Article
Food Science & Technology
Yi Lin, Wentao Yu, Chunping Cai, Pengjie Wang, Shuilian Gao, Jianming Zhang, Xiaojing Fan, Wanping Fang, Naixing Ye
Summary: This study used microfluidic technology and SNP biomarkers to authenticate the varieties of oolong tea products, and found that the most frequent adulterant of Tieguanyin oolong tea on the market is Benshan. It also reported for the first time the commercial behavior of using Fuyun6 and Jinguanyin as adulterants or contamination in the production of Tieguanyin oolong tea. This research proposes rapid authentication technology for oolong tea to promote consumer trust and food quality supervision.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biochemical Research Methods
Karthick Mani, Chia-Yuan Chen
Summary: The study revealed the effects of loud background noise exposure on zebrafish larvae and proposed a non-invasive method for sorting/trapping hatched larvae in a microfluidic environment. This method has potential applications in drug screening by efficiently transporting and sorting zebrafish larvae into specific chambers of a microchannel network.
Article
Multidisciplinary Sciences
Ewa Sieliwonczyk, Bert Vandendriessche, Charlotte Claes, Evy Mayeur, Maaike Alaerts, Philip Holmgren, Tycho Canter Cremers, Dirk Snyders, Bart Loeys, Dorien Schepers
Summary: Despite challenges, researchers have developed a novel method to improve knock-in efficiency using CRISPR-Cas9 technique in zebrafish. By combining the early selection procedure with genotyping and next-generation sequencing, they achieved a significant increase in somatic editing efficiency and demonstrated potential for improving germline transmission in pre-selected embryos.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Samuel K. H. Sy, Danny C. W. Chan, Roy C. H. Chan, Jing Lyu, Zhongqi Li, Kenneth K. Y. Wong, Chung Hang Jonathan Choi, Vincent C. T. Mok, Hei-Ming Lai, Owen Randlett, Yu Hu, Ho Ko
Summary: Fish-on-Chips is an optofluidic tool that allows for precise chemical cue presentation, behavioral response monitoring, and whole-brain neuronal activity recording in larval zebrafish. It utilizes laminar fluid flows to control chemical delivery and enables the investigation of neural coding in the chemical senses. The platform has demonstrated its strength in revealing behavioral strategies and neural representations of odor sensing in the vertebrate model.
NATURE COMMUNICATIONS
(2023)
Article
Biochemical Research Methods
Arezoo Khalili, Ellen Wijngaarden, Khaled Youssef, Georg R. Zoidl, Pouya Rezai
Summary: By improving the microfluidic device, we successfully increased the number of zebrafish larvae specimens from one to four, with improved efficiency and throughput. The new design reduced testing time per larva and increased loading and orientation efficiencies.
BIOTECHNOLOGY JOURNAL
(2022)
Article
Engineering, Electrical & Electronic
Arezoo Khalili, Ellen van Wijngaarden, Georg R. Zoidl, Pouya Rezai
Summary: Through experiments using a microfluidic device, we found that changing the direction of electric current and voltage magnitude significantly affected the locomotion response of zebrafish larvae. Repeated exposure to electric pulses led to a diminishing response in the larvae, but the response could be fully recovered after a period of rest or introduction of a new stimulus, indicating habituation as a form of non-associative learning.
SENSORS AND ACTUATORS A-PHYSICAL
(2021)
Article
Multidisciplinary Sciences
Martina Venditti, Catia Pedalino, Marion Rosello, Giulia Fasano, Malo Serafini, Celine Revenu, Filippo Del Bene, Marco Tartaglia, Antonella Lauri
Summary: Current genetic modification and phenotyping methods in teleost fish have greatly advanced genetic research and drug testing, with the small zebrafish becoming a popular alternative to large animals' experimentation. A minimally invasive method using tail fin scratching allows for early genotyping and reduces the number of animals used in mutant line generation.
SCIENTIFIC REPORTS
(2022)
Article
Engineering, Biomedical
Gefei Zhang, Mingsi Tong, Songlin Zhuang, Xinghu Yu, Weichao Sun, Weiyang Lin, Huijun Gao
Summary: This study presents a novel zebrafish larva manipulation technique that achieves high success rate and reduces damage by orienting and aspirating zebrafish larvae. The method offers advantages of low cost, easy implementability, and good stability.
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING
(2021)
Article
Nanoscience & Nanotechnology
Karthick Mani, Chia-Yuan Chen
Summary: This study introduces a smart microfluidic device that enables efficient temperature control, remote zebrafish transport, perfusion, and dynamic culturing all together, providing the potential for on-chip high-quality imaging.
MICROFLUIDICS AND NANOFLUIDICS
(2021)
Article
Chemistry, Analytical
Satishkumar Subendran, Chun-Wei Kang, Chia-Yuan Chen
Summary: The study utilized zebrafish as a reliable species for biomechanical-related investigations, focusing on quantifying the hydrodynamic flow physics caused by tail beating behaviors to better understand food toxicity and potential human responses. A novel microfluidic device coupled with a shape memory alloy actuator was designed to immobilize zebrafish for hydrodynamic quantification, aiding in assessing zebrafish as an animal model for new drug discovery and development. The research provided valuable insights into early developmental behavioral patterns of zebrafish and its implications on drug discovery.
Article
Biology
Alireza Zabihihesari, Arezoo Khalili, Arthur J. Hilliker, Pouya Rezai
Summary: This study quantified the heartbeat parameters of small model organisms, fruit fly and zebrafish, in-vivo using microfluidics and a novel MATLAB-based software. The software automatically detected heartbeats, extracted heartbeat signals, and removed noise, enabling quantification of various heart parameters in intact larvae. This method provides a new approach for research in cardiac physiology of model organisms.
COMPUTERS IN BIOLOGY AND MEDICINE
(2021)
Article
Neurosciences
Annelies Van Dyck, Luca Masin, Steven Bergmans, Giel Schevenels, An Beckers, Benoit Vanhollebeke, Lieve Moons
Summary: Unlike mammals, adult zebrafish can fully regenerate axons and recover from neuronal damage in the central nervous system. By studying optic nerve injury-induced axonal regrowth in zebrafish, researchers have observed dendritic remodeling and changes in mitochondrial dynamics, which contribute to effective axonal and dendritic repair. A novel microfluidic model has been developed to investigate these interactions and explore how redirecting energy resources supports successful regeneration.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2023)
Article
Multidisciplinary Sciences
Haisong Lin, Wenzhuo Yu, Kiarash A. Sabet, Michael Bogumil, Yichao Zhao, Jacob Hambalek, Shuyu Lin, Sukantha Chandrasekaran, Omai Garner, Dino Di Carlo, Sam Emaminejad
Summary: This study presents a swarm of millimeter-sized magnet robots ('ferrobots') that can handle magnetized sample droplets and deliver nucleic acid amplification tests with precision and reliability. The automated platform demonstrated laboratory-equivalent operations for pooled testing, maximizing testing efficiency. Applied for SARS-CoV-2 virus detection, the results matched those obtained off-chip. The technology is easily manufacturable and distributable, potentially reducing reagent costs and instrumentation cost, making it a promising solution to expand global testing capacity.
Article
Developmental Biology
Ji Yun Jang, Ting Liang, Myeong-Kyu Kim, Kyung Wook Kang, Bora Lee, Seok-Yong Choi
Summary: Genotyping involves analysis of PCR products using genomic DNA as template, and is used to validate mutant or transgenic organisms. We developed a rapid and noninvasive method for extracting PCR-ready gDNA from adult zebrafish by combining skin swabbing with proteinase K treatment.
Article
Biochemical Research Methods
Brian J. O'Grady, Michael D. Geuy, Hyosung Kim, Kylie M. Balotin, Everett R. Allchin, David C. Florian, Neelansh N. Bute, Taylor E. Scott, Gregory B. Lowen, Colin M. Fricker, Matthew L. Fitzgerald, Scott A. Guelcher, John P. Wikswo, Leon M. Bellan, Ethan S. Lippmann
Summary: Depositing a thin layer of parylene on 3D printed models allows them to be used as master molds for elastomeric device fabrication, making the prints biocompatible. Uncoated 3D prints can release toxic materials causing cell death, while parylene-coated prints can maintain cell viability and healthy morphology. This technique is simple and applicable for traditional microdevice fabrication and rapid prototyping of new designs.
Review
Andrology
Cathy L. Mangum, Darshan P. Patel, Alexander R. Jafek, Raheel Samuel, Tim G. Jenkins, Kenneth I. Aston, Bruce K. Gale, James M. Hotaling
TRANSLATIONAL ANDROLOGY AND UROLOGY
(2020)
Article
Instruments & Instrumentation
Pratima Labroo, Scott Ho, Himanshu Sant, Jill E. Shea, Jayant Agarwal, Bruce Gale
Summary: By developing a mathematical model and conducting experimental validation, the diffusion of drugs in a hole-based drug delivery nerve conduit was successfully studied, with results indicating the reliability and functionality of the model. It was observed that drug release in the conduit can be tuned by changing device parameters, with the model error being less than 10%.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2021)
Review
Biochemical Research Methods
Harikrishnan Jayamohan, Christopher J. Lambert, Himanshu J. Sant, Alexander Jafek, Dhruv Patel, Haidong Feng, Michael Beeman, Tawsif Mahmood, Ugochukwu Nze, Bruce K. Gale
Summary: The unprecedented global pandemic of SARS-CoV-2 has pushed the limits of modern viral diagnostics, highlighting both the advantages and limitations of current technologies while prompting efforts to expand diagnostic capacity. Key observations in functionality of current SARS-CoV-2 diagnostic methods have been put forth, with emphasis on critical aspects such as sample collection, testing models, and commercial responses. Integrated nature of assays is stressed, where issues in sample collection and preparation could impact overall performance in a clinical setting.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Farhad Shiri, Bruce K. Gale, Himanshu Sant, Brody King, Gina T. Bardi, Joshua L. Hood, Kevin E. Petersen
Summary: A new high-volume, continuous particle separation device based on size and charge has been developed, showing improved particle separations over traditional devices. This new technique combines electrical and flow fields into a single device, streamlining high-throughput continuous separations based upon electrophoretic mobility.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Kevin Petersen, Farhad Shiri, Tonguc Onur Tasci, Himanshu Sant, Joshua Hood, Bruce Gale
Summary: The simulation of a novel Fl-El-SPLITT separation device was described and validated using existing theory and experimentation for the first time. The simulation accurately predicted the experimentally obtained transition points and trends seen in AC testing, showing the influence of DC, AC, and crossflows on the separation of polystyrene particles.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Engineering, Biomedical
John Nelson, Dev Patel, Himanshu J. Sant, Jill Shea, Bruce K. Gale, Jay Agarwal
Summary: This study investigated the relationship between vessel wall compression and friction fit strength to ensure reducing coupler size would not increase the risk of failure. Results showed that different compressive strains and materials can impact the strength of friction fit under varying conditions.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2021)
Article
Engineering, Biomedical
Susan Wojtalewicz, Jonathon Vizmeg, Sierra Erickson, Caleb Lade, Jill Shea, Himanshu Sant, Jules Magda, Bruce Gale, Jayant Agarwal, Brett Davis
Summary: This study evaluated the material properties of a novel local anesthetic delivery system for controlled release of bupivacaine for 72 hours. The results showed that hot homogenized particles had a slower and more controlled release, and hyaluronic acid hydrogel was important for lowering injection forces and minimizing clogging events.
JOURNAL OF BIOMATERIALS APPLICATIONS
(2022)
Article
Multidisciplinary Sciences
Farhad Shiri, Haidong Feng, Kevin E. Petersen, Himanshu Sant, Gina T. Bardi, Luke A. Schroeder, Michael L. Merchant, Bruce K. Gale, Joshua L. Hood
Summary: Nanoscale and microscale cell-derived extracellular vesicles types and subtypes have significant importance in biology and medicine. Researchers have developed a miniaturized device that uses elasto-inertial effect to separate different types of extracellular vesicles, which can be applied in microfluidic applications. The device has successfully re-isolated and recovered extracellular vesicles in the experiment.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Haidong Feng, Alexander R. Jafek, Bonan Wang, Hayden Brady, Jules J. Magda, Bruce K. Gale
Summary: This paper studies particle focusing in viscoelastic flow and predicts the focusing position by analyzing the force balance. The research finds that particle separation resolution can be improved in viscoelastic flows.
Article
Chemistry, Analytical
Dhruv Patel, Harikrishnan Jayamohan, Ugochukwu Nze, Christopher Lambert, Haidong Feng, Tawsif Mahmood, Bruce Gale, Himanshu Sant
Summary: Pathogen detection in food is crucial for community health and economic impact. Current detection systems face challenges in pathogen extraction and release from food matrices. This study introduces a method combining stomaching with cavitation to enhance pathogen extraction from ground beef and improve downstream detection of E. coli O157:H7.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Haidong Feng, Dhruv Patel, Jules J. Magda, Sage Geher, Paul A. Sigala, Bruce K. Gale
Summary: This paper observes the transition from single-stream focusing to multiple-stream focusing in high viscoelastic flow and investigates the influence of cell physical properties on the formation of multiple-streams focusing. Particle separation based on different criteria is achieved, and it demonstrates the feasibility of separating red and white blood cells as well as malaria-infected red blood cells using this method.
Article
Engineering, Environmental
Zhiqiang Tan, Weichen Zhao, Yongguang Yin, Ming Xu, Wenxiao Pan, Yanwanjing Liu, Qinghua Zhang, Bruce K. Gale, Yukui Rui, Jingfu Liu
Summary: This study evaluated the separation ability of biased cyclical electrical field-flow fractionation (BCyElFFF) for polyvinyl pyrrolidone-coated and poly(ethylene glycol)-coated silver nanoparticles (AgNPs) with different electrophoretic mobilities, and elucidated the mechanism behind the formation of NOM corona on these AgNP surfaces. Furthermore, it was observed that the survival rates of E. coli exposed to AgNPs with or without NOM corona collected during repeated BCyElFFF runs increased with increasing NOM concentration, indicating the negative effect of NOM coronas on the bioavailability of AgNPs. These findings highlight the powerful capability of BCyElFFF in studying the fate and transformation of nanoparticles in aqueous environments.
Article
Chemistry, Multidisciplinary
Christopher J. Lambert, Harikrishnan Jayamohan, Bruce K. Gale, Lars B. Laurentius, Dhruv Patel, Madison Hansen, Tawsif Mahmood, Himanshu Jayant Sant
Summary: This study presents an electrochemical immunoassay-based method for the precise, simple, quick, and cost-effective detection of SARS-CoV-2 nucleocapsid protein. By immunomagnetic capture of the protein in nasal samples, followed by electrochemical detection using gold nanoparticle labels on a screen-printed carbon electrode, the researchers determined optimal conditions to maximize the electrochemical signal and successfully detected the nucleocapsid protein in SARS-CoV-2-positive samples with a low viral load.
APPLIED SCIENCES-BASEL
(2023)
Article
Biochemical Research Methods
Matt D. Nelson, Brady L. Goenner, Bruce K. Gale
Summary: ChatGPT is a generative AI model that has attracted significant attention due to its advanced reasoning and analysis capabilities. It can solve various problems by creating and debugging code. In this study, GPT-4 was utilized to design functional microfluidic components using the open-source CAD software OpenSCAD through iterative dialogue. This concept has the potential to advance CAD usage for both technical and non-technical users and can be extended to other fields.
Article
Chemistry, Analytical
Haidong Feng, Alex Jafek, Raheel Samuel, James Hotaling, Timothy G. Jenkins, Kenneth I. Aston, Bruce K. Gale
Summary: A high recovery, high throughput sperm separation process is proposed for clinical biopsy sperm retrieval, achieving a 95.6% sperm recovery with 87.4% removal of non-sperm cells. The chance of finding sperm in treated samples increases by 8.2 fold, supporting the development of a rapid biopsy sperm sorting process. The mechanism can also be applied for high-efficiency separation of non-spherical particles in general.