi, Ruochong Zhang, Poongkulali Rajarahm, Shuyan Zhang, Amalina Binte Ebrahim Attia, Renzhe Bi, Malini Olivo
Summary: In this study, a portable confocal Raman spectroscopy (CRS) system with a simultaneous dual-wavelength source and a miniaturized handheld probe was developed. A novel algorithm was also developed for accurate spectral separation. The results demonstrated that the proposed CRS system and algorithm greatly improve the integration of CRS in the clinical workflow.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Isaac Huen, Ruochong Zhang, Renzhe Bi, Xiuting Li, Mohesh Moothanchery, Malini Olivo
Summary: This study analyzed the performance of different preprocessing methods in photoacoustic tomography (PAT). Bandpass filtering was found to be the most effective denoising method, and considering directivity significantly reduced noise. It also improved lateral resolution in some cases.
