Article
Engineering, Biomedical
Eric Parigoris, Soojung Lee, David Mertz, Madeleine Turner, Amy Y. Liu, Jason Sentosa, Sabra Djomehri, Hao Chen Chang, Kathryn Luker, Gary Luker, Celina G. Kleer, Shuichi Takayama
Summary: This study describes mammary organoids with a basal-in phenotype, achieved through specific material considerations and co-culture of cells, showing self-organization of cancer and epithelial cells inside the organoid. It was found that invasion of tumor cells is more prominent in organoids with less developed basement membranes compared to those with more defined ones.
ADVANCED HEALTHCARE MATERIALS
(2021)
Review
Biochemistry & Molecular Biology
Nargess Khalilgharibi, Yanlan Mao
Summary: The basement membrane plays a crucial role in tissue development and diseases, with its complex mechanical properties changing through different molecular mechanisms, thereby affecting tissue form and function. Investigating the mechanical properties of the basement membrane is important for disease diagnosis and treatment, as well as the development of better in silico and in vitro models, which can also reduce the use of animals in research.
Article
Multidisciplinary Sciences
Hui Li, Yue Zheng, Yu Long Han, Shengqiang Cai, Ming Guo
Summary: By conducting pressure-controlled inflation and deflation experiments, the study directly measured the nonlinear mechanics of basement membranes. It was found that basement membranes act as permeable, hyperelastic materials, exhibiting a remarkable nonlinear stiffening behavior to avoid structural softening and maintain sufficient confining stress to enclosed tissues.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Wenwen Tian, Yongzhou Luo, Yuhui Tang, Yanan Kong, Linyu Wu, Shaoquan Zheng, Chao Zhang, Yutian Zou, Jindong Xie, Xinpei Deng, Junsheng Zhang, Nian Chen, Xiaoming Xie, Weidong Wei
Summary: This study established a prognostic index, BMscore, consisting of six basement membrane genes, and validated its reliability and predictive ability in breast cancer patients. The study also revealed a close correlation between BMscore and immune infiltration and EMT activity in breast cancer.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2023)
Article
Multidisciplinary Sciences
Kazuya Tsujita, Reiko Satow, Shinobu Asada, Yoshikazu Nakamura, Luis Arnes, Keisuke Sako, Yasuyuki Fujita, Kiyoko Fukami, Toshiki Itoh
Summary: Changes in cell mechanics contribute to cancer cell dissemination. High plasma membrane (PM) tension inhibits cancer dissemination by counteracting mechanosensitive BAR family protein assembly, while restoration of PM tension phenotypically convert malignant cells into a non-motile epithelial cell state.
NATURE COMMUNICATIONS
(2021)
Review
Cell Biology
Evelyn Lattmann, Ting Deng, Alex Hajnal
Summary: Cell invasion involves the ability of cells to migrate across basement membranes, crucial in organ development and cancer progression. The switch between cell invasion and proliferation is driven by changes in cellular morphology, with proliferative cells forming contractile rings during cytokinesis while invasive cells form invadopodia to breach the basement membranes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Shayan S. Nazari, Andrew D. Doyle, Christopher K. E. Bleck, Kenneth M. Yamada
Summary: Cancer cells can breach basement-membrane barriers by extending long protrusions, facilitating the initial step of cancer metastasis.
Article
Medical Laboratory Technology
Dun Niu, Ting Luo, Hanbin Wang, Yiniu Xia, Zhizhong Xie
Summary: Tumor invasion is a complex process involving cell-matrix interactions, dysregulated metabolism, and the role of lactic acid. Lactic acid plays a significant role in tumor invasion by impacting extracellular matrix remodeling and epithelial-mesenchymal transition through various pathways.
CLINICA CHIMICA ACTA
(2021)
Article
Oncology
Lixiang Feng, Jun Yang, Wei Zhang, Xiong Wang, Lili Li, Min Peng, Pengcheng Luo
Summary: Based on the importance of the basement membrane (BM) in cancer invasion and metastasis, this study constructed a BM-associated lncRNA risk model for bladder cancer (BCa) patients. The model accurately predicted prognosis, tumor invasion, and metastasis, and showed good predictive power for the tumor microenvironment and mutational burden. The study provides new insights into predicting prognosis and understanding the molecular mechanisms of BCa.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biology
D. Pramanik, M. K. Jolly, R. Bhat
Summary: This study used a computational model to investigate the invasion behavior of malignant epithelial tumor cells in different microenvironments, revealing at least four distinct invasive states. These states were found to be specified by different input signatures of proliferation, adhesion, and extracellular matrix remodeling, and specific input perturbations allowed transitions between the clusters, highlighting variations in the robustness between invasive states. This systems-level approach provides quantitative insights into how the diversity in extracellular matrix microenvironments may influence invasion into diverse phenotypic modes during early dissemination of breast cancer, contributing to tumor heterogeneity.
JOURNAL OF THEORETICAL BIOLOGY
(2021)
Article
Nutrition & Dietetics
Pawel Wronski, Stanislaw Wronski, Marcin Kurant, Bartosz Malinowski, Michal Wicinski
Summary: This article reviews the crucial mechanisms contributing to the invasion and progression of bladder cancer, as well as the inhibitory effect of the natural phytochemical curcumin on bladder cancer growth. The authors particularly focus on the inhibition of matrix metalloproteinases (MMP) by curcumin, which helps maintain basement membrane integrity and alleviate cancer invasion.
Article
Biochemistry & Molecular Biology
Conor J. Sugden, Valentina Iorio, Lee D. Troughton, Ke Liu, Mychel R. P. T. Morais, Rachel Lennon, George Bou-Gharios, Kevin J. Hamill
Summary: Transgenic expression of LaNt alpha 31 in mice resulted in defects in tissue morphogenesis, including extravascular bleeding, kidney epithelial detachment, tubular dilation, disruption of the epidermal basal cell layer and hair follicle outer root sheath, as well as reduced cell numbers in the liver.
Article
Biochemistry & Molecular Biology
Aljona Gaiko-Shcherbak, Julian Eschenbruch, Nils M. Kronenberg, Michael Teske, Benjamin Wolters, Ronald Springer, Malte C. Gather, Rudolf Merkel, Bernd Hoffmann, Erik Noetzel
Summary: The study found that disruption of the basement membrane can lead to the transition of benign acini to invasive, while EGFR activation further compromises the barrier function of the basement membrane, promoting cancer cell invasion. Breast acini continuously sense the mechanical properties of the extracellular matrix by forming new cellular protrusions, pushing and pulling on the basement membrane and extracellular matrix.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Elrade Rofaani, Yong He, Juan Peng, Yong Chen
Summary: This study developed a method to overcome the lack of in vivo-like basement membrane (BM) for in vitro modeling by culturing primordial lung progenitors (PLPs) on an artificial basement membrane (ABM) composed of collagen IV and laminin. The forces generated by the growing epithelial monolayer on the ABM led to geometry-dependent folding and enhanced expression of surfactant protein C and aquaporin 5 compared with a nanofiber-covered substrate. This work emphasizes the importance of recapitulating natural BM for advanced epithelial modeling.
ACTA BIOMATERIALIA
(2023)
Article
Biochemistry & Molecular Biology
Nailah Harvey, Leila Youssefian, Amir Hossein Saeidian, Hassan Vahidnezhad, Jouni Uitto
Summary: Epidermolysis bullosa (EB) is a phenotypically and genetically heterogeneous disorder caused by mutations in genes encoding structural proteins that reinforce skin integrity. Breakdowns in these proteins lead to the disruption of skin adhesion mechanisms. Despite clinical and histopathological confirmation, there are still many genetically unsolved cases of EB. Recent advancements have implicated non-structural proteins in the pathophysiology of EB.
Article
Multidisciplinary Sciences
Hong-pyo Lee, Ryan Stowers, Ovijit Chaudhuri
NATURE COMMUNICATIONS
(2019)
Article
Multidisciplinary Sciences
Joanna Y. Lee, Jessica K. Chang, Antonia A. Dominguez, Hong-pyo Lee, Sungmin Nam, Julie Chang, Sushama Varma, Lei S. Qi, Robert B. West, Ovijit Chaudhuri
NATURE COMMUNICATIONS
(2019)
Article
Engineering, Biomedical
Ryan S. Stowers, Anna Shcherbina, Johnny Israeli, Joshua J. Gruber, Julie Chang, Sungmin Nam, Atefeh Rabiee, Mary N. Teruel, Michael P. Snyder, Anshul Kundaje, Ovijit Chaudhuri
NATURE BIOMEDICAL ENGINEERING
(2019)
Article
Multidisciplinary Sciences
Sungmin Nam, Vivek Kumar Gupta, Hong-pyo Lee, Joanna Y. Lee, Katrina M. Wisdom, Sushama Varma, Eliott Marie Flaum, Ciara Davis, Robert B. West, Ovijit Chaudhuri
Article
Biophysics
Julie Chang, Emily M. Pang, Kolade Adebowale, Katrina M. Wisdom, Ovijit Chaudhuri
BIOPHYSICAL JOURNAL
(2020)
Review
Multidisciplinary Sciences
Ovijit Chaudhuri, Justin Cooper-White, Paul A. Janmey, David J. Mooney, Vivek B. Shenoy
Article
Chemistry, Multidisciplinary
Sungmin Nam, Yung-Hao Lin, Taeyoon Kim, Ovijit Chaudhuri
Summary: In dense collagen gels, single cancer cells generate substantial pushing forces to facilitate cell division without the need for cell spreading or matrix degradation. These forces arise from a combination of interpolar spindle elongation and cytokinetic ring contraction.
Article
Multidisciplinary Sciences
Hong-pyo Lee, Farid Alisafaei, Kolade Adebawale, Julie Chang, Vivek B. Shenoy, Ovijit Chaudhuri
Summary: Cells migrating in confining microenvironments rely on the mechanical generation of migration paths by the nucleus, allowing mesenchymal stem cells to migrate successfully in viscoelastic and plastic hydrogels. The nucleus powers migration by pushing into protrusions, activating mechanosensitive ion channels and increasing osmotic pressure to drive protrusion expansion, rather than limiting migration.
Editorial Material
Engineering, Biomedical
Ryan Stowers, Ovijit Chaudhuri
Summary: The DNA methylation of the oncogenic Yes-associated protein is reversibly regulated by the stiffness of the extracellular matrix.
NATURE BIOMEDICAL ENGINEERING
(2021)
Editorial Material
Biology
Dhiraj Indana, Ovijit Chaudhuri
Summary: A new method for applying solid stress to cell aggregates is revealing the impact of mechanical forces on cancer cells.
Article
Engineering, Biomedical
Joanna Y. Lee, Ovijit Chaudhuri
Summary: The concept of the immune system recognizing and eliminating tumor cells was once controversial, but now forms the foundation of cancer immunology research. New immune-based cancer treatments require complex preclinical models to assess efficacy. Traditional treatments targeting cancer cell growth are no longer sufficient, as the next generation of therapies requires models with greater complexity to simulate immune cell functions.
APL BIOENGINEERING
(2021)
Article
Engineering, Biomedical
Ngan F. Huang, Ovijit Chaudhuri, Patrick Cahan, Aijun Wang, Adam J. Engler, Yingxiao Wang, Sanjay Kumar, Ali Khademhosseini, Song Li
APL BIOENGINEERING
(2020)