E3 ubiquitin-protein ligase TRIM21-mediated lysine capture by UBE2E1 reveals substrate-targeting mode of a ubiquitin-conjugating E2

UbE2E1/UBCH6 Is a Criticalin VivoE2 for the PRC1-catalyzed Ubiquitination of H2A at Lys-119

(2017) Keith Wheaton et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Conformational Dynamics and Allostery in E2:E3 Interactions Drive Ubiquitination: gp78 and Ube2g2

(2017) Kalyan S. Chakrabarti et al.   STRUCTURE

E2 enzymes: more than just middle men

(2016) Mikaela D Stewart et al.   CELL RESEARCH

TRIM9 short isoform preferentially promotes DNA and RNA virus-induced production of type I interferon by recruiting GSK3β to TBK1

(2016) Yunfei Qin et al.   CELL RESEARCH

Functional role of TRIM E3 ligase oligomerization and regulation of catalytic activity

(2016) Marios G Koliopoulos et al.   EMBO JOURNAL

Cryo-EM of Mitotic Checkpoint Complex-Bound APC/C Reveals Reciprocal and Conformational Regulation of Ubiquitin Ligation

(2016) Masaya Yamaguchi et al.   MOLECULAR CELL

Capturing a substrate in an activated RING E3/E2–SUMO complex

(2016) Frederick C. Streich Jr et al.   NATURE

Mechanism of TRIM25 Catalytic Activation in the Antiviral RIG-I Pathway

(2016) Jacint G. Sanchez et al.   Cell Reports

Mechanism of B-box 2 domain-mediated higher-order assembly of the retroviral restriction factor TRIM5α

(2016) Jonathan M Wagner et al.   eLife

Online data analysis at the ESRF bioSAXS beamline, BM29

(2016) Martha Elisabeth Brennich et al.   JOURNAL OF APPLIED CRYSTALLOGRAPHY

TRIM5  requires Ube2W to anchor Lys63-linked ubiquitin chains and restrict reverse transcription

(2015) A. J. Fletcher et al.   EMBO JOURNAL

RING E3-Catalyzed E2 Self-Ubiquitination Attenuates the Activity of Ube2E Ubiquitin-Conjugating Enzymes

(2015) Prerana Agarwal Banka et al.   JOURNAL OF MOLECULAR BIOLOGY

TRIM5α-Mediated Ubiquitin Chain Conjugation Is Required for Inhibition of HIV-1 Reverse Transcription and Capsid Destabilization

(2015) Edward M. Campbell et al.   JOURNAL OF VIROLOGY

Activation of a Primed RING E3-E2–Ubiquitin Complex by Non-Covalent Ubiquitin

(2015) Lori Buetow et al.   MOLECULAR CELL

Structural basis for catalytic activation by the human ZNF451 SUMO E3 ligase

(2015) Laurent Cappadocia et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Sequential ubiquitination and deubiquitination enzymes synchronize the dual sensor and effector functions of TRIM21

(2015) Adam J. Fletcher et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

BMI1–RING1B is an autoinhibited RING E3 ubiquitin ligase

(2015) Asad M. Taherbhoy et al.   Nature Communications

Structural basis for catalytic activation by the human ZNF451 SUMO E3 ligase

(2015) Laurent Cappadocia et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

E2 superfamily of ubiquitin-conjugating enzymes: constitutively active or activated through phosphorylation in the catalytic cleft

(2015) Ilaria Valimberti et al.   Scientific Reports

RING Dimerization Links Higher-Order Assembly of TRIM5α to Synthesis of K63-Linked Polyubiquitin

(2015) Zinaida Yudina et al.   Cell Reports

Fast and Accurate Resonance Assignment of Small-to-Large Proteins by Combining Automated and Manual Approaches

(2015) Markus Niklasson et al.   PLoS Computational Biology

BioSAXS Sample Changer: a robotic sample changer for rapid and reliable high-throughput X-ray solution scattering experiments

(2015) Adam Round et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Structure of a RING E3 Trapped in Action Reveals Ligation Mechanism for the Ubiquitin-like Protein NEDD8

(2014) Daniel C. Scott et al.   CELL

Structural insights into the TRIM family of ubiquitin E3 ligases

(2014) Yang Li et al.   CELL RESEARCH

The E3 Ubiquitin Ligase Ro52 Negatively Regulates IFN-  Production Post-Pathogen Recognition by Polyubiquitin-Mediated Degradation of IRF3

(2014) R. Higgs et al.   JOURNAL OF IMMUNOLOGY

Nucleotide Biosynthetic Enzyme GMP Synthase Is a TRIM21-Controlled Relay of p53 Stabilization

(2014) B. Ashok Reddy et al.   MOLECULAR CELL

New insights into ubiquitin E3 ligase mechanism

(2014) Christopher E Berndsen et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

RING-type E3 ligases: Master manipulators of E2 ubiquitin-conjugating enzymes and ubiquitination

(2013) Meredith B. Metzger et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

TRIMmunity: The Roles of the TRIM E3-Ubiquitin Ligase Family in Innate Antiviral Immunity

(2013) Ricardo Rajsbaum et al.   JOURNAL OF MOLECULAR BIOLOGY

Upgraded ESRF BM29 beamline for SAXS on macromolecules in solution

(2013) Petra Pernot et al.   JOURNAL OF SYNCHROTRON RADIATION

Improved low-resolution crystallographic refinement with Phenix and Rosetta

(2013) Frank DiMaio et al.   NATURE METHODS

Essentiality of a non-RING element in priming donor ubiquitin for catalysis by a monomeric E3

(2013) Hao Dou et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Low-resolution refinement tools inREFMAC5

(2012) Robert A. Nicholls et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

New developments in theATSASprogram package for small-angle scattering data analysis

(2012) Maxim V. Petoukhov et al.   JOURNAL OF APPLIED CRYSTALLOGRAPHY

The immunobiology of Ro52 (TRIM21) in autoimmunity: A critical review

(2012) Vilija Oke et al.   JOURNAL OF AUTOIMMUNITY

A Human Ubiquitin Conjugating Enzyme (E2)-HECT E3 Ligase Structure-function Screen

(2012) Yi Sheng et al.   MOLECULAR & CELLULAR PROTEOMICS

Structure of an E3:E2∼Ub Complex Reveals an Allosteric Mechanism Shared among RING/U-box Ligases

(2012) Jonathan N. Pruneda et al.   MOLECULAR CELL

Structure of a RING E3 ligase and ubiquitin-loaded E2 primed for catalysis

(2012) Anna Plechanovová et al.   NATURE

The E3 ubiquitin ligase TRIM21 negatively regulates the innate immune response to intracellular double-stranded DNA

(2012) Zhiqiang Zhang et al.   NATURE IMMUNOLOGY

BIRC7–E2 ubiquitin conjugate structure reveals the mechanism of ubiquitin transfer by a RING dimer

(2012) Hao Dou et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structural basis for autoinhibition and phosphorylation-dependent activation of c-Cbl

(2012) Hao Dou et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Dynamic Control of Selectivity in the Ubiquitination Pathway Revealed by an ASP to GLU Substitution in an Intra-Molecular Salt-Bridge Network

(2012) Sjoerd J. L. van Wijk et al.   PLoS Computational Biology

Overview of theCCP4 suite and current developments

(2011) Martyn D. Winn et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

A spectrophotometric assay for conjugation of ubiquitin and ubiquitin-like proteins

(2011) Christopher E. Berndsen et al.   ANALYTICAL BIOCHEMISTRY

Functional interactions between ubiquitin E2 enzymes and TRIM proteins

(2011) Luisa M. Napolitano et al.   BIOCHEMICAL JOURNAL

Anti-Ro52 Autoantibodies from Patients with Sjögren's Syndrome Inhibit the Ro52 E3 Ligase Activity by Blocking the E3/E2 Interface

(2011) Alexander Espinosa et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

E2 Conjugating Enzyme Selectivity and Requirements for Function of the E3 Ubiquitin Ligase CHIP

(2011) Sarah E. Soss et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Human Proteome-scale Structural Modeling of E2–E3 Interactions Exploiting Interface Motifs

(2011) Gozde Kar et al.   JOURNAL OF PROTEOME RESEARCH

UBCH7 reactivity profile reveals parkin and HHARI to be RING/HECT hybrids

(2011) Dawn M. Wenzel et al.   NATURE

Neural Stem Cells Maintain Their Stemness Through Protein Kinase C ζ Mediated Inhibition of TRIM32

(2011) Anna-Lena Hillje et al.   STEM CELLS

Features and development ofCoot

(2010) P. Emsley et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

E2s: structurally economical and functionally replete

(2010) Dawn M. Wenzel et al.   BIOCHEMICAL JOURNAL

Molecular Basis for the Association of Human E4B U Box Ubiquitin Ligase with E2-Conjugating Enzymes UbcH5c and Ubc4

(2010) Robert C. Benirschke et al.   STRUCTURE

Ro52-mediated Monoubiquitination of IKK  Down-regulates NF- B Signalling

(2009) K. Wada et al.   JOURNAL OF BIOCHEMISTRY

Loss of the lupus autoantigen Ro52/Trim21 induces tissue inflammation and systemic autoimmunity by disregulating the IL-23–Th17 pathway

(2009) Alexander Espinosa et al.   JOURNAL OF EXPERIMENTAL MEDICINE

TRIM21 Is Essential to Sustain IFN Regulatory Factor 3 Activation during Antiviral Response

(2009) K. Yang et al.   JOURNAL OF IMMUNOLOGY

The autoantigen Ro52 is an E3 ligase resident in the cytoplasm but enters the nucleus upon cellular exposure to nitric oxide

(2008) Alexander Espinosa et al.   EXPERIMENTAL CELL RESEARCH

MULCh: modules for the analysis of small-angle neutron contrast variation data from biomolecular assemblies

(2008) Andrew E. Whitten et al.   JOURNAL OF APPLIED CRYSTALLOGRAPHY