Review
Biochemistry & Molecular Biology
Manizha Shekibi, Sophea Heng, Guiying Nie
Summary: The development of endometrial receptivity is crucial for successful embryo implantation and pregnancy initiation. Recent studies have focused on the role of microRNAs (miRNAs) in the regulation of endometrial receptivity. This review provides an up-to-date knowledge of the miRNAs identified in humans and mice that may play a functional role in the establishment of receptivity. The findings suggest that miRNAs could be potential biomarkers and therapeutic targets for improving endometrial receptivity in human fertility treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Hon Yeung Chan, Ha M. Tran, James Breen, John E. Schjenken, Sarah A. Robertson
Summary: This study provides a comprehensive profile of the endometrial transcriptome in the peri-ovulatory and peri-implantation states, identifying genes, regulatory pathways, and upstream drivers involved in the transition to uterine receptivity that were not previously associated with implantation. Several upstream regulators, including cytokines, ovarian steroid hormones, prostaglandin E2, and vascular endothelial growth factor A, were found to be activated during this transition. The findings of this study will contribute to further research on the molecular mechanisms of uterine receptivity.
Article
Medicine, Research & Experimental
Danbi Lee, Yu-Gyeong Lee, Jieun Won, Seon-Hwa Hong, Jee Hyun Kim, Youn-Jung Kang
Summary: This study aimed to investigate the optimal timing of hCG administration to enhance embryo implantation rates. The results showed that hCG administration induced a significant increase in endometrial receptivity and angiogenesis-related gene expression levels within 72 hours, and promoted the stability of embryo attachment in an in vitro model.
Article
Biochemistry & Molecular Biology
Xiao Ma, Jing Wang, Likai Wang, Laiqing Yan, Yunjie Liu, Wenkui Ma, Pengyun Ji, Lu Zhang, Guoshi Liu
Summary: Using Aanat and Mt2 KO mice, this study found that the melatonergic system plays a crucial role in early pregnancy in mice. The deficiency of AANAT and MT2 resulted in reduced implantation sites and abnormal endometrium morphology. Mechanistically, the melatonergic system is essential for normal endometrial receptivity and functions by activating the STAT signaling pathway. Melatonin production reduction and signal transduction impairment led to hyperproliferative endometrium and increased local immunoinflammatory reaction, causing early abortion in Mt2 KO mice. These findings might have implications in other animals, including humans.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Obstetrics & Gynecology
Sarah G. Paule, Sophea Heng, Nirukshi Samarajeewa, Ying Li, Mary Mansilla, Andrew Webb, Thomas Nebl, Steven L. Young, Bruce A. Lessey, M. Louise Hull, Maxine Scelwyn, Rebecca Lim, Beverley Vollenhoven, Luk J. Rombauts, Guiying Nie
Summary: This study identified PODX as a key negative regulator of endometrial receptivity, with its specific downregulation in the mid-secretory phase likely mediated by progesterone. Overexpression of PODX inhibited human embryo attachment and invasion in functional models, highlighting its role as a negative regulator of epithelial receptivity for implantation.
HUMAN REPRODUCTION
(2021)
Article
Environmental Sciences
Yuxiang Liang, Qizhi Shuai, Ying Wang, Shanshan Jin, Zihan Feng, Binghong Chen, Ting Liang, Zhizhen Liu, Hong Zhao, Zhaoyang Chen, Chunfang Wang, Jun Xie
Summary: The study reveals that 1-NP has adverse effects on embryo implantation in mice and endometrial receptivity in humans, possibly achieved through inhibition of signaling pathways, receptor expression, and cell proliferation.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Obstetrics & Gynecology
Jigal Haas, Robert F. Casper
Summary: This brief review examines the investigation of the endometrial cavity before embryo transfer using various techniques, including hysteroscopy, endometrial biopsy, and ultrasound imaging. These investigative tools are currently controversial and require large prospective controlled trials for validation. Retained embryos during embryo transfer do not appear to have a negative impact on pregnancy outcome, and physical activity immediately after embryo transfer has no impact on pregnancy outcome, according to consistent data.
FERTILITY AND STERILITY
(2022)
Article
Developmental Biology
Jacqueline Sehring, Angeline Beltsos, Roohi Jeelani
Summary: This review discusses the complexity of human embryo implantation, including the window of implantation, cyclical phases of the endometrium, the implantation process itself, and features of endometrial receptivity. Additionally, it explores new research on inflammatory reproductive biology, epigenetics and microRNA, and the role of the vaginal and endometrial microbiome in implantation.
Article
Endocrinology & Metabolism
Li Wang, Shulan Lv, Fen Li, E. Bai, Xiaofeng Yang
Summary: This study compared endometrial receptivity during implantation windows among letrozole, clomiphene citrate, and natural cycle in polycystic ovary syndrome (PCOS) patients. The results showed that letrozole had superior endometrial receptivity compared to clomiphene citrate, leading to higher clinical pregnancy and ongoing pregnancy rates. Endometrial flow index during the implantation window was found to have the highest predictive value for pregnancy.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Obstetrics & Gynecology
Sophea Heng, Nirukshi Samarajeewa, Asma Aberkane, Wafaa Essahib, Hilde Van de Velde, Maxine Scelwyn, M. Louise Hull, Beverley Vollenhoven, Luk J. Rombauts, Guiying Nie
Summary: This study found that PCX inhibits embryo implantation in vitro and in IVF patients. High levels of PCX significantly inhibited blastocyst attachment and penetration. The presence of PCX in the luminal epithelium was negatively associated with the success rate of clinical pregnancy.
FERTILITY AND STERILITY
(2021)
Article
Genetics & Heredity
Tiantian Li, Ellen M. Greenblatt, Michelle EyunJung Shin, Theodore J. Brown, Crystal Chan
Summary: Immunohistochemical measurement of endometrial LAMB3 expression could be a promising prognostic method to predict pregnancy outcome for RIF patients undergoing FETs. The H-score cutoff of 4.129 achieved a 75% accuracy in distinguishing positive or negative pregnancy outcomes. In subsequent FET cycles, patients with a mock cycle LAMB3 H-score >= 4.129 had a three-fold higher pregnancy rate compared to those <4.129.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Review
Reproductive Biology
Erika Elinor Paulson, Pierre Comizzoli
Summary: The review aims to summarize the current data on the mechanisms involved in uterine receptivity and embryo implantation in carnivores and identify research priorities to better understand a key phenomenon in a critical group of mammals.
BIOLOGY OF REPRODUCTION
(2021)
Article
Endocrinology & Metabolism
Marina Segura-Benitez, Maria Cristina Carbajo-Garcia, Ana Corachan, Amparo Faus, Antonio Pellicer, Hortensia Ferrero
Summary: Successful implantation relies on communication between maternal endometrium and embryo, with EVs playing a role in this process. Ultracentrifugation was found to be the most efficient method for EV isolation from endometrial epithelial cells, with EVs containing proteins related to embryo implantation processes.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2022)
Review
Cell Biology
Sepide Goharitaban, Ali Abedelahi, Kobra Hamdi, Mozafar Khazaei, Masoumeh Esmaeilivand, Behrooz Niknafs
Summary: This review discusses the potential use of endometrial miRNAs as noninvasive biomarkers to predict the implantation and reproductive ability of the endometrium during in vitro fertilization (IVF) cycles. Previous studies have shown that certain miRNA levels in the endometrium are associated with implantation potential and pregnancy outcomes. This review introduces the possibility of using endometrial miRNAs as noninvasive biomarkers in assisted reproductive technology (ART) cycles to determine the optimal time for implantation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Endocrinology & Metabolism
Jing Yang, Linlin Wang, Jingwen Ma, Lianghui Diao, Jiao Chen, Yanxiang Cheng, Longfei Li
Summary: This study identified four potential biomarkers (TPPP3, S100A13, HSD17B2, and AZGP1) associated with non-receptive endometrium using iTRAQ-based proteomics analysis. It was found that TPPP3 and HSD17B2 were downregulated in the endometrium of patients with poor endometrial receptivity. These findings provide new evidence for the diagnosis and treatment of non-receptive endometrium and recurrent implantation failure (RIF).
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Medicine, General & Internal
Yu Yang, Dandan Zhang, Huamin Qin, Shuai Liu, Qiu Yan
Article
Cell Biology
Xinyuan Cui, Hao Wang, Yaqi Li, Tianhong Chen, Shuai Liu, Qiu Yan
CELL PROLIFERATION
(2020)
Article
Reproductive Biology
Yu Yang, Juan Liang, Siyi Chen, Aihui Zhang, Yaqi Li, Shuai Liu, Qiu Yan
Summary: Endometrial decidualization plays a critical role in uterine receptivity and embryo implantation, and dysregulation of decidualization is associated with pregnancy-related disorders like miscarriage. Protein glycosylation, specifically O-fucosylation by poFUT1, is involved in various physiological processes. In this study, the researchers discovered that BMP1, an essential glycoprotein in reproduction, undergoes O-fucosylation. The levels of poFUT1 and BMP1 were higher in the secretory phase and during early pregnancy, but decreased in the decidua of miscarriage patients. Moreover, the O-fucosylation of BMP1 by poFUT1 promoted decidualization by enhancing BMP1 secretion and activation of the BMP/Smad signaling pathway.
BIOLOGY OF REPRODUCTION
(2023)